################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AccI AccIII AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BamHI BanII BbeI BbrPI BbsI BclI BfaI BfrI BglI BlnI BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmFI BsmI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I FbaI FseI FspI HincII HindII HpaI KasI KspI MaeI MamI MfeI MluI MroI MslI MunI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI TfiI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 487 GACGTC GACGT^C Acc65I 1612 GGTACC G^GTACC AclI 562 AACGTT AA^CGTT AlwNI 700 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 54 AGCGCT AGC^GCT Asp718I 1612 GGTACC G^GTACC AvaI 803 CCCGGG C^[CT]CG[GA]G BalI 788 TGGCCA TGG^CCA BcgI 877 CGATGCATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 407 GTATCC GTATCC(6/5) BglII 1599 AGATCT A^GATCT BmyI 885 GTGCCC G[AGT]GC[ACT]^C BsaAI 1627 TACGTG [CT]AC^GT[GA] BsmBI 485 GAGACG CGTCTC(1/5) BsoBI 803 CCCGGG C^[CT]CG[GA]G Bsp1286I 885 GTGCCC G[AGT]GC[ACT]^C Bsp1407I 1127 TGTACA T^GTACA BspMI 329 ACCTGC ACCTGC(4/8) BsrGI 1127 TGTACA T^GTACA DraII 1330 AGGACCC [GA]G^G[ACGT]CC[CT] EarI 841 GAAGAG CTCTTC(1/4) Eco47III 54 AGCGCT AGC^GCT EcoO109I 1330 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 505 GAATTC G^AATTC EcoRV 231 GATATC GAT^ATC FauI 1537 CCCGC CCCGC(4/6) HaeII 54 AGCGCT [GA]GCGC^[CT] HphI 1150 GGTGA GGTGA(8/7) KpnI 1612 GGTACC GGTAC^C Ksp632I 841 GAAGAG CTCTTC(1/4) MluNI 788 TGGCCA TGG^CCA MscI 788 TGGCCA TGG^CCA MspA1I 323 CAGCTG C[AC]G^C[GT]G NdeI 1350 CATATG CA^TATG PpuMI 1330 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1221 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 562 AACGTT AA^CGTT PstI 1747 CTGCAG CTGCA^G PvuII 323 CAGCTG CAG^CTG SexAI 98 ACCTGGT A^CC[AT]GGT SmaI 803 CCCGGG CCC^GGG SmlI 550 CTTGAG C^T[CT][GA]AG SphI 938 GCATGC GCATG^C SspBI 1127 TGTACA T^GTACA XcmI 458 CCACAGAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 803 CCCGGG C^CCGGG XmaI 803 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 505 GAATTC [GA]^AATT[CT] 1524 GAATTT AcyI 487 GACGTC G[GA]^CG[CT]C 1441 GACGCC ApoI 505 GAATTC [GA]^AATT[CT] 1524 GAATTT BanI 1612 GGTACC G^G[CT][GA]CC 1661 GGCACC BbvCI 28 CCTCAGC CCTCAGC(-5/-2) 4 GCTGAGG BlpI 57 GCTCAGC GC^T[ACGT]AGC 756 GCTCAGC BpmI 681 CTCCAG CTGGAG(16/14) 469 CTGGAG Bpu1102I 57 GCTCAGC GC^T[ACGT]AGC 756 GCTCAGC BsaHI 487 GACGTC G[GA]^CG[CT]C 1441 GACGCC BsgI 127 GTGCAG GTGCAG(16/14) 1709 GTGCAG BsrBI 754 CCGCTC CCGCTC(-3/-3) 1379 GAGCGG CelII 57 GCTCAGC GC^T[ACGT]AGC 756 GCTCAGC DraI 1495 TTTAAA TTT^AAA 1639 TTTAAA EaeI 149 CGGCCA [CT]^GGCC[GA] 788 TGGCCA EcoT22I 879 ATGCAT ATGCA^T 1530 ATGCAT HapII 124 CCGG C^CGG 804 CCGG Hin1I 487 GACGTC G[GA]^CG[CT]C 1441 GACGCC HpaII 124 CCGG C^CGG 804 CCGG MspI 124 CCGG C^CGG 804 CCGG NsiI 879 ATGCAT ATGCA^T 1530 ATGCAT NspI 881 GCATGT [GA]CATG^[CT] 938 GCATGC Tsp45I 413 GTGAC ^GT[GC]AC 826 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BbvI BcnI BsaJI BseRI BsiYI BslI BsmAI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HgaI HhaI HinP1I HindIII HinfI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp509I TspRI TthHB8I XhoII