(Linear) (Six-base) MAPSORT of: KIEE1891.seq Check: 6688 from: 1 to: 1260 KIEE1891.orf With 182 enzymes: SgfI * July 15, 2010 18:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 39 1260 Size: 39 1221 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 595 1260 Size: 595 665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1147 1260 Size: 1147 113 AlwNI CAG_nnn'CTG Cuts at: 0 257 1260 Size: 257 1003 ApoI r'AATT_y Cuts at: 0 505 1260 Size: 505 755 AvaI C'yCGr_G Cuts at: 0 226 295 845 1260 Size: 226 69 550 415 Fragments arranged by size: 550 415 226 69 BanI G'GyrC_C Cuts at: 0 180 1044 1260 Size: 180 864 216 Fragments arranged by size: 864 216 180 BanII G_rGCy'C Cuts at: 0 109 749 1260 Size: 109 640 511 Fragments arranged by size: 640 511 109 BbeI G_GCGC'C Cuts at: 0 1048 1260 Size: 1048 212 BclI T'GATC_A Cuts at: 0 927 1260 Size: 927 333 BglI GCCn_nnn'nGGC Cuts at: 0 1180 1260 Size: 1180 80 BglII A'GATC_T Cuts at: 0 682 1260 Size: 682 578 BlpI GC'TnA_GC Cuts at: 0 310 1260 Size: 310 950 Bme1580I G_kGCm'C Cuts at: 0 201 824 1260 Size: 201 623 436 Fragments arranged by size: 623 436 201 BmrI ACTGGGnnnn_n' Cuts at: 0 103 1260 Size: 103 1157 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 431 1111 1260 Size: 431 680 149 Fragments arranged by size: 680 431 149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 1257 1260 Size: 388 869 3 Fragments arranged by size: 869 388 3 BsaI GGTCTCn'nnnn_ Cuts at: 0 9 1066 1260 Size: 9 1057 194 Fragments arranged by size: 1057 194 9 BsaHI Gr'CG_yC Cuts at: 0 1045 1260 Size: 1045 215 BsaWI w'CCGG_w Cuts at: 0 130 782 979 1260 Size: 130 652 197 281 Fragments arranged by size: 652 281 197 130 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 125 155 1260 Size: 125 30 1105 Fragments arranged by size: 1105 125 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 244 1260 Size: 244 1016 BseYI C'CCAG_C Cuts at: 0 83 250 691 917 1260 Size: 83 167 441 226 343 Fragments arranged by size: 441 343 226 167 83 BsiEI CG_ry'CG Cuts at: 0 40 1260 Size: 40 1220 BsiHKAI G_wGCw'C Cuts at: 0 758 1117 1260 Size: 758 359 143 Fragments arranged by size: 758 359 143 BsmI GAATG_Cn' Cuts at: 0 615 1260 Size: 615 645 Bsp1286I G_dGCh'C Cuts at: 0 109 201 749 758 824 1117 1260 Size: 109 92 548 9 66 293 143 Fragments arranged by size: 548 293 143 109 92 66 9 BspEI T'CCGG_A Cuts at: 0 130 782 1260 Size: 130 652 478 Fragments arranged by size: 652 478 130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 39 839 1260 Size: 39 800 421 Fragments arranged by size: 800 421 39 BsrBI CCG'CTC Cuts at: 0 225 1260 Size: 225 1035 BsrFI r'CCGG_y Cuts at: 0 1047 1260 Size: 1047 213 BssHII G'CGCG_C Cuts at: 0 153 1260 Size: 153 1107 BstAPI GCAn_nnn'nTGC Cuts at: 0 621 1260 Size: 621 639 BstBI TT'CG_AA Cuts at: 0 1199 1260 Size: 1199 61 BstYI r'GATC_y Cuts at: 0 365 682 1260 Size: 365 317 578 Fragments arranged by size: 578 365 317 BtgI C'CryG_G Cuts at: 0 912 1175 1260 Size: 912 263 85 Fragments arranged by size: 912 263 85 BtsI GCAGTG_nn' Cuts at: 0 321 619 1260 Size: 321 298 641 Fragments arranged by size: 641 321 298 DrdI GACnn_nn'nnGTC Cuts at: 0 847 1260 Size: 847 413 EaeI y'GGCC_r Cuts at: 0 1106 1172 1260 Size: 1106 66 88 Fragments arranged by size: 1106 88 66 EarI CTCTTCn'nnn_ Cuts at: 0 257 956 1260 Size: 257 699 304 Fragments arranged by size: 699 304 257 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 117 1243 1260 Size: 117 1126 17 Fragments arranged by size: 1126 117 17 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 431 595 1111 1260 Size: 431 164 516 149 Fragments arranged by size: 516 431 164 149 EcoNI CCTnn'n_nnAGG Cuts at: 0 496 1260 Size: 496 764 EcoO109I rG'GnC_Cy Cuts at: 0 66 1260 Size: 66 1194 EcoRV GAT'ATC Cuts at: 0 516 1260 Size: 516 744 HaeII r_GCGC'y Cuts at: 0 10 84 97 1048 1260 Size: 10 74 13 951 212 Fragments arranged by size: 951 212 74 13 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 223 255 579 611 1260 Size: 223 32 324 32 649 Fragments arranged by size: 649 324 223 32 32 HindIII A'AGCT_T Cuts at: 0 287 1260 Size: 287 973 KasI G'GCGC_C Cuts at: 0 1044 1260 Size: 1044 216 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 554 1260 Size: 554 706 MslI CAynn'nnrTG Cuts at: 0 499 953 1260 Size: 499 454 307 Fragments arranged by size: 499 454 307 MspA1I CmG'CkG Cuts at: 0 691 1054 1104 1215 1260 Size: 691 363 50 111 45 Fragments arranged by size: 691 363 111 50 45 NaeI GCC'GGC Cuts at: 0 1049 1260 Size: 1049 211 NarI GG'CG_CC Cuts at: 0 1045 1260 Size: 1045 215 NcoI C'CATG_G Cuts at: 0 912 1175 1260 Size: 912 263 85 Fragments arranged by size: 912 263 85 NgoMIV G'CCGG_C Cuts at: 0 1047 1260 Size: 1047 213 PasI CC'CwG_GG Cuts at: 0 658 1260 Size: 658 602 PflMI CCAn_nnn'nTGG Cuts at: 0 257 1260 Size: 257 1003 PpuMI rG'GwC_Cy Cuts at: 0 66 1260 Size: 66 1194 PspXI vC'TCGA_Gb Cuts at: 0 226 1260 Size: 226 1034 PstI C_TGCA'G Cuts at: 0 50 690 706 1103 1260 Size: 50 640 16 397 157 Fragments arranged by size: 640 397 157 50 16 PvuII CAG'CTG Cuts at: 0 691 1054 1104 1215 1260 Size: 691 363 50 111 45 Fragments arranged by size: 691 363 111 50 45 SfcI C'TryA_G Cuts at: 0 46 686 702 1099 1260 Size: 46 640 16 397 161 Fragments arranged by size: 640 397 161 46 16 SfoI GGC'GCC Cuts at: 0 1046 1260 Size: 1046 214 SmaI CCC'GGG Cuts at: 0 297 1260 Size: 297 963 SmlI C'TyrA_G Cuts at: 0 226 403 1236 1260 Size: 226 177 833 24 Fragments arranged by size: 833 226 177 24 StyI C'CwwG_G Cuts at: 0 912 1175 1260 Size: 912 263 85 Fragments arranged by size: 912 263 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 909 1260 Size: 909 351 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 125 157 1260 Size: 125 32 1103 Fragments arranged by size: 1103 125 32 XbaI T'CTAG_A Cuts at: 0 444 1260 Size: 444 816 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 729 1260 Size: 729 531 XhoI C'TCGA_G Cuts at: 0 226 1260 Size: 226 1034 XmaI C'CCGG_G Cuts at: 0 295 1260 Size: 295 965 Enzymes that do cut: AarI AcuI AlfI AlwNI ApoI AvaI BanI BanII BbeI BclI BglI BglII BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstAPI BstBI BstYI BtgI BtsI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HindIII KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PpuMI PspXI PstI PvuII SfcI SfoI SmaI SmlI StyI TaqII TstI XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsgI BsiWI BsmBI BspHI BsrDI BsrGI BssSI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EcoICRI EcoRI FalI FseI FspI FspAI HincII HpaI KpnI MfeI MluI MscI NdeI NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XmnI ZraI