(Circular) (Six-base) MAPSORT of: pFN21AE2535.seq Check: 7021 from: 1 to: 5548 pFN21AE2535 5548 bp With 182 enzymes: SgfI * July 15, 2014 17:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2178 2178 Size: 5548 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3358 278 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2891 1937 Size: 954 4594 Acc65I G'GTAC_C Cuts at: 2869 2869 Size: 5548 AclI AA'CG_TT Cuts at: 1563 3675 4048 1563 Size: 2112 373 3063 Fragments arranged by size: 3063 2112 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2255 3611 4995 5263 1632 Size: 623 1356 1384 268 1917 Fragments arranged by size: 1917 1384 1356 623 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5351 AflIII A'CryG_T Cuts at: 1203 4447 5424 1203 Size: 3244 977 1327 Fragments arranged by size: 3244 1327 977 AgeI A'CCGG_T Cuts at: 4379 4379 Size: 5548 AhdI GACnn_n'nnGTC Cuts at: 4277 4277 Size: 5548 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5548 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2333 1184 Size: 1149 4399 AlwNI CAG_nnn'CTG Cuts at: 2233 3291 4863 2233 Size: 1058 1572 2918 Fragments arranged by size: 2918 1572 1058 ApaLI G'TGCA_C Cuts at: 3605 4761 3605 Size: 1156 4392 ApoI r'AATT_y Cuts at: 1493 2857 3083 3235 5344 1493 Size: 1364 226 152 2109 1697 Fragments arranged by size: 2109 1697 1364 226 152 AseI AT'TA_AT Cuts at: 160 1032 4102 160 Size: 872 3070 1606 Fragments arranged by size: 3070 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 AvaI C'yCGr_G Cuts at: 1870 1943 2873 1870 Size: 73 930 4545 Fragments arranged by size: 4545 930 73 BamHI G'GATC_C Cuts at: 2665 2707 2878 3245 2665 Size: 42 171 367 4968 Fragments arranged by size: 4968 367 171 42 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2150 2429 2869 4324 Size: 325 217 627 255 108 279 440 1455 Cuts at: 4324 618 Size: 1842 Fragments arranged by size: 1842 1455 627 440 325 279 255 217 108 BanII G_rGCy'C Cuts at: 1391 2261 2867 1391 Size: 870 606 4072 Fragments arranged by size: 4072 870 606 BbeI G_GCGC'C Cuts at: 2154 2154 Size: 5548 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4577 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2011 2021 2045 3739 3773 1903 Size: 34 50 24 10 24 1694 34 3678 Fragments arranged by size: 3678 1694 50 34 34 24 24 10 BciVI GTATCCnnnnn_n' Cuts at: 3440 4650 3440 Size: 1210 4338 BclI T'GATC_A Cuts at: 1548 1548 Size: 5548 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2148 2228 2609 Size: 107 122 71 631 605 476 80 381 Cuts at: 2609 4159 136 Size: 1550 1525 Fragments arranged by size: 1550 1525 631 605 476 381 122 107 80 71 BglII A'GATC_T Cuts at: 5543 5543 Size: 5548 BlpI GC'TnA_GC Cuts at: 2965 2965 Size: 5548 Bme1580I G_kGCm'C Cuts at: 1790 2045 3609 4765 1790 Size: 255 1564 1156 2573 Fragments arranged by size: 2573 1564 1156 255 BmgBI CAC'GTC Cuts at: 1318 2219 1318 Size: 901 4647 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3279 4237 442 Size: 416 53 83 399 24 1862 958 1753 Fragments arranged by size: 1862 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2720 4208 1239 Size: 980 501 1488 2579 Fragments arranged by size: 2579 1488 980 501 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2336 3673 4538 4836 2336 Size: 1337 865 298 3048 Fragments arranged by size: 3048 1337 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4211 882 Size: 617 2712 2219 Fragments arranged by size: 2712 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3339 493 Size: 711 2135 2702 Fragments arranged by size: 2702 2135 711 BsaBI GATnn'nnATC Cuts at: 5542 5542 Size: 5548 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2151 2391 3355 3737 Size: 53 83 186 936 618 240 964 382 Cuts at: 3737 4372 275 Size: 635 1451 Fragments arranged by size: 1451 964 936 635 618 382 240 186 83 53 BsaWI w'CCGG_w Cuts at: 3981 4379 4653 4800 5211 3981 Size: 398 274 147 411 4318 Fragments arranged by size: 4318 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5323 Fragments arranged by size: 5323 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2717 1186 Size: 1531 4017 BseYI C'CCAG_C Cuts at: 2068 2328 2422 4751 5247 2068 Size: 260 94 2329 496 2369 Fragments arranged by size: 2369 2329 496 260 94 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2033 2150 2659 1163 Size: 573 297 117 509 4052 Fragments arranged by size: 4052 573 509 297 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2998 3759 4787 1540 Size: 275 186 997 761 1028 2301 Fragments arranged by size: 2301 1028 997 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2205 3609 3694 4765 2205 Size: 1404 85 1071 2988 Fragments arranged by size: 2988 1404 1071 85 BsmI GAATG_Cn' Cuts at: 3059 3152 3059 Size: 93 5455 BsmBI CGTCTCn'nnnn_ Cuts at: 2483 2483 Size: 5548 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2205 2261 2867 3609 3694 4765 Size: 399 255 160 56 606 742 85 1071 Cuts at: 4765 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 606 399 255 160 85 56 BspEI T'CCGG_A Cuts at: 5211 5211 Size: 5548 BspHI T'CATG_A Cuts at: 3437 5167 3437 Size: 1730 3818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2178 2836 2903 844 Size: 1334 658 67 3489 Fragments arranged by size: 3489 1334 658 67 BsrBI CCG'CTC Cuts at: 1394 1823 3435 1394 Size: 429 1612 3507 Fragments arranged by size: 3507 1612 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2274 4043 4217 65 Size: 1183 1026 1769 174 1396 Fragments arranged by size: 1769 1396 1183 1026 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 2205 2222 2441 4192 4379 1700 Size: 110 198 197 17 219 1751 187 2869 Fragments arranged by size: 2869 1751 219 198 197 187 110 17 BsrGI T'GTAC_A Cuts at: 96 2595 96 Size: 2499 3049 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5548 BssSI C'ACGA_G Cuts at: 2414 2554 3608 4620 2414 Size: 140 1054 1012 3342 Fragments arranged by size: 3342 1054 1012 140 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5548 BstBI TT'CG_AA Cuts at: 4352 4352 Size: 5548 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5548 BstYI r'GATC_y Cuts at: 1972 2665 2707 2878 3245 3630 3647 5099 5185 Size: 693 42 171 367 385 17 1452 86 Cuts at: 5185 5197 5543 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 693 385 367 346 171 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2249 513 Size: 552 1184 3812 Fragments arranged by size: 3812 1184 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4809 Fragments arranged by size: 4809 683 56 BtsI GCAGTG_nn' Cuts at: 2070 3064 3858 3878 2070 Size: 994 794 20 3740 Fragments arranged by size: 3740 994 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5548 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5513 DraI TTT'AAA Cuts at: 2852 2852 Size: 5548 DraIII CAC_nnn'GTG Cuts at: 2419 2419 Size: 5548 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4555 809 Size: 507 3239 1802 Fragments arranged by size: 3239 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2224 2995 3884 Size: 54 1425 50 275 18 394 771 889 Cuts at: 3884 5513 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 771 394 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2995 1537 Size: 275 1183 4090 Fragments arranged by size: 4090 1183 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3478 1353 Size: 2125 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4135 4509 4655 387 Size: 1064 138 2546 374 146 1280 Fragments arranged by size: 2546 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2255 2720 3611 4208 4995 5263 Size: 393 587 36 465 891 597 787 268 Cuts at: 5263 1239 Size: 1524 Fragments arranged by size: 1524 891 787 597 587 465 393 268 36 EcoNI CCTnn'n_nnAGG Cuts at: 2507 2702 2507 Size: 195 5353 EcoO109I rG'GnC_Cy Cuts at: 2230 2293 2611 2230 Size: 63 318 5167 Fragments arranged by size: 5167 318 63 EcoRI G'AATT_C Cuts at: 2857 3235 2857 Size: 378 5170 FspI TGC'GCA Cuts at: 2309 4054 2309 Size: 1745 3803 HaeII r_GCGC'y Cuts at: 1678 2154 4695 1678 Size: 476 2541 2531 Fragments arranged by size: 2541 2531 476 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373 Size: 32 135 32 2617 32 42 32 2626 Fragments arranged by size: 2626 2617 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2892 3138 669 Size: 988 281 954 246 3079 Fragments arranged by size: 3079 988 954 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5548 HpaI GTT'AAC Cuts at: 3138 3138 Size: 5548 KasI G'GCGC_C Cuts at: 2150 2150 Size: 5548 KpnI G_GTAC'C Cuts at: 2873 2873 Size: 5548 MfeI C'AATT_G Cuts at: 3147 3147 Size: 5548 MluI A'CGCG_T Cuts at: 5424 5424 Size: 5548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4662 4846 1305 Size: 3357 184 2007 Fragments arranged by size: 3357 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3726 Fragments arranged by size: 3726 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2366 2639 2684 3506 3865 4024 Size: 648 843 357 273 45 822 359 159 Cuts at: 4024 518 Size: 2042 Fragments arranged by size: 2042 843 822 648 359 357 273 159 45 MspA1I CmG'CkG Cuts at: 1606 2017 2251 2964 3641 4789 5034 1606 Size: 411 234 713 677 1148 245 2120 Fragments arranged by size: 2120 1148 713 677 411 245 234 NaeI GCC'GGC Cuts at: 2224 2443 2224 Size: 219 5329 NarI GG'CG_CC Cuts at: 2151 2151 Size: 5548 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4996 NdeI CA'TA_TG Cuts at: 387 387 Size: 5548 NgoMIV G'CCGG_C Cuts at: 2222 2441 2222 Size: 219 5329 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5548 NotI GC'GGCC_GC Cuts at: 2995 2995 Size: 5548 NspI r_CATG'y Cuts at: 1132 1229 2215 2401 2906 4451 1132 Size: 97 986 186 505 1545 2229 Fragments arranged by size: 2229 1545 986 505 186 97 PasI CC'CwG_GG Cuts at: 1348 2747 1348 Size: 1399 4149 PciI A'CATG_T Cuts at: 4447 4447 Size: 5548 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5548 PfoI T'CCnGG_A Cuts at: 2196 2697 2196 Size: 501 5047 PmeI GTTT'AAAC Cuts at: 2852 2852 Size: 5548 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3617 3649 5130 5162 3617 Size: 32 1481 32 4003 Fragments arranged by size: 4003 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2293 2293 Size: 5548 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5548 PsiI TTA'TAA Cuts at: 3118 3118 Size: 5548 PstI C_TGCA'G Cuts at: 830 2508 2900 830 Size: 1678 392 3478 Fragments arranged by size: 3478 1678 392 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5548 PvuII CAG'CTG Cuts at: 2017 2017 Size: 5548 SacII CC_GC'GG Cuts at: 2252 2252 Size: 5548 SalI G'TCGA_C Cuts at: 1936 2890 1936 Size: 954 4594 SbfI CC_TGCA'GG Cuts at: 2508 2900 2508 Size: 392 5156 ScaI AGT'ACT Cuts at: 3796 5508 3796 Size: 1712 3836 SexAI A'CCwGG_T Cuts at: 2775 2775 Size: 5548 SfcI C'TryA_G Cuts at: 826 1045 2504 2896 4031 4712 4903 826 Size: 219 1459 392 1135 681 191 1471 Fragments arranged by size: 1471 1459 1135 681 392 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2609 2609 Size: 5548 SfoI GGC'GCC Cuts at: 2152 2152 Size: 5548 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 SgrAI Cr'CCGG_yG Cuts at: 1700 2205 1700 Size: 505 5043 SmaI CCC'GGG Cuts at: 1872 2875 1872 Size: 1003 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2351 3652 4553 4815 820 Size: 197 926 408 1301 901 262 1553 Fragments arranged by size: 1553 1301 926 901 408 262 197 SnaBI TAC'GTA Cuts at: 493 3339 493 Size: 2846 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5548 SphI G_CATG'C Cuts at: 1132 2215 2906 1132 Size: 1083 691 3774 Fragments arranged by size: 3774 1083 691 SspI AAT'ATT Cuts at: 5 52 3472 5 Size: 47 3420 2081 Fragments arranged by size: 3420 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5548 StyI C'CwwG_G Cuts at: 513 1065 2987 513 Size: 552 1922 3074 Fragments arranged by size: 3074 1922 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 3575 3592 3745 3930 4375 325 Size: 1255 480 1515 17 153 185 445 1498 Fragments arranged by size: 1515 1498 1255 480 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2595 3794 5506 Size: 274 80 33 51 1443 618 1199 1712 Cuts at: 5506 96 Size: 138 Fragments arranged by size: 1712 1443 1199 618 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2117 2149 5508 5540 2117 Size: 32 3359 32 2125 Fragments arranged by size: 3359 2125 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2777 1316 Size: 1461 4087 XbaI T'CTAG_A Cuts at: 2837 2884 2837 Size: 47 5501 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5548 XmaI C'CCGG_G Cuts at: 1870 2873 1870 Size: 1003 4545 XmnI GAAnn'nnTTC Cuts at: 3677 3677 Size: 5548 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3356 276 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SrfI SwaI XcmI