(Circular) (Six-base) MAPSORT of: pFN21AB9813.seq Check: 5228 from: 1 to: 5521 pFN21AB9813 5521 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3331 278 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2864 1937 Size: 927 4594 Acc65I G'GTAC_C Cuts at: 2842 2842 Size: 5521 AclI AA'CG_TT Cuts at: 1563 3648 4021 1563 Size: 2085 373 3063 Fragments arranged by size: 3063 2085 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2058 3584 4968 5236 1632 Size: 426 1526 1384 268 1917 Fragments arranged by size: 1917 1526 1384 426 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5324 AflIII A'CryG_T Cuts at: 1203 4420 5397 1203 Size: 3217 977 1327 Fragments arranged by size: 3217 1327 977 AgeI A'CCGG_T Cuts at: 4352 4352 Size: 5521 AhdI GACnn_n'nnGTC Cuts at: 4250 4250 Size: 5521 AleI CACnn'nnGTG Cuts at: 1166 2186 1166 Size: 1020 4501 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2204 1184 Size: 1020 4501 AlwNI CAG_nnn'CTG Cuts at: 2779 3264 4836 2779 Size: 485 1572 3464 Fragments arranged by size: 3464 1572 485 ApaI G_GGCC'C Cuts at: 2210 2577 2210 Size: 367 5154 ApaLI G'TGCA_C Cuts at: 3578 4734 3578 Size: 1156 4365 ApoI r'AATT_y Cuts at: 1493 2830 3056 3208 5317 1493 Size: 1337 226 152 2109 1697 Fragments arranged by size: 2109 1697 1337 226 152 AseI AT'TA_AT Cuts at: 160 1032 4075 160 Size: 872 3043 1606 Fragments arranged by size: 3043 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 AvaI C'yCGr_G Cuts at: 1870 1943 2344 2435 2609 2738 2846 1870 Size: 73 401 91 174 129 108 4545 Fragments arranged by size: 4545 401 174 129 108 91 73 BamHI G'GATC_C Cuts at: 2851 3218 2851 Size: 367 5154 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2083 2090 2180 2331 2804 Size: 325 217 627 296 7 90 151 473 Cuts at: 2804 2842 4297 618 Size: 38 1455 1842 Fragments arranged by size: 1842 1455 627 473 325 296 217 151 90 38 7 BanII G_rGCy'C Cuts at: 1391 2159 2210 2577 2840 1391 Size: 768 51 367 263 4072 Fragments arranged by size: 4072 768 367 263 51 BbeI G_GCGC'C Cuts at: 2087 2335 2808 2087 Size: 248 473 4800 Fragments arranged by size: 4800 473 248 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4550 BbvCI CC'TCA_GC Cuts at: 2558 2558 Size: 5521 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3712 3746 1903 Size: 34 50 34 1691 34 3678 Fragments arranged by size: 3678 1691 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3413 4623 3413 Size: 1210 4311 BclI T'GATC_A Cuts at: 1548 1548 Size: 5521 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2587 2742 2802 Size: 107 122 71 631 605 915 155 60 Cuts at: 2802 4132 136 Size: 1330 1525 Fragments arranged by size: 1525 1330 915 631 605 155 122 107 71 60 BglII A'GATC_T Cuts at: 5516 5516 Size: 5521 BlpI GC'TnA_GC Cuts at: 2141 2938 2141 Size: 797 4724 Bme1580I G_kGCm'C Cuts at: 1790 2210 2577 3582 4738 1790 Size: 420 367 1005 1156 2573 Fragments arranged by size: 2573 1156 1005 420 367 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5521 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3252 4210 442 Size: 416 53 83 399 24 1835 958 1753 Fragments arranged by size: 1835 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5521 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2414 2477 4181 1239 Size: 950 225 63 1704 2579 Fragments arranged by size: 2579 1704 950 225 63 Bpu10I CC'TnA_GC Cuts at: 2558 2558 Size: 5521 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3646 4511 4809 3646 Size: 865 298 4358 Fragments arranged by size: 4358 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4184 882 Size: 617 2685 2219 Fragments arranged by size: 2685 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3312 493 Size: 711 2108 2702 Fragments arranged by size: 2702 2108 711 BsaBI GATnn'nnATC Cuts at: 5515 5515 Size: 5521 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2084 2332 2805 3328 Size: 53 83 186 936 551 248 473 523 Cuts at: 3328 3710 4345 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 551 523 473 382 248 186 83 53 BsaWI w'CCGG_w Cuts at: 3954 4352 4626 4773 5184 3954 Size: 398 274 147 411 4291 Fragments arranged by size: 4291 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5296 Fragments arranged by size: 5296 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2093 2372 1186 Size: 904 3 279 4335 Fragments arranged by size: 4335 904 279 3 BseYI C'CCAG_C Cuts at: 2680 2712 2814 4724 5220 2680 Size: 32 102 1910 496 2981 Fragments arranged by size: 2981 1910 496 102 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4948 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2070 2250 2274 2287 2971 3732 Size: 275 186 69 180 24 13 684 761 Cuts at: 3732 4760 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 684 275 186 180 69 24 13 BsiHKAI G_wGCw'C Cuts at: 3582 3667 4738 3582 Size: 85 1071 4365 Fragments arranged by size: 4365 1071 85 BsiWI C'GTAC_G Cuts at: 2725 2725 Size: 5521 BsmI GAATG_Cn' Cuts at: 3032 3125 3032 Size: 93 5428 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2159 2210 2577 2840 3582 3667 4738 Size: 399 369 51 367 263 742 85 1071 Cuts at: 4738 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 369 367 263 85 51 BspEI T'CCGG_A Cuts at: 5184 5184 Size: 5521 BspHI T'CATG_A Cuts at: 3410 5140 3410 Size: 1730 3791 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2876 844 Size: 2032 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3408 1394 Size: 429 1585 3507 Fragments arranged by size: 3507 1585 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4016 4190 65 Size: 1183 2768 174 1396 Fragments arranged by size: 2768 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2328 2398 2630 4165 4352 1700 Size: 110 518 70 232 1535 187 2869 Fragments arranged by size: 2869 1535 518 232 187 110 70 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5521 BssHII G'CGCG_C Cuts at: 1925 2126 2128 2425 2427 2620 1925 Size: 201 2 297 2 193 4826 Fragments arranged by size: 4826 297 201 193 2 2 BssSI C'ACGA_G Cuts at: 3581 4593 3581 Size: 1012 4509 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5521 BstBI TT'CG_AA Cuts at: 4325 4325 Size: 5521 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5521 BstYI r'GATC_y Cuts at: 1972 2851 3218 3603 3620 5072 5158 5170 5516 Size: 879 367 385 17 1452 86 12 346 Cuts at: 5516 1972 Size: 1977 Fragments arranged by size: 1977 1452 879 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2029 2640 513 Size: 552 964 611 3394 Fragments arranged by size: 3394 964 611 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4782 Fragments arranged by size: 4782 683 56 BtsI GCAGTG_nn' Cuts at: 3037 3831 3851 3037 Size: 794 20 4707 Fragments arranged by size: 4707 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5521 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5486 DraI TTT'AAA Cuts at: 2825 2825 Size: 5521 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4528 809 Size: 507 3212 1802 Fragments arranged by size: 3212 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2247 2271 Size: 54 1425 50 275 18 237 180 24 Cuts at: 2271 2478 2511 2670 2968 3857 5486 8 Size: 207 33 159 298 889 1629 43 Fragments arranged by size: 1629 1425 889 298 275 237 207 180 159 54 50 43 33 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2067 2247 2271 2968 1537 Size: 275 255 180 24 697 4090 Fragments arranged by size: 4090 697 275 255 180 24 EarI CTCTTCn'nnn_ Cuts at: 1353 2610 3451 1353 Size: 1257 841 3423 Fragments arranged by size: 3423 1257 841 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2294 2732 4108 4482 4628 387 Size: 1064 138 705 438 1376 374 146 1280 Fragments arranged by size: 1376 1280 1064 705 438 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2189 2414 2477 3584 4181 4968 Size: 393 426 131 225 63 1107 597 787 Cuts at: 4968 5236 1239 Size: 268 1524 Fragments arranged by size: 1524 1107 787 597 426 393 268 225 131 63 EcoO109I rG'GnC_Cy Cuts at: 2266 2573 2266 Size: 307 5214 EcoRI G'AATT_C Cuts at: 2830 3208 2830 Size: 378 5143 FspI TGC'GCA Cuts at: 4027 4027 Size: 5521 HaeII r_GCGC'y Cuts at: 1678 2087 2335 2375 2381 2393 2450 2648 2755 Size: 409 248 40 6 12 57 198 107 Cuts at: 2755 2808 4668 1678 Size: 53 1860 2531 Fragments arranged by size: 2531 1860 409 248 198 107 57 53 40 12 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2453 2485 4162 4194 4236 Size: 32 135 32 881 32 1677 32 42 Cuts at: 4236 4268 1373 Size: 32 2626 Fragments arranged by size: 2626 1677 881 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2865 3111 669 Size: 988 281 927 246 3079 Fragments arranged by size: 3079 988 927 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5521 HpaI GTT'AAC Cuts at: 3111 3111 Size: 5521 KasI G'GCGC_C Cuts at: 2083 2331 2804 2083 Size: 248 473 4800 Fragments arranged by size: 4800 473 248 KpnI G_GTAC'C Cuts at: 2846 2846 Size: 5521 MfeI C'AATT_G Cuts at: 3120 3120 Size: 5521 MluI A'CGCG_T Cuts at: 5397 5397 Size: 5521 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4635 4819 1305 Size: 3330 184 2007 Fragments arranged by size: 3330 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3699 Fragments arranged by size: 3699 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 2654 3479 3838 3997 518 Size: 648 1020 468 825 359 159 2042 Fragments arranged by size: 2042 1020 825 648 468 359 159 MspA1I CmG'CkG Cuts at: 1606 2031 2059 2688 2937 3614 4762 5007 1606 Size: 425 28 629 249 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 629 425 249 245 28 NaeI GCC'GGC Cuts at: 2330 2400 2632 2330 Size: 70 232 5219 Fragments arranged by size: 5219 232 70 NarI GG'CG_CC Cuts at: 2084 2332 2805 2084 Size: 248 473 4800 Fragments arranged by size: 4800 473 248 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4969 NdeI CA'TA_TG Cuts at: 387 387 Size: 5521 NgoMIV G'CCGG_C Cuts at: 2328 2398 2630 2328 Size: 70 232 5219 Fragments arranged by size: 5219 232 70 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5521 NotI GC'GGCC_GC Cuts at: 2968 2968 Size: 5521 NspI r_CATG'y Cuts at: 1132 1229 2879 4424 1132 Size: 97 1650 1545 2229 Fragments arranged by size: 2229 1650 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5521 PciI A'CATG_T Cuts at: 4420 4420 Size: 5521 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5521 PfoI T'CCnGG_A Cuts at: 2592 2592 Size: 5521 PmeI GTTT'AAAC Cuts at: 2825 2825 Size: 5521 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3590 3622 5103 5135 3590 Size: 32 1481 32 3976 Fragments arranged by size: 3976 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5521 PsiI TTA'TAA Cuts at: 3091 3091 Size: 5521 PspOMI G'GGCC_C Cuts at: 2206 2573 2206 Size: 367 5154 PspXI vC'TCGA_Gb Cuts at: 2435 2609 2738 2435 Size: 174 129 5218 Fragments arranged by size: 5218 174 129 PstI C_TGCA'G Cuts at: 830 2385 2772 2873 830 Size: 1555 387 101 3478 Fragments arranged by size: 3478 1555 387 101 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5521 PvuII CAG'CTG Cuts at: 2059 2059 Size: 5521 SacII CC_GC'GG Cuts at: 2032 2032 Size: 5521 SalI G'TCGA_C Cuts at: 1936 2863 1936 Size: 927 4594 SapI GCTCTTCn'nnn_ Cuts at: 2610 2610 Size: 5521 SbfI CC_TGCA'GG Cuts at: 2873 2873 Size: 5521 ScaI AGT'ACT Cuts at: 3769 5481 3769 Size: 1712 3809 SexAI A'CCwGG_T Cuts at: 2538 2538 Size: 5521 SfcI C'TryA_G Cuts at: 826 1045 2381 2768 2869 4004 4685 4876 826 Size: 219 1336 387 101 1135 681 191 1471 Fragments arranged by size: 1471 1336 1135 681 387 219 191 101 SfoI GGC'GCC Cuts at: 2085 2333 2806 2085 Size: 248 473 4800 Fragments arranged by size: 4800 473 248 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 SgrAI Cr'CCGG_yG Cuts at: 1700 2328 1700 Size: 628 4893 SmaI CCC'GGG Cuts at: 1872 2346 2848 1872 Size: 474 502 4545 Fragments arranged by size: 4545 502 474 SmlI C'TyrA_G Cuts at: 820 1017 1943 2435 2609 2738 3625 4526 4788 Size: 197 926 492 174 129 887 901 262 Cuts at: 4788 820 Size: 1553 Fragments arranged by size: 1553 926 901 887 492 262 197 174 129 SnaBI TAC'GTA Cuts at: 493 3312 493 Size: 2819 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5521 SphI G_CATG'C Cuts at: 1132 2879 1132 Size: 1747 3774 SspI AAT'ATT Cuts at: 5 52 3445 5 Size: 47 3393 2081 Fragments arranged by size: 3393 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5521 StyI C'CwwG_G Cuts at: 513 1065 2151 2184 2960 513 Size: 552 1086 33 776 3074 Fragments arranged by size: 3074 1086 776 552 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3548 3565 3718 3903 4348 325 Size: 1255 1968 17 153 185 445 1498 Fragments arranged by size: 1968 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3767 5479 96 Size: 274 80 33 51 1443 1790 1712 138 Fragments arranged by size: 1790 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5481 5513 5481 Size: 32 5489 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5521 XbaI T'CTAG_A Cuts at: 2857 2857 Size: 5521 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2590 2590 Size: 5521 XhoI C'TCGA_G Cuts at: 1943 2435 2609 2738 1943 Size: 492 174 129 4726 Fragments arranged by size: 4726 492 174 129 XmaI C'CCGG_G Cuts at: 1870 2344 2846 1870 Size: 474 502 4545 Fragments arranged by size: 4545 502 474 XmnI GAAnn'nnTTC Cuts at: 3650 3650 Size: 5521 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3329 276 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PsrI RsrII SacI SanDI SfiI SrfI SwaI