(Circular) (Six-base) MAPSORT of: pFN21AE1153.seq Check: 9189 from: 1 to: 6205 pFN21AE1153 6205 bp With 182 enzymes: SgfI * October 29, 2012 18:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3548 1937 Size: 1611 4594 Acc65I G'GTAC_C Cuts at: 3526 3526 Size: 6205 AclI AA'CG_TT Cuts at: 1563 3504 4332 4705 1563 Size: 1941 828 373 3063 Fragments arranged by size: 3063 1941 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2215 3141 4268 5652 5920 1632 Size: 583 926 1127 1384 268 1917 Fragments arranged by size: 1917 1384 1127 926 583 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6008 AflIII A'CryG_T Cuts at: 1203 5104 6081 1203 Size: 3901 977 1327 Fragments arranged by size: 3901 1327 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 4934 4934 Size: 6205 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6205 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6205 AlwNI CAG_nnn'CTG Cuts at: 2323 2575 2795 3948 5520 2323 Size: 252 220 1153 1572 3008 Fragments arranged by size: 3008 1572 1153 252 220 ApaI G_GGCC'C Cuts at: 2083 2117 2083 Size: 34 6171 ApaLI G'TGCA_C Cuts at: 2895 4262 5418 2895 Size: 1367 1156 3682 Fragments arranged by size: 3682 1367 1156 ApoI r'AATT_y Cuts at: 1493 2347 2429 2513 2532 3514 3740 3892 6001 Size: 854 82 84 19 982 226 152 2109 Cuts at: 6001 1493 Size: 1697 Fragments arranged by size: 2109 1697 982 854 226 152 84 82 19 AseI AT'TA_AT Cuts at: 160 1032 4759 160 Size: 872 3727 1606 Fragments arranged by size: 3727 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 3530 1870 Size: 73 1587 4545 Fragments arranged by size: 4545 1587 73 BamHI G'GATC_C Cuts at: 3535 3902 3535 Size: 367 5838 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2091 3526 4981 618 Size: 325 217 627 304 1435 1455 1842 Fragments arranged by size: 1842 1455 1435 627 325 304 217 BanII G_rGCy'C Cuts at: 1391 2083 2117 2382 2706 3123 3524 1391 Size: 692 34 265 324 417 401 4072 Fragments arranged by size: 4072 692 417 401 324 265 34 BbeI G_GCGC'C Cuts at: 2095 2095 Size: 6205 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3324 928 Size: 971 1425 3809 Fragments arranged by size: 3809 1425 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4396 4430 1903 Size: 34 2459 34 3678 Fragments arranged by size: 3678 2459 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4097 5307 4097 Size: 1210 4995 BclI T'GATC_A Cuts at: 1548 1548 Size: 6205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2729 4816 136 Size: 107 122 71 631 605 1057 2087 1525 Fragments arranged by size: 2087 1525 1057 631 605 122 107 71 BglII A'GATC_T Cuts at: 6200 6200 Size: 6205 BlpI GC'TnA_GC Cuts at: 2376 3622 2376 Size: 1246 4959 Bme1580I G_kGCm'C Cuts at: 1790 2083 2117 2899 4266 5422 1790 Size: 293 34 782 1367 1156 2573 Fragments arranged by size: 2573 1367 1156 782 293 34 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3421 3936 4894 Size: 416 53 83 399 24 2004 515 958 Cuts at: 4894 442 Size: 1753 Fragments arranged by size: 2004 1753 958 515 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6205 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3061 3061 Size: 6205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2432 2699 3222 3405 4865 1239 Size: 1193 267 523 183 1460 2579 Fragments arranged by size: 2579 1460 1193 523 267 183 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2159 2740 2940 4330 5195 5493 2159 Size: 581 200 1390 865 298 2871 Fragments arranged by size: 2871 1390 865 581 298 200 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2642 2986 4868 882 Size: 617 1143 344 1882 2219 Fragments arranged by size: 2219 1882 1143 617 344 BsaAI yAC'GTr Cuts at: 493 1204 3996 493 Size: 711 2792 2702 Fragments arranged by size: 2792 2702 711 BsaBI GATnn'nnATC Cuts at: 6199 6199 Size: 6205 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2060 2092 2697 4012 Size: 53 83 186 936 527 32 605 1315 Cuts at: 4012 4394 5029 275 Size: 382 635 1451 Fragments arranged by size: 1451 1315 936 635 605 527 382 186 83 53 32 BsaWI w'CCGG_w Cuts at: 4638 5036 5310 5457 5868 4638 Size: 398 274 147 411 4975 Fragments arranged by size: 4975 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5980 Fragments arranged by size: 5980 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2028 2702 3113 3130 1186 Size: 842 674 411 17 4261 Fragments arranged by size: 4261 842 674 411 17 BseYI C'CCAG_C Cuts at: 2107 2166 2965 3122 5408 5904 2107 Size: 59 799 157 2286 496 2408 Fragments arranged by size: 2408 2286 799 496 157 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3410 1163 Size: 573 1674 3958 Fragments arranged by size: 3958 1674 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3655 4416 5444 1540 Size: 275 186 1654 761 1028 2301 Fragments arranged by size: 2301 1654 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2175 2289 2382 2899 4266 4351 5422 2175 Size: 114 93 517 1367 85 1071 2958 Fragments arranged by size: 2958 1367 1071 517 114 93 85 BsmI GAATG_Cn' Cuts at: 3716 3809 3716 Size: 93 6112 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2117 2175 2289 2382 2706 2899 Size: 399 293 34 58 114 93 324 193 Cuts at: 2899 3123 3524 4266 4351 5422 1391 Size: 224 401 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 401 399 324 293 224 193 114 93 85 58 34 BspEI T'CCGG_A Cuts at: 5868 5868 Size: 6205 BspHI T'CATG_A Cuts at: 4094 5824 4094 Size: 1730 4475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3560 844 Size: 2716 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4092 1394 Size: 429 2269 3507 Fragments arranged by size: 3507 2269 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4700 4874 65 Size: 1183 3452 174 1396 Fragments arranged by size: 3452 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2217 4849 5036 1700 Size: 110 407 2632 187 2869 Fragments arranged by size: 2869 2632 407 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6205 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6205 BssSI C'ACGA_G Cuts at: 2096 2189 4265 5277 2096 Size: 93 2076 1012 3024 Fragments arranged by size: 3024 2076 1012 93 BstAPI GCAn_nnn'nTGC Cuts at: 794 2260 2665 2918 794 Size: 1466 405 253 4081 Fragments arranged by size: 4081 1466 405 253 BstBI TT'CG_AA Cuts at: 5009 5009 Size: 6205 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6205 BstYI r'GATC_y Cuts at: 1972 2238 2938 3535 3902 4287 4304 5756 5842 Size: 266 700 597 367 385 17 1452 86 Cuts at: 5842 5854 6200 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 700 597 385 367 346 266 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4714 Fragments arranged by size: 4714 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5466 Fragments arranged by size: 5466 683 56 BtsI GCAGTG_nn' Cuts at: 2324 2454 3721 4515 4535 2324 Size: 130 1267 794 20 3994 Fragments arranged by size: 3994 1267 794 130 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6205 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 3509 3509 Size: 6205 DraIII CAC_nnn'GTG Cuts at: 2293 2293 Size: 6205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5212 809 Size: 507 3896 1802 Fragments arranged by size: 3896 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2721 3652 4541 Size: 54 1425 50 275 18 891 931 889 Cuts at: 4541 6170 8 Size: 1629 43 Fragments arranged by size: 1629 1425 931 891 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3652 1537 Size: 275 1840 4090 Fragments arranged by size: 4090 1840 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2145 3273 4135 1353 Size: 792 1128 862 3423 Fragments arranged by size: 3423 1128 862 792 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2135 3493 4792 5166 5312 387 Size: 1064 138 546 1358 1299 374 146 1280 Fragments arranged by size: 1358 1299 1280 1064 546 374 146 138 EcoICRI GAG'CTC Cuts at: 2380 2380 Size: 6205 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2215 2432 2699 3141 3222 3405 4268 Size: 393 583 217 267 442 81 183 863 Cuts at: 4268 4865 5652 5920 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 863 787 597 583 442 393 268 267 217 183 81 EcoO109I rG'GnC_Cy Cuts at: 2113 2271 2557 2792 2113 Size: 158 286 235 5526 Fragments arranged by size: 5526 286 235 158 EcoRI G'AATT_C Cuts at: 3514 3892 3514 Size: 378 5827 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2200 2200 Size: 6205 FspI TGC'GCA Cuts at: 4711 4711 Size: 6205 HaeII r_GCGC'y Cuts at: 1678 2095 2198 3164 5352 1678 Size: 417 103 966 2188 2531 Fragments arranged by size: 2531 2188 966 417 103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2569 2601 3454 3486 4846 Size: 32 135 32 997 32 853 32 1360 Cuts at: 4846 4878 4920 4952 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1360 997 853 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3445 3549 3795 669 Size: 988 281 1507 104 246 3079 Fragments arranged by size: 3079 1507 988 281 246 104 HindIII A'AGCT_T Cuts at: 748 748 Size: 6205 HpaI GTT'AAC Cuts at: 3445 3795 3445 Size: 350 5855 KasI G'GCGC_C Cuts at: 2091 2091 Size: 6205 KpnI G_GTAC'C Cuts at: 3530 3530 Size: 6205 MfeI C'AATT_G Cuts at: 3804 3804 Size: 6205 MluI A'CGCG_T Cuts at: 6081 6081 Size: 6205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2867 5319 5503 1305 Size: 1562 2452 184 2007 Fragments arranged by size: 2452 2007 1562 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2723 10 Size: 54 1425 343 891 3492 Fragments arranged by size: 3492 1425 891 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2691 2855 4163 4522 4681 518 Size: 648 1525 164 1308 359 159 2042 Fragments arranged by size: 2042 1525 1308 648 359 164 159 MspA1I CmG'CkG Cuts at: 1606 2037 2048 2750 3455 3621 4298 5446 5691 Size: 431 11 702 705 166 677 1148 245 Cuts at: 5691 1606 Size: 2120 Fragments arranged by size: 2120 1148 705 702 677 431 245 166 11 NarI GG'CG_CC Cuts at: 2092 2092 Size: 6205 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4714 Fragments arranged by size: 4714 939 552 NdeI CA'TA_TG Cuts at: 387 2547 2644 387 Size: 2160 97 3948 Fragments arranged by size: 3948 2160 97 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6205 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 3563 5108 1132 Size: 97 2334 1545 2229 Fragments arranged by size: 2334 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6205 PciI A'CATG_T Cuts at: 5104 5104 Size: 6205 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6205 PfoI T'CCnGG_A Cuts at: 2241 2676 2241 Size: 435 5770 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4274 4306 5787 5819 4274 Size: 32 1481 32 4660 Fragments arranged by size: 4660 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2557 2557 Size: 6205 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6205 PsiI TTA'TAA Cuts at: 3775 3775 Size: 6205 PspOMI G'GGCC_C Cuts at: 2079 2113 2079 Size: 34 6171 PstI C_TGCA'G Cuts at: 830 3057 3557 830 Size: 2227 500 3478 Fragments arranged by size: 3478 2227 500 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6205 PvuII CAG'CTG Cuts at: 2048 3455 2048 Size: 1407 4798 SacI G_AGCT'C Cuts at: 2382 2382 Size: 6205 SalI G'TCGA_C Cuts at: 1936 3547 1936 Size: 1611 4594 SbfI CC_TGCA'GG Cuts at: 3557 3557 Size: 6205 ScaI AGT'ACT Cuts at: 3115 4453 6165 3115 Size: 1338 1712 3155 Fragments arranged by size: 3155 1712 1338 SfcI C'TryA_G Cuts at: 826 1045 3053 3357 3553 4688 5369 5560 826 Size: 219 2008 304 196 1135 681 191 1471 Fragments arranged by size: 2008 1471 1135 681 304 219 196 191 SfoI GGC'GCC Cuts at: 2093 2093 Size: 6205 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 3532 1872 Size: 1660 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2174 2755 2955 4309 5210 5472 Size: 197 926 231 581 200 1354 901 262 Cuts at: 5472 820 Size: 1553 Fragments arranged by size: 1553 1354 926 901 581 262 231 200 197 SnaBI TAC'GTA Cuts at: 493 3996 493 Size: 3503 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 3563 1132 Size: 2431 3774 SspI AAT'ATT Cuts at: 5 52 2596 4129 5 Size: 47 2544 1533 2081 Fragments arranged by size: 2544 2081 1533 47 StuI AGG'CCT Cuts at: 1517 3132 1517 Size: 1615 4590 StyI C'CwwG_G Cuts at: 513 1065 2004 2274 2553 3644 513 Size: 552 939 270 279 1091 3074 Fragments arranged by size: 3074 1091 939 552 279 270 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2158 2824 3262 4232 4249 4402 4587 Size: 1255 578 666 438 970 17 153 185 Cuts at: 4587 5032 325 Size: 445 1498 Fragments arranged by size: 1498 1255 970 666 578 445 438 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2759 3113 3323 Size: 274 80 33 51 1443 782 354 210 Cuts at: 3323 4451 6163 96 Size: 1128 1712 138 Fragments arranged by size: 1712 1443 1128 782 354 274 210 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2279 2311 6165 6197 2279 Size: 32 3854 32 2287 Fragments arranged by size: 3854 2287 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2887 1316 Size: 1571 4634 XbaI T'CTAG_A Cuts at: 3541 3541 Size: 6205 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2467 3197 2467 Size: 730 5475 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 3530 1870 Size: 1660 4545 XmnI GAAnn'nnTTC Cuts at: 3291 4334 3291 Size: 1043 5162 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI