(Circular) (Six-base) MAPSORT of: pFN21AE5625.seq Check: 8451 from: 1 to: 6184 pFN21AE5625 6184 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3994 278 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3239 3527 1937 Size: 1302 288 4594 Fragments arranged by size: 4594 1302 288 Acc65I G'GTAC_C Cuts at: 3505 3505 Size: 6184 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2259 3003 3063 4247 5631 5899 1632 Size: 627 744 60 1184 1384 268 1917 Fragments arranged by size: 1917 1384 1184 744 627 268 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5987 AflIII A'CryG_T Cuts at: 1203 5083 6060 1203 Size: 3880 977 1327 Fragments arranged by size: 3880 1327 977 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 4913 4913 Size: 6184 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6184 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6184 AlwNI CAG_nnn'CTG Cuts at: 2405 2983 3927 5499 2405 Size: 578 944 1572 3090 Fragments arranged by size: 3090 1572 944 578 ApaI G_GGCC'C Cuts at: 3392 3392 Size: 6184 ApaLI G'TGCA_C Cuts at: 4241 5397 4241 Size: 1156 5028 ApoI r'AATT_y Cuts at: 1493 2931 3493 3719 3871 5980 1493 Size: 1438 562 226 152 2109 1697 Fragments arranged by size: 2109 1697 1438 562 226 152 AseI AT'TA_AT Cuts at: 160 1032 4738 160 Size: 872 3706 1606 Fragments arranged by size: 3706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 2730 2925 3509 1870 Size: 73 787 195 584 4545 Fragments arranged by size: 4545 787 584 195 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3215 3248 3215 Size: 33 6151 BamHI G'GATC_C Cuts at: 3514 3881 3514 Size: 367 5817 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2954 3135 3505 4960 618 Size: 325 217 627 1167 181 370 1455 1842 Fragments arranged by size: 1842 1455 1167 627 370 325 217 181 BanII G_rGCy'C Cuts at: 1391 2293 3341 3392 3400 3503 1391 Size: 902 1048 51 8 103 4072 Fragments arranged by size: 4072 1048 902 103 51 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2215 2251 2913 928 Size: 971 316 36 662 4199 Fragments arranged by size: 4199 971 662 316 36 BbvCI CC'TCA_GC Cuts at: 2426 2577 2426 Size: 151 6033 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4375 4409 1903 Size: 34 2438 34 3678 Fragments arranged by size: 3678 2438 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4076 5286 4076 Size: 1210 4974 BclI T'GATC_A Cuts at: 1548 1548 Size: 6184 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2996 4795 136 Size: 107 122 71 631 605 1324 1799 1525 Fragments arranged by size: 1799 1525 1324 631 605 122 107 71 BglII A'GATC_T Cuts at: 6179 6179 Size: 6184 BlpI GC'TnA_GC Cuts at: 2183 3601 2183 Size: 1418 4766 Bme1580I G_kGCm'C Cuts at: 1790 3392 4245 5401 1790 Size: 1602 853 1156 2573 Fragments arranged by size: 2573 1602 1156 853 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6184 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2911 3915 4873 Size: 416 53 83 399 24 1494 1004 958 Cuts at: 4873 442 Size: 1753 Fragments arranged by size: 1753 1494 1004 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3410 1056 Size: 2354 3830 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3384 3384 Size: 6184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2804 2903 4844 1239 Size: 1565 99 1941 2579 Fragments arranged by size: 2579 1941 1565 99 Bpu10I CC'TnA_GC Cuts at: 2426 2501 2577 3399 2426 Size: 75 76 822 5211 Fragments arranged by size: 5211 822 76 75 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2129 2747 4309 5174 5472 2129 Size: 618 1562 865 298 2841 Fragments arranged by size: 2841 1562 865 618 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2963 4847 882 Size: 617 1464 1884 2219 Fragments arranged by size: 2219 1884 1464 617 BsaAI yAC'GTr Cuts at: 493 1204 3975 493 Size: 711 2771 2702 Fragments arranged by size: 2771 2702 711 BsaBI GATnn'nnATC Cuts at: 2474 6178 2474 Size: 3704 2480 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3991 4373 5008 275 Size: 53 83 186 936 2458 382 635 1451 Fragments arranged by size: 2458 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3084 4617 5015 5289 5436 5847 3084 Size: 1533 398 274 147 411 3421 Fragments arranged by size: 3421 1533 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5959 Fragments arranged by size: 5959 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 2582 2801 2981 3193 3347 1186 Size: 958 438 219 180 212 154 4023 Fragments arranged by size: 4023 958 438 219 212 180 154 BseYI C'CCAG_C Cuts at: 2991 3097 3325 3480 5387 5883 2991 Size: 106 228 155 1907 496 3292 Fragments arranged by size: 3292 1907 496 228 155 106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2840 1163 Size: 573 1104 4507 Fragments arranged by size: 4507 1104 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3634 4395 5423 1540 Size: 275 186 1633 761 1028 2301 Fragments arranged by size: 2301 1633 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2184 3400 4245 4330 5401 2184 Size: 1216 845 85 1071 2967 Fragments arranged by size: 2967 1216 1071 845 85 BsmI GAATG_Cn' Cuts at: 3695 3788 3695 Size: 93 6091 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 2293 3341 3392 3400 3503 4245 Size: 399 394 109 1048 51 8 103 742 Cuts at: 4245 4330 5401 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1048 742 399 394 109 103 85 51 8 BspEI T'CCGG_A Cuts at: 5847 5847 Size: 6184 BspHI T'CATG_A Cuts at: 2656 4073 5803 2656 Size: 1417 1730 3037 Fragments arranged by size: 3037 1730 1417 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2471 3539 844 Size: 1627 1068 3489 Fragments arranged by size: 3489 1627 1068 BsrBI CCG'CTC Cuts at: 1394 1823 2111 4071 1394 Size: 429 288 1960 3507 Fragments arranged by size: 3507 1960 429 288 BsrDI GCAATG_nn' Cuts at: 65 1248 2532 2610 4679 4853 65 Size: 1183 1284 78 2069 174 1396 Fragments arranged by size: 2069 1396 1284 1183 174 78 BsrFI r'CCGG_y Cuts at: 1700 1810 4828 5015 1700 Size: 110 3018 187 2869 Fragments arranged by size: 3018 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6184 BssSI C'ACGA_G Cuts at: 4244 5256 4244 Size: 1012 5172 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6184 BstBI TT'CG_AA Cuts at: 4988 4988 Size: 6184 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6184 BstXI CCAn_nnnn'nTGG Cuts at: 3265 3383 3265 Size: 118 6066 BstYI r'GATC_y Cuts at: 1972 2421 2651 3421 3514 3881 4266 4283 5735 Size: 449 230 770 93 367 385 17 1452 Cuts at: 5735 5821 5833 6179 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 770 449 385 367 346 230 93 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3315 3315 Size: 6184 BtgI C'CryG_G Cuts at: 513 1065 2703 513 Size: 552 1638 3994 Fragments arranged by size: 3994 1638 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2469 2527 487 Size: 683 56 1243 58 4144 Fragments arranged by size: 4144 1243 683 58 56 BtsI GCAGTG_nn' Cuts at: 3700 4494 4514 3700 Size: 794 20 5370 Fragments arranged by size: 5370 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6149 DraI TTT'AAA Cuts at: 3488 3488 Size: 6184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5191 809 Size: 507 3875 1802 Fragments arranged by size: 3875 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2301 3631 4520 Size: 54 1425 50 275 18 471 1330 889 Cuts at: 4520 6149 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1330 889 471 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3631 1537 Size: 275 1819 4090 Fragments arranged by size: 4090 1819 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2082 2719 4114 1353 Size: 729 637 1395 3423 Fragments arranged by size: 3423 1395 729 637 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3284 4771 5145 5291 387 Size: 1064 138 1695 1487 374 146 1280 Fragments arranged by size: 1695 1487 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3398 3398 Size: 6184 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2259 2804 2903 3003 3063 4247 4844 Size: 393 627 545 99 100 60 1184 597 Cuts at: 4844 5631 5899 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1184 787 627 597 545 393 268 100 99 60 EcoNI CCTnn'n_nnAGG Cuts at: 2765 2786 3314 2765 Size: 21 528 5635 Fragments arranged by size: 5635 528 21 EcoO109I rG'GnC_Cy Cuts at: 3307 3388 3389 3307 Size: 81 1 6102 Fragments arranged by size: 6102 81 1 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2872 3454 2872 Size: 582 5602 FspI TGC'GCA Cuts at: 4690 4690 Size: 6184 HaeII r_GCGC'y Cuts at: 1678 5331 1678 Size: 3653 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2609 2641 4825 4857 4899 Size: 32 135 32 1037 32 2184 32 42 Cuts at: 4899 4931 1373 Size: 32 2626 Fragments arranged by size: 2626 2184 1037 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2341 3528 3774 669 Size: 988 281 403 1187 246 3079 Fragments arranged by size: 3079 1187 988 403 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6184 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KpnI G_GTAC'C Cuts at: 3509 3509 Size: 6184 MfeI C'AATT_G Cuts at: 3783 3783 Size: 6184 MluI A'CGCG_T Cuts at: 6060 6060 Size: 6184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2692 5298 5482 1305 Size: 1387 2606 184 2007 Fragments arranged by size: 2606 2007 1387 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2303 10 Size: 54 1425 343 471 3891 Fragments arranged by size: 3891 1425 471 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3065 4142 4501 4660 518 Size: 648 1899 1077 359 159 2042 Fragments arranged by size: 2042 1899 1077 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2592 2881 3041 3600 4277 5425 5670 1606 Size: 986 289 160 559 677 1148 245 2120 Fragments arranged by size: 2120 1148 986 677 559 289 245 160 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5632 NdeI CA'TA_TG Cuts at: 387 387 Size: 6184 NheI G'CTAG_C Cuts at: 1052 3406 1052 Size: 2354 3830 NotI GC'GGCC_GC Cuts at: 3631 3631 Size: 6184 NspI r_CATG'y Cuts at: 1132 1229 3542 5087 1132 Size: 97 2313 1545 2229 Fragments arranged by size: 2313 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2893 3383 1348 Size: 1545 490 4149 Fragments arranged by size: 4149 1545 490 PciI A'CATG_T Cuts at: 5083 5083 Size: 6184 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6184 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4253 4285 5766 5798 4253 Size: 32 1481 32 4639 Fragments arranged by size: 4639 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6184 PsiI TTA'TAA Cuts at: 3754 3754 Size: 6184 PspOMI G'GGCC_C Cuts at: 3388 3388 Size: 6184 PstI C_TGCA'G Cuts at: 830 3283 3536 830 Size: 2453 253 3478 Fragments arranged by size: 3478 2453 253 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 PvuII CAG'CTG Cuts at: 2592 2881 3041 2592 Size: 289 160 5735 Fragments arranged by size: 5735 289 160 SacI G_AGCT'C Cuts at: 3400 3400 Size: 6184 SalI G'TCGA_C Cuts at: 1936 3526 1936 Size: 1590 4594 SbfI CC_TGCA'GG Cuts at: 3536 3536 Size: 6184 ScaI AGT'ACT Cuts at: 2852 4432 6144 2852 Size: 1580 1712 2892 Fragments arranged by size: 2892 1712 1580 SfcI C'TryA_G Cuts at: 826 1045 3279 3532 4667 5348 5539 826 Size: 219 2234 253 1135 681 191 1471 Fragments arranged by size: 2234 1471 1135 681 253 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6184 SmaI CCC'GGG Cuts at: 1872 2927 3511 1872 Size: 1055 584 4545 Fragments arranged by size: 4545 1055 584 SmlI C'TyrA_G Cuts at: 820 1017 1943 2144 2762 4288 5189 5451 820 Size: 197 926 201 618 1526 901 262 1553 Fragments arranged by size: 1553 1526 926 901 618 262 201 197 SnaBI TAC'GTA Cuts at: 493 3975 493 Size: 3482 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 3542 1132 Size: 2410 3774 SspI AAT'ATT Cuts at: 5 52 2363 4108 5 Size: 47 2311 1745 2081 Fragments arranged by size: 2311 2081 1745 47 StuI AGG'CCT Cuts at: 1517 2942 1517 Size: 1425 4759 StyI C'CwwG_G Cuts at: 513 1065 2370 2950 3447 3623 513 Size: 552 1305 580 497 176 3074 Fragments arranged by size: 3074 1305 580 552 497 176 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3317 4211 4228 4381 4566 5011 325 Size: 1255 1737 894 17 153 185 445 1498 Fragments arranged by size: 1737 1498 1255 894 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2850 4430 6142 Size: 274 80 33 51 1443 873 1580 1712 Cuts at: 6142 96 Size: 138 Fragments arranged by size: 1712 1580 1443 873 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6144 6176 6144 Size: 32 6152 Tth111I GACn'n_nGTC Cuts at: 1316 2198 1316 Size: 882 5302 XbaI T'CTAG_A Cuts at: 3187 3520 3187 Size: 333 5851 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2377 2980 2377 Size: 603 5581 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6184 XmaI C'CCGG_G Cuts at: 1870 2925 3509 1870 Size: 1055 584 4545 Fragments arranged by size: 4545 1055 584 XmnI GAAnn'nnTTC Cuts at: 3174 4313 3174 Size: 1139 5045 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3992 276 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI