################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII KpnI KspI MaeI MaeII MaeIII MamI MfeI MluI MroI MunI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tsp45I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Alw44I 890 GTGCAC G^TGCAC ApaLI 890 GTGCAC G^TGCAC Asp700I 1282 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 879 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 716 TGGCCA TGG^CCA BanI 86 GGCGCC G^G[CT][GA]CC BbeI 86 GGCGCC GGCGC^C BbsI 1312 GAAGAC GAAGAC(2/6) BglI 718 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 370 GCTGAGC GC^T[ACGT]AGC Bpu1102I 370 GCTGAGC GC^T[ACGT]AGC BpuAI 1312 GAAGAC GAAGAC(2/6) BsgI 1384 GTGCAG GTGCAG(16/14) BsrFI 212 GCCGGT [GA]^CCGG[CT] CelII 370 GCTGAGC GC^T[ACGT]AGC Cfr10I 212 GCCGGT [GA]^CCGG[CT] DraIII 283 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 716 TGGCCA [CT]^GGCC[GA] HincII 1438 GTTAAC GT[CT]^[GA]AC HindII 1438 GTTAAC GT[CT]^[GA]AC HpaI 1438 GTTAAC GTT^AAC KasI 86 GGCGCC G^GCGCC MluNI 716 TGGCCA TGG^CCA MscI 716 TGGCCA TGG^CCA NarI 86 GGCGCC GG^CGCC PflFI 879 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 551 AGGACCC [GA]G^G[AT]CC[CT] PstI 1048 CTGCAG CTGCA^G SacI 373 GAGCTC GAGCT^C ScaI 1108 AGTACT AGT^ACT SfoI 86 GGCGCC GGC^GCC SspI 589 AATATT AAT^ATT StuI 1125 AGGCCT AGG^CCT Tth111I 879 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 1282 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### ApaI 74 GGGCCC GGGCC^C 108 GGGCCC BsaI 643 GAGACC GGTCTC(1/5) 987 GAGACC BssSI 91 CACGAG CACGAG(-5/-1) 184 CACGAG BstYI 233 AGATCC [GA]^GATC[CT] 933 GGATCT EarI 145 GAAGAG CTCTTC(1/4) 1273 GAAGAG EcoT14I 269 CCTTGG C^C[AT][AT]GG 548 CCAAGG HgaI 691 GACGC GACGC(5/10) 55 GCGTC HphI 1030 GGTGA GGTGA(8/7) 967 TCACC Ksp632I 145 GAAGAG CTCTTC(1/4) 1273 GAAGAG MflI 233 AGATCC [GA]^GATC[CT] 933 GGATCT MslI 682 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 846 CATTGGAATG NdeI 541 CATATG CA^TATG 638 CATATG PvuII 41 CAGCTG CAG^CTG 1448 CAGCTG SfaNI 660 GATGC GCATC(5/9) 964 GCATC SfcI 1048 CTGCAG C^T[GA][CT]AG 1352 CTGTAG StyI 269 CCTTGG C^C[AT][AT]GG 548 CCAAGG TaqI 449 TCGA T^CGA 597 TCGA TthHB8I 449 TCGA T^CGA 597 TCGA XcmI 455 CCAGTAGAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1185 CCAACCCCTCCCTGG XhoII 233 AGATCC [GA]^GATC[CT] 933 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SmlI TfiI Tru9I TseI Tsp509I TspRI