################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AvrII BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII KpnI KspI MaeI MamI MfeI MluI MroI MslI MunI MvnI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XcmI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 769 GTAGAC GT^[AC][GT]AC AcsI 1069 AAATTC [GA]^AATT[CT] AflIII 1091 ACGTGT A^C[GA][CT]GT ApoI 1069 AAATTC [GA]^AATT[CT] Asp700I 1147 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1307 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 326 TGCGCA TGC^GCA BamHI 1024 GGATCC G^GATCC BglI 374 GCCTCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1263 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1263 GCTGAGC GC^T[ACGT]AGC BsaAI 599 TACGTA [CT]AC^GT[GA] BseRI 1117 GAGGAG GAGGAG(10/8) BsrBI 250 CCGCTC CCGCTC(-3/-3) BsrFI 1063 GCCGGC [GA]^CCGG[CT] BstAPI 1268 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1369 CCAGCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1024 GGATCC [GA]^GATC[CT] CelII 1263 GCTGAGC GC^T[ACGT]AGC Cfr10I 1063 GCCGGC [GA]^CCGG[CT] DsaI 944 CCATGG C^C[GA][CT]GG EarI 1147 GAAGAG CTCTTC(1/4) FspI 326 TGCGCA TGC^GCA HincII 319 GTTAAC GT[CT]^[GA]AC HindII 319 GTTAAC GT[CT]^[GA]AC HpaI 319 GTTAAC GTT^AAC Ksp632I 1147 GAAGAG CTCTTC(1/4) MflI 1024 GGATCC [GA]^GATC[CT] MseI 320 TTAA T^TAA NaeI 1063 GCCGGC GCC^GGC NcoI 944 CCATGG C^CATGG NgoMIV 1063 GCCGGC G^CCGGC PaeR7I 16 CTCGAG C^TCGAG PflFI 1307 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 21 GGGTCCC [GA]G^G[AT]CC[CT] PstI 898 CTGCAG CTGCA^G PvuII 1261 CAGCTG CAG^CTG SacI 669 GAGCTC GAGCT^C SapI 1147 GAAGAGC GCTCTTC(1/4) SmaI 854 CCCGGG CCC^GGG SmlI 16 CTCGAG C^T[CT][GA]AG SnaBI 599 TACGTA TAC^GTA TfiI 1341 GATTC G^A[AT]TC Tru9I 320 TTAA T^TAA Tth111I 1307 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 16 CTCGAG C^TCGAG XhoII 1024 GGATCC [GA]^GATC[CT] XmaCI 854 CCCGGG C^CCGGG XmaI 854 CCCGGG C^CCGGG XmnI 1147 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AfaI 602 GTAC GT^AC 1207 GTAC AlwI 1025 GATCC GGATC(4/5) 1024 GGATC AocI 285 CCTAAGG CC^T[ACGT]AGG 1075 CCTCAGG ApaI 230 GGGCCC GGGCC^C 806 GGGCCC AspHI 669 GAGCTC G[AT]GC[AT]^C 872 GTGCTC AvaI 16 CTCGAG C^[CT]CG[GA]G 854 CCCGGG BalI 567 TGGCCA TGG^CCA 1427 TGGCCA BbeI 139 GGCGCC GGCGC^C 382 GGCGCC BbsI 1087 GAAGAC GAAGAC(2/6) 1186 GTCTTC BpuAI 1087 GAAGAC GAAGAC(2/6) 1186 GTCTTC BsiHKAI 669 GAGCTC G[AT]GC[AT]^C 872 GTGCTC BsoBI 16 CTCGAG C^[CT]CG[GA]G 854 CCCGGG BssSI 253 CTCGTG CACGAG(-5/-1) 376 CTCGTG Bsu36I 285 CCTAAGG CC^T[ACGT]AGG 1075 CCTCAGG Eco81I 285 CCTAAGG CC^T[ACGT]AGG 1075 CCTCAGG EcoNI 285 CCTAAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1110 CCTGATAGAGG EcoT14I 944 CCATGG C^C[AT][AT]GG 1235 CCTTGG HaeII 139 GGCGCC [GA]GCGC^[CT] 382 GGCGCC KasI 139 GGCGCC G^GCGCC 382 GGCGCC MluNI 567 TGGCCA TGG^CCA 1427 TGGCCA MscI 567 TGGCCA TGG^CCA 1427 TGGCCA MspA1I 708 CAGCGG C[AC]G^C[GT]G 1261 CAGCTG NarI 139 GGCGCC GG^CGCC 382 GGCGCC NspI 473 GCATGT [GA]CATG^[CT] 611 ACATGC PflMI 570 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1346 CCATACACTGG RsaI 602 GTAC GT^AC 1207 GTAC SfcI 310 CTACAG C^T[GA][CT]AG 898 CTGCAG SfoI 139 GGCGCC GGC^GCC 382 GGCGCC StyI 944 CCATGG C^C[AT][AT]GG 1235 CCTTGG Tsp45I 505 GTGAC ^GT[GC]AC 819 GTCAC Van91I 570 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1346 CCATACACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcyI AluI AlwNI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp509I TspRI TthHB8I