Restriction map for
pFN21AE9288
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BamHI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsmI BspDI BspEI BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRV EcoT22I
FseI HpaI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tru9I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
AatII 862 GACGTC GACGT^C
AcsI 998 GAATTC [GA]^AATT[CT]
AflIII 1490 ACATGT A^C[GA][CT]GT
Alw44I 1501 GTGCAC G^TGCAC
AlwI 333 GGATC GGATC(4/5)
ApaLI 1501 GTGCAC G^TGCAC
ApoI 998 GAATTC [GA]^AATT[CT]
AspI 429 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 36 CTCGAG C^[CT]CG[GA]G
AviII 47 TGCGCA TGC^GCA
BalI 556 TGGCCA TGG^CCA
BbrPI 1228 CACGTG CAC^GTG
BbsI 906 GTCTTC GAAGAC(2/6)
BbvCI 748 CCTCAGC CCTCAGC(-5/-2)
BcgI 38 CGAGAAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 501 GGATAC GTATCC(6/5)
BclI 338 TGATCA T^GATCA
BpuAI 906 GTCTTC GAAGAC(2/6)
BsaAI 1228 CACGTG [CT]AC^GT[GA]
BsaI 667 GAGACC GGTCTC(1/5)
BsiWI 114 CGTACG C^GTACG
BsoBI 36 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1485 TGTACA T^GTACA
BspHI 1202 TCATGA T^CATGA
BspLU11I 1490 ACATGT A^CATGT
BspMI 1217 ACCTGC ACCTGC(4/8)
BsrBI 1165 GAGCGG CCGCTC(-3/-3)
BsrFI 1454 GCCGGC [GA]^CCGG[CT]
BsrGI 1485 TGTACA T^GTACA
BssSI 1150 CACGAG CACGAG(-5/-1)
BstAPI 182 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 333 GGATCT [GA]^GATC[CT]
Cfr10I 1454 GCCGGC [GA]^CCGG[CT]
EagI 1100 CGGCCG C^GGCCG
EarI 957 GAAGAG CTCTTC(1/4)
EclXI 1100 CGGCCG C^GGCCG
Eco52I 1100 CGGCCG C^GGCCG
EcoNI 849 CCTTTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 998 GAATTC G^AATTC
FbaI 338 TGATCA T^GATCA
FspI 47 TGCGCA TGC^GCA
HincII 244 GTCAAC GT[CT]^[GA]AC
HindII 244 GTCAAC GT[CT]^[GA]AC
HindIII 1039 AAGCTT A^AGCTT
HinfI 916 GAGTC G^A[ACGT]TC
Ksp632I 957 GAAGAG CTCTTC(1/4)
MflI 333 GGATCT [GA]^GATC[CT]
MluNI 556 TGGCCA TGG^CCA
MscI 556 TGGCCA TGG^CCA
NaeI 1454 GCCGGC GCC^GGC
NgoMIV 1454 GCCGGC G^CCGGC
PaeR7I 36 CTCGAG C^TCGAG
PflFI 429 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1552 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 916 GAGTC GAGTC(4/5)
PmaCI 1228 CACGTG CAC^GTG
PmlI 1228 CACGTG CAC^GTG
RcaI 1202 TCATGA T^CATGA
ScaI 443 AGTACT AGT^ACT
SspBI 1485 TGTACA T^GTACA
StuI 521 AGGCCT AGG^CCT
Tsp509I 999 AATT ^AATT
Tth111I 429 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1552 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 36 CTCGAG C^TCGAG
XhoII 333 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 106 CGCG CG^CG
720 CGCG
AlwNI 127 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
355 CAGTGCCTG
BbeI 66 GGCGCC GGCGC^C
576 GGCGCC
BglI 250 GCCTCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
359 GCCTGCACGGC
BsiEI 1100 CGGCCG CG[GA][CT]^CG
1209 CGACCG
BsmBI 571 GAGACG CGTCTC(1/5)
860 GAGACG
BstUI 106 CGCG CG^CG
720 CGCG
EcoT14I 497 CCATGG C^C[AT][AT]GG
1496 CCATGG
HgaI 718 GACGC GACGC(5/10)
452 GCGTC
HphI 790 GGTGA GGTGA(8/7)
1599 GGTGA
KasI 66 GGCGCC G^GCGCC
576 GGCGCC
MaeII 863 ACGT A^CGT
1229 ACGT
MvnI 106 CGCG CG^CG
720 CGCG
NarI 66 GGCGCC GG^CGCC
576 GGCGCC
NcoI 497 CCATGG C^CATGG
1496 CCATGG
NspI 390 ACATGC [GA]CATG^[CT]
1490 ACATGT
PstI 235 CTGCAG CTGCA^G
1219 CTGCAG
PvuII 399 CAGCTG CAG^CTG
513 CAGCTG
SfcI 235 CTGCAG C^T[GA][CT]AG
1219 CTGCAG
SfoI 66 GGCGCC GGC^GCC
576 GGCGCC
StyI 497 CCATGG C^C[AT][AT]GG
1496 CCATGG
TaqI 37 TCGA T^CGA
1208 TCGA
TthHB8I 37 TCGA T^CGA
1208 TCGA
XcmI 126 CCAGCGCCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
683 CCAGCACGGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcyI AfaI AluI ApaI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TseI Tsp45I
TspRI