Restriction map for
pFN21AE2535
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AvrII BalI
BbrPI BbsI BbvCI BciVI BclI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmFI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI Ksp632I MamI MfeI MluI
MluNI MroI MscI MseI MunI
NciI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI ScaI
SfuI SmaI SnaBI SpeI SspI
StuI StyI SwaI Tru9I Tsp509I
Van91I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AlwNI 223 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 196 GAGCAC G[AT]GC[AT]^C
AspI 769 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 302 TGCGCA TGC^GCA
BanII 252 GGGCTC G[GA]GC[CT]^C
BbeI 145 GGCGCC GGCGC^C
BcgI 17 CGACAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 833 CTAG C^TAG
BseRI 697 GAGGAG GAGGAG(10/8)
BsiHKAI 196 GAGCAC G[AT]GC[AT]^C
BsmAI 484 GAGAC GTCTC(1/5)
BsmBI 484 GAGACG CGTCTC(1/5)
Bsp1407I 590 TGTACA T^GTACA
BspMI 164 ACCTGC ACCTGC(4/8)
BsrDI 270 CATTGC GCAATG(2/0)
BsrGI 590 TGTACA T^GTACA
BsrI 99 CCAGT ACTGG(1/-1)
DraIII 409 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 244 CCGCGG C^C[GA][CT]GG
EaeI 219 CGGCCA [CT]^GGCC[GA]
FspI 302 TGCGCA TGC^GCA
HaeII 145 GGCGCC [GA]GCGC^[CT]
HgaI 385 GACGC GACGC(5/10)
KasI 145 GGCGCC G^GCGCC
KspI 244 CCGCGG CCGC^GG
MaeI 833 CTAG C^TAG
MaeIII 88 GTGAC ^GT[ACGT]AC
MboII 404 TCTTC GAAGA(8/7)
NarI 145 GGCGCC GG^CGCC
PflFI 769 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 287 AGGACCC [GA]G^G[AT]CC[CT]
PstI 499 CTGCAG CTGCA^G
PvuII 10 CAGCTG CAG^CTG
SacII 244 CCGCGG CCGC^GG
SbfI 498 CCTGCAGG CCTGCA^GG
SexAI 770 ACCAGGT A^CC[AT]GGT
SfcI 499 CTGCAG C^T[GA][CT]AG
SfiI 597 GGCCTTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 145 GGCGCC GGC^GCC
SgrAI 199 CACCGGCG C[GA]^CCGG[CT]G
SmlI 346 CTCAAG C^T[CT][GA]AG
SphI 206 GCATGC GCATG^C
Sse8387I 498 CCTGCAGG CCTGCA^GG
SspBI 590 TGTACA T^GTACA
TfiI 616 GATTC G^A[AT]TC
Tsp45I 88 GTGAC ^GT[GC]AC
Tth111I 769 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 832 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 145 GGCGCC G[GA]^CG[CT]C
385 GACGCC
BamHI 660 GGATCC G^GATCC
702 GGATCC
BpmI 193 CTGGAG CTGGAG(16/14)
694 CTGGAG
BsaHI 145 GGCGCC G[GA]^CG[CT]C
385 GACGCC
BssSI 409 CACGAG CACGAG(-5/-1)
549 CTCGTG
BstYI 660 GGATCC [GA]^GATC[CT]
702 GGATCC
DpnII 661 GATC ^GATC
703 GATC
EcoNI 498 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
693 CCTGGAGGAGG
Hin1I 145 GGCGCC G[GA]^CG[CT]C
385 GACGCC
HphI 481 GGTGA GGTGA(8/7)
816 TCACC
MaeII 213 ACGT A^CGT
827 ACGT
MboI 661 GATC ^GATC
703 GATC
MflI 660 GGATCC [GA]^GATC[CT]
702 GGATCC
MspA1I 10 CAGCTG C[AC]G^C[GT]G
244 CCGCGG
NaeI 217 GCCGGC GCC^GGC
436 GCCGGC
NdeII 661 GATC ^GATC
703 GATC
NgoMIV 217 GCCGGC G^CCGGC
436 GCCGGC
NspI 206 GCATGC [GA]CATG^[CT]
392 ACATGC
Sau3AI 661 GATC ^GATC
703 GATC
TaqI 677 TCGA T^CGA
687 TCGA
TthHB8I 677 TCGA T^CGA
687 TCGA
XhoII 660 GGATCC [GA]^GATC[CT]
702 GGATCC
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanI BbvI BglI BmyI
BsaJI BsgI BsiYI BslI Bsp1286I
BsrFI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MnlI MslI MspI
MvaI MvnI MwoI NlaIII NlaIV
PleI RsaI Sau96I ScrFI SfaNI
TseI TspRI