################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AclI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BfrI BlnI BpuAI BsaAI BsaBI BsgI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FseI FspI HincII HindII HpaI KpnI KspI MaeII MamI MfeI MluI MluNI MscI MunI NdeI NheI NotI NruI NsiI NspI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 505 AAATTT [GA]^AATT[CT] AcyI 1044 GGCGCC G[GA]^CG[CT]C AlwNI 252 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 505 AAATTT [GA]^AATT[CT] BbeI 1044 GGCGCC GGCGC^C BclI 927 TGATCA T^GATCA BglI 1174 GCCATGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 682 AGATCT A^GATCT BlpI 309 GCTGAGC GC^T[ACGT]AGC Bpu1102I 309 GCTGAGC GC^T[ACGT]AGC BsaHI 1044 GGCGCC G[GA]^CG[CT]C BseRI 229 GAGGAG GAGGAG(10/8) BsiEI 37 CGACCG CG[GA][CT]^CG BsmI 615 GCATTC GAATGC(1/-1) BsrBI 223 CCGCTC CCGCTC(-3/-3) BsrFI 1047 GCCGGC [GA]^CCGG[CT] BssHII 153 GCGCGC G^CGCGC BstAPI 615 GCATTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1198 TTCGAA TT^CGAA CelII 309 GCTGAGC GC^T[ACGT]AGC Cfr10I 1047 GCCGGC [GA]^CCGG[CT] DraII 65 GGGACCT [GA]G^G[ACGT]CC[CT] DrdI 841 GACACTCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 492 CCTCATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 65 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 514 GATATC GAT^ATC FbaI 927 TGATCA T^GATCA Hin1I 1044 GGCGCC G[GA]^CG[CT]C HindIII 287 AAGCTT A^AGCTT KasI 1044 GGCGCC G^GCGCC NaeI 1047 GCCGGC GCC^GGC NarI 1044 GGCGCC GG^CGCC NgoMIV 1047 GCCGGC G^CCGGC NspV 1198 TTCGAA TT^CGAA PaeR7I 226 CTCGAG C^TCGAG PflMI 251 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 65 GGGACCT [GA]G^G[AT]CC[CT] SfoI 1044 GGCGCC GGC^GCC SfuI 1198 TTCGAA TT^CGAA SmaI 295 CCCGGG CCC^GGG Van91I 251 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 444 TCTAGA T^CTAGA XcmI 722 CCAAAGGCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 226 CTCGAG C^TCGAG XmaCI 295 CCCGGG C^CCGGG XmaI 295 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccIII 130 TCCGGA T^CCGGA 782 TCCGGA AlwI 366 GATCC GGATC(4/5) 1137 GATCC AspHI 754 GTGCTC G[AT]GC[AT]^C 1113 GAGCAC BanI 180 GGCACC G^G[CT][GA]CC 1044 GGCGCC BanII 105 GAGCCC G[GA]GC[CT]^C 745 GAGCCC BfaI 445 CTAG C^TAG 905 CTAG BpmI 410 CTGGAG CTGGAG(16/14) 1090 CTGGAG BsaI 15 GAGACC GGTCTC(1/5) 1072 GAGACC BseAI 130 TCCGGA T^CCGGA 782 TCCGGA BsiHKAI 754 GTGCTC G[AT]GC[AT]^C 1113 GAGCAC BsmAI 15 GAGAC GTCTC(1/5) 1072 GAGAC BspEI 130 TCCGGA T^CCGGA 782 TCCGGA BspMI 830 ACCTGC ACCTGC(4/8) 48 GCAGGT BstYI 365 AGATCC [GA]^GATC[CT] 682 AGATCT DsaI 912 CCATGG C^C[GA][CT]GG 1175 CCATGG EaeI 1106 TGGCCG [CT]^GGCC[GA] 1172 CGGCCA EarI 262 GAAGAG CTCTTC(1/4) 961 GAAGAG EcoT14I 912 CCATGG C^C[AT][AT]GG 1175 CCATGG HgaI 73 GACGC GACGC(5/10) 397 GACGC Ksp632I 262 GAAGAG CTCTTC(1/4) 961 GAAGAG MaeI 445 CTAG C^TAG 905 CTAG MflI 365 AGATCC [GA]^GATC[CT] 682 AGATCT MroI 130 TCCGGA T^CCGGA 782 TCCGGA MseI 497 TTAA T^TAA 584 TTAA MslI 495 CATTAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 949 CACAAGTATG NcoI 912 CCATGG C^CATGG 1175 CCATGG StyI 912 CCATGG C^C[AT][AT]GG 1175 CCATGG Tru9I 497 TTAA T^TAA 584 TTAA XhoII 365 AGATCC [GA]^GATC[CT] 682 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AluI AvaI AvaII BbvI BcnI BmyI BsaJI BsaWI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I