(Linear) (Six-base) MAPSORT of: KIEE9288.seq Check: 872 from: 1 to: 1617 KIEE9288.orf With 182 enzymes: SgfI * December 5, 2014 13:29 .. AatII G_ACGT'C Cuts at: 0 866 1617 Size: 866 751 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 406 1007 1617 Size: 406 601 610 Fragments arranged by size: 610 601 406 AflIII A'CryG_T Cuts at: 0 1490 1617 Size: 1490 127 AleI CACnn'nnGTG Cuts at: 0 691 1102 1249 1617 Size: 691 411 147 368 Fragments arranged by size: 691 411 368 147 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 407 1617 Size: 407 1210 AlwNI CAG_nnn'CTG Cuts at: 0 132 360 1617 Size: 132 228 1257 Fragments arranged by size: 1257 228 132 ApaI G_GGCC'C Cuts at: 0 411 731 777 1617 Size: 411 320 46 840 Fragments arranged by size: 840 411 320 46 ApaLI G'TGCA_C Cuts at: 0 1501 1617 Size: 1501 116 ApoI r'AATT_y Cuts at: 0 998 1617 Size: 998 619 AvaI C'yCGr_G Cuts at: 0 36 1617 Size: 36 1581 BanI G'GyrC_C Cuts at: 0 66 289 576 1617 Size: 66 223 287 1041 Fragments arranged by size: 1041 287 223 66 BanII G_rGCy'C Cuts at: 0 411 731 777 1573 1617 Size: 411 320 46 796 44 Fragments arranged by size: 796 411 320 46 44 BbeI G_GCGC'C Cuts at: 0 70 580 1617 Size: 70 510 1037 Fragments arranged by size: 1037 510 70 BbsI GAAGACnn'nnnn_ Cuts at: 0 899 1617 Size: 899 718 BbvCI CC'TCA_GC Cuts at: 0 749 1617 Size: 749 868 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 27 61 1617 Size: 27 34 1556 Fragments arranged by size: 1556 34 27 BciVI GTATCCnnnnn_n' Cuts at: 0 495 1617 Size: 495 1122 BclI T'GATC_A Cuts at: 0 338 1617 Size: 338 1279 BglI GCCn_nnn'nGGC Cuts at: 0 256 365 1617 Size: 256 109 1252 Fragments arranged by size: 1252 256 109 Bme1580I G_kGCm'C Cuts at: 0 294 411 731 777 1505 1617 Size: 294 117 320 46 728 112 Fragments arranged by size: 728 320 294 117 112 46 BmrI ACTGGGnnnn_n' Cuts at: 0 192 348 649 897 1617 Size: 192 156 301 248 720 Fragments arranged by size: 720 301 248 192 156 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 92 495 1561 1617 Size: 92 403 1066 56 Fragments arranged by size: 1066 403 92 56 Bpu10I CC'TnA_GC Cuts at: 0 749 1617 Size: 749 868 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 888 1375 1617 Size: 208 680 487 242 Fragments arranged by size: 680 487 242 208 BsaI GGTCTCn'nnnn_ Cuts at: 0 661 1617 Size: 661 956 BsaAI yAC'GTr Cuts at: 0 1230 1617 Size: 1230 387 BsaHI Gr'CG_yC Cuts at: 0 67 452 577 863 1617 Size: 67 385 125 286 754 Fragments arranged by size: 754 385 286 125 67 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 153 183 307 337 1617 Size: 153 30 124 30 1280 Fragments arranged by size: 1280 153 124 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 115 328 1072 1168 1617 Size: 115 213 744 96 449 Fragments arranged by size: 744 449 213 115 96 BseYI C'CCAG_C Cuts at: 0 293 590 682 698 1617 Size: 293 297 92 16 919 Fragments arranged by size: 919 297 293 92 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 346 1080 1392 1617 Size: 346 734 312 225 Fragments arranged by size: 734 346 312 225 BsiEI CG_ry'CG Cuts at: 0 1103 1212 1617 Size: 1103 109 405 Fragments arranged by size: 1103 405 109 BsiHKAI G_wGCw'C Cuts at: 0 113 1148 1505 1617 Size: 113 1035 357 112 Fragments arranged by size: 1035 357 113 112 BsiWI C'GTAC_G Cuts at: 0 114 1617 Size: 114 1503 BsmBI CGTCTCn'nnnn_ Cuts at: 0 565 854 1617 Size: 565 289 763 Fragments arranged by size: 763 565 289 Bsp1286I G_dGCh'C Cuts at: 0 113 294 411 731 777 1148 1505 1573 Size: 113 181 117 320 46 371 357 68 Cuts at: 1573 1617 Size: 44 Fragments arranged by size: 371 357 320 181 117 113 68 46 44 BspHI T'CATG_A Cuts at: 0 1202 1617 Size: 1202 415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1226 1617 Size: 1226 391 BsrBI CCG'CTC Cuts at: 0 1167 1617 Size: 1167 450 BsrFI r'CCGG_y Cuts at: 0 1454 1617 Size: 1454 163 BsrGI T'GTAC_A Cuts at: 0 1485 1617 Size: 1485 132 BssSI C'ACGA_G Cuts at: 0 1150 1617 Size: 1150 467 BstAPI GCAn_nnn'nTGC Cuts at: 0 188 1617 Size: 188 1429 BstYI r'GATC_y Cuts at: 0 333 1617 Size: 333 1284 BtgI C'CryG_G Cuts at: 0 21 497 559 580 1496 1617 Size: 21 476 62 21 916 121 Fragments arranged by size: 916 476 121 62 21 21 BtsI GCAGTG_nn' Cuts at: 0 1354 1617 Size: 1354 263 EaeI y'GGCC_r Cuts at: 0 395 556 1100 1452 1617 Size: 395 161 544 352 165 Fragments arranged by size: 544 395 352 165 161 EagI C'GGCC_G Cuts at: 0 1100 1617 Size: 1100 517 EarI CTCTTCn'nnn_ Cuts at: 0 952 1617 Size: 952 665 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 139 1561 1617 Size: 139 1422 56 Fragments arranged by size: 1422 139 56 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 92 406 495 1007 1561 1617 Size: 92 314 89 512 554 56 Fragments arranged by size: 554 512 314 92 89 56 EcoNI CCTnn'n_nnAGG Cuts at: 0 853 1617 Size: 853 764 EcoO109I rG'GnC_Cy Cuts at: 0 156 407 408 650 727 773 774 1015 Size: 156 251 1 242 77 46 1 241 Cuts at: 1015 1530 1617 Size: 515 87 Fragments arranged by size: 515 251 242 241 156 87 77 46 1 1 EcoRI G'AATT_C Cuts at: 0 998 1617 Size: 998 619 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 543 1617 Size: 543 1074 FspI TGC'GCA Cuts at: 0 49 1617 Size: 49 1568 HaeII r_GCGC'y Cuts at: 0 70 132 580 1118 1227 1617 Size: 70 62 448 538 109 390 Fragments arranged by size: 538 448 390 109 70 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 76 108 493 525 1617 Size: 76 32 385 32 1092 Fragments arranged by size: 1092 385 76 32 32 HincII GTy'rAC Cuts at: 0 246 1617 Size: 246 1371 HindIII A'AGCT_T Cuts at: 0 1039 1617 Size: 1039 578 KasI G'GCGC_C Cuts at: 0 66 576 1617 Size: 66 510 1041 Fragments arranged by size: 1041 510 66 MscI TGG'CCA Cuts at: 0 558 1617 Size: 558 1059 MslI CAynn'nnrTG Cuts at: 0 389 691 1102 1249 1495 1617 Size: 389 302 411 147 246 122 Fragments arranged by size: 411 389 302 246 147 122 MspA1I CmG'CkG Cuts at: 0 401 515 1296 1380 1509 1617 Size: 401 114 781 84 129 108 Fragments arranged by size: 781 401 129 114 108 84 NaeI GCC'GGC Cuts at: 0 1456 1617 Size: 1456 161 NarI GG'CG_CC Cuts at: 0 67 577 1617 Size: 67 510 1040 Fragments arranged by size: 1040 510 67 NcoI C'CATG_G Cuts at: 0 497 1496 1617 Size: 497 999 121 Fragments arranged by size: 999 497 121 NgoMIV G'CCGG_C Cuts at: 0 1454 1617 Size: 1454 163 NspI r_CATG'y Cuts at: 0 394 1494 1617 Size: 394 1100 123 Fragments arranged by size: 1100 394 123 PasI CC'CwG_GG Cuts at: 0 610 633 778 1617 Size: 610 23 145 839 Fragments arranged by size: 839 610 145 23 PciI A'CATG_T Cuts at: 0 1490 1617 Size: 1490 127 PflMI CCAn_nnn'nTGG Cuts at: 0 1558 1617 Size: 1558 59 PfoI T'CCnGG_A Cuts at: 0 1160 1617 Size: 1160 457 PmlI CAC'GTG Cuts at: 0 1230 1617 Size: 1230 387 PpuMI rG'GwC_Cy Cuts at: 0 156 650 1015 1617 Size: 156 494 365 602 Fragments arranged by size: 602 494 365 156 PspOMI G'GGCC_C Cuts at: 0 407 727 773 1617 Size: 407 320 46 844 Fragments arranged by size: 844 407 320 46 PstI C_TGCA'G Cuts at: 0 239 1223 1617 Size: 239 984 394 Fragments arranged by size: 984 394 239 PvuII CAG'CTG Cuts at: 0 401 515 1617 Size: 401 114 1102 Fragments arranged by size: 1102 401 114 SanDI GG'GwC_CC Cuts at: 0 650 1015 1617 Size: 650 365 602 Fragments arranged by size: 650 602 365 ScaI AGT'ACT Cuts at: 0 445 1617 Size: 445 1172 SfcI C'TryA_G Cuts at: 0 235 1219 1617 Size: 235 984 398 Fragments arranged by size: 984 398 235 SfoI GGC'GCC Cuts at: 0 68 578 1617 Size: 68 510 1039 Fragments arranged by size: 1039 510 68 SmlI C'TyrA_G Cuts at: 0 36 223 867 1390 1617 Size: 36 187 644 523 227 Fragments arranged by size: 644 523 227 187 36 StuI AGG'CCT Cuts at: 0 523 1617 Size: 523 1094 StyI C'CwwG_G Cuts at: 0 497 1496 1617 Size: 497 999 121 Fragments arranged by size: 999 497 121 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 369 778 1617 Size: 369 409 839 Fragments arranged by size: 839 409 369 TatI w'GTAC_w Cuts at: 0 443 1485 1617 Size: 443 1042 132 Fragments arranged by size: 1042 443 132 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 153 185 261 293 307 339 1617 Size: 153 32 76 32 14 32 1278 Fragments arranged by size: 1278 153 76 32 32 32 14 Tth111I GACn'n_nGTC Cuts at: 0 432 1617 Size: 432 1185 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 133 690 1617 Size: 133 557 927 Fragments arranged by size: 927 557 133 XhoI C'TCGA_G Cuts at: 0 36 1617 Size: 36 1581 ZraI GAC'GTC Cuts at: 0 864 1617 Size: 864 753 Enzymes that do cut: AatII AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstYI BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SanDI ScaI SfcI SfoI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AgeI AhdI AloI AscI AseI AsiSI AvrII BaeI BamHI BfrBI BglII BlpI BmgBI BmtI BplI BsaBI BsaWI BsmI BspEI BsrDI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRV FseI FspAI HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XmaI XmnI