################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HapII HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MflI MluI MroI MseI MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 310 GACGCC G[GA]^CG[CT]C AfaI 204 GTAC GT^AC AflIII 745 ACATGT A^C[GA][CT]GT AlwNI 26 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 732 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 112 GTGCTC G[AT]GC[AT]^C BanI 651 GGCACC G^G[CT][GA]CC BanII 181 GAGCCC G[GA]GC[CT]^C BclI 866 TGATCA T^GATCA BfaI 76 CTAG C^TAG BglI 637 GCCTTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 594 CTCCAG CTGGAG(16/14) BsaHI 310 GACGCC G[GA]^CG[CT]C BsiHKAI 112 GTGCTC G[AT]GC[AT]^C BsmAI 278 GTCTC GTCTC(1/5) BsmBI 277 CGTCTC CGTCTC(1/5) BspHI 857 TCATGA T^CATGA BspLU11I 745 ACATGT A^CATGT BsrBI 711 CCGCTC CCGCTC(-3/-3) BsrDI 265 GCAATG GCAATG(2/0) CfoI 923 GCGC GCG^C DraIII 502 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 377 CCATGG C^C[GA][CT]GG FauI 428 CCCGC CCCGC(4/6) FbaI 866 TGATCA T^GATCA HaeII 922 GGCGCT [GA]GCGC^[CT] HhaI 923 GCGC GCG^C Hin1I 310 GACGCC G[GA]^CG[CT]C HinP1I 923 GCGC G^CGC MaeI 76 CTAG C^TAG MfeI 140 CAATTG C^AATTG MspA1I 47 CCGCTG C[AC]G^C[GT]G MunI 140 CAATTG C^AATTG NcoI 377 CCATGG C^CATGG NspI 745 ACATGT [GA]CATG^[CT] PshAI 786 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 857 TCATGA T^CATGA RsaI 204 GTAC GT^AC StuI 734 AGGCCT AGG^CCT TfiI 823 GAATC G^A[AT]TC XcmI 339 CCACCTTCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 732 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AvaII 35 GGACC G^G[AT]CC 44 GGACC BalI 398 TGGCCA TGG^CCA 509 TGGCCA BbsI 661 GTCTTC GAAGAC(2/6) 793 GTCTTC BbvCI 153 CCTCAGC CCTCAGC(-5/-2) 718 GCTGAGG BmyI 112 GTGCTC G[AGT]GC[ACT]^C 181 GAGCCC BpuAI 661 GTCTTC GAAGAC(2/6) 793 GTCTTC BseRI 115 CTCCTC GAGGAG(10/8) 118 CTCCTC Bsp1286I 112 GTGCTC G[AGT]GC[ACT]^C 181 GAGCCC BsrI 69 ACTGG ACTGG(1/-1) 100 CCAGT Cac8I 313 GCCTGC GC[ACGT]^[ACGT]GC 407 GCCAGC DpnII 867 GATC ^GATC 953 GATC EaeI 398 TGGCCA [CT]^GGCC[GA] 509 TGGCCA EcoT14I 377 CCATGG C^C[AT][AT]GG 656 CCTTGG HgaI 310 GACGC GACGC(5/10) 466 GCGTC HphI 416 TCACC GGTGA(8/7) 485 TCACC MboI 867 GATC ^GATC 953 GATC MluNI 398 TGGCCA TGG^CCA 509 TGGCCA MscI 398 TGGCCA TGG^CCA 509 TGGCCA NdeII 867 GATC ^GATC 953 GATC PleI 392 GACTC GAGTC(4/5) 435 GAGTC SapI 327 GCTCTTC GCTCTTC(1/4) 576 GCTCTTC Sau3AI 867 GATC ^GATC 953 GATC SfcI 420 CTACAG C^T[GA][CT]AG 894 CTACAG StyI 377 CCATGG C^C[AT][AT]GG 656 CCTTGG Tsp509I 141 AATT ^AATT 300 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI Cfr13I DdeI EarI EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeIII MboII MnlI MslI MvaI MwoI NlaIII NlaIV PflMI Sau96I ScrFI SfaNI TseI Tsp45I TspRI Van91I