################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BseAI BseRI BsgI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 163 GACGTC GACGT^C AcsI 318 GAATTT [GA]^AATT[CT] Alw44I 153 GTGCAC G^TGCAC AlwI 242 GGATC GGATC(4/5) ApaLI 153 GTGCAC G^TGCAC ApoI 318 GAATTT [GA]^AATT[CT] AspHI 153 GTGCAC G[AT]GC[AT]^C AvaII 218 GGTCC G^G[AT]CC BalI 2 TGGCCA TGG^CCA BbrPI 237 CACGTG CAC^GTG BclI 338 TGATCA T^GATCA BmyI 153 GTGCAC G[AGT]GC[ACT]^C BpmI 358 CTGGAG CTGGAG(16/14) BsaAI 237 CACGTG [CT]AC^GT[GA] BsaWI 167 TCCGGT [AT]^CCGG[AT] BsiEI 65 CGGCCG CG[GA][CT]^CG BsiHKAI 153 GTGCAC G[AT]GC[AT]^C Bsp1286I 153 GTGCAC G[AGT]GC[ACT]^C BsrBI 125 GAGCGG CCGCTC(-3/-3) BssHII 270 GCGCGC G^CGCGC Cfr13I 218 GGTCC G^G[ACGT]CC DdeI 326 CTAAG C^T[ACGT]AG DraI 85 TTTAAA TTT^AAA EagI 65 CGGCCG C^GGCCG EclXI 65 CGGCCG C^GGCCG Eco52I 65 CGGCCG C^GGCCG EcoNI 297 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 338 TGATCA T^GATCA HgaI 412 GACGC GACGC(5/10) MaeIII 79 GTGAC ^GT[ACGT]AC MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MseI 86 TTAA T^TAA MslI 72 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 258 CAGCGG C[AC]G^C[GT]G NlaIII 207 CATG CATG^ PmaCI 237 CACGTG CAC^GTG PmlI 237 CACGTG CAC^GTG PstI 265 CTGCAG CTGCA^G Sau96I 218 GGTCC G^G[ACGT]CC SexAI 44 ACCTGGT A^CC[AT]GGT SfaNI 202 GATGC GCATC(5/9) SfcI 265 CTGCAG C^T[GA][CT]AG TaqI 14 TCGA T^CGA Tru9I 86 TTAA T^TAA Tsp45I 79 GTGAC ^GT[GC]AC TthHB8I 14 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 52 CGCG CG^CG 271 CGCG AcyI 163 GACGTC G[GA]^CG[CT]C 412 GACGCC AluI 356 AGCT AG^CT 383 AGCT BbvI 267 GCAGC GCAGC(8/12) 264 GCTGC BsaHI 163 GACGTC G[GA]^CG[CT]C 412 GACGCC BsmFI 283 GGGAC GGGAC(10/14) 109 GTCCC BstF5I 144 CATCC GGATG(2/0) 201 GGATG BstNI 45 CCTGG CC^[AT]GG 297 CCTGG BstUI 52 CGCG CG^CG 271 CGCG EaeI 2 TGGCCA [CT]^GGCC[GA] 65 CGGCCG EcoRII 45 CCTGG ^CC[AT]GG 297 CCTGG FauI 260 GCGGG CCCGC(4/6) 394 GCGGG FokI 144 CATCC GGATG(9/13) 201 GGATG HaeIII 3 GGCC GG^CC 66 GGCC Hin1I 163 GACGTC G[GA]^CG[CT]C 412 GACGCC MboII 364 GAAGA GAAGA(8/7) 387 GAAGA MvaI 45 CCTGG CC^[AT]GG 297 CCTGG MvnI 52 CGCG CG^CG 271 CGCG NlaIV 97 GGCTCC GG[ACGT]^[ACGT]CC 400 GGAGCC TseI 264 GCTGC G^C[AT]GC 267 GCAGC Tsp509I 319 AATT ^AATT 406 AATT TspRI 151 CAGTG CA[GC]TG[ACGT][ACGT]^ 342 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AciI AfaI BcnI BsaJI BsiYI BslI BssKI Cac8I CfoI DpnII Fnu4HI HapII HhaI HinP1I HpaII HphI ItaI MaeII MboI MnlI MspI MwoI NciI NdeII RsaI Sau3AI ScrFI