(Circular) (Six-base) MAPSORT of: pFN21AE1891.seq Check: 6141 from: 1 to: 5965 pFN21AE1891 5965 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2044 2044 Size: 5965 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3775 278 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3308 1937 Size: 1371 4594 Acc65I G'GTAC_C Cuts at: 3286 3286 Size: 5965 AclI AA'CG_TT Cuts at: 1563 4092 4465 1563 Size: 2529 373 3063 Fragments arranged by size: 3063 2529 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2600 4028 5412 5680 1632 Size: 968 1428 1384 268 1917 Fragments arranged by size: 1917 1428 1384 968 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5768 AflIII A'CryG_T Cuts at: 1203 4864 5841 1203 Size: 3661 977 1327 Fragments arranged by size: 3661 1327 977 AgeI A'CCGG_T Cuts at: 4796 4796 Size: 5965 AhdI GACnn_n'nnGTC Cuts at: 4694 4694 Size: 5965 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5965 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3152 1184 Size: 1968 3997 AlwNI CAG_nnn'CTG Cuts at: 2262 3708 5280 2262 Size: 1446 1572 2947 Fragments arranged by size: 2947 1572 1446 ApaLI G'TGCA_C Cuts at: 4022 5178 4022 Size: 1156 4809 ApoI r'AATT_y Cuts at: 1493 2510 3274 3500 3652 5761 1493 Size: 1017 764 226 152 2109 1697 Fragments arranged by size: 2109 1697 1017 764 226 152 AseI AT'TA_AT Cuts at: 160 1032 4519 160 Size: 872 3487 1606 Fragments arranged by size: 3487 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 AvaI C'yCGr_G Cuts at: 1870 1943 2231 2300 2850 3290 1870 Size: 73 288 69 550 440 4545 Fragments arranged by size: 4545 550 440 288 73 69 BamHI G'GATC_C Cuts at: 3295 3662 3295 Size: 367 5598 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2185 3049 3286 4741 618 Size: 325 217 627 398 864 237 1455 1842 Fragments arranged by size: 1842 1455 864 627 398 325 237 217 BanII G_rGCy'C Cuts at: 1391 2114 2754 3284 1391 Size: 723 640 530 4072 Fragments arranged by size: 4072 723 640 530 BbeI G_GCGC'C Cuts at: 3053 3053 Size: 5965 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4994 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4156 4190 1903 Size: 34 2219 34 3678 Fragments arranged by size: 3678 2219 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3857 5067 3857 Size: 1210 4755 BclI T'GATC_A Cuts at: 1548 2932 1548 Size: 1384 4581 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3185 4576 Size: 107 122 71 631 605 337 1176 1391 Cuts at: 4576 136 Size: 1525 Fragments arranged by size: 1525 1391 1176 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2687 5960 2687 Size: 3273 2692 BlpI GC'TnA_GC Cuts at: 2315 3382 2315 Size: 1067 4898 Bme1580I G_kGCm'C Cuts at: 1790 2206 2829 4026 5182 1790 Size: 416 623 1197 1156 2573 Fragments arranged by size: 2573 1197 1156 623 416 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5965 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2108 3696 4654 Size: 416 53 83 399 24 691 1588 958 Cuts at: 4654 442 Size: 1753 Fragments arranged by size: 1753 1588 958 691 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5965 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2436 3116 4625 1239 Size: 1197 680 1509 2579 Fragments arranged by size: 2579 1509 1197 680 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2393 3262 4090 4955 5253 2393 Size: 869 828 865 298 3105 Fragments arranged by size: 3105 869 865 828 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2014 3071 4628 882 Size: 617 515 1057 1557 2219 Fragments arranged by size: 2219 1557 1057 617 515 BsaAI yAC'GTr Cuts at: 493 1204 3756 493 Size: 711 2552 2702 Fragments arranged by size: 2702 2552 711 BsaBI GATnn'nnATC Cuts at: 5959 5959 Size: 5965 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3050 3772 4154 4789 Size: 53 83 186 936 1517 722 382 635 Cuts at: 4789 275 Size: 1451 Fragments arranged by size: 1517 1451 936 722 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2135 2787 2984 4398 4796 5070 5217 5628 2135 Size: 652 197 1414 398 274 147 411 2472 Fragments arranged by size: 2472 1414 652 411 398 274 197 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2130 2160 1178 Size: 30 165 30 727 30 4983 Fragments arranged by size: 4983 727 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 1186 Size: 1063 4902 BseYI C'CCAG_C Cuts at: 2088 2255 2696 2922 5168 5664 2088 Size: 167 441 226 2246 496 2389 Fragments arranged by size: 2389 2246 496 441 226 167 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5392 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2045 3415 4176 5204 1540 Size: 275 186 44 1370 761 1028 2301 Fragments arranged by size: 2301 1370 1028 761 275 186 44 BsiHKAI G_wGCw'C Cuts at: 2763 3122 4026 4111 5182 2763 Size: 359 904 85 1071 3546 Fragments arranged by size: 3546 1071 904 359 85 BsmI GAATG_Cn' Cuts at: 2620 3476 3569 2620 Size: 856 93 5016 Fragments arranged by size: 5016 856 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2114 2206 2754 2763 2829 3122 3284 Size: 399 324 92 548 9 66 293 162 Cuts at: 3284 4026 4111 5182 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 548 399 324 293 162 92 85 66 9 BspEI T'CCGG_A Cuts at: 2135 2787 5628 2135 Size: 652 2841 2472 Fragments arranged by size: 2841 2472 652 BspHI T'CATG_A Cuts at: 3854 5584 3854 Size: 1730 4235 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2044 2844 3320 844 Size: 1200 800 476 3489 Fragments arranged by size: 3489 1200 800 476 BsrBI CCG'CTC Cuts at: 1394 1823 2230 3852 1394 Size: 429 407 1622 3507 Fragments arranged by size: 3507 1622 429 407 BsrDI GCAATG_nn' Cuts at: 65 1248 4460 4634 65 Size: 1183 3212 174 1396 Fragments arranged by size: 3212 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3052 4609 4796 1700 Size: 110 1242 1557 187 2869 Fragments arranged by size: 2869 1557 1242 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5965 BssHII G'CGCG_C Cuts at: 1925 2158 1925 Size: 233 5732 BssSI C'ACGA_G Cuts at: 4025 5037 4025 Size: 1012 4953 BstAPI GCAn_nnn'nTGC Cuts at: 794 2626 794 Size: 1832 4133 BstBI TT'CG_AA Cuts at: 3204 4769 3204 Size: 1565 4400 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5965 BstYI r'GATC_y Cuts at: 1972 2370 2687 3295 3662 4047 4064 5516 5602 Size: 398 317 608 367 385 17 1452 86 Cuts at: 5602 5614 5960 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 608 398 385 367 346 317 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2917 3180 513 Size: 552 939 913 263 3298 Fragments arranged by size: 3298 939 913 552 263 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5226 Fragments arranged by size: 5226 683 56 BtsI GCAGTG_nn' Cuts at: 2326 2624 3481 4275 4295 2326 Size: 298 857 794 20 3996 Fragments arranged by size: 3996 857 794 298 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5965 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5930 DraI TTT'AAA Cuts at: 3269 3269 Size: 5965 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2852 4972 809 Size: 507 1536 2120 1802 Fragments arranged by size: 2120 1802 1536 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3111 3177 3412 Size: 54 1425 50 275 18 1281 66 235 Cuts at: 3412 4301 5930 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1281 889 275 235 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3412 1537 Size: 275 1600 4090 Fragments arranged by size: 4090 1600 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2262 2961 3895 1353 Size: 909 699 934 3423 Fragments arranged by size: 3423 934 909 699 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2122 3248 4552 4926 5072 387 Size: 1064 138 533 1126 1304 374 146 1280 Fragments arranged by size: 1304 1280 1126 1064 533 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2436 2600 3116 4028 4625 5412 5680 Size: 393 804 164 516 912 597 787 268 Cuts at: 5680 1239 Size: 1524 Fragments arranged by size: 1524 912 804 787 597 516 393 268 164 EcoNI CCTnn'n_nnAGG Cuts at: 2501 2501 Size: 5965 EcoO109I rG'GnC_Cy Cuts at: 2071 2071 Size: 5965 EcoRI G'AATT_C Cuts at: 3274 3652 3274 Size: 378 5587 EcoRV GAT'ATC Cuts at: 2521 2521 Size: 5965 FspI TGC'GCA Cuts at: 4471 4471 Size: 5965 HaeII r_GCGC'y Cuts at: 1678 2015 2089 2102 3053 5112 1678 Size: 337 74 13 951 2059 2531 Fragments arranged by size: 2531 2059 951 337 74 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2228 2260 2584 2616 4606 Size: 32 135 32 656 32 324 32 1990 Cuts at: 4606 4638 4680 4712 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1990 656 324 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3309 3555 669 Size: 988 281 1371 246 3079 Fragments arranged by size: 3079 1371 988 281 246 HindIII A'AGCT_T Cuts at: 748 2292 748 Size: 1544 4421 HpaI GTT'AAC Cuts at: 3555 3555 Size: 5965 KasI G'GCGC_C Cuts at: 3049 3049 Size: 5965 KpnI G_GTAC'C Cuts at: 3290 3290 Size: 5965 MfeI C'AATT_G Cuts at: 3564 3564 Size: 5965 MluI A'CGCG_T Cuts at: 5841 5841 Size: 5965 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2559 5079 5263 1305 Size: 1254 2520 184 2007 Fragments arranged by size: 2520 2007 1254 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4143 Fragments arranged by size: 4143 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2504 2958 3923 4282 4441 518 Size: 648 1338 454 965 359 159 2042 Fragments arranged by size: 2042 1338 965 648 454 359 159 MspA1I CmG'CkG Cuts at: 1606 2696 3059 3109 3220 3381 4058 5206 5451 Size: 1090 363 50 111 161 677 1148 245 Cuts at: 5451 1606 Size: 2120 Fragments arranged by size: 2120 1148 1090 677 363 245 161 111 50 NaeI GCC'GGC Cuts at: 3054 3054 Size: 5965 NarI GG'CG_CC Cuts at: 3050 3050 Size: 5965 NcoI C'CATG_G Cuts at: 513 1065 2004 2917 3180 513 Size: 552 939 913 263 3298 Fragments arranged by size: 3298 939 913 552 263 NdeI CA'TA_TG Cuts at: 387 387 Size: 5965 NgoMIV G'CCGG_C Cuts at: 3052 3052 Size: 5965 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5965 NotI GC'GGCC_GC Cuts at: 3412 3412 Size: 5965 NspI r_CATG'y Cuts at: 1132 1229 3323 4868 1132 Size: 97 2094 1545 2229 Fragments arranged by size: 2229 2094 1545 97 PasI CC'CwG_GG Cuts at: 1348 2663 1348 Size: 1315 4650 PciI A'CATG_T Cuts at: 4864 4864 Size: 5965 PflMI CCAn_nnn'nTGG Cuts at: 1108 2262 1108 Size: 1154 4811 PmeI GTTT'AAAC Cuts at: 3269 3269 Size: 5965 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4034 4066 5547 5579 4034 Size: 32 1481 32 4420 Fragments arranged by size: 4420 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2071 2071 Size: 5965 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5965 PsiI TTA'TAA Cuts at: 3535 3535 Size: 5965 PspXI vC'TCGA_Gb Cuts at: 2231 2231 Size: 5965 PstI C_TGCA'G Cuts at: 830 2055 2695 2711 3108 3317 830 Size: 1225 640 16 397 209 3478 Fragments arranged by size: 3478 1225 640 397 209 16 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5965 PvuII CAG'CTG Cuts at: 2696 3059 3109 3220 2696 Size: 363 50 111 5441 Fragments arranged by size: 5441 363 111 50 SalI G'TCGA_C Cuts at: 1936 3307 1936 Size: 1371 4594 SbfI CC_TGCA'GG Cuts at: 3317 3317 Size: 5965 ScaI AGT'ACT Cuts at: 4213 5925 4213 Size: 1712 4253 SfcI C'TryA_G Cuts at: 826 1045 2051 2691 2707 3104 3313 4448 5129 Size: 219 1006 640 16 397 209 1135 681 Cuts at: 5129 5320 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1006 681 640 397 219 209 191 16 SfoI GGC'GCC Cuts at: 3051 3051 Size: 5965 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5965 SmaI CCC'GGG Cuts at: 1872 2302 3292 1872 Size: 430 990 4545 Fragments arranged by size: 4545 990 430 SmlI C'TyrA_G Cuts at: 820 1017 1943 2231 2408 3241 4069 4970 5232 Size: 197 926 288 177 833 828 901 262 Cuts at: 5232 820 Size: 1553 Fragments arranged by size: 1553 926 901 833 828 288 262 197 177 SnaBI TAC'GTA Cuts at: 493 3756 493 Size: 3263 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5965 SphI G_CATG'C Cuts at: 1132 3323 1132 Size: 2191 3774 SspI AAT'ATT Cuts at: 5 52 3889 5 Size: 47 3837 2081 Fragments arranged by size: 3837 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5965 StyI C'CwwG_G Cuts at: 513 1065 2004 2917 3180 3404 513 Size: 552 939 913 263 224 3074 Fragments arranged by size: 3074 939 913 552 263 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2914 3992 4009 4162 4347 4792 325 Size: 1255 1334 1078 17 153 185 445 1498 Fragments arranged by size: 1498 1334 1255 1078 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4211 5923 96 Size: 274 80 33 51 1443 2234 1712 138 Fragments arranged by size: 2234 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2130 2162 5925 5957 2130 Size: 32 3763 32 2138 Fragments arranged by size: 3763 2138 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5965 XbaI T'CTAG_A Cuts at: 2449 3301 2449 Size: 852 5113 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2734 2011 Size: 723 5242 XhoI C'TCGA_G Cuts at: 1943 2231 1943 Size: 288 5677 XmaI C'CCGG_G Cuts at: 1870 2300 3290 1870 Size: 430 990 4545 Fragments arranged by size: 4545 990 430 XmnI GAAnn'nnTTC Cuts at: 4094 4094 Size: 5965 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3773 276 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI