Restriction map for
pFN21AE2535
(Linear) (Six-base) MAPSORT of: KIEE2535.seq Check: 636 from: 1 to: 843
KIEE2535.orf
With 182 enzymes: SgfI *
July 15, 2014 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 173 843
Size: 173 670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 843
Size: 250 593
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 328 843
Size: 328 515
AlwNI CAG_nnn'CTG
Cuts at: 0 228 843
Size: 228 615
BamHI G'GATC_C
Cuts at: 0 660 702 843
Size: 660 42 141
Fragments arranged by size:
660 141 42
BanI G'GyrC_C
Cuts at: 0 37 145 424 843
Size: 37 108 279 419
Fragments arranged by size:
419 279 108 37
BanII G_rGCy'C
Cuts at: 0 256 843
Size: 256 587
BbeI G_GCGC'C
Cuts at: 0 149 843
Size: 149 694
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6 40 843
Size: 6 34 803
Fragments arranged by size:
803 34 6
BglI GCCn_nnn'nGGC
Cuts at: 0 143 223 604 843
Size: 143 80 381 239
Fragments arranged by size:
381 239 143 80
Bme1580I G_kGCm'C
Cuts at: 0 40 843
Size: 40 803
BmgBI CAC'GTC
Cuts at: 0 214 843
Size: 214 629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 715 843
Size: 214 501 128
Fragments arranged by size:
501 214 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 843
Size: 331 512
BsaHI Gr'CG_yC
Cuts at: 0 146 386 843
Size: 146 240 457
Fragments arranged by size:
457 240 146
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 712 843
Size: 712 131
BseYI C'CCAG_C
Cuts at: 0 63 323 417 843
Size: 63 260 94 426
Fragments arranged by size:
426 260 94 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 145 654 843
Size: 28 117 509 189
Fragments arranged by size:
509 189 117 28
BsiHKAI G_wGCw'C
Cuts at: 0 200 843
Size: 200 643
BsmBI CGTCTCn'nnnn_
Cuts at: 0 478 843
Size: 478 365
Bsp1286I G_dGCh'C
Cuts at: 0 40 200 256 843
Size: 40 160 56 587
Fragments arranged by size:
587 160 56 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 843
Size: 173 670
BsrDI GCAATG_nn'
Cuts at: 0 269 843
Size: 269 574
BsrFI r'CCGG_y
Cuts at: 0 3 200 217 436 843
Size: 3 197 17 219 407
Fragments arranged by size:
407 219 197 17 3
BsrGI T'GTAC_A
Cuts at: 0 590 843
Size: 590 253
BssSI C'ACGA_G
Cuts at: 0 409 549 843
Size: 409 140 294
Fragments arranged by size:
409 294 140
BstYI r'GATC_y
Cuts at: 0 660 702 843
Size: 660 42 141
Fragments arranged by size:
660 141 42
BtgI C'CryG_G
Cuts at: 0 244 843
Size: 244 599
BtsI GCAGTG_nn'
Cuts at: 0 65 843
Size: 65 778
DraIII CAC_nnn'GTG
Cuts at: 0 414 843
Size: 414 429
EaeI y'GGCC_r
Cuts at: 0 219 843
Size: 219 624
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 250 715 843
Size: 214 36 465 128
Fragments arranged by size:
465 214 128 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 502 697 843
Size: 502 195 146
Fragments arranged by size:
502 195 146
EcoO109I rG'GnC_Cy
Cuts at: 0 225 288 606 843
Size: 225 63 318 237
Fragments arranged by size:
318 237 225 63
FspI TGC'GCA
Cuts at: 0 304 843
Size: 304 539
HaeII r_GCGC'y
Cuts at: 0 149 843
Size: 149 694
KasI G'GCGC_C
Cuts at: 0 145 843
Size: 145 698
MslI CAynn'nnrTG
Cuts at: 0 361 634 679 843
Size: 361 273 45 164
Fragments arranged by size:
361 273 164 45
MspA1I CmG'CkG
Cuts at: 0 12 246 843
Size: 12 234 597
Fragments arranged by size:
597 234 12
NaeI GCC'GGC
Cuts at: 0 219 438 843
Size: 219 219 405
Fragments arranged by size:
405 219 219
NarI GG'CG_CC
Cuts at: 0 146 843
Size: 146 697
NgoMIV G'CCGG_C
Cuts at: 0 217 436 843
Size: 217 219 407
Fragments arranged by size:
407 219 217
NspI r_CATG'y
Cuts at: 0 210 396 843
Size: 210 186 447
Fragments arranged by size:
447 210 186
PasI CC'CwG_GG
Cuts at: 0 742 843
Size: 742 101
PfoI T'CCnGG_A
Cuts at: 0 191 692 843
Size: 191 501 151
Fragments arranged by size:
501 191 151
PpuMI rG'GwC_Cy
Cuts at: 0 288 843
Size: 288 555
PstI C_TGCA'G
Cuts at: 0 503 843
Size: 503 340
PvuII CAG'CTG
Cuts at: 0 12 843
Size: 12 831
SacII CC_GC'GG
Cuts at: 0 247 843
Size: 247 596
SbfI CC_TGCA'GG
Cuts at: 0 503 843
Size: 503 340
SexAI A'CCwGG_T
Cuts at: 0 770 843
Size: 770 73
SfcI C'TryA_G
Cuts at: 0 499 843
Size: 499 344
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 604 843
Size: 604 239
SfoI GGC'GCC
Cuts at: 0 147 843
Size: 147 696
SgrAI Cr'CCGG_yG
Cuts at: 0 200 843
Size: 200 643
SmlI C'TyrA_G
Cuts at: 0 346 843
Size: 346 497
SphI G_CATG'C
Cuts at: 0 210 843
Size: 210 633
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 843
Size: 55 788
TatI w'GTAC_w
Cuts at: 0 590 843
Size: 590 253
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 112 144 843
Size: 112 32 699
Fragments arranged by size:
699 112 32
Tth111I GACn'n_nGTC
Cuts at: 0 772 843
Size: 772 71
XbaI T'CTAG_A
Cuts at: 0 832 843
Size: 832 11
Enzymes that do cut:
AarI AcuI AlfI AlwNI BamHI BanI BanII BbeI
BcgI BglI Bme1580I BmgBI BpmI BpuEI BsaHI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI
BsrGI BssSI BstYI BtgI BtsI DraIII EaeI Eco57MI
EcoNI EcoO109I FspI HaeII KasI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PfoI PpuMI PstI PvuII
SacII SbfI SexAI SfcI SfiI SfoI SgrAI SmlI
SphI TaqII TatI TstI Tth111I XbaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BspEI
BspHI BsrBI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI
EciI EcoICRI EcoRI EcoRV FalI FseI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI ScaI
SgfI SmaI SnaBI SpeI SrfI SspI StuI StyI
SwaI XcmI XhoI XmaI XmnI ZraI