(Linear) (Six-base) MAPSORT of: KIEE5631.seq Check: 4405 from: 1 to: 1431 KIEE5631.orf With 182 enzymes: SgfI * December 5, 2014 13:03 .. AhdI GACnn_n'nnGTC Cuts at: 0 216 1431 Size: 216 1215 AleI CACnn'nnGTG Cuts at: 0 598 829 1431 Size: 598 231 602 Fragments arranged by size: 602 598 231 AlwNI CAG_nnn'CTG Cuts at: 0 207 629 1098 1228 1257 1431 Size: 207 422 469 130 29 174 Fragments arranged by size: 469 422 207 174 130 29 ApaI G_GGCC'C Cuts at: 0 639 717 1431 Size: 639 78 714 Fragments arranged by size: 714 639 78 ApaLI G'TGCA_C Cuts at: 0 1018 1431 Size: 1018 413 AvaI C'yCGr_G Cuts at: 0 180 379 772 1003 1138 1431 Size: 180 199 393 231 135 293 Fragments arranged by size: 393 293 231 199 180 135 BamHI G'GATC_C Cuts at: 0 1242 1431 Size: 1242 189 BanI G'GyrC_C Cuts at: 0 28 1088 1378 1431 Size: 28 1060 290 53 Fragments arranged by size: 1060 290 53 28 BanII G_rGCy'C Cuts at: 0 7 47 446 639 717 1431 Size: 7 40 399 193 78 714 Fragments arranged by size: 714 399 193 78 40 7 BbeI G_GCGC'C Cuts at: 0 32 1382 1431 Size: 32 1350 49 Fragments arranged by size: 1350 49 32 BbsI GAAGACnn'nnnn_ Cuts at: 0 484 1118 1431 Size: 484 634 313 Fragments arranged by size: 634 484 313 BglI GCCn_nnn'nGGC Cuts at: 0 233 817 1319 1431 Size: 233 584 502 112 Fragments arranged by size: 584 502 233 112 BlpI GC'TnA_GC Cuts at: 0 1258 1431 Size: 1258 173 Bme1580I G_kGCm'C Cuts at: 0 639 717 1022 1431 Size: 639 78 305 409 Fragments arranged by size: 639 409 305 78 BmgBI CAC'GTC Cuts at: 0 1284 1431 Size: 1284 147 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 630 835 1431 Size: 475 155 205 596 Fragments arranged by size: 596 475 205 155 Bpu10I CC'TnA_GC Cuts at: 0 421 1405 1431 Size: 421 984 26 Fragments arranged by size: 984 421 26 BsaI GGTCTCn'nnnn_ Cuts at: 0 192 1170 1408 1431 Size: 192 978 238 23 Fragments arranged by size: 978 238 192 23 BsaAI yAC'GTr Cuts at: 0 357 546 1431 Size: 357 189 885 Fragments arranged by size: 885 357 189 BsaHI Gr'CG_yC Cuts at: 0 29 1379 1431 Size: 29 1350 52 Fragments arranged by size: 1350 52 29 BsaWI w'CCGG_w Cuts at: 0 332 580 1431 Size: 332 248 851 Fragments arranged by size: 851 332 248 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 88 118 1309 1339 1431 Size: 88 30 1191 30 92 Fragments arranged by size: 1191 92 88 30 30 BseYI C'CCAG_C Cuts at: 0 622 692 762 1054 1431 Size: 622 70 70 292 377 Fragments arranged by size: 622 377 292 70 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1390 1431 Size: 1390 41 BsiEI CG_ry'CG Cuts at: 0 893 1431 Size: 893 538 BsiHKAI G_wGCw'C Cuts at: 0 7 344 1022 1309 1431 Size: 7 337 678 287 122 Fragments arranged by size: 678 337 287 122 7 Bsp1286I G_dGCh'C Cuts at: 0 7 47 344 446 639 717 1022 1309 Size: 7 40 297 102 193 78 305 287 Cuts at: 1309 1431 Size: 122 Fragments arranged by size: 305 297 287 193 122 102 78 40 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 107 1431 Size: 107 1324 BsrBI CCG'CTC Cuts at: 0 668 1431 Size: 668 763 BstAPI GCAn_nnn'nTGC Cuts at: 0 1095 1431 Size: 1095 336 BstEII G'GTnAC_C Cuts at: 0 840 1069 1431 Size: 840 229 362 Fragments arranged by size: 840 362 229 BstXI CCAn_nnnn'nTGG Cuts at: 0 831 1284 1431 Size: 831 453 147 Fragments arranged by size: 831 453 147 BstYI r'GATC_y Cuts at: 0 1242 1431 Size: 1242 189 BtgI C'CryG_G Cuts at: 0 251 410 599 953 1331 1431 Size: 251 159 189 354 378 100 Fragments arranged by size: 378 354 251 189 159 100 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 507 1431 Size: 507 924 BtsI GCAGTG_nn' Cuts at: 0 509 1244 1431 Size: 509 735 187 Fragments arranged by size: 735 509 187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 415 450 1431 Size: 415 35 981 Fragments arranged by size: 981 415 35 DraIII CAC_nnn'GTG Cuts at: 0 1017 1431 Size: 1017 414 DrdI GACnn_nn'nnGTC Cuts at: 0 457 547 1431 Size: 457 90 884 Fragments arranged by size: 884 457 90 EaeI y'GGCC_r Cuts at: 0 890 1343 1391 1431 Size: 890 453 48 40 Fragments arranged by size: 890 453 48 40 EagI C'GGCC_G Cuts at: 0 890 1431 Size: 890 541 EarI CTCTTCn'nnn_ Cuts at: 0 480 661 1431 Size: 480 181 770 Fragments arranged by size: 770 480 181 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 934 1431 Size: 934 497 EcoICRI GAG'CTC Cuts at: 0 5 1431 Size: 5 1426 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 630 835 1431 Size: 475 155 205 596 Fragments arranged by size: 596 475 205 155 EcoNI CCTnn'n_nnAGG Cuts at: 0 199 658 1220 1431 Size: 199 459 562 211 Fragments arranged by size: 562 459 211 199 EcoO109I rG'GnC_Cy Cuts at: 0 177 204 611 635 780 1134 1320 1431 Size: 177 27 407 24 145 354 186 111 Fragments arranged by size: 407 354 186 177 145 111 27 24 HaeII r_GCGC'y Cuts at: 0 32 1265 1382 1431 Size: 32 1233 117 49 Fragments arranged by size: 1233 117 49 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 202 234 603 635 1431 Size: 202 32 369 32 796 Fragments arranged by size: 796 369 202 32 32 KasI G'GCGC_C Cuts at: 0 28 1378 1431 Size: 28 1350 53 Fragments arranged by size: 1350 53 28 MscI TGG'CCA Cuts at: 0 1393 1431 Size: 1393 38 MslI CAynn'nnrTG Cuts at: 0 61 598 829 928 1282 1431 Size: 61 537 231 99 354 149 Fragments arranged by size: 537 354 231 149 99 61 MspA1I CmG'CkG Cuts at: 0 629 889 984 1095 1257 1341 1431 Size: 629 260 95 111 162 84 90 Fragments arranged by size: 629 260 162 111 95 90 84 NarI GG'CG_CC Cuts at: 0 29 1379 1431 Size: 29 1350 52 Fragments arranged by size: 1350 52 29 NcoI C'CATG_G Cuts at: 0 410 1331 1431 Size: 410 921 100 Fragments arranged by size: 921 410 100 PflMI CCAn_nnn'nTGG Cuts at: 0 207 438 1431 Size: 207 231 993 Fragments arranged by size: 993 231 207 PfoI T'CCnGG_A Cuts at: 0 194 446 1130 1431 Size: 194 252 684 301 Fragments arranged by size: 684 301 252 194 PmlI CAC'GTG Cuts at: 0 357 1431 Size: 357 1074 PpuMI rG'GwC_Cy Cuts at: 0 204 611 1134 1431 Size: 204 407 523 297 Fragments arranged by size: 523 407 297 204 PspOMI G'GGCC_C Cuts at: 0 635 713 1431 Size: 635 78 718 Fragments arranged by size: 718 635 78 PspXI vC'TCGA_Gb Cuts at: 0 1138 1431 Size: 1138 293 PstI C_TGCA'G Cuts at: 0 161 1431 Size: 161 1270 PvuII CAG'CTG Cuts at: 0 629 984 1095 1431 Size: 629 355 111 336 Fragments arranged by size: 629 355 336 111 SacI G_AGCT'C Cuts at: 0 7 1431 Size: 7 1424 SanDI GG'GwC_CC Cuts at: 0 611 1431 Size: 611 820 SfcI C'TryA_G Cuts at: 0 157 534 587 1431 Size: 157 377 53 844 Fragments arranged by size: 844 377 157 53 SfiI GGCCn_nnn'nGGCC Cuts at: 0 233 1431 Size: 233 1198 SfoI GGC'GCC Cuts at: 0 30 1380 1431 Size: 30 1350 51 Fragments arranged by size: 1350 51 30 SmaI CCC'GGG Cuts at: 0 182 381 774 1431 Size: 182 199 393 657 Fragments arranged by size: 657 393 199 182 SmlI C'TyrA_G Cuts at: 0 1138 1431 Size: 1138 293 StuI AGG'CCT Cuts at: 0 227 1431 Size: 227 1204 StyI C'CwwG_G Cuts at: 0 410 426 1331 1382 1431 Size: 410 16 905 51 49 Fragments arranged by size: 905 410 51 49 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 158 303 823 1431 Size: 158 145 520 608 Fragments arranged by size: 608 520 158 145 Tth111I GACn'n_nGTC Cuts at: 0 859 898 1431 Size: 859 39 533 Fragments arranged by size: 859 533 39 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 354 1338 1431 Size: 354 984 93 Fragments arranged by size: 984 354 93 XhoI C'TCGA_G Cuts at: 0 1138 1431 Size: 1138 293 XmaI C'CCGG_G Cuts at: 0 180 379 772 1431 Size: 180 199 393 659 Fragments arranged by size: 659 393 199 180 Enzymes that do cut: AhdI AleI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I NarI NcoI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI SanDI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII Tth111I XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI BplI BpuEI BsaBI BseRI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I ClaI DraI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XbaI XmnI ZraI