################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I XbaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AhdI 211 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1018 GTGCAC G^TGCAC ApaLI 1018 GTGCAC G^TGCAC AspEI 211 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1391 TGGCCA TGG^CCA BamHI 1242 GGATCC G^GATCC BbrPI 355 CACGTG CAC^GTG BlpI 1257 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1257 GCTGAGC GC^T[ACGT]AGC BseRI 6 CTCCTC GAGGAG(10/8) BsgI 1369 GTGCAG GTGCAG(16/14) BsiEI 890 CGGCCG CG[GA][CT]^CG BspMI 98 ACCTGC ACCTGC(4/8) BsrBI 666 GAGCGG CCGCTC(-3/-3) BstAPI 1089 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1242 GGATCC [GA]^GATC[CT] CelII 1257 GCTGAGC GC^T[ACGT]AGC DraIII 1012 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 890 CGGCCG C^GGCCG Eam1105I 211 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 890 CGGCCG C^GGCCG Eco52I 890 CGGCCG C^GGCCG HgaI 895 GACGC GACGC(5/10) HinfI 643 GACTC G^A[ACGT]TC MflI 1242 GGATCC [GA]^GATC[CT] MluNI 1391 TGGCCA TGG^CCA MscI 1391 TGGCCA TGG^CCA PaeR7I 1138 CTCGAG C^TCGAG PleI 643 GACTC GAGTC(4/5) PmaCI 355 CACGTG CAC^GTG PmlI 355 CACGTG CAC^GTG PstI 157 CTGCAG CTGCA^G SacI 3 GAGCTC GAGCT^C SfiI 226 GGCCTCGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1138 CTCGAG C^T[CT][GA]AG StuI 225 AGGCCT AGG^CCT XhoI 1138 CTCGAG C^TCGAG XhoII 1242 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 876 CGCG CG^CG 897 CGCG AcyI 28 GGCGCC G[GA]^CG[CT]C 1378 GGCGCC ApaI 635 GGGCCC GGGCC^C 713 GGGCCC AspI 856 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 895 GACGCGGTC BbeI 28 GGCGCC GGCGC^C 1378 GGCGCC BbsI 1111 GAAGAC GAAGAC(2/6) 491 GTCTTC BpuAI 1111 GAAGAC GAAGAC(2/6) 491 GTCTTC BsaAI 355 CACGTG [CT]AC^GT[GA] 544 TACGTG BsaHI 28 GGCGCC G[GA]^CG[CT]C 1378 GGCGCC BsaWI 332 ACCGGA [AT]^CCGG[AT] 580 TCCGGT BsrI 467 ACTGG ACTGG(1/-1) 432 CCAGT BstEII 840 GGTGACC G^GT[ACGT]ACC 1069 GGTTACC BstPI 840 GGTGACC G^GT[ACGT]ACC 1069 GGTTACC BstUI 876 CGCG CG^CG 897 CGCG BstXI 824 CCACAAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1277 CCATTGACGTGG DrdI 451 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 541 GACTACGTGGTC EarI 655 CTCTTC CTCTTC(1/4) 485 GAAGAG EcoO65I 840 GGTGACC G^GT[ACGT]ACC 1069 GGTTACC Hin1I 28 GGCGCC G[GA]^CG[CT]C 1378 GGCGCC KasI 28 GGCGCC G^GCGCC 1378 GGCGCC Ksp632I 655 CTCTTC CTCTTC(1/4) 485 GAAGAG MvnI 876 CGCG CG^CG 897 CGCG NarI 28 GGCGCC GG^CGCC 1378 GGCGCC NcoI 410 CCATGG C^CATGG 1331 CCATGG PflFI 856 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 895 GACGCGGTC PflMI 201 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 432 CCAGTGGATGG SfoI 28 GGCGCC GGC^GCC 1378 GGCGCC Tsp45I 592 GTCAC ^GT[GC]AC 841 GTGAC Tth111I 856 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 895 GACGCGGTC Van91I 201 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 432 CCAGTGGATGG XcmI 347 CCATCCCCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1331 CCATGGGGCCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TseI TspRI TthHB8I XmaCI XmaI