(Circular) (Six-base) MAPSORT of: pFN21AE4157.seq Check: 9362 from: 1 to: 6496 pFN21AE4157 6496 bp With 182 enzymes: SgfI * March 28, 2012 13:10 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2496 4306 278 Size: 53 83 186 936 960 1810 2468 Fragments arranged by size: 2468 1810 960 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3839 1937 Size: 1902 4594 Acc65I G'GTAC_C Cuts at: 3617 3817 3617 Size: 200 6296 AclI AA'CG_TT Cuts at: 1563 2568 4623 4996 1563 Size: 1005 2055 373 3063 Fragments arranged by size: 3063 2055 1005 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2260 3743 4559 5943 6211 1632 Size: 628 1483 816 1384 268 1917 Fragments arranged by size: 1917 1483 1384 816 628 268 AfeI AGC'GCT Cuts at: 2061 2061 Size: 6496 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6299 AflIII A'CryG_T Cuts at: 1203 5395 6372 1203 Size: 4192 977 1327 Fragments arranged by size: 4192 1327 977 AgeI A'CCGG_T Cuts at: 5327 5327 Size: 6496 AhdI GACnn_n'nnGTC Cuts at: 5225 5225 Size: 6496 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6496 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3366 1184 Size: 2182 4314 AlwNI CAG_nnn'CTG Cuts at: 2710 4239 5811 2710 Size: 1529 1572 3395 Fragments arranged by size: 3395 1572 1529 ApaLI G'TGCA_C Cuts at: 4553 5709 4553 Size: 1156 5340 ApoI r'AATT_y Cuts at: 1493 2510 3529 3805 4031 4183 6292 1493 Size: 1017 1019 276 226 152 2109 1697 Fragments arranged by size: 2109 1697 1019 1017 276 226 152 AseI AT'TA_AT Cuts at: 160 1032 5050 160 Size: 872 4018 1606 Fragments arranged by size: 4018 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 AvaI C'yCGr_G Cuts at: 1870 1943 2808 3821 1870 Size: 73 865 1013 4545 Fragments arranged by size: 4545 1013 865 73 BamHI G'GATC_C Cuts at: 3826 4193 3826 Size: 367 6129 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3617 3666 3817 5272 618 Size: 325 217 627 1830 49 151 1455 1842 Fragments arranged by size: 1842 1830 1455 627 325 217 151 49 BanII G_rGCy'C Cuts at: 1391 3815 1391 Size: 2424 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5525 BbvCI CC'TCA_GC Cuts at: 2010 2034 2010 Size: 24 6472 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2871 2905 4687 4721 1903 Size: 34 934 34 1782 34 3678 Fragments arranged by size: 3678 1782 934 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2423 4388 5598 2423 Size: 1965 1210 3321 Fragments arranged by size: 3321 1965 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6496 BfrBI ATG'CAT Cuts at: 2886 3537 2886 Size: 651 5845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5107 136 Size: 107 122 71 631 605 3435 1525 Fragments arranged by size: 3435 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3604 6491 3604 Size: 2887 3609 BlpI GC'TnA_GC Cuts at: 2063 2762 3913 2063 Size: 699 1151 4646 Fragments arranged by size: 4646 1151 699 Bme1580I G_kGCm'C Cuts at: 1790 2894 4557 5713 1790 Size: 1104 1663 1156 2573 Fragments arranged by size: 2573 1663 1156 1104 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6496 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4227 5185 442 Size: 416 53 83 399 24 2810 958 1753 Fragments arranged by size: 2810 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6496 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2495 2671 5156 1239 Size: 1256 176 2485 2579 Fragments arranged by size: 2579 2485 1256 176 Bpu10I CC'TnA_GC Cuts at: 2010 2034 3031 2010 Size: 24 997 5475 Fragments arranged by size: 5475 997 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2576 4621 5486 5784 2576 Size: 2045 865 298 3288 Fragments arranged by size: 3288 2045 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5159 882 Size: 617 3660 2219 Fragments arranged by size: 3660 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3634 4287 493 Size: 711 2430 653 2702 Fragments arranged by size: 2702 2430 711 653 BsaBI GATnn'nnATC Cuts at: 6490 6490 Size: 6496 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2493 3447 4303 4685 Size: 53 83 186 936 960 954 856 382 Cuts at: 4685 5320 275 Size: 635 1451 Fragments arranged by size: 1451 960 954 936 856 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4929 5327 5601 5748 6159 4929 Size: 398 274 147 411 5266 Fragments arranged by size: 5266 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6271 Fragments arranged by size: 6271 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2135 3159 3388 1186 Size: 907 42 1024 229 4294 Fragments arranged by size: 4294 1024 907 229 42 BseYI C'CCAG_C Cuts at: 3339 5699 6195 3339 Size: 2360 496 3640 Fragments arranged by size: 3640 2360 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 3735 1163 Size: 573 417 1582 3924 Fragments arranged by size: 3924 1582 573 417 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3946 4707 5735 1540 Size: 275 186 1945 761 1028 2301 Fragments arranged by size: 2301 1945 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4557 4642 5713 4557 Size: 85 1071 5340 Fragments arranged by size: 5340 1071 85 BsmI GAATG_Cn' Cuts at: 4007 4100 4007 Size: 93 6403 BsmBI CGTCTCn'nnnn_ Cuts at: 2484 2484 Size: 6496 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2894 3815 4557 4642 5713 1391 Size: 399 1104 921 742 85 1071 2174 Fragments arranged by size: 2174 1104 1071 921 742 399 85 BspEI T'CCGG_A Cuts at: 6159 6159 Size: 6496 BspHI T'CATG_A Cuts at: 4385 6115 4385 Size: 1730 4766 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2343 3851 844 Size: 1499 1508 3489 Fragments arranged by size: 3489 1508 1499 BsrBI CCG'CTC Cuts at: 1394 1823 2761 3386 4383 1394 Size: 429 938 625 997 3507 Fragments arranged by size: 3507 997 938 625 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2388 2581 3071 3726 4991 5165 65 Size: 1183 1140 193 490 655 1265 174 1396 Fragments arranged by size: 1396 1265 1183 1140 655 490 193 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5140 5327 1700 Size: 110 3330 187 2869 Fragments arranged by size: 3330 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3132 96 Size: 3036 3460 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6496 BssSI C'ACGA_G Cuts at: 4556 5568 4556 Size: 1012 5484 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6496 BstBI TT'CG_AA Cuts at: 5300 5300 Size: 6496 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6496 BstYI r'GATC_y Cuts at: 1972 2124 2259 3510 3604 3826 4193 4578 4595 Size: 152 135 1251 94 222 367 385 17 Cuts at: 4595 6047 6133 6145 6491 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1251 385 367 346 222 152 135 94 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2959 3174 3228 3279 513 Size: 552 939 955 215 54 51 3730 Fragments arranged by size: 3730 955 939 552 215 54 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2801 2891 2896 487 Size: 683 56 1575 90 5 4087 Fragments arranged by size: 4087 1575 683 90 56 5 BtsI GCAGTG_nn' Cuts at: 2023 3350 4012 4806 4826 2023 Size: 1327 662 794 20 3693 Fragments arranged by size: 3693 1327 794 662 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6496 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6461 DraI TTT'AAA Cuts at: 3502 3646 3800 3502 Size: 144 154 6198 Fragments arranged by size: 6198 154 144 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5503 809 Size: 507 4187 1802 Fragments arranged by size: 4187 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2154 2793 3943 Size: 54 1425 50 275 18 324 639 1150 Cuts at: 3943 4832 6461 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1150 889 639 324 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3943 1537 Size: 275 2131 4090 Fragments arranged by size: 4090 2131 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2841 4426 1353 Size: 1488 1585 3423 Fragments arranged by size: 3423 1585 1488 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3737 5083 5457 5603 387 Size: 1064 138 2148 1346 374 146 1280 Fragments arranged by size: 2148 1346 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2260 2495 2671 3743 4559 5156 5943 Size: 393 628 235 176 1072 816 597 787 Cuts at: 5943 6211 1239 Size: 268 1524 Fragments arranged by size: 1524 1072 816 787 628 597 393 268 235 176 EcoO109I rG'GnC_Cy Cuts at: 3336 3336 Size: 6496 EcoRI G'AATT_C Cuts at: 2510 3805 4183 2510 Size: 1295 378 4823 Fragments arranged by size: 4823 1295 378 EcoRV GAT'ATC Cuts at: 2238 2238 Size: 6496 FspI TGC'GCA Cuts at: 5002 5002 Size: 6496 HaeII r_GCGC'y Cuts at: 1678 2063 5643 1678 Size: 385 3580 2531 Fragments arranged by size: 3580 2531 385 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2642 2674 2721 Size: 32 135 32 620 32 418 32 47 Cuts at: 2721 2753 5137 5169 5211 5243 1373 Size: 32 2384 32 42 32 2626 Fragments arranged by size: 2626 2384 620 418 135 47 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3840 4086 669 Size: 988 281 1902 246 3079 Fragments arranged by size: 3079 1902 988 281 246 HindIII A'AGCT_T Cuts at: 748 2534 3302 3740 748 Size: 1786 768 438 3504 Fragments arranged by size: 3504 1786 768 438 HpaI GTT'AAC Cuts at: 4086 4086 Size: 6496 KpnI G_GTAC'C Cuts at: 3621 3821 3621 Size: 200 6296 MfeI C'AATT_G Cuts at: 4095 4095 Size: 6496 MluI A'CGCG_T Cuts at: 6372 6372 Size: 6496 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2661 3717 5610 5794 1305 Size: 1356 1056 1893 184 2007 Fragments arranged by size: 2007 1893 1356 1056 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2795 10 Size: 54 1425 343 963 3711 Fragments arranged by size: 3711 1425 963 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4454 4813 4972 518 Size: 648 3288 359 159 2042 Fragments arranged by size: 3288 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2330 3912 4589 5737 5982 1606 Size: 724 1582 677 1148 245 2120 Fragments arranged by size: 2120 1582 1148 724 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2959 3174 3228 3279 513 Size: 552 939 955 215 54 51 3730 Fragments arranged by size: 3730 955 939 552 215 54 51 NdeI CA'TA_TG Cuts at: 387 3356 387 Size: 2969 3527 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6496 NotI GC'GGCC_GC Cuts at: 3943 3943 Size: 6496 NsiI A_TGCA'T Cuts at: 2888 3539 2888 Size: 651 5845 NspI r_CATG'y Cuts at: 1132 1229 2890 2947 3854 5399 1132 Size: 97 1661 57 907 1545 2229 Fragments arranged by size: 2229 1661 1545 907 97 57 PasI CC'CwG_GG Cuts at: 1348 3451 1348 Size: 2103 4393 PciI A'CATG_T Cuts at: 5395 5395 Size: 6496 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6496 PfoI T'CCnGG_A Cuts at: 2262 2262 Size: 6496 PmeI GTTT'AAAC Cuts at: 3800 3800 Size: 6496 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4565 4597 6078 6110 4565 Size: 32 1481 32 4951 Fragments arranged by size: 4951 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3336 3336 Size: 6496 PshAI GACnn'nnGTC Cuts at: 1613 3230 1613 Size: 1617 4879 PsiI TTA'TAA Cuts at: 2217 4066 2217 Size: 1849 4647 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2709 2741 2709 Size: 32 6464 PstI C_TGCA'G Cuts at: 830 3756 3848 830 Size: 2926 92 3478 Fragments arranged by size: 3478 2926 92 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6496 PvuII CAG'CTG Cuts at: 2330 2330 Size: 6496 SalI G'TCGA_C Cuts at: 1936 3838 1936 Size: 1902 4594 SbfI CC_TGCA'GG Cuts at: 3848 3848 Size: 6496 ScaI AGT'ACT Cuts at: 4744 6456 4744 Size: 1712 4784 SexAI A'CCwGG_T Cuts at: 2103 2103 Size: 6496 SfcI C'TryA_G Cuts at: 826 1045 3315 3576 3752 3844 4979 5660 5851 Size: 219 2270 261 176 92 1135 681 191 Cuts at: 5851 826 Size: 1471 Fragments arranged by size: 2270 1471 1135 681 261 219 191 176 92 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6496 SmaI CCC'GGG Cuts at: 1872 2810 3823 1872 Size: 938 1013 4545 Fragments arranged by size: 4545 1013 938 SmlI C'TyrA_G Cuts at: 820 1017 1943 2555 4600 5501 5763 820 Size: 197 926 612 2045 901 262 1553 Fragments arranged by size: 2045 1553 926 901 612 262 197 SnaBI TAC'GTA Cuts at: 493 4287 493 Size: 3794 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6496 SphI G_CATG'C Cuts at: 1132 2947 3854 1132 Size: 1815 907 3774 Fragments arranged by size: 3774 1815 907 SspI AAT'ATT Cuts at: 5 52 4420 5 Size: 47 4368 2081 Fragments arranged by size: 4368 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6496 StyI C'CwwG_G Cuts at: 513 1065 2004 2959 3174 3228 3279 3935 513 Size: 552 939 955 215 54 51 656 3074 Fragments arranged by size: 3074 955 939 656 552 215 54 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3272 4523 4540 4693 4878 5323 325 Size: 1255 1692 1251 17 153 185 445 1498 Fragments arranged by size: 1692 1498 1255 1251 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3132 4742 6454 Size: 274 80 33 51 1443 1155 1610 1712 Cuts at: 6454 96 Size: 138 Fragments arranged by size: 1712 1610 1443 1155 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6456 6488 6456 Size: 32 6464 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6496 XbaI T'CTAG_A Cuts at: 3832 3832 Size: 6496 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2470 2470 Size: 6496 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6496 XmaI C'CCGG_G Cuts at: 1870 2808 3821 1870 Size: 938 1013 4545 Fragments arranged by size: 4545 1013 938 XmnI GAAnn'nnTTC Cuts at: 4625 4625 Size: 6496 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2494 4304 276 Size: 53 83 186 936 960 1810 2468 Fragments arranged by size: 2468 1810 960 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI