(Circular) (Six-base) MAPSORT of: pFN21AE9250.seq Check: 677 from: 1 to: 5764 pFN21AE9250 5764 bp With 182 enzymes: SgfI * March 28, 2012 12:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3574 278 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3107 1937 Size: 1170 4594 Acc65I G'GTAC_C Cuts at: 3085 3085 Size: 5764 AclI AA'CG_TT Cuts at: 1563 3891 4264 1563 Size: 2328 373 3063 Fragments arranged by size: 3063 2328 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2099 2324 2356 3827 5211 5479 1632 Size: 467 225 32 1471 1384 268 1917 Fragments arranged by size: 1917 1471 1384 467 268 225 32 AflII C'TTAA_G Cuts at: 820 1017 2606 820 Size: 197 1589 3978 Fragments arranged by size: 3978 1589 197 AflIII A'CryG_T Cuts at: 1203 4663 5640 1203 Size: 3460 977 1327 Fragments arranged by size: 3460 1327 977 AgeI A'CCGG_T Cuts at: 4595 4595 Size: 5764 AhdI GACnn_n'nnGTC Cuts at: 4493 4493 Size: 5764 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5764 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2965 1184 Size: 1781 3983 AlwNI CAG_nnn'CTG Cuts at: 2539 3507 5079 2539 Size: 968 1572 3224 Fragments arranged by size: 3224 1572 968 ApaLI G'TGCA_C Cuts at: 3821 4977 3821 Size: 1156 4608 ApoI r'AATT_y Cuts at: 1493 2122 2320 2334 2798 2825 3073 3299 3451 Size: 629 198 14 464 27 248 226 152 Cuts at: 3451 5560 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 629 464 248 226 198 152 27 14 AseI AT'TA_AT Cuts at: 160 1032 4318 160 Size: 872 3286 1606 Fragments arranged by size: 3286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 AvaI C'yCGr_G Cuts at: 1870 1943 3007 3089 1870 Size: 73 1064 82 4545 Fragments arranged by size: 4545 1064 82 73 BamHI G'GATC_C Cuts at: 2955 3094 3461 2955 Size: 139 367 5258 Fragments arranged by size: 5258 367 139 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3085 4540 618 Size: 325 217 627 1298 1455 1842 Fragments arranged by size: 1842 1455 1298 627 325 217 BanII G_rGCy'C Cuts at: 1391 3083 1391 Size: 1692 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4793 BbvCI CC'TCA_GC Cuts at: 2507 2507 Size: 5764 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3955 3989 1903 Size: 34 2018 34 3678 Fragments arranged by size: 3678 2018 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3063 3656 4866 3063 Size: 593 1210 3961 Fragments arranged by size: 3961 1210 593 BclI T'GATC_A Cuts at: 1548 1548 Size: 5764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4375 136 Size: 107 122 71 631 605 2703 1525 Fragments arranged by size: 2703 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5759 5759 Size: 5764 BlpI GC'TnA_GC Cuts at: 3181 3181 Size: 5764 Bme1580I G_kGCm'C Cuts at: 1790 3825 4981 1790 Size: 2035 1156 2573 Fragments arranged by size: 2573 2035 1156 BmgBI CAC'GTC Cuts at: 1318 2493 1318 Size: 1175 4589 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3495 4453 442 Size: 416 53 83 399 24 2078 958 1753 Fragments arranged by size: 2078 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2553 1056 Size: 1497 4267 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4424 1239 Size: 3185 2579 Bpu10I CC'TnA_GC Cuts at: 2507 2929 2507 Size: 422 5342 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2745 3889 4754 5052 2745 Size: 1144 865 298 3457 Fragments arranged by size: 3457 1144 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4427 882 Size: 617 2928 2219 Fragments arranged by size: 2928 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2458 3555 493 Size: 711 1254 1097 2702 Fragments arranged by size: 2702 1254 1097 711 BsaBI GATnn'nnATC Cuts at: 5758 5758 Size: 5764 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3571 3953 4588 275 Size: 53 83 186 936 2038 382 635 1451 Fragments arranged by size: 2038 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4197 4595 4869 5016 5427 4197 Size: 398 274 147 411 4534 Fragments arranged by size: 4534 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5539 Fragments arranged by size: 5539 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2399 2488 1186 Size: 1213 89 4462 Fragments arranged by size: 4462 1213 89 BseYI C'CCAG_C Cuts at: 4967 5463 4967 Size: 496 5268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5191 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3214 3975 5003 1540 Size: 275 186 1213 761 1028 2301 Fragments arranged by size: 2301 1213 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3825 3910 4981 3825 Size: 85 1071 4608 Fragments arranged by size: 4608 1071 85 BsiWI C'GTAC_G Cuts at: 2481 2481 Size: 5764 BsmI GAATG_Cn' Cuts at: 2395 3275 3368 2395 Size: 880 93 4791 Fragments arranged by size: 4791 880 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3083 3825 3910 4981 1391 Size: 399 1293 742 85 1071 2174 Fragments arranged by size: 2174 1293 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5427 5427 Size: 5764 BspHI T'CATG_A Cuts at: 2719 3653 5383 2719 Size: 934 1730 3100 Fragments arranged by size: 3100 1730 934 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3119 844 Size: 2275 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3651 1394 Size: 429 1828 3507 Fragments arranged by size: 3507 1828 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4259 4433 65 Size: 1183 3011 174 1396 Fragments arranged by size: 3011 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4408 4595 1700 Size: 110 2598 187 2869 Fragments arranged by size: 2869 2598 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5764 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5764 BssSI C'ACGA_G Cuts at: 3824 4836 3824 Size: 1012 4752 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5764 BstBI TT'CG_AA Cuts at: 2404 4568 2404 Size: 2164 3600 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5764 BstYI r'GATC_y Cuts at: 1972 2955 3094 3461 3846 3863 5315 5401 5413 Size: 983 139 367 385 17 1452 86 12 Cuts at: 5413 5759 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 983 385 367 346 139 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4273 Fragments arranged by size: 4273 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5025 Fragments arranged by size: 5025 683 56 BtsI GCAGTG_nn' Cuts at: 3280 4074 4094 3280 Size: 794 20 4950 Fragments arranged by size: 4950 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5729 DraI TTT'AAA Cuts at: 2317 2623 2841 3068 2317 Size: 306 218 227 5013 Fragments arranged by size: 5013 306 227 218 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4771 809 Size: 507 3455 1802 Fragments arranged by size: 3455 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3211 4100 5729 Size: 54 1425 50 275 18 1381 889 1629 Cuts at: 5729 8 Size: 43 Fragments arranged by size: 1629 1425 1381 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3211 1537 Size: 275 1399 4090 Fragments arranged by size: 4090 1399 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3694 1353 Size: 2341 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4351 4725 4871 387 Size: 1064 138 2762 374 146 1280 Fragments arranged by size: 2762 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2324 2356 3827 4424 5211 5479 Size: 393 467 225 32 1471 597 787 268 Cuts at: 5479 1239 Size: 1524 Fragments arranged by size: 1524 1471 787 597 467 393 268 225 32 EcoO109I rG'GnC_Cy Cuts at: 2997 3004 2997 Size: 7 5757 EcoRI G'AATT_C Cuts at: 3073 3451 3073 Size: 378 5386 FspI TGC'GCA Cuts at: 4270 4270 Size: 5764 HaeII r_GCGC'y Cuts at: 1678 2540 4911 1678 Size: 862 2371 2531 Fragments arranged by size: 2531 2371 862 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 4405 4437 4479 Size: 32 135 32 509 32 2292 32 42 Cuts at: 4479 4511 1373 Size: 32 2626 Fragments arranged by size: 2626 2292 509 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2293 3108 3354 669 Size: 988 281 355 815 246 3079 Fragments arranged by size: 3079 988 815 355 281 246 HindIII A'AGCT_T Cuts at: 748 2813 748 Size: 2065 3699 HpaI GTT'AAC Cuts at: 3354 3354 Size: 5764 KpnI G_GTAC'C Cuts at: 3089 3089 Size: 5764 MfeI C'AATT_G Cuts at: 2544 3363 2544 Size: 819 4945 MluI A'CGCG_T Cuts at: 5640 5640 Size: 5764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2152 2773 4878 5062 1305 Size: 847 621 2105 184 2007 Fragments arranged by size: 2105 2007 847 621 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3942 Fragments arranged by size: 3942 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2169 2222 3722 4081 4240 518 Size: 648 1003 53 1500 359 159 2042 Fragments arranged by size: 2042 1500 1003 648 359 159 53 MspA1I CmG'CkG Cuts at: 1606 2939 3015 3180 3857 5005 5250 1606 Size: 1333 76 165 677 1148 245 2120 Fragments arranged by size: 2120 1333 1148 677 245 165 76 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4273 Fragments arranged by size: 4273 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5764 NheI G'CTAG_C Cuts at: 1052 2549 1052 Size: 1497 4267 NotI GC'GGCC_GC Cuts at: 3211 3211 Size: 5764 NspI r_CATG'y Cuts at: 1132 1229 3122 4667 1132 Size: 97 1893 1545 2229 Fragments arranged by size: 2229 1893 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5764 PciI A'CATG_T Cuts at: 4663 4663 Size: 5764 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5764 PmeI GTTT'AAAC Cuts at: 3068 3068 Size: 5764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3833 3865 5346 5378 3833 Size: 32 1481 32 4219 Fragments arranged by size: 4219 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2997 3004 2997 Size: 7 5757 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5764 PsiI TTA'TAA Cuts at: 3334 3334 Size: 5764 PstI C_TGCA'G Cuts at: 830 2115 3116 830 Size: 1285 1001 3478 Fragments arranged by size: 3478 1285 1001 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5764 PvuII CAG'CTG Cuts at: 3015 3015 Size: 5764 SalI G'TCGA_C Cuts at: 1936 3106 1936 Size: 1170 4594 SanDI GG'GwC_CC Cuts at: 2997 2997 Size: 5764 SbfI CC_TGCA'GG Cuts at: 3116 3116 Size: 5764 ScaI AGT'ACT Cuts at: 4012 5724 4012 Size: 1712 4052 SexAI A'CCwGG_T Cuts at: 2278 2278 Size: 5764 SfcI C'TryA_G Cuts at: 826 1045 2111 2145 2669 3112 4247 4928 5119 Size: 219 1066 34 524 443 1135 681 191 Cuts at: 5119 826 Size: 1471 Fragments arranged by size: 1471 1135 1066 681 524 443 219 191 34 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5764 SmaI CCC'GGG Cuts at: 1872 3091 1872 Size: 1219 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2606 2760 3868 4769 5031 820 Size: 197 926 663 154 1108 901 262 1553 Fragments arranged by size: 1553 1108 926 901 663 262 197 154 SnaBI TAC'GTA Cuts at: 493 3555 493 Size: 3062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5764 SphI G_CATG'C Cuts at: 1132 3122 1132 Size: 1990 3774 SspI AAT'ATT Cuts at: 5 52 3688 5 Size: 47 3636 2081 Fragments arranged by size: 3636 2081 47 StuI AGG'CCT Cuts at: 1517 2084 1517 Size: 567 5197 StyI C'CwwG_G Cuts at: 513 1065 2004 2683 3203 513 Size: 552 939 679 520 3074 Fragments arranged by size: 3074 939 679 552 520 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3791 3808 3961 4146 4591 325 Size: 1255 2211 17 153 185 445 1498 Fragments arranged by size: 2211 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2424 2442 2463 Size: 274 80 33 51 1443 447 18 21 Cuts at: 2463 4010 5722 96 Size: 1547 1712 138 Fragments arranged by size: 1712 1547 1443 447 274 138 80 51 33 21 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5724 5756 5724 Size: 32 5732 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5764 XbaI T'CTAG_A Cuts at: 3100 3100 Size: 5764 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5764 XmaI C'CCGG_G Cuts at: 1870 3089 1870 Size: 1219 4545 XmnI GAAnn'nnTTC Cuts at: 3893 3893 Size: 5764 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3572 276 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI