(Circular) (Six-base) MAPSORT of: pFN21AE6375.seq Check: 6733 from: 1 to: 5905 pFN21AE6375 5905 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2622 3045 3715 278 Size: 53 83 186 936 1086 423 670 2468 Fragments arranged by size: 2468 1086 936 670 423 186 83 53 AccI GT'mk_AC Cuts at: 1937 3248 1937 Size: 1311 4594 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2066 2611 2920 3077 3968 5352 5620 1632 Size: 434 545 309 157 891 1384 268 1917 Fragments arranged by size: 1917 1384 891 545 434 309 268 157 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 4804 5781 1203 Size: 3601 977 1327 Fragments arranged by size: 3601 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 3020 4634 3020 Size: 1614 4291 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5905 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5905 AlwNI CAG_nnn'CTG Cuts at: 3113 3648 5220 3113 Size: 535 1572 3798 Fragments arranged by size: 3798 1572 535 ApaLI G'TGCA_C Cuts at: 3962 5118 3962 Size: 1156 4749 ApoI r'AATT_y Cuts at: 1493 3214 3440 3592 5701 1493 Size: 1721 226 152 2109 1697 Fragments arranged by size: 2109 1721 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 2335 3017 3230 1870 Size: 73 392 682 213 4545 Fragments arranged by size: 4545 682 392 213 73 BamHI G'GATC_C Cuts at: 3235 3602 3235 Size: 367 5538 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2236 2551 3139 3226 4681 Size: 325 217 627 449 315 588 87 1455 Cuts at: 4681 618 Size: 1842 Fragments arranged by size: 1842 1455 627 588 449 325 315 217 87 BanII G_rGCy'C Cuts at: 1391 2220 3224 1391 Size: 829 1004 4072 Fragments arranged by size: 4072 1004 829 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2614 3016 3028 3040 928 Size: 971 715 402 12 12 3793 Fragments arranged by size: 3793 971 715 402 12 12 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3145 3179 4096 4130 1903 Size: 34 1208 34 917 34 3678 Fragments arranged by size: 3678 1208 917 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2334 3797 5007 2334 Size: 1463 1210 3232 Fragments arranged by size: 3232 1463 1210 BclI T'GATC_A Cuts at: 1548 2169 2442 2655 2781 1548 Size: 621 273 213 126 4672 Fragments arranged by size: 4672 621 273 213 126 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4516 136 Size: 107 122 71 631 605 2844 1525 Fragments arranged by size: 2844 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 3322 3322 Size: 5905 Bme1580I G_kGCm'C Cuts at: 1790 2556 3142 3966 5122 1790 Size: 766 586 824 1156 2573 Fragments arranged by size: 2573 1156 824 766 586 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3145 3636 4594 Size: 416 53 83 399 24 1728 491 958 Cuts at: 4594 442 Size: 1753 Fragments arranged by size: 1753 1728 958 491 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2265 2914 2942 4565 1239 Size: 887 139 649 28 1623 2579 Fragments arranged by size: 2579 1623 887 649 139 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2234 4030 4895 5193 2234 Size: 1796 865 298 2946 Fragments arranged by size: 2946 1796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2991 3076 4568 882 Size: 617 1492 85 1492 2219 Fragments arranged by size: 2219 1492 1492 617 85 BsaAI yAC'GTr Cuts at: 493 1204 2787 3696 493 Size: 711 1583 909 2702 Fragments arranged by size: 2702 1583 909 711 BsaBI GATnn'nnATC Cuts at: 2660 5899 2660 Size: 3239 2666 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2619 3042 3712 4094 Size: 53 83 186 936 1086 423 670 382 Cuts at: 4094 4729 275 Size: 635 1451 Fragments arranged by size: 1451 1086 936 670 635 423 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4338 4736 5010 5157 5568 4338 Size: 398 274 147 411 4675 Fragments arranged by size: 4675 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2074 2104 1178 Size: 30 165 30 671 30 4979 Fragments arranged by size: 4979 671 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2248 2534 2567 1186 Size: 1062 286 33 4524 Fragments arranged by size: 4524 1062 286 33 BseYI C'CCAG_C Cuts at: 5108 5604 5108 Size: 496 5409 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2618 2687 1163 Size: 573 882 69 4381 Fragments arranged by size: 4381 882 573 69 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3355 4116 5144 1540 Size: 275 186 1354 761 1028 2301 Fragments arranged by size: 2301 1354 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2220 2733 2913 3966 4051 5122 2220 Size: 513 180 1053 85 1071 3003 Fragments arranged by size: 3003 1071 1053 513 180 85 BsmI GAATG_Cn' Cuts at: 3416 3509 3416 Size: 93 5812 BsmBI CGTCTCn'nnnn_ Cuts at: 3128 3128 Size: 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 2556 2733 2913 3142 3224 3966 Size: 399 430 336 177 180 229 82 742 Cuts at: 3966 4051 5122 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 430 399 336 229 180 177 85 82 BspEI T'CCGG_A Cuts at: 5568 5568 Size: 5905 BspHI T'CATG_A Cuts at: 2445 3794 5524 2445 Size: 1349 1730 2826 Fragments arranged by size: 2826 1730 1349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3260 844 Size: 2416 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3792 1394 Size: 429 1969 3507 Fragments arranged by size: 3507 1969 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4400 4574 65 Size: 1183 3152 174 1396 Fragments arranged by size: 3152 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2330 4549 4736 1700 Size: 110 520 2219 187 2869 Fragments arranged by size: 2869 2219 520 187 110 BsrGI T'GTAC_A Cuts at: 96 3072 96 Size: 2976 2929 BssHII G'CGCG_C Cuts at: 1925 3101 1925 Size: 1176 4729 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5905 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5905 BstXI CCAn_nnnn'nTGG Cuts at: 2393 2764 2393 Size: 371 5534 BstYI r'GATC_y Cuts at: 1972 2400 3235 3602 3987 4004 5456 5542 5554 Size: 428 835 367 385 17 1452 86 12 Cuts at: 5554 5900 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 835 428 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2436 2436 Size: 5905 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5353 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5166 Fragments arranged by size: 5166 683 56 BtsI GCAGTG_nn' Cuts at: 2675 2778 3421 4215 4235 2675 Size: 103 643 794 20 4345 Fragments arranged by size: 4345 794 643 103 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4912 809 Size: 507 3596 1802 Fragments arranged by size: 3596 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2144 2692 3352 Size: 54 1425 50 275 18 314 548 660 Cuts at: 3352 4241 5870 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 660 548 314 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3352 1537 Size: 275 1540 4090 Fragments arranged by size: 4090 1540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2747 2807 3835 1353 Size: 1394 60 1028 3423 Fragments arranged by size: 3423 1394 1028 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2473 4492 4866 5012 387 Size: 1064 138 884 2019 374 146 1280 Fragments arranged by size: 2019 1280 1064 884 374 146 138 EcoICRI GAG'CTC Cuts at: 2218 2218 Size: 5905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2126 2265 2611 2914 2920 2942 Size: 393 434 60 139 346 303 6 22 Cuts at: 2942 3077 3968 4565 5352 5620 1239 Size: 135 891 597 787 268 1524 Fragments arranged by size: 1524 891 787 597 434 393 346 303 268 139 135 60 22 6 EcoRI G'AATT_C Cuts at: 3214 3592 3214 Size: 378 5527 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 2370 5052 1678 Size: 692 2682 2531 Fragments arranged by size: 2682 2531 692 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3006 3038 4546 4578 4620 Size: 32 135 32 1434 32 1508 32 42 Cuts at: 4620 4652 1373 Size: 32 2626 Fragments arranged by size: 2626 1508 1434 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3249 3495 669 Size: 988 281 1311 246 3079 Fragments arranged by size: 3079 1311 988 281 246 HindIII A'AGCT_T Cuts at: 748 3145 748 Size: 2397 3508 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2266 5019 5203 1305 Size: 961 2753 184 2007 Fragments arranged by size: 2753 2007 961 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2694 10 Size: 54 1425 343 862 3221 Fragments arranged by size: 3221 1425 862 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2663 2762 3863 4222 4381 518 Size: 648 1497 99 1101 359 159 2042 Fragments arranged by size: 2042 1497 1101 648 359 159 99 MspA1I CmG'CkG Cuts at: 1606 3321 3998 5146 5391 1606 Size: 1715 677 1148 245 2120 Fragments arranged by size: 2120 1715 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5353 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 3263 4808 1132 Size: 97 2034 1545 2229 Fragments arranged by size: 2229 2034 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5905 PciI A'CATG_T Cuts at: 4804 4804 Size: 5905 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5905 PfoI T'CCnGG_A Cuts at: 2242 2913 2242 Size: 671 5234 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PmlI CAC'GTG Cuts at: 2787 2787 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2102 2134 3974 4006 5487 5519 2102 Size: 32 1840 32 1481 32 2488 Fragments arranged by size: 2488 1840 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2126 1613 Size: 513 5392 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2858 2890 2858 Size: 32 5873 PstI C_TGCA'G Cuts at: 830 2773 2796 2949 3257 830 Size: 1943 23 153 308 3478 Fragments arranged by size: 3478 1943 308 153 23 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 SacI G_AGCT'C Cuts at: 2220 2220 Size: 5905 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SapI GCTCTTCn'nnn_ Cuts at: 2747 2747 Size: 5905 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SexAI A'CCwGG_T Cuts at: 2385 2385 Size: 5905 SfcI C'TryA_G Cuts at: 826 1045 2521 2769 2792 2945 3253 4388 5069 Size: 219 1476 248 23 153 308 1135 681 Cuts at: 5069 5260 826 Size: 191 1471 Fragments arranged by size: 1476 1471 1135 681 308 248 219 191 153 23 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 3232 1872 Size: 1360 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2213 4009 4910 5172 820 Size: 197 926 270 1796 901 262 1553 Fragments arranged by size: 1796 1553 926 901 270 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 2203 3829 5 Size: 47 2151 1626 2081 Fragments arranged by size: 2151 2081 1626 47 StuI AGG'CCT Cuts at: 1517 2858 3161 1517 Size: 1341 303 4261 Fragments arranged by size: 4261 1341 303 StyI C'CwwG_G Cuts at: 513 1065 3344 513 Size: 552 2279 3074 Fragments arranged by size: 3074 2279 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2539 3932 3949 4102 4287 4732 325 Size: 1255 959 1393 17 153 185 445 1498 Fragments arranged by size: 1498 1393 1255 959 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3072 4151 5863 Size: 274 80 33 51 1443 1095 1079 1712 Cuts at: 5863 96 Size: 138 Fragments arranged by size: 1712 1443 1095 1079 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2074 2106 5865 5897 2074 Size: 32 3759 32 2082 Fragments arranged by size: 3759 2082 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3044 1316 Size: 1728 4177 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2722 2722 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 3230 1870 Size: 1360 4545 XmnI GAAnn'nnTTC Cuts at: 4034 4034 Size: 5905 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2620 3043 3713 276 Size: 53 83 186 936 1086 423 670 2468 Fragments arranged by size: 2468 1086 936 670 423 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI