Restriction map for
pFN21AE5625
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AvaII AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BsaAI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeII MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 1233 GTCTAC GT^[AC][GT]AC
AccII 810 CGCG CG^CG
AcsI 926 AAATTT [GA]^AATT[CT]
AfaI 846 GTAC GT^AC
AocI 1309 CCTGAGG CC^T[ACGT]AGG
ApaI 1383 GGGCCC GGGCC^C
ApoI 926 AAATTT [GA]^AATT[CT]
Asp700I 1165 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 190 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 296 TGGCCA TGG^CCA
BglI 985 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 177 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 177 GCTCAGC GC^T[ACGT]AGC
BsaBI 465 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 964 GAGACC GGTCTC(1/5)
BsaWI 1079 ACCGGA [AT]^CCGG[AT]
BsgI 814 GTGCAG GTGCAG(16/14)
BsmAI 964 GAGAC GTCTC(1/5)
BspHI 651 TCATGA T^CATGA
BspMI 457 ACCTGC ACCTGC(4/8)
BsrBI 104 GAGCGG CCGCTC(-3/-3)
BstUI 810 CGCG CG^CG
Bsu36I 1309 CCTGAGG CC^T[ACGT]AGG
CelII 177 GCTCAGC GC^T[ACGT]AGC
DsaI 698 CCACGG C^C[GA][CT]GG
EaeI 296 TGGCCA [CT]^GGCC[GA]
Eco81I 1309 CCTGAGG CC^T[ACGT]AGG
FauI 558 GCGGG CCCGC(4/6)
HgaI 274 GACGC GACGC(5/10)
HincII 334 GTCAAC GT[CT]^[GA]AC
HindII 334 GTCAAC GT[CT]^[GA]AC
MamI 465 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 296 TGGCCA TGG^CCA
MscI 296 TGGCCA TGG^CCA
MslI 1056 CATTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 810 CGCG CG^CG
NheI 1401 GCTAGC G^CTAGC
PflFI 190 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1274 CTGCAG CTGCA^G
RcaI 651 TCATGA T^CATGA
RsaI 846 GTAC GT^AC
SacI 1391 GAGCTC GAGCT^C
ScaI 845 AGTACT AGT^ACT
SfcI 1274 CTGCAG C^T[GA][CT]AG
SmaI 920 CCCGGG CCC^GGG
SspI 356 AATATT AAT^ATT
StuI 935 AGGCCT AGG^CCT
Tsp509I 927 AATT ^AATT
Tth111I 190 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1182 TCTAGA T^CTAGA
XmaCI 920 CCCGGG C^CCGGG
XmaI 920 CCCGGG C^CCGGG
XmnI 1165 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 395 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
973 CAGGAGCTG
AspHI 175 GTGCTC G[AT]GC[AT]^C
1391 GAGCTC
AvaI 725 CCCGAG C^[CT]CG[GA]G
920 CCCGGG
BanI 949 GGTGCC G^G[CT][GA]CC
1130 GGTGCC
BbvCI 420 CCTCAGC CCTCAGC(-5/-2)
571 CCTCAGC
BcnI 920 CCCGG CC^[GC]GG
921 CCGGG
BpmI 778 CTGGAG CTGGAG(16/14)
877 CTGGAG
BsiHKAI 175 GTGCTC G[AT]GC[AT]^C
1391 GAGCTC
BsoBI 725 CCCGAG C^[CT]CG[GA]G
920 CCCGGG
BsrDI 528 CATTGC GCAATG(2/0)
606 CATTGC
BstXI 1253 CCAAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1371 CCAAGACCCTGG
EarI 71 CTCTTC CTCTTC(1/4)
708 CTCTTC
Ksp632I 71 CTCTTC CTCTTC(1/4)
708 CTCTTC
NciI 920 CCCGG CC^[GC]GG
921 CCGGG
PleI 69 GACTC GAGTC(4/5)
880 GAGTC
SmlI 139 CTCAAG C^T[CT][GA]AG
757 CTCAAG
TfiI 147 GATTC G^A[AT]TC
465 GATTC
Tsp45I 454 GTGAC ^GT[GC]AC
1207 GTCAC
TspRI 151 CAGTG CA[GC]TG[ACGT][ACGT]^
185 CAGTG
XcmI 365 CCAAGGGAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
968 CCACACAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwI BanII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PvuII Sau3AI Sau96I ScrFI
SfaNI StyI TaqI Tru9I TseI
TthHB8I XhoII