(Circular) (Six-base) MAPSORT of: pFN21AE5631.seq Check: 8137 from: 1 to: 6136 pFN21AE5631 6136 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3946 278 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3479 1937 Size: 1542 4594 Acc65I G'GTAC_C Cuts at: 3457 3457 Size: 6136 AclI AA'CG_TT Cuts at: 1563 4263 4636 1563 Size: 2700 373 3063 Fragments arranged by size: 3063 2700 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4199 5583 5851 1632 Size: 2567 1384 268 1917 Fragments arranged by size: 2567 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5939 AflIII A'CryG_T Cuts at: 1203 5035 6012 1203 Size: 3832 977 1327 Fragments arranged by size: 3832 1327 977 AgeI A'CCGG_T Cuts at: 4967 4967 Size: 6136 AhdI GACnn_n'nnGTC Cuts at: 2221 4865 2221 Size: 2644 3492 AleI CACnn'nnGTG Cuts at: 1166 2603 2834 1166 Size: 1437 231 4468 Fragments arranged by size: 4468 1437 231 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6136 AlwNI CAG_nnn'CTG Cuts at: 2212 2634 3103 3233 3262 3879 5451 2212 Size: 422 469 130 29 617 1572 2897 Fragments arranged by size: 2897 1572 617 469 422 130 29 ApaI G_GGCC'C Cuts at: 2644 2722 2644 Size: 78 6058 ApaLI G'TGCA_C Cuts at: 3023 4193 5349 3023 Size: 1170 1156 3810 Fragments arranged by size: 3810 1170 1156 ApoI r'AATT_y Cuts at: 1493 3445 3671 3823 5932 1493 Size: 1952 226 152 2109 1697 Fragments arranged by size: 2109 1952 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4690 160 Size: 872 3658 1606 Fragments arranged by size: 3658 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 AvaI C'yCGr_G Cuts at: 1870 1943 2185 2384 2777 3008 3143 3461 1870 Size: 73 242 199 393 231 135 318 4545 Fragments arranged by size: 4545 393 318 242 231 199 135 73 BamHI G'GATC_C Cuts at: 3247 3466 3833 3247 Size: 219 367 5550 Fragments arranged by size: 5550 367 219 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 3093 3383 3457 4912 Size: 325 217 627 246 1060 290 74 1455 Cuts at: 4912 618 Size: 1842 Fragments arranged by size: 1842 1455 1060 627 325 290 246 217 74 BanII G_rGCy'C Cuts at: 1391 2012 2052 2451 2644 2722 3455 1391 Size: 621 40 399 193 78 733 4072 Fragments arranged by size: 4072 733 621 399 193 78 40 BbeI G_GCGC'C Cuts at: 2037 3387 2037 Size: 1350 4786 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2489 3123 928 Size: 971 590 634 3941 Fragments arranged by size: 3941 971 634 590 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4327 4361 1903 Size: 34 2390 34 3678 Fragments arranged by size: 3678 2390 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4028 5238 4028 Size: 1210 4926 BclI T'GATC_A Cuts at: 1548 1548 Size: 6136 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2238 2822 3324 Size: 107 122 71 631 605 566 584 502 Cuts at: 3324 4747 136 Size: 1423 1525 Fragments arranged by size: 1525 1423 631 605 584 566 502 122 107 71 BglII A'GATC_T Cuts at: 6131 6131 Size: 6136 BlpI GC'TnA_GC Cuts at: 3263 3553 3263 Size: 290 5846 Bme1580I G_kGCm'C Cuts at: 1790 2644 2722 3027 4197 5353 1790 Size: 854 78 305 1170 1156 2573 Fragments arranged by size: 2573 1170 1156 854 305 78 BmgBI CAC'GTC Cuts at: 1318 3289 1318 Size: 1971 4165 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3867 4825 442 Size: 416 53 83 399 24 2450 958 1753 Fragments arranged by size: 2450 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1999 2480 2635 2840 4796 1239 Size: 760 481 155 205 1956 2579 Fragments arranged by size: 2579 1956 760 481 205 155 Bpu10I CC'TnA_GC Cuts at: 2426 3410 2426 Size: 984 5152 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4261 5126 5424 4261 Size: 865 298 4973 Fragments arranged by size: 4973 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2197 3175 3413 4799 882 Size: 617 698 978 238 1386 2219 Fragments arranged by size: 2219 1386 978 698 617 238 BsaAI yAC'GTr Cuts at: 493 1204 2362 2551 3927 493 Size: 711 1158 189 1376 2702 Fragments arranged by size: 2702 1376 1158 711 189 BsaBI GATnn'nnATC Cuts at: 6130 6130 Size: 6136 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2034 3384 3943 4325 Size: 53 83 186 936 501 1350 559 382 Cuts at: 4325 4960 275 Size: 635 1451 Fragments arranged by size: 1451 1350 936 635 559 501 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2337 2585 4569 4967 5241 5388 5799 2337 Size: 248 1984 398 274 147 411 2674 Fragments arranged by size: 2674 1984 411 398 274 248 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2093 2123 3314 3344 1178 Size: 30 165 30 690 30 1191 30 3970 Fragments arranged by size: 3970 1191 690 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2002 1186 Size: 816 5320 BseYI C'CCAG_C Cuts at: 2627 2697 2767 3059 5339 5835 2627 Size: 70 70 292 2280 496 2928 Fragments arranged by size: 2928 2280 496 292 70 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3395 1163 Size: 573 1659 3904 Fragments arranged by size: 3904 1659 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2898 3586 4347 5375 1540 Size: 275 186 897 688 761 1028 2301 Fragments arranged by size: 2301 1028 897 761 688 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2349 3027 3314 4197 4282 5353 2012 Size: 337 678 287 883 85 1071 2795 Fragments arranged by size: 2795 1071 883 678 337 287 85 BsmI GAATG_Cn' Cuts at: 3647 3740 3647 Size: 93 6043 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2052 2349 2451 2644 2722 3027 Size: 399 222 40 297 102 193 78 305 Cuts at: 3027 3314 3455 4197 4282 5353 1391 Size: 287 141 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 305 297 287 222 193 141 102 85 78 40 BspEI T'CCGG_A Cuts at: 5799 5799 Size: 6136 BspHI T'CATG_A Cuts at: 4025 5755 4025 Size: 1730 4406 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2112 3491 844 Size: 1268 1379 3489 Fragments arranged by size: 3489 1379 1268 BsrBI CCG'CTC Cuts at: 1394 1823 2673 4023 1394 Size: 429 850 1350 3507 Fragments arranged by size: 3507 1350 850 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4631 4805 65 Size: 1183 3383 174 1396 Fragments arranged by size: 3383 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4780 4967 1700 Size: 110 2970 187 2869 Fragments arranged by size: 2970 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6136 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6136 BssSI C'ACGA_G Cuts at: 4196 5208 4196 Size: 1012 5124 BstAPI GCAn_nnn'nTGC Cuts at: 794 3100 794 Size: 2306 3830 BstBI TT'CG_AA Cuts at: 4940 4940 Size: 6136 BstEII G'GTnAC_C Cuts at: 1184 2845 3074 1184 Size: 1661 229 4246 Fragments arranged by size: 4246 1661 229 BstXI CCAn_nnnn'nTGG Cuts at: 2836 3289 2836 Size: 453 5683 BstYI r'GATC_y Cuts at: 1972 3247 3466 3833 4218 4235 5687 5773 5785 Size: 1275 219 367 385 17 1452 86 12 Cuts at: 5785 6131 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1275 385 367 346 219 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2256 2415 2604 2958 3336 513 Size: 552 1191 159 189 354 378 3313 Fragments arranged by size: 3313 1191 552 378 354 189 159 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2512 487 Size: 683 56 1286 4111 Fragments arranged by size: 4111 1286 683 56 BtsI GCAGTG_nn' Cuts at: 2514 3249 3652 4446 4466 2514 Size: 735 403 794 20 4184 Fragments arranged by size: 4184 794 735 403 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6136 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2420 2455 529 Size: 35 1856 35 4210 Fragments arranged by size: 4210 1856 35 35 DraI TTT'AAA Cuts at: 3440 3440 Size: 6136 DraIII CAC_nnn'GTG Cuts at: 3022 3022 Size: 6136 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2462 2552 5143 809 Size: 507 1146 90 2591 1802 Fragments arranged by size: 2591 1802 1146 507 90 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2895 3348 3396 Size: 54 1425 50 275 18 1065 453 48 Cuts at: 3396 3583 4472 6101 8 Size: 187 889 1629 43 Fragments arranged by size: 1629 1425 1065 889 453 275 187 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2895 3583 1537 Size: 275 1083 688 4090 Fragments arranged by size: 4090 1083 688 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2485 2666 4066 1353 Size: 1132 181 1400 3423 Fragments arranged by size: 3423 1400 1132 181 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2939 4723 5097 5243 387 Size: 1064 138 1350 1784 374 146 1280 Fragments arranged by size: 1784 1350 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2010 Size: 6136 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 2480 2635 2840 4199 4796 5583 Size: 393 367 481 155 205 1359 597 787 Cuts at: 5583 5851 1239 Size: 268 1524 Fragments arranged by size: 1524 1359 787 597 481 393 367 268 205 155 EcoNI CCTnn'n_nnAGG Cuts at: 2204 2663 3225 2204 Size: 459 562 5115 Fragments arranged by size: 5115 562 459 EcoO109I rG'GnC_Cy Cuts at: 2182 2209 2616 2640 2785 3139 3325 2182 Size: 27 407 24 145 354 186 4993 Fragments arranged by size: 4993 407 354 186 145 27 24 EcoRI G'AATT_C Cuts at: 3445 3823 3445 Size: 378 5758 FspI TGC'GCA Cuts at: 4642 4642 Size: 6136 HaeII r_GCGC'y Cuts at: 1678 2037 3270 3387 5283 1678 Size: 359 1233 117 1896 2531 Fragments arranged by size: 2531 1896 1233 359 117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2207 2239 2608 2640 4777 Size: 32 135 32 635 32 369 32 2137 Cuts at: 4777 4809 4851 4883 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2137 635 369 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3480 3726 669 Size: 988 281 1542 246 3079 Fragments arranged by size: 3079 1542 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6136 HpaI GTT'AAC Cuts at: 3726 3726 Size: 6136 KasI G'GCGC_C Cuts at: 2033 3383 2033 Size: 1350 4786 KpnI G_GTAC'C Cuts at: 3461 3461 Size: 6136 MfeI C'AATT_G Cuts at: 3735 3735 Size: 6136 MluI A'CGCG_T Cuts at: 6012 6012 Size: 6136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5250 5434 1305 Size: 3945 184 2007 Fragments arranged by size: 3945 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3398 10 Size: 54 1425 343 1566 2748 Fragments arranged by size: 2748 1566 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2066 2603 2834 2933 3287 4094 4453 Size: 648 900 537 231 99 354 807 359 Cuts at: 4453 4612 518 Size: 159 2042 Fragments arranged by size: 2042 900 807 648 537 359 354 231 159 99 MspA1I CmG'CkG Cuts at: 1606 2634 2894 2989 3100 3262 3346 3552 4229 Size: 1028 260 95 111 162 84 206 677 Cuts at: 4229 5377 5622 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1028 677 260 245 206 162 111 95 84 NarI GG'CG_CC Cuts at: 2034 3384 2034 Size: 1350 4786 NcoI C'CATG_G Cuts at: 513 1065 2415 3336 513 Size: 552 1350 921 3313 Fragments arranged by size: 3313 1350 921 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6136 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6136 NotI GC'GGCC_GC Cuts at: 3583 3583 Size: 6136 NspI r_CATG'y Cuts at: 1132 1229 3494 5039 1132 Size: 97 2265 1545 2229 Fragments arranged by size: 2265 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6136 PciI A'CATG_T Cuts at: 5035 5035 Size: 6136 PflMI CCAn_nnn'nTGG Cuts at: 1108 2212 2443 1108 Size: 1104 231 4801 Fragments arranged by size: 4801 1104 231 PfoI T'CCnGG_A Cuts at: 2199 2451 3135 2199 Size: 252 684 5200 Fragments arranged by size: 5200 684 252 PmeI GTTT'AAAC Cuts at: 3440 3440 Size: 6136 PmlI CAC'GTG Cuts at: 2362 2362 Size: 6136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4205 4237 5718 5750 4205 Size: 32 1481 32 4591 Fragments arranged by size: 4591 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2209 2616 3139 2209 Size: 407 523 5206 Fragments arranged by size: 5206 523 407 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6136 PsiI TTA'TAA Cuts at: 3706 3706 Size: 6136 PspOMI G'GGCC_C Cuts at: 2640 2718 2640 Size: 78 6058 PspXI vC'TCGA_Gb Cuts at: 3143 3143 Size: 6136 PstI C_TGCA'G Cuts at: 830 2166 3488 830 Size: 1336 1322 3478 Fragments arranged by size: 3478 1336 1322 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6136 PvuII CAG'CTG Cuts at: 2634 2989 3100 2634 Size: 355 111 5670 Fragments arranged by size: 5670 355 111 SacI G_AGCT'C Cuts at: 2012 2012 Size: 6136 SalI G'TCGA_C Cuts at: 1936 3478 1936 Size: 1542 4594 SanDI GG'GwC_CC Cuts at: 2616 2616 Size: 6136 SbfI CC_TGCA'GG Cuts at: 3488 3488 Size: 6136 ScaI AGT'ACT Cuts at: 4384 6096 4384 Size: 1712 4424 SfcI C'TryA_G Cuts at: 826 1045 2162 2539 2592 3484 4619 5300 5491 Size: 219 1117 377 53 892 1135 681 191 Cuts at: 5491 826 Size: 1471 Fragments arranged by size: 1471 1135 1117 892 681 377 219 191 53 SfiI GGCCn_nnn'nGGCC Cuts at: 2238 2238 Size: 6136 SfoI GGC'GCC Cuts at: 2035 3385 2035 Size: 1350 4786 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6136 SmaI CCC'GGG Cuts at: 1872 2187 2386 2779 3463 1872 Size: 315 199 393 684 4545 Fragments arranged by size: 4545 684 393 315 199 SmlI C'TyrA_G Cuts at: 820 1017 1943 3143 4240 5141 5403 820 Size: 197 926 1200 1097 901 262 1553 Fragments arranged by size: 1553 1200 1097 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3927 493 Size: 3434 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6136 SphI G_CATG'C Cuts at: 1132 3494 1132 Size: 2362 3774 SspI AAT'ATT Cuts at: 5 52 4060 5 Size: 47 4008 2081 Fragments arranged by size: 4008 2081 47 StuI AGG'CCT Cuts at: 1517 2232 1517 Size: 715 5421 StyI C'CwwG_G Cuts at: 513 1065 2415 2431 3336 3387 3575 513 Size: 552 1350 16 905 51 188 3074 Fragments arranged by size: 3074 1350 905 552 188 51 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2163 2308 2828 4163 4180 4333 4518 Size: 1255 583 145 520 1335 17 153 185 Cuts at: 4518 4963 325 Size: 445 1498 Fragments arranged by size: 1498 1335 1255 583 520 445 185 153 145 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4382 6094 96 Size: 274 80 33 51 1443 2405 1712 138 Fragments arranged by size: 2405 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6096 6128 6096 Size: 32 6104 Tth111I GACn'n_nGTC Cuts at: 1316 2864 2903 1316 Size: 1548 39 4549 Fragments arranged by size: 4549 1548 39 XbaI T'CTAG_A Cuts at: 3472 3472 Size: 6136 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2359 3343 2359 Size: 984 5152 XhoI C'TCGA_G Cuts at: 1943 3143 1943 Size: 1200 4936 XmaI C'CCGG_G Cuts at: 1870 2185 2384 2777 3461 1870 Size: 315 199 393 684 4545 Fragments arranged by size: 4545 684 393 315 199 XmnI GAAnn'nnTTC Cuts at: 4265 4265 Size: 6136 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3944 276 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PsrI RsrII SacII SapI SexAI SrfI SwaI