(Circular) (Six-base) MAPSORT of: pFN21AE9288.seq Check: 602 from: 1 to: 6322 pFN21AE9288 6322 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2871 4132 278 Size: 53 83 186 936 1335 1261 2468 Fragments arranged by size: 2468 1335 1261 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3665 1937 Size: 1728 4594 Acc65I G'GTAC_C Cuts at: 3643 3643 Size: 6322 AclI AA'CG_TT Cuts at: 1563 4449 4822 1563 Size: 2886 373 3063 Fragments arranged by size: 3063 2886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2411 3012 4385 5769 6037 1632 Size: 779 601 1373 1384 268 1917 Fragments arranged by size: 1917 1384 1373 779 601 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6125 AflIII A'CryG_T Cuts at: 1203 3495 5221 6198 1203 Size: 2292 1726 977 1327 Fragments arranged by size: 2292 1726 1327 977 AgeI A'CCGG_T Cuts at: 5153 5153 Size: 6322 AhdI GACnn_n'nnGTC Cuts at: 5051 5051 Size: 6322 AleI CACnn'nnGTG Cuts at: 1166 2696 3107 3254 1166 Size: 1530 411 147 4234 Fragments arranged by size: 4234 1530 411 147 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2412 1184 Size: 1228 5094 AlwNI CAG_nnn'CTG Cuts at: 2137 2365 4065 5637 2137 Size: 228 1700 1572 2822 Fragments arranged by size: 2822 1700 1572 228 ApaI G_GGCC'C Cuts at: 2416 2736 2782 2416 Size: 320 46 5956 Fragments arranged by size: 5956 320 46 ApaLI G'TGCA_C Cuts at: 3506 4379 5535 3506 Size: 873 1156 4293 Fragments arranged by size: 4293 1156 873 ApoI r'AATT_y Cuts at: 1493 3003 3631 3857 4009 6118 1493 Size: 1510 628 226 152 2109 1697 Fragments arranged by size: 2109 1697 1510 628 226 152 AseI AT'TA_AT Cuts at: 160 1032 4876 160 Size: 872 3844 1606 Fragments arranged by size: 3844 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 AvaI C'yCGr_G Cuts at: 1870 1943 2041 3647 1870 Size: 73 98 1606 4545 Fragments arranged by size: 4545 1606 98 73 BamHI G'GATC_C Cuts at: 3652 4019 3652 Size: 367 5955 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2071 2294 2581 3643 5098 Size: 325 217 627 284 223 287 1062 1455 Cuts at: 5098 618 Size: 1842 Fragments arranged by size: 1842 1455 1062 627 325 287 284 223 217 BanII G_rGCy'C Cuts at: 1391 2416 2736 2782 3578 3641 1391 Size: 1025 320 46 796 63 4072 Fragments arranged by size: 4072 1025 796 320 63 46 BbeI G_GCGC'C Cuts at: 2075 2585 2075 Size: 510 5812 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2904 928 Size: 971 1005 4346 Fragments arranged by size: 4346 1005 971 BbvCI CC'TCA_GC Cuts at: 2754 2754 Size: 6322 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2032 2066 4513 4547 1903 Size: 34 95 34 2447 34 3678 Fragments arranged by size: 3678 2447 95 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2500 4214 5424 2500 Size: 1714 1210 3398 Fragments arranged by size: 3398 1714 1210 BclI T'GATC_A Cuts at: 1548 2343 1548 Size: 795 5527 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2261 2370 4933 Size: 107 122 71 631 605 589 109 2563 Cuts at: 4933 136 Size: 1525 Fragments arranged by size: 2563 1525 631 605 589 122 109 107 71 BglII A'GATC_T Cuts at: 6317 6317 Size: 6322 BlpI GC'TnA_GC Cuts at: 3739 3739 Size: 6322 Bme1580I G_kGCm'C Cuts at: 1790 2299 2416 2736 2782 3510 4383 5539 1790 Size: 509 117 320 46 728 873 1156 2573 Fragments arranged by size: 2573 1156 873 728 509 320 117 46 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6322 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2197 2353 2654 Size: 416 53 83 399 24 780 156 301 Cuts at: 2654 2902 4053 5011 442 Size: 248 1151 958 1753 Fragments arranged by size: 1753 1151 958 780 416 399 301 248 156 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2097 2500 3566 4982 1239 Size: 858 403 1066 1416 2579 Fragments arranged by size: 2579 1416 1066 858 403 Bpu10I CC'TnA_GC Cuts at: 2754 2754 Size: 6322 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2213 2893 3380 4447 5312 5610 2213 Size: 680 487 1067 865 298 2925 Fragments arranged by size: 2925 1067 865 680 487 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2666 4985 882 Size: 617 1167 2319 2219 Fragments arranged by size: 2319 2219 1167 617 BsaAI yAC'GTr Cuts at: 493 1204 3235 4113 493 Size: 711 2031 878 2702 Fragments arranged by size: 2702 2031 878 711 BsaBI GATnn'nnATC Cuts at: 6316 6316 Size: 6322 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2072 2457 2582 2868 Size: 53 83 186 936 539 385 125 286 Cuts at: 2868 4129 4511 5146 275 Size: 1261 382 635 1451 Fragments arranged by size: 1451 1261 936 635 539 385 382 286 186 125 83 53 BsaWI w'CCGG_w Cuts at: 4755 5153 5427 5574 5985 4755 Size: 398 274 147 411 5092 Fragments arranged by size: 5092 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2158 2188 2312 2342 1178 Size: 30 165 30 755 30 124 30 5158 Fragments arranged by size: 5158 755 165 124 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2333 3077 3173 1186 Size: 934 213 744 96 4335 Fragments arranged by size: 4335 934 744 213 96 BseYI C'CCAG_C Cuts at: 2298 2595 2687 2703 5525 6021 2298 Size: 297 92 16 2822 496 2599 Fragments arranged by size: 2822 2599 496 297 92 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2351 3085 3397 1163 Size: 573 615 734 312 4088 Fragments arranged by size: 4088 734 615 573 312 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3108 3217 3772 4533 5561 1540 Size: 275 186 1107 109 555 761 1028 2301 Fragments arranged by size: 2301 1107 1028 761 555 275 186 109 BsiHKAI G_wGCw'C Cuts at: 2118 3153 3510 4383 4468 5539 2118 Size: 1035 357 873 85 1071 2901 Fragments arranged by size: 2901 1071 1035 873 357 85 BsiWI C'GTAC_G Cuts at: 2119 2119 Size: 6322 BsmI GAATG_Cn' Cuts at: 3833 3926 3833 Size: 93 6229 BsmBI CGTCTCn'nnnn_ Cuts at: 2570 2859 2570 Size: 289 6033 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2299 2416 2736 2782 3153 3510 Size: 399 328 181 117 320 46 371 357 Cuts at: 3510 3578 3641 4383 4468 5539 1391 Size: 68 63 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 371 357 328 320 181 117 85 68 63 46 BspEI T'CCGG_A Cuts at: 5985 5985 Size: 6322 BspHI T'CATG_A Cuts at: 3207 4211 5941 3207 Size: 1004 1730 3588 Fragments arranged by size: 3588 1730 1004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3231 3677 844 Size: 2387 446 3489 Fragments arranged by size: 3489 2387 446 BsrBI CCG'CTC Cuts at: 1394 1823 3172 4209 1394 Size: 429 1349 1037 3507 Fragments arranged by size: 3507 1349 1037 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4817 4991 65 Size: 1183 3569 174 1396 Fragments arranged by size: 3569 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3459 4966 5153 1700 Size: 110 1649 1507 187 2869 Fragments arranged by size: 2869 1649 1507 187 110 BsrGI T'GTAC_A Cuts at: 96 3490 96 Size: 3394 2928 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6322 BssSI C'ACGA_G Cuts at: 3155 4382 5394 3155 Size: 1227 1012 4083 Fragments arranged by size: 4083 1227 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2193 794 Size: 1399 4923 BstBI TT'CG_AA Cuts at: 5126 5126 Size: 6322 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6322 BstYI r'GATC_y Cuts at: 1972 2338 3652 4019 4404 4421 5873 5959 5971 Size: 366 1314 367 385 17 1452 86 12 Cuts at: 5971 6317 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1314 385 367 366 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2026 2502 2564 2585 3501 513 Size: 552 939 22 476 62 21 916 3334 Fragments arranged by size: 3334 939 916 552 476 62 22 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5583 Fragments arranged by size: 5583 683 56 BtsI GCAGTG_nn' Cuts at: 3359 3838 4632 4652 3359 Size: 479 794 20 5029 Fragments arranged by size: 5029 794 479 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6322 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6287 DraI TTT'AAA Cuts at: 3626 3626 Size: 6322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5329 809 Size: 507 4013 1802 Fragments arranged by size: 4013 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2400 2561 3105 Size: 54 1425 50 275 18 570 161 544 Cuts at: 3105 3457 3769 4658 6287 8 Size: 352 312 889 1629 43 Fragments arranged by size: 1629 1425 889 570 544 352 312 275 161 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3105 3769 1537 Size: 275 1293 664 4090 Fragments arranged by size: 4090 1293 664 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2957 4252 1353 Size: 1604 1295 3423 Fragments arranged by size: 3423 1604 1295 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2144 3566 4909 5283 5429 387 Size: 1064 138 555 1422 1343 374 146 1280 Fragments arranged by size: 1422 1343 1280 1064 555 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 2411 2500 3012 3566 4385 4982 Size: 393 465 314 89 512 554 819 597 Cuts at: 4982 5769 6037 1239 Size: 787 268 1524 Fragments arranged by size: 1524 819 787 597 554 512 465 393 314 268 89 EcoNI CCTnn'n_nnAGG Cuts at: 2858 2858 Size: 6322 EcoO109I rG'GnC_Cy Cuts at: 2161 2412 2413 2655 2732 2778 2779 3020 3535 Size: 251 1 242 77 46 1 241 515 Cuts at: 3535 2161 Size: 4948 Fragments arranged by size: 4948 515 251 242 241 77 46 1 1 EcoRI G'AATT_C Cuts at: 3003 3631 4009 3003 Size: 628 378 5316 Fragments arranged by size: 5316 628 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2548 2548 Size: 6322 FspI TGC'GCA Cuts at: 2054 4828 2054 Size: 2774 3548 HaeII r_GCGC'y Cuts at: 1678 2075 2137 2585 3123 3232 5469 1678 Size: 397 62 448 538 109 2237 2531 Fragments arranged by size: 2531 2237 538 448 397 109 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 2498 2530 4963 Size: 32 135 32 509 32 385 32 2433 Cuts at: 4963 4995 5037 5069 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2433 509 385 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2251 3666 3912 669 Size: 988 281 313 1415 246 3079 Fragments arranged by size: 3079 1415 988 313 281 246 HindIII A'AGCT_T Cuts at: 748 3044 748 Size: 2296 4026 HpaI GTT'AAC Cuts at: 3912 3912 Size: 6322 KasI G'GCGC_C Cuts at: 2071 2581 2071 Size: 510 5812 KpnI G_GTAC'C Cuts at: 3647 3647 Size: 6322 MfeI C'AATT_G Cuts at: 3921 3921 Size: 6322 MluI A'CGCG_T Cuts at: 6198 6198 Size: 6322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5436 5620 1305 Size: 4131 184 2007 Fragments arranged by size: 4131 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2563 10 Size: 54 1425 343 731 3769 Fragments arranged by size: 3769 1425 731 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2394 2696 3107 3254 3500 4280 4639 Size: 648 1228 302 411 147 246 780 359 Cuts at: 4639 4798 518 Size: 159 2042 Fragments arranged by size: 2042 1228 780 648 411 359 302 246 159 147 MspA1I CmG'CkG Cuts at: 1606 2406 2520 3301 3385 3514 3738 4415 5563 Size: 800 114 781 84 129 224 677 1148 Cuts at: 5563 5808 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 800 781 677 245 224 129 114 84 NaeI GCC'GGC Cuts at: 3461 3461 Size: 6322 NarI GG'CG_CC Cuts at: 2072 2582 2072 Size: 510 5812 NcoI C'CATG_G Cuts at: 513 1065 2004 2502 3501 513 Size: 552 939 498 999 3334 Fragments arranged by size: 3334 999 939 552 498 NdeI CA'TA_TG Cuts at: 387 387 Size: 6322 NgoMIV G'CCGG_C Cuts at: 3459 3459 Size: 6322 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6322 NotI GC'GGCC_GC Cuts at: 3769 3769 Size: 6322 NspI r_CATG'y Cuts at: 1132 1229 2399 3499 3680 5225 1132 Size: 97 1170 1100 181 1545 2229 Fragments arranged by size: 2229 1545 1170 1100 181 97 PasI CC'CwG_GG Cuts at: 1348 2615 2638 2783 1348 Size: 1267 23 145 4887 Fragments arranged by size: 4887 1267 145 23 PciI A'CATG_T Cuts at: 3495 5221 3495 Size: 1726 4596 PflMI CCAn_nnn'nTGG Cuts at: 1108 3563 1108 Size: 2455 3867 PfoI T'CCnGG_A Cuts at: 3165 3165 Size: 6322 PmeI GTTT'AAAC Cuts at: 3626 3626 Size: 6322 PmlI CAC'GTG Cuts at: 3235 3235 Size: 6322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4391 4423 5904 5936 4391 Size: 32 1481 32 4777 Fragments arranged by size: 4777 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2161 2655 3020 2161 Size: 494 365 5463 Fragments arranged by size: 5463 494 365 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6322 PsiI TTA'TAA Cuts at: 3892 3892 Size: 6322 PspOMI G'GGCC_C Cuts at: 2412 2732 2778 2412 Size: 320 46 5956 Fragments arranged by size: 5956 320 46 PstI C_TGCA'G Cuts at: 830 2244 3228 3674 830 Size: 1414 984 446 3478 Fragments arranged by size: 3478 1414 984 446 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6322 PvuII CAG'CTG Cuts at: 2406 2520 2406 Size: 114 6208 SalI G'TCGA_C Cuts at: 1936 3664 1936 Size: 1728 4594 SanDI GG'GwC_CC Cuts at: 2655 3020 2655 Size: 365 5957 SbfI CC_TGCA'GG Cuts at: 3674 3674 Size: 6322 ScaI AGT'ACT Cuts at: 2450 4570 6282 2450 Size: 2120 1712 2490 Fragments arranged by size: 2490 2120 1712 SfcI C'TryA_G Cuts at: 826 1045 2240 3224 3670 4805 5486 5677 826 Size: 219 1195 984 446 1135 681 191 1471 Fragments arranged by size: 1471 1195 1135 984 681 446 219 191 SfoI GGC'GCC Cuts at: 2073 2583 2073 Size: 510 5812 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6322 SmaI CCC'GGG Cuts at: 1872 3649 1872 Size: 1777 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2041 2228 2872 3395 4426 5327 Size: 197 926 98 187 644 523 1031 901 Cuts at: 5327 5589 820 Size: 262 1553 Fragments arranged by size: 1553 1031 926 901 644 523 262 197 187 98 SnaBI TAC'GTA Cuts at: 493 4113 493 Size: 3620 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6322 SphI G_CATG'C Cuts at: 1132 3680 1132 Size: 2548 3774 SspI AAT'ATT Cuts at: 5 52 4246 5 Size: 47 4194 2081 Fragments arranged by size: 4194 2081 47 StuI AGG'CCT Cuts at: 1517 2528 1517 Size: 1011 5311 StyI C'CwwG_G Cuts at: 513 1065 2004 2502 3501 3761 513 Size: 552 939 498 999 260 3074 Fragments arranged by size: 3074 999 939 552 498 260 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2374 2783 4349 4366 4519 4704 5149 Size: 1255 794 409 1566 17 153 185 445 Cuts at: 5149 325 Size: 1498 Fragments arranged by size: 1566 1498 1255 794 445 409 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2448 3490 4568 Size: 274 80 33 51 1443 471 1042 1078 Cuts at: 4568 6280 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1078 1042 471 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2158 2190 2266 2298 2312 2344 6282 6314 2158 Size: 32 76 32 14 32 3938 32 2166 Fragments arranged by size: 3938 2166 76 32 32 32 32 14 Tth111I GACn'n_nGTC Cuts at: 1316 2437 1316 Size: 1121 5201 XbaI T'CTAG_A Cuts at: 3658 3658 Size: 6322 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2138 2695 2138 Size: 557 5765 XhoI C'TCGA_G Cuts at: 1943 2041 1943 Size: 98 6224 XmaI C'CCGG_G Cuts at: 1870 3647 1870 Size: 1777 4545 XmnI GAAnn'nnTTC Cuts at: 4451 4451 Size: 6322 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2869 4130 276 Size: 53 83 186 936 1335 1261 2468 Fragments arranged by size: 2468 1335 1261 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI