Restriction map for
pFN21AE6375
(Linear) (Six-base) MAPSORT of: KIEE6375.seq Check: 2157 from: 1 to: 1200
KIEE6375.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AatII G_ACGT'C
Cuts at: 0 617 1040 1200
Size: 617 423 160
Fragments arranged by size:
617 423 160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 606 915 1072 1200
Size: 61 545 309 157 128
Fragments arranged by size:
545 309 157 128 61
AhdI GACnn_n'nnGTC
Cuts at: 0 1015 1200
Size: 1015 185
AlwNI CAG_nnn'CTG
Cuts at: 0 1108 1200
Size: 1108 92
AvaI C'yCGr_G
Cuts at: 0 330 1012 1200
Size: 330 682 188
Fragments arranged by size:
682 330 188
BanI G'GyrC_C
Cuts at: 0 231 546 1134 1200
Size: 231 315 588 66
Fragments arranged by size:
588 315 231 66
BanII G_rGCy'C
Cuts at: 0 215 1200
Size: 215 985
BbsI GAAGACnn'nnnn_
Cuts at: 0 609 1011 1023 1035 1200
Size: 609 402 12 12 165
Fragments arranged by size:
609 402 165 12 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1140 1174 1200
Size: 1140 34 26
Fragments arranged by size:
1140 34 26
BciVI GTATCCnnnnn_n'
Cuts at: 0 329 1200
Size: 329 871
BclI T'GATC_A
Cuts at: 0 164 437 650 776 1200
Size: 164 273 213 126 424
Fragments arranged by size:
424 273 213 164 126
Bme1580I G_kGCm'C
Cuts at: 0 551 1137 1200
Size: 551 586 63
Fragments arranged by size:
586 551 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 1140 1200
Size: 1140 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 260 909 937 1200
Size: 121 139 649 28 263
Fragments arranged by size:
649 263 139 121 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1200
Size: 229 971
BsaI GGTCTCn'nnnn_
Cuts at: 0 986 1071 1200
Size: 986 85 129
Fragments arranged by size:
986 129 85
BsaAI yAC'GTr
Cuts at: 0 782 1200
Size: 782 418
BsaBI GATnn'nnATC
Cuts at: 0 655 1200
Size: 655 545
BsaHI Gr'CG_yC
Cuts at: 0 614 1037 1200
Size: 614 423 163
Fragments arranged by size:
614 423 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 69 99 1200
Size: 69 30 1101
Fragments arranged by size:
1101 69 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 243 529 562 1200
Size: 243 286 33 638
Fragments arranged by size:
638 286 243 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 682 1200
Size: 613 69 518
Fragments arranged by size:
613 518 69
BsiHKAI G_wGCw'C
Cuts at: 0 215 728 908 1200
Size: 215 513 180 292
Fragments arranged by size:
513 292 215 180
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1123 1200
Size: 1123 77
Bsp1286I G_dGCh'C
Cuts at: 0 215 551 728 908 1137 1200
Size: 215 336 177 180 229 63
Fragments arranged by size:
336 229 215 180 177 63
BspHI T'CATG_A
Cuts at: 0 440 1200
Size: 440 760
BsrFI r'CCGG_y
Cuts at: 0 325 1200
Size: 325 875
BsrGI T'GTAC_A
Cuts at: 0 1067 1200
Size: 1067 133
BssHII G'CGCG_C
Cuts at: 0 1096 1200
Size: 1096 104
BstXI CCAn_nnnn'nTGG
Cuts at: 0 388 759 1200
Size: 388 371 441
Fragments arranged by size:
441 388 371
BstYI r'GATC_y
Cuts at: 0 395 1200
Size: 395 805
Bsu36I CC'TnA_GG
Cuts at: 0 431 1200
Size: 431 769
BtsI GCAGTG_nn'
Cuts at: 0 670 773 1200
Size: 670 103 427
Fragments arranged by size:
670 427 103
EaeI y'GGCC_r
Cuts at: 0 139 687 1200
Size: 139 548 513
Fragments arranged by size:
548 513 139
EarI CTCTTCn'nnn_
Cuts at: 0 742 802 1200
Size: 742 60 398
Fragments arranged by size:
742 398 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 468 1200
Size: 468 732
EcoICRI GAG'CTC
Cuts at: 0 213 1200
Size: 213 987
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 121 260 606 909 915 937 1072
Size: 61 60 139 346 303 6 22 135
Cuts at: 1072 1200
Size: 128
Fragments arranged by size:
346 303 139 135 128 61 60 22
6
HaeII r_GCGC'y
Cuts at: 0 365 1200
Size: 365 835
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1001 1033 1200
Size: 1001 32 167
Fragments arranged by size:
1001 167 32
HindIII A'AGCT_T
Cuts at: 0 1140 1200
Size: 1140 60
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 1200
Size: 261 939
MscI TGG'CCA
Cuts at: 0 689 1200
Size: 689 511
MslI CAynn'nnrTG
Cuts at: 0 658 757 1200
Size: 658 99 443
Fragments arranged by size:
658 443 99
PfoI T'CCnGG_A
Cuts at: 0 237 908 1200
Size: 237 671 292
Fragments arranged by size:
671 292 237
PmlI CAC'GTG
Cuts at: 0 782 1200
Size: 782 418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 1200
Size: 97 32 1071
Fragments arranged by size:
1071 97 32
PshAI GACnn'nnGTC
Cuts at: 0 121 1200
Size: 121 1079
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 853 885 1200
Size: 853 32 315
Fragments arranged by size:
853 315 32
PstI C_TGCA'G
Cuts at: 0 768 791 944 1200
Size: 768 23 153 256
Fragments arranged by size:
768 256 153 23
SacI G_AGCT'C
Cuts at: 0 215 1200
Size: 215 985
SapI GCTCTTCn'nnn_
Cuts at: 0 742 1200
Size: 742 458
SexAI A'CCwGG_T
Cuts at: 0 380 1200
Size: 380 820
SfcI C'TryA_G
Cuts at: 0 516 764 787 940 1200
Size: 516 248 23 153 260
Fragments arranged by size:
516 260 248 153 23
SmlI C'TyrA_G
Cuts at: 0 208 1200
Size: 208 992
SspI AAT'ATT
Cuts at: 0 198 1200
Size: 198 1002
StuI AGG'CCT
Cuts at: 0 853 1156 1200
Size: 853 303 44
Fragments arranged by size:
853 303 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 534 1200
Size: 534 666
TatI w'GTAC_w
Cuts at: 0 1067 1200
Size: 1067 133
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 69 101 1200
Size: 69 32 1099
Fragments arranged by size:
1099 69 32
Tth111I GACn'n_nGTC
Cuts at: 0 1039 1200
Size: 1039 161
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 717 1200
Size: 717 483
ZraI GAC'GTC
Cuts at: 0 615 1038 1200
Size: 615 423 162
Fragments arranged by size:
615 423 162
Enzymes that do cut:
AatII AcuI AhdI AlwNI AvaI BanI BanII BbsI
BcgI BciVI BclI Bme1580I BmrI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BsrFI BsrGI BssHII BstXI BstYI Bsu36I
BtsI EaeI EarI EciI EcoICRI Eco57MI HaeII Hin4I
HindIII MmeI MscI MslI PfoI PmlI PpiI PshAI
PsrI PstI SacI SapI SexAI SfcI SmlI SspI
StuI TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BfrBI BglI
BglII BlpI BmgBI BmtI BplI Bpu10I BsaWI BseYI
BsiEI BsiWI BsmI BspEI BspMI BsrBI BsrDI BssSI
BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EagI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PpuMI PsiI PspOMI PspXI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StyI
SwaI XbaI XhoI XmaI XmnI