(Linear) (Six-base) MAPSORT of: KIEE1153.seq Check: 8696 from: 1 to: 1500 KIEE1153.orf With 182 enzymes: SgfI * October 29, 2012 16:51 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 210 1136 1500 Size: 210 926 364 Fragments arranged by size: 926 364 210 AlwNI CAG_nnn'CTG Cuts at: 0 318 570 790 1500 Size: 318 252 220 710 Fragments arranged by size: 710 318 252 220 ApaI G_GGCC'C Cuts at: 0 78 112 1500 Size: 78 34 1388 Fragments arranged by size: 1388 78 34 ApaLI G'TGCA_C Cuts at: 0 890 1500 Size: 890 610 ApoI r'AATT_y Cuts at: 0 342 424 508 527 1500 Size: 342 82 84 19 973 Fragments arranged by size: 973 342 84 82 19 BanI G'GyrC_C Cuts at: 0 86 1500 Size: 86 1414 BanII G_rGCy'C Cuts at: 0 78 112 377 701 1118 1500 Size: 78 34 265 324 417 382 Fragments arranged by size: 417 382 324 265 78 34 BbeI G_GCGC'C Cuts at: 0 90 1500 Size: 90 1410 BbsI GAAGACnn'nnnn_ Cuts at: 0 1319 1500 Size: 1319 181 BglI GCCn_nnn'nGGC Cuts at: 0 724 1500 Size: 724 776 BlpI GC'TnA_GC Cuts at: 0 371 1500 Size: 371 1129 Bme1580I G_kGCm'C Cuts at: 0 78 112 894 1500 Size: 78 34 782 606 Fragments arranged by size: 782 606 78 34 BmrI ACTGGGnnnn_n' Cuts at: 0 1416 1500 Size: 1416 84 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1056 1500 Size: 1056 444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 427 694 1217 1400 1500 Size: 427 267 523 183 100 Fragments arranged by size: 523 427 267 183 100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 735 935 1500 Size: 154 581 200 565 Fragments arranged by size: 581 565 200 154 BsaI GGTCTCn'nnnn_ Cuts at: 0 637 981 1500 Size: 637 344 519 Fragments arranged by size: 637 519 344 BsaHI Gr'CG_yC Cuts at: 0 55 87 692 1500 Size: 55 32 605 808 Fragments arranged by size: 808 605 55 32 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 23 697 1108 1125 1500 Size: 23 674 411 17 375 Fragments arranged by size: 674 411 375 23 17 BseYI C'CCAG_C Cuts at: 0 102 161 960 1117 1500 Size: 102 59 799 157 383 Fragments arranged by size: 799 383 157 102 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1405 1500 Size: 1405 95 BsiHKAI G_wGCw'C Cuts at: 0 170 284 377 894 1500 Size: 170 114 93 517 606 Fragments arranged by size: 606 517 170 114 93 Bsp1286I G_dGCh'C Cuts at: 0 78 112 170 284 377 701 894 1118 Size: 78 34 58 114 93 324 193 224 Cuts at: 1118 1500 Size: 382 Fragments arranged by size: 382 324 224 193 114 93 78 58 34 BsrFI r'CCGG_y Cuts at: 0 212 1500 Size: 212 1288 BssSI C'ACGA_G Cuts at: 0 91 184 1500 Size: 91 93 1316 Fragments arranged by size: 1316 93 91 BstAPI GCAn_nnn'nTGC Cuts at: 0 255 660 913 1500 Size: 255 405 253 587 Fragments arranged by size: 587 405 255 253 BstYI r'GATC_y Cuts at: 0 233 933 1500 Size: 233 700 567 Fragments arranged by size: 700 567 233 BtsI GCAGTG_nn' Cuts at: 0 319 449 1500 Size: 319 130 1051 Fragments arranged by size: 1051 319 130 DraIII CAC_nnn'GTG Cuts at: 0 288 1500 Size: 288 1212 EaeI y'GGCC_r Cuts at: 0 716 1500 Size: 716 784 EarI CTCTTCn'nnn_ Cuts at: 0 140 1268 1500 Size: 140 1128 232 Fragments arranged by size: 1128 232 140 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 130 1488 1500 Size: 130 1358 12 Fragments arranged by size: 1358 130 12 EcoICRI GAG'CTC Cuts at: 0 375 1500 Size: 375 1125 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 210 427 694 1136 1217 1400 1500 Size: 210 217 267 442 81 183 100 Fragments arranged by size: 442 267 217 210 183 100 81 EcoO109I rG'GnC_Cy Cuts at: 0 108 266 552 787 1500 Size: 108 158 286 235 713 Fragments arranged by size: 713 286 235 158 108 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 195 1500 Size: 195 1305 HaeII r_GCGC'y Cuts at: 0 90 193 1159 1500 Size: 90 103 966 341 Fragments arranged by size: 966 341 103 90 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 564 596 1449 1481 1500 Size: 564 32 853 32 19 Fragments arranged by size: 853 564 32 32 19 HincII GTy'rAC Cuts at: 0 1440 1500 Size: 1440 60 HpaI GTT'AAC Cuts at: 0 1440 1500 Size: 1440 60 KasI G'GCGC_C Cuts at: 0 86 1500 Size: 86 1414 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 862 1500 Size: 862 638 MscI TGG'CCA Cuts at: 0 718 1500 Size: 718 782 MslI CAynn'nnrTG Cuts at: 0 686 850 1500 Size: 686 164 650 Fragments arranged by size: 686 650 164 MspA1I CmG'CkG Cuts at: 0 32 43 745 1450 1500 Size: 32 11 702 705 50 Fragments arranged by size: 705 702 50 32 11 NarI GG'CG_CC Cuts at: 0 87 1500 Size: 87 1413 NdeI CA'TA_TG Cuts at: 0 542 639 1500 Size: 542 97 861 Fragments arranged by size: 861 542 97 PfoI T'CCnGG_A Cuts at: 0 236 671 1500 Size: 236 435 829 Fragments arranged by size: 829 435 236 PpuMI rG'GwC_Cy Cuts at: 0 552 1500 Size: 552 948 PspOMI G'GGCC_C Cuts at: 0 74 108 1500 Size: 74 34 1392 Fragments arranged by size: 1392 74 34 PstI C_TGCA'G Cuts at: 0 1052 1500 Size: 1052 448 PvuII CAG'CTG Cuts at: 0 43 1450 1500 Size: 43 1407 50 Fragments arranged by size: 1407 50 43 SacI G_AGCT'C Cuts at: 0 377 1500 Size: 377 1123 ScaI AGT'ACT Cuts at: 0 1110 1500 Size: 1110 390 SfcI C'TryA_G Cuts at: 0 1048 1352 1500 Size: 1048 304 148 Fragments arranged by size: 1048 304 148 SfoI GGC'GCC Cuts at: 0 88 1500 Size: 88 1412 SmlI C'TyrA_G Cuts at: 0 169 750 950 1500 Size: 169 581 200 550 Fragments arranged by size: 581 550 200 169 SspI AAT'ATT Cuts at: 0 591 1500 Size: 591 909 StuI AGG'CCT Cuts at: 0 1127 1500 Size: 1127 373 StyI C'CwwG_G Cuts at: 0 269 548 1500 Size: 269 279 952 Fragments arranged by size: 952 279 269 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 153 819 1257 1500 Size: 153 666 438 243 Fragments arranged by size: 666 438 243 153 TatI w'GTAC_w Cuts at: 0 754 1108 1318 1500 Size: 754 354 210 182 Fragments arranged by size: 754 354 210 182 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 274 306 1500 Size: 274 32 1194 Fragments arranged by size: 1194 274 32 Tth111I GACn'n_nGTC Cuts at: 0 882 1500 Size: 882 618 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 462 1192 1500 Size: 462 730 308 Fragments arranged by size: 730 462 308 XmnI GAAnn'nnTTC Cuts at: 0 1286 1500 Size: 1286 214 Enzymes that do cut: AcuI AlwNI ApaI ApaLI ApoI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrFI BssSI BstAPI BstYI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII HpaI KasI MmeI MscI MslI MspA1I NarI NdeI PfoI PpuMI PspOMI PstI PvuII SacI ScaI SfcI SfoI SmlI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI Bpu10I BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EagI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII KpnI MfeI MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI XbaI XhoI XmaI ZraI