################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BcgI BclI BcnI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsmBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HpaI HpaII KasI KpnI Ksp632I KspI MamI MluI MluNI MroI MscI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflII 601 CTTAAG C^TTAAG AlwNI 529 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 1002 CCCGAG C^[CT]CG[GA]G BamHI 950 GGATCC G^GATCC BbvCI 501 GCTGAGG CCTCAGC(-5/-2) BciVI 1047 GTATCC GTATCC(6/5) BfrI 601 CTTAAG C^TTAAG BsaAI 451 TACGTG [CT]AC^GT[GA] BsiWI 476 CGTACG C^GTACG BsmAI 7 GAGAC GTCTC(1/5) BsmI 384 GAATGC GAATGC(1/-1) BsoBI 1002 CCCGAG C^[CT]CG[GA]G BspHI 714 TCATGA T^CATGA BsrI 435 CCAGT ACTGG(1/-1) BstBI 398 TTCGAA TT^CGAA BstYI 950 GGATCC [GA]^GATC[CT] EcoT14I 678 CCTTGG C^C[AT][AT]GG HaeII 531 GGCGCT [GA]GCGC^[CT] HaeIII 78 GGCC GG^CC HincII 286 GTTGAC GT[CT]^[GA]AC HindII 286 GTTGAC GT[CT]^[GA]AC HindIII 808 AAGCTT A^AGCTT HphI 277 GGTGA GGTGA(8/7) MaeIII 355 GTGAC ^GT[ACGT]AC MfeI 539 CAATTG C^AATTG MflI 950 GGATCC [GA]^GATC[CT] MunI 539 CAATTG C^AATTG MwoI 928 GCACCAGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NheI 544 GCTAGC G^CTAGC NspV 398 TTCGAA TT^CGAA PstI 106 CTGCAG CTGCA^G PvuII 1008 CAGCTG CAG^CTG RcaI 714 TCATGA T^CATGA SexAI 273 ACCAGGT A^CC[AT]GGT SfuI 398 TTCGAA TT^CGAA StuI 77 AGGCCT AGG^CCT StyI 678 CCTTGG C^C[AT][AT]GG TaqI 399 TCGA T^CGA Tsp45I 355 GTGAC ^GT[GC]AC TspRI 189 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 399 TCGA T^CGA XhoII 950 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 517 CCGC CCGC(-3/-1) 934 GCGG BseRI 492 CTCCTC GAGGAG(10/8) 379 GAGGAG BsiYI 497 CCATGCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 517 CCGCTTCTGGG BslI 497 CCATGCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 517 CCGCTTCTGGG BsmFI 991 GGGAC GGGAC(10/14) 1039 GTCCC BstF5I 3 GGATG GGATG(2/0) 249 GGATG CfoI 507 GCGC GCG^C 532 GCGC DraII 991 GGGACCC [GA]G^G[ACGT]CC[CT] 998 AGGACCC EcoO109I 991 GGGACCC [GA]G^G[ACGT]CC[CT] 998 AGGACCC FokI 3 GGATG GGATG(9/13) 249 GGATG HhaI 507 GCGC GCG^C 532 GCGC HinP1I 507 GCGC G^CGC 532 GCGC MaeII 452 ACGT A^CGT 487 ACGT MslI 160 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 213 CATTTATATG MspA1I 932 CAGCGG C[AC]G^C[GT]G 1008 CAGCTG PpuMI 991 GGGACCC [GA]G^G[AT]CC[CT] 998 AGGACCC SmlI 601 CTTAAG C^T[CT][GA]AG 755 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AvaII BbvI BfaI BsaJI BssKI BstNI Cac8I Cfr13I DdeI DpnII DraI EcoRII Fnu4HI HinfI ItaI MaeI MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp509I