(Circular) (Six-base) MAPSORT of: pFN21AE6067.seq Check: 8844 from: 1 to: 5668 pFN21AE6067 5668 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2989 2989 Size: 5668 AclI AA'CG_TT Cuts at: 1563 3795 4168 1563 Size: 2232 373 3063 Fragments arranged by size: 3063 2232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2785 2817 3731 5115 5383 1632 Size: 1153 32 914 1384 268 1917 Fragments arranged by size: 1917 1384 1153 914 268 32 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5471 AflIII A'CryG_T Cuts at: 1203 2750 4567 5544 1203 Size: 1547 1817 977 1327 Fragments arranged by size: 1817 1547 1327 977 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 4397 4397 Size: 5668 AleI CACnn'nnGTG Cuts at: 1166 2771 1166 Size: 1605 4063 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5668 AlwNI CAG_nnn'CTG Cuts at: 2036 3411 4983 2036 Size: 1375 1572 2721 Fragments arranged by size: 2721 1572 1375 ApaLI G'TGCA_C Cuts at: 3725 4881 3725 Size: 1156 4512 ApoI r'AATT_y Cuts at: 1493 2977 3203 3355 5464 1493 Size: 1484 226 152 2109 1697 Fragments arranged by size: 2109 1697 1484 226 152 AseI AT'TA_AT Cuts at: 160 1032 4222 160 Size: 872 3190 1606 Fragments arranged by size: 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2993 1870 Size: 73 1050 4545 Fragments arranged by size: 4545 1050 73 BamHI G'GATC_C Cuts at: 2998 3365 2998 Size: 367 5301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2656 2989 4444 618 Size: 325 217 627 869 333 1455 1842 Fragments arranged by size: 1842 1455 869 627 333 325 217 BanII G_rGCy'C Cuts at: 1391 2190 2987 1391 Size: 799 797 4072 Fragments arranged by size: 4072 799 797 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2659 2791 928 Size: 971 760 132 3805 Fragments arranged by size: 3805 971 760 132 BbvCI CC'TCA_GC Cuts at: 2159 2724 2159 Size: 565 5103 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3859 3893 1903 Size: 34 1922 34 3678 Fragments arranged by size: 3678 1922 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 2871 1548 Size: 1323 4345 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2648 4279 136 Size: 107 122 71 631 605 976 1631 1525 Fragments arranged by size: 1631 1525 976 631 605 122 107 71 BglII A'GATC_T Cuts at: 5663 5663 Size: 5668 BlpI GC'TnA_GC Cuts at: 3085 3085 Size: 5668 Bme1580I G_kGCm'C Cuts at: 1790 3729 4885 1790 Size: 1939 1156 2573 Fragments arranged by size: 2573 1939 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5668 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2084 3399 4357 Size: 416 53 83 399 24 667 1315 958 Cuts at: 4357 442 Size: 1753 Fragments arranged by size: 1753 1315 958 667 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2584 4328 1239 Size: 1345 1744 2579 Fragments arranged by size: 2579 1744 1345 Bpu10I CC'TnA_GC Cuts at: 2159 2724 2159 Size: 565 5103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3793 4658 4956 3793 Size: 865 298 4505 Fragments arranged by size: 4505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4331 882 Size: 617 2832 2219 Fragments arranged by size: 2832 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3459 493 Size: 711 2255 2702 Fragments arranged by size: 2702 2255 711 BsaBI GATnn'nnATC Cuts at: 5662 5662 Size: 5668 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2316 3475 3857 4492 Size: 53 83 186 936 783 1159 382 635 Cuts at: 4492 275 Size: 1451 Fragments arranged by size: 1451 1159 936 783 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4101 4499 4773 4920 5331 4101 Size: 398 274 147 411 4438 Fragments arranged by size: 4438 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5443 Fragments arranged by size: 5443 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2114 1186 Size: 925 3 4740 Fragments arranged by size: 4740 925 3 BseYI C'CCAG_C Cuts at: 2036 2328 2354 2815 4871 5367 2036 Size: 292 26 461 2056 496 2337 Fragments arranged by size: 2337 2056 496 461 292 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5095 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3118 3879 4907 1540 Size: 275 186 1117 761 1028 2301 Fragments arranged by size: 2301 1117 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2121 3729 3814 4885 2121 Size: 1608 85 1071 2904 Fragments arranged by size: 2904 1608 1071 85 BsmI GAATG_Cn' Cuts at: 3179 3272 3179 Size: 93 5575 BsmBI CGTCTCn'nnnn_ Cuts at: 2288 2288 Size: 5668 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2190 2987 3729 3814 4885 1391 Size: 399 331 69 797 742 85 1071 2174 Fragments arranged by size: 2174 1071 797 742 399 331 85 69 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 2862 3557 5287 2862 Size: 695 1730 3243 Fragments arranged by size: 3243 1730 695 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3023 844 Size: 2179 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2718 3555 1394 Size: 429 895 837 3507 Fragments arranged by size: 3507 895 837 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2277 4163 4337 65 Size: 1183 1029 1886 174 1396 Fragments arranged by size: 1886 1396 1183 1029 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4312 4499 1700 Size: 110 2502 187 2869 Fragments arranged by size: 2869 2502 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5668 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5668 BssSI C'ACGA_G Cuts at: 3728 4740 3728 Size: 1012 4656 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5668 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5668 BstYI r'GATC_y Cuts at: 1972 2998 3365 3750 3767 5219 5305 5317 5663 Size: 1026 367 385 17 1452 86 12 346 Cuts at: 5663 1972 Size: 1977 Fragments arranged by size: 1977 1452 1026 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2382 513 Size: 552 1317 3799 Fragments arranged by size: 3799 1317 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4929 Fragments arranged by size: 4929 683 56 BtsI GCAGTG_nn' Cuts at: 3184 3978 3998 3184 Size: 794 20 4854 Fragments arranged by size: 4854 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2518 2553 529 Size: 35 1954 35 3644 Fragments arranged by size: 3644 1954 35 35 DraI TTT'AAA Cuts at: 2972 2972 Size: 5668 DraIII CAC_nnn'GTG Cuts at: 2512 2512 Size: 5668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4675 809 Size: 507 3359 1802 Fragments arranged by size: 3359 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2403 2514 3115 Size: 54 1425 50 275 18 573 111 601 Cuts at: 3115 4004 5633 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 601 573 275 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3115 1537 Size: 275 1303 4090 Fragments arranged by size: 4090 1303 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2088 2339 2588 3598 1353 Size: 735 251 249 1010 3423 Fragments arranged by size: 3423 1010 735 251 249 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4255 4629 4775 387 Size: 1064 138 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2584 2785 2817 3731 4328 5115 5383 Size: 393 952 201 32 914 597 787 268 Cuts at: 5383 1239 Size: 1524 Fragments arranged by size: 1524 952 914 787 597 393 268 201 32 EcoRI G'AATT_C Cuts at: 2977 3355 2977 Size: 378 5290 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2758 2758 Size: 5668 FspI TGC'GCA Cuts at: 4174 4174 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 2931 4815 1678 Size: 1253 1884 2531 Fragments arranged by size: 2531 1884 1253 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4309 4341 4383 4415 1373 Size: 32 135 32 2737 32 42 32 2626 Fragments arranged by size: 2737 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3258 669 Size: 988 281 1074 246 3079 Fragments arranged by size: 3079 1074 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5668 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KpnI G_GTAC'C Cuts at: 2993 2993 Size: 5668 MfeI C'AATT_G Cuts at: 2145 3267 2145 Size: 1122 4546 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2224 4782 4966 1305 Size: 919 2558 184 2007 Fragments arranged by size: 2558 2007 919 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2405 2516 10 Size: 54 1425 343 573 111 3162 Fragments arranged by size: 3162 1425 573 343 111 54 MslI CAynn'nnrTG Cuts at: 518 1166 2435 2651 2675 2771 2867 3626 3985 Size: 648 1269 216 24 96 96 759 359 Cuts at: 3985 4144 518 Size: 159 2042 Fragments arranged by size: 2042 1269 759 648 359 216 159 96 96 24 MspA1I CmG'CkG Cuts at: 1606 2054 3084 3761 4909 5154 1606 Size: 448 1030 677 1148 245 2120 Fragments arranged by size: 2120 1148 1030 677 448 245 NcoI C'CATG_G Cuts at: 513 1065 2382 513 Size: 552 1317 3799 Fragments arranged by size: 3799 1317 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5668 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5668 NotI GC'GGCC_GC Cuts at: 3115 3115 Size: 5668 NspI r_CATG'y Cuts at: 1132 1229 2754 3026 4571 1132 Size: 97 1525 272 1545 2229 Fragments arranged by size: 2229 1545 1525 272 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5668 PciI A'CATG_T Cuts at: 2750 4567 2750 Size: 1817 3851 PflMI CCAn_nnn'nTGG Cuts at: 1108 2036 2350 2512 1108 Size: 928 314 162 4264 Fragments arranged by size: 4264 928 314 162 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2803 2835 3737 3769 5250 5282 2803 Size: 32 902 32 1481 32 3189 Fragments arranged by size: 3189 1481 902 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2795 1613 Size: 1182 4486 PsiI TTA'TAA Cuts at: 3238 3238 Size: 5668 PstI C_TGCA'G Cuts at: 830 3020 830 Size: 2190 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SapI GCTCTTCn'nnn_ Cuts at: 2339 2588 2339 Size: 249 5419 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 3916 5628 3916 Size: 1712 3956 SfcI C'TryA_G Cuts at: 826 1045 2425 2899 3016 4151 4832 5023 826 Size: 219 1380 474 117 1135 681 191 1471 Fragments arranged by size: 1471 1380 1135 681 474 219 191 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2995 1872 Size: 1123 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3772 4673 4935 820 Size: 197 926 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 3026 1132 Size: 1894 3774 SspI AAT'ATT Cuts at: 5 52 3592 5 Size: 47 3540 2081 Fragments arranged by size: 3540 2081 47 StuI AGG'CCT Cuts at: 1517 2741 1517 Size: 1224 4444 StyI C'CwwG_G Cuts at: 513 1065 2382 2661 3107 513 Size: 552 1317 279 446 3074 Fragments arranged by size: 3074 1317 552 446 279 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2409 2621 3695 3712 3865 4050 4495 Size: 1255 829 212 1074 17 153 185 445 Cuts at: 4495 325 Size: 1498 Fragments arranged by size: 1498 1255 1074 829 445 212 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2208 3914 5626 Size: 274 80 33 51 1443 231 1706 1712 Cuts at: 5626 96 Size: 138 Fragments arranged by size: 1712 1706 1443 274 231 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2568 2600 5628 5660 2568 Size: 32 3028 32 2576 Fragments arranged by size: 3028 2576 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5668 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2351 2351 Size: 5668 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5668 XmaI C'CCGG_G Cuts at: 1870 2993 1870 Size: 1123 4545 XmnI GAAnn'nnTTC Cuts at: 2741 3797 2741 Size: 1056 4612 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI