(Linear) (Six-base) MAPSORT of: KIEE5625.seq Check: 4382 from: 1 to: 1479 KIEE5625.orf With 182 enzymes: SgfI * December 5, 2014 13:03 .. AccI GT'mk_AC Cuts at: 0 1234 1479 Size: 1234 245 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 254 998 1058 1479 Size: 254 744 60 421 Fragments arranged by size: 744 421 254 60 AlwNI CAG_nnn'CTG Cuts at: 0 400 978 1479 Size: 400 578 501 Fragments arranged by size: 578 501 400 ApaI G_GGCC'C Cuts at: 0 1387 1479 Size: 1387 92 ApoI r'AATT_y Cuts at: 0 926 1479 Size: 926 553 AvaI C'yCGr_G Cuts at: 0 725 920 1479 Size: 725 195 559 Fragments arranged by size: 725 559 195 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1210 1243 1479 Size: 1210 33 236 Fragments arranged by size: 1210 236 33 BanI G'GyrC_C Cuts at: 0 949 1130 1479 Size: 949 181 349 Fragments arranged by size: 949 349 181 BanII G_rGCy'C Cuts at: 0 288 1336 1387 1395 1479 Size: 288 1048 51 8 84 Fragments arranged by size: 1048 288 84 51 8 BbsI GAAGACnn'nnnn_ Cuts at: 0 210 246 908 1479 Size: 210 36 662 571 Fragments arranged by size: 662 571 210 36 BbvCI CC'TCA_GC Cuts at: 0 421 572 1479 Size: 421 151 907 Fragments arranged by size: 907 421 151 BglI GCCn_nnn'nGGC Cuts at: 0 991 1479 Size: 991 488 BlpI GC'TnA_GC Cuts at: 0 178 1479 Size: 178 1301 Bme1580I G_kGCm'C Cuts at: 0 1387 1479 Size: 1387 92 BmrI ACTGGGnnnn_n' Cuts at: 0 906 1479 Size: 906 573 BmtI G_CTAG'C Cuts at: 0 1405 1479 Size: 1405 74 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1379 1479 Size: 1379 100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 799 898 1479 Size: 799 99 581 Fragments arranged by size: 799 581 99 Bpu10I CC'TnA_GC Cuts at: 0 421 496 572 1394 1479 Size: 421 75 76 822 85 Fragments arranged by size: 822 421 85 76 75 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 742 1479 Size: 124 618 737 Fragments arranged by size: 737 618 124 BsaI GGTCTCn'nnnn_ Cuts at: 0 958 1479 Size: 958 521 BsaBI GATnn'nnATC Cuts at: 0 469 1479 Size: 469 1010 BsaWI w'CCGG_w Cuts at: 0 1079 1479 Size: 1079 400 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 139 577 796 976 1188 1342 1479 Size: 139 438 219 180 212 154 137 Fragments arranged by size: 438 219 212 180 154 139 137 BseYI C'CCAG_C Cuts at: 0 986 1092 1320 1479 Size: 986 106 228 159 Fragments arranged by size: 986 228 159 106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 835 1479 Size: 835 644 BsiHKAI G_wGCw'C Cuts at: 0 179 1395 1479 Size: 179 1216 84 Fragments arranged by size: 1216 179 84 Bsp1286I G_dGCh'C Cuts at: 0 179 288 1336 1387 1395 1479 Size: 179 109 1048 51 8 84 Fragments arranged by size: 1048 179 109 84 51 8 BspHI T'CATG_A Cuts at: 0 651 1479 Size: 651 828 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 466 1479 Size: 466 1013 BsrBI CCG'CTC Cuts at: 0 106 1479 Size: 106 1373 BsrDI GCAATG_nn' Cuts at: 0 527 605 1479 Size: 527 78 874 Fragments arranged by size: 874 527 78 BstXI CCAn_nnnn'nTGG Cuts at: 0 1260 1378 1479 Size: 1260 118 101 Fragments arranged by size: 1260 118 101 BstYI r'GATC_y Cuts at: 0 416 646 1416 1479 Size: 416 230 770 63 Fragments arranged by size: 770 416 230 63 Bsu36I CC'TnA_GG Cuts at: 0 1310 1479 Size: 1310 169 BtgI C'CryG_G Cuts at: 0 698 1479 Size: 698 781 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 464 522 1479 Size: 464 58 957 Fragments arranged by size: 957 464 58 EaeI y'GGCC_r Cuts at: 0 296 1479 Size: 296 1183 EarI CTCTTCn'nnn_ Cuts at: 0 77 714 1479 Size: 77 637 765 Fragments arranged by size: 765 637 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1279 1479 Size: 1279 200 EcoICRI GAG'CTC Cuts at: 0 1393 1479 Size: 1393 86 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 254 799 898 998 1058 1479 Size: 254 545 99 100 60 421 Fragments arranged by size: 545 421 254 100 99 60 EcoNI CCTnn'n_nnAGG Cuts at: 0 760 781 1309 1479 Size: 760 21 528 170 Fragments arranged by size: 760 528 170 21 EcoO109I rG'GnC_Cy Cuts at: 0 1302 1383 1384 1479 Size: 1302 81 1 95 Fragments arranged by size: 1302 95 81 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 867 1449 1479 Size: 867 582 30 Fragments arranged by size: 867 582 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 604 636 1479 Size: 604 32 843 Fragments arranged by size: 843 604 32 HincII GTy'rAC Cuts at: 0 336 1479 Size: 336 1143 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 687 1479 Size: 687 792 MscI TGG'CCA Cuts at: 0 298 1479 Size: 298 1181 MslI CAynn'nnrTG Cuts at: 0 1060 1479 Size: 1060 419 MspA1I CmG'CkG Cuts at: 0 587 876 1036 1479 Size: 587 289 160 443 Fragments arranged by size: 587 443 289 160 NheI G'CTAG_C Cuts at: 0 1401 1479 Size: 1401 78 PasI CC'CwG_GG Cuts at: 0 888 1378 1479 Size: 888 490 101 Fragments arranged by size: 888 490 101 PspOMI G'GGCC_C Cuts at: 0 1383 1479 Size: 1383 96 PstI C_TGCA'G Cuts at: 0 1278 1479 Size: 1278 201 PvuII CAG'CTG Cuts at: 0 587 876 1036 1479 Size: 587 289 160 443 Fragments arranged by size: 587 443 289 160 SacI G_AGCT'C Cuts at: 0 1395 1479 Size: 1395 84 ScaI AGT'ACT Cuts at: 0 847 1479 Size: 847 632 SfcI C'TryA_G Cuts at: 0 1274 1479 Size: 1274 205 SmaI CCC'GGG Cuts at: 0 922 1479 Size: 922 557 SmlI C'TyrA_G Cuts at: 0 139 757 1479 Size: 139 618 722 Fragments arranged by size: 722 618 139 SspI AAT'ATT Cuts at: 0 358 1479 Size: 358 1121 StuI AGG'CCT Cuts at: 0 937 1479 Size: 937 542 StyI C'CwwG_G Cuts at: 0 365 945 1442 1479 Size: 365 580 497 37 Fragments arranged by size: 580 497 365 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1312 1479 Size: 1312 167 TatI w'GTAC_w Cuts at: 0 845 1479 Size: 845 634 Tth111I GACn'n_nGTC Cuts at: 0 193 1479 Size: 193 1286 XbaI T'CTAG_A Cuts at: 0 1182 1479 Size: 1182 297 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 372 975 1479 Size: 372 603 504 Fragments arranged by size: 603 504 372 XmaI C'CCGG_G Cuts at: 0 920 1479 Size: 920 559 XmnI GAAnn'nnTTC Cuts at: 0 1169 1479 Size: 1169 310 Enzymes that do cut: AccI AcuI AlwNI ApaI ApoI AvaI BaeI BanI BanII BbsI BbvCI BglI BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BstXI BstYI Bsu36I BtgI BtgZI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII MmeI MscI MslI MspA1I NheI PasI PspOMI PstI PvuII SacI ScaI SfcI SmaI SmlI SspI StuI StyI TaqII TatI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BamHI BbeI BcgI BciVI BclI BfrBI BglII BmgBI BsaAI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI TstI XhoI ZraI