################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XhoII XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AlwI 9 GGATC GGATC(4/5) AlwNI 769 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 591 GGACC G^G[AT]CC BbvCI 552 CCTCAGC CCTCAGC(-5/-2) BlpI 135 GCTGAGC GC^T[ACGT]AGC Bpu1102I 135 GCTGAGC GC^T[ACGT]AGC BsiWI 720 CGTACG C^GTACG BstF5I 21 CATCC GGATG(2/0) CelII 135 GCTGAGC GC^T[ACGT]AGC DpnII 10 GATC ^GATC EarI 610 GAAGAG CTCTTC(1/4) FokI 21 CATCC GGATG(9/13) HgaI 511 GACGC GACGC(5/10) Ksp632I 610 GAAGAG CTCTTC(1/4) KspI 24 CCGCGG CCGC^GG MboI 10 GATC ^GATC NdeII 10 GATC ^GATC NlaIII 645 CATG CATG^ PvuII 52 CAGCTG CAG^CTG SacII 24 CCGCGG CCGC^GG SapI 610 GAAGAGC GCTCTTC(1/4) Sau3AI 10 GATC ^GATC SexAI 533 ACCTGGT A^CC[AT]GGT SfaNI 20 GCATC GCATC(5/9) SgrAI 322 CGCCGGCG C[GA]^CCGG[CT]G SmaI 339 CCCGGG CCC^GGG XcmI 578 CCACCTGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 339 CCCGGG C^CCGGG XmaI 339 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### ApaI 201 GGGCCC GGGCC^C 568 GGGCCC DdeI 136 CTGAG C^T[ACGT]AG 553 CTCAG DraII 260 AGGCCCC [GA]G^G[ACGT]CC[CT] 567 GGGGCCC DsaI 24 CCGCGG C^C[GA][CT]GG 635 CCGTGG EcoO109I 260 AGGCCCC [GA]G^G[ACGT]CC[CT] 567 GGGGCCC EcoT14I 146 CCAAGG C^C[AT][AT]GG 179 CCAAGG HinfI 37 GAATC G^A[ACGT]TC 657 GAATC MboII 34 GAAGA GAAGA(8/7) 610 GAAGA MslI 177 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 645 CATGCCTGTG PstI 376 CTGCAG CTGCA^G 763 CTGCAG SfcI 376 CTGCAG C^T[GA][CT]AG 763 CTGCAG StyI 146 CCAAGG C^C[AT][AT]GG 179 CCAAGG TfiI 37 GAATC G^A[AT]TC 657 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaI BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsoBI Bsp1286I BsrFI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIV PaeR7I RsaI Sau96I ScrFI SfoI SmlI TaqI TseI TthHB8I XhoI