(Circular) (Six-base) MAPSORT of: pFN21AE1940.seq Check: 3029 from: 1 to: 6163 pFN21AE1940 6163 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2660 2660 Size: 6163 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3973 278 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2775 3506 1937 Size: 838 731 4594 Fragments arranged by size: 4594 838 731 Acc65I G'GTAC_C Cuts at: 3484 3484 Size: 6163 AclI AA'CG_TT Cuts at: 1563 4290 4663 1563 Size: 2727 373 3063 Fragments arranged by size: 3063 2727 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2933 3143 3178 3309 4226 5610 5878 1632 Size: 1301 210 35 131 917 1384 268 1917 Fragments arranged by size: 1917 1384 1301 917 268 210 131 35 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5966 AflIII A'CryG_T Cuts at: 1203 3096 5062 6039 1203 Size: 1893 1966 977 1327 Fragments arranged by size: 1966 1893 1327 977 AgeI A'CCGG_T Cuts at: 4994 4994 Size: 6163 AhdI GACnn_n'nnGTC Cuts at: 4892 4892 Size: 6163 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6163 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6163 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3236 3268 3236 Size: 32 6131 AlwNI CAG_nnn'CTG Cuts at: 2218 2798 2911 3088 3279 3319 3906 5478 2218 Size: 580 113 177 191 40 587 1572 2903 Fragments arranged by size: 2903 1572 587 580 191 177 113 40 ApaI G_GGCC'C Cuts at: 2239 2815 2239 Size: 576 5587 ApaLI G'TGCA_C Cuts at: 4220 5376 4220 Size: 1156 5007 ApoI r'AATT_y Cuts at: 1493 3074 3472 3698 3850 5959 1493 Size: 1581 398 226 152 2109 1697 Fragments arranged by size: 2109 1697 1581 398 226 152 AseI AT'TA_AT Cuts at: 160 1032 4717 160 Size: 872 3685 1606 Fragments arranged by size: 3685 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 AvaI C'yCGr_G Cuts at: 1870 1943 2021 2859 3488 1870 Size: 73 78 838 629 4545 Fragments arranged by size: 4545 838 629 78 73 BamHI G'GATC_C Cuts at: 3029 3493 3860 3029 Size: 464 367 5332 Fragments arranged by size: 5332 464 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2144 2171 2387 2830 3484 Size: 325 217 627 357 27 216 443 654 Cuts at: 3484 4939 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 654 627 443 357 325 217 216 27 BanII G_rGCy'C Cuts at: 1391 2131 2158 2239 2678 2815 3482 1391 Size: 740 27 81 439 137 667 4072 Fragments arranged by size: 4072 740 667 439 137 81 27 BbeI G_GCGC'C Cuts at: 2148 2391 2148 Size: 243 5920 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3099 3184 928 Size: 971 1200 85 3907 Fragments arranged by size: 3907 1200 971 85 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4354 4388 1903 Size: 34 50 34 2333 34 3678 Fragments arranged by size: 3678 2333 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4055 5265 4055 Size: 1210 4953 BclI T'GATC_A Cuts at: 1548 1548 Size: 6163 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2385 4774 136 Size: 107 122 71 631 605 713 2389 1525 Fragments arranged by size: 2389 1525 713 631 605 122 107 71 BglII A'GATC_T Cuts at: 6158 6158 Size: 6163 BlpI GC'TnA_GC Cuts at: 3269 3580 3269 Size: 311 5852 Bme1580I G_kGCm'C Cuts at: 1790 2174 2239 2815 4224 5380 1790 Size: 384 65 576 1409 1156 2573 Fragments arranged by size: 2573 1409 1156 576 384 65 BmgBI CAC'GTC Cuts at: 1318 3097 3299 1318 Size: 1779 202 4182 Fragments arranged by size: 4182 1779 202 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2386 3410 3437 Size: 416 53 83 399 24 969 1024 27 Cuts at: 3437 3894 4852 442 Size: 457 958 1753 Fragments arranged by size: 1753 1024 969 958 457 416 399 83 53 27 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6163 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2396 2396 Size: 6163 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2474 2522 3163 4823 1239 Size: 830 405 48 641 1660 2579 Fragments arranged by size: 2579 1660 830 641 405 48 Bpu10I CC'TnA_GC Cuts at: 2682 2682 Size: 6163 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4288 5153 5451 4288 Size: 865 298 5000 Fragments arranged by size: 5000 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4826 882 Size: 617 3327 2219 Fragments arranged by size: 3327 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2606 3954 493 Size: 711 1402 1348 2702 Fragments arranged by size: 2702 1402 1348 711 BsaBI GATnn'nnATC Cuts at: 6157 6157 Size: 6163 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2145 2388 2688 2928 Size: 53 83 186 936 612 243 300 240 Cuts at: 2928 3970 4352 4987 275 Size: 1042 382 635 1451 Fragments arranged by size: 1451 1042 936 635 612 382 300 243 240 186 83 53 BsaWI w'CCGG_w Cuts at: 4596 4994 5268 5415 5826 4596 Size: 398 274 147 411 4933 Fragments arranged by size: 4933 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2164 2194 3236 3266 1178 Size: 30 165 30 761 30 1042 30 4075 Fragments arranged by size: 4075 1042 761 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3137 1186 Size: 1951 4212 BseYI C'CCAG_C Cuts at: 2140 2204 2620 5366 5862 2140 Size: 64 416 2746 496 2441 Fragments arranged by size: 2746 2441 496 416 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5590 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3613 4374 5402 1540 Size: 275 186 1612 761 1028 2301 Fragments arranged by size: 2301 1612 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2678 2881 4224 4309 5380 2678 Size: 203 1343 85 1071 3461 Fragments arranged by size: 3461 1343 1071 203 85 BsmI GAATG_Cn' Cuts at: 3674 3767 3674 Size: 93 6070 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2131 2158 2174 2239 2678 2815 2881 Size: 399 341 27 16 65 439 137 66 Cuts at: 2881 3482 4224 4309 5380 1391 Size: 601 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 601 439 399 341 137 85 66 65 27 16 BspEI T'CCGG_A Cuts at: 5826 5826 Size: 6163 BspHI T'CATG_A Cuts at: 4052 5782 4052 Size: 1730 4433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2286 2287 2337 2660 3189 3518 844 Size: 1442 1 50 323 529 329 3489 Fragments arranged by size: 3489 1442 529 329 323 50 1 BsrBI CCG'CTC Cuts at: 1394 1823 2257 4050 1394 Size: 429 434 1793 3507 Fragments arranged by size: 3507 1793 434 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4658 4832 65 Size: 1183 3410 174 1396 Fragments arranged by size: 3410 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3068 4807 4994 1700 Size: 110 1258 1739 187 2869 Fragments arranged by size: 2869 1739 1258 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6163 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6163 BssSI C'ACGA_G Cuts at: 2258 2381 4223 5235 2258 Size: 123 1842 1012 3186 Fragments arranged by size: 3186 1842 1012 123 BstAPI GCAn_nnn'nTGC Cuts at: 794 3279 794 Size: 2485 3678 BstBI TT'CG_AA Cuts at: 4967 4967 Size: 6163 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6163 BstXI CCAn_nnnn'nTGG Cuts at: 3381 3381 Size: 6163 BstYI r'GATC_y Cuts at: 1972 3029 3493 3860 4245 4262 5714 5800 5812 Size: 1057 464 367 385 17 1452 86 12 Cuts at: 5812 6158 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1057 464 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2291 3081 2291 Size: 790 5373 BtgI C'CryG_G Cuts at: 513 1065 2949 513 Size: 552 1884 3727 Fragments arranged by size: 3727 1884 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5424 Fragments arranged by size: 5424 683 56 BtsI GCAGTG_nn' Cuts at: 3679 4473 4493 3679 Size: 794 20 5349 Fragments arranged by size: 5349 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6163 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6128 DraI TTT'AAA Cuts at: 3467 3467 Size: 6163 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5170 809 Size: 507 3854 1802 Fragments arranged by size: 3854 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2266 2347 2572 Size: 54 1425 50 275 18 436 81 225 Cuts at: 2572 3432 3610 4499 6128 8 Size: 860 178 889 1629 43 Fragments arranged by size: 1629 1425 889 860 436 275 225 178 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3610 1537 Size: 275 1798 4090 Fragments arranged by size: 4090 1798 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3147 4093 1353 Size: 1794 946 3423 Fragments arranged by size: 3423 1794 946 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4750 5124 5270 387 Size: 1064 138 3161 374 146 1280 Fragments arranged by size: 3161 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2676 2676 Size: 6163 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2474 2522 2933 3143 3163 3178 Size: 393 437 405 48 411 210 20 15 Cuts at: 3178 3309 4226 4823 5610 5878 1239 Size: 131 917 597 787 268 1524 Fragments arranged by size: 1524 917 787 597 437 411 405 393 268 210 131 48 20 15 EcoNI CCTnn'n_nnAGG Cuts at: 2294 3119 2294 Size: 825 5338 EcoO109I rG'GnC_Cy Cuts at: 2027 2117 2215 2236 2812 2027 Size: 90 98 21 576 5378 Fragments arranged by size: 5378 576 98 90 21 EcoRI G'AATT_C Cuts at: 3472 3850 3472 Size: 378 5785 FspI TGC'GCA Cuts at: 2333 4669 2333 Size: 2336 3827 HaeII r_GCGC'y Cuts at: 1678 2148 2391 5310 1678 Size: 470 243 2919 2531 Fragments arranged by size: 2919 2531 470 243 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3301 3333 4804 4836 4878 Size: 32 135 32 1729 32 1471 32 42 Cuts at: 4878 4910 1373 Size: 32 2626 Fragments arranged by size: 2626 1729 1471 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2326 3507 3753 669 Size: 988 281 388 1181 246 3079 Fragments arranged by size: 3079 1181 988 388 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6163 HpaI GTT'AAC Cuts at: 2326 3753 2326 Size: 1427 4736 KasI G'GCGC_C Cuts at: 2144 2387 2144 Size: 243 5920 KpnI G_GTAC'C Cuts at: 3488 3488 Size: 6163 MfeI C'AATT_G Cuts at: 3762 3762 Size: 6163 MluI A'CGCG_T Cuts at: 6039 6039 Size: 6163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3312 5277 5461 1305 Size: 2007 1965 184 2007 Fragments arranged by size: 2007 2007 1965 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2574 3434 10 Size: 54 1425 343 742 860 2739 Fragments arranged by size: 2739 1425 860 742 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4121 4480 4639 518 Size: 648 2955 359 159 2042 Fragments arranged by size: 2955 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2715 3268 3579 4256 5404 5649 1606 Size: 1109 553 311 677 1148 245 2120 Fragments arranged by size: 2120 1148 1109 677 553 311 245 NaeI GCC'GGC Cuts at: 3070 3070 Size: 6163 NarI GG'CG_CC Cuts at: 2145 2388 2145 Size: 243 5920 NcoI C'CATG_G Cuts at: 513 1065 2949 513 Size: 552 1884 3727 Fragments arranged by size: 3727 1884 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6163 NgoMIV G'CCGG_C Cuts at: 3068 3068 Size: 6163 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6163 NotI GC'GGCC_GC Cuts at: 3610 3610 Size: 6163 NspI r_CATG'y Cuts at: 1132 1229 2482 2620 3521 5066 1132 Size: 97 1253 138 901 1545 2229 Fragments arranged by size: 2229 1545 1253 901 138 97 PasI CC'CwG_GG Cuts at: 1348 2212 2664 1348 Size: 864 452 4847 Fragments arranged by size: 4847 864 452 PciI A'CATG_T Cuts at: 5062 5062 Size: 6163 PflMI CCAn_nnn'nTGG Cuts at: 1108 2581 3357 1108 Size: 1473 776 3914 Fragments arranged by size: 3914 1473 776 PfoI T'CCnGG_A Cuts at: 3255 3255 Size: 6163 PmeI GTTT'AAAC Cuts at: 3467 3467 Size: 6163 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3236 3268 4232 4264 5745 5777 3236 Size: 32 964 32 1481 32 3622 Fragments arranged by size: 3622 1481 964 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2027 2027 Size: 6163 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6163 PsiI TTA'TAA Cuts at: 3733 3733 Size: 6163 PspOMI G'GGCC_C Cuts at: 2235 2811 2235 Size: 576 5587 PspXI vC'TCGA_Gb Cuts at: 2021 2021 Size: 6163 PstI C_TGCA'G Cuts at: 830 2907 3515 830 Size: 2077 608 3478 Fragments arranged by size: 3478 2077 608 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6163 PvuII CAG'CTG Cuts at: 3268 3268 Size: 6163 SacI G_AGCT'C Cuts at: 2678 2678 Size: 6163 SalI G'TCGA_C Cuts at: 1936 3505 1936 Size: 1569 4594 SanDI GG'GwC_CC Cuts at: 2027 2027 Size: 6163 SapI GCTCTTCn'nnn_ Cuts at: 3147 3147 Size: 6163 SbfI CC_TGCA'GG Cuts at: 3515 3515 Size: 6163 ScaI AGT'ACT Cuts at: 4411 6123 4411 Size: 1712 4451 SfcI C'TryA_G Cuts at: 826 1045 2315 2903 3511 4646 5327 5518 826 Size: 219 1270 588 608 1135 681 191 1471 Fragments arranged by size: 1471 1270 1135 681 608 588 219 191 SfoI GGC'GCC Cuts at: 2146 2389 2146 Size: 243 5920 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6163 SmaI CCC'GGG Cuts at: 1872 2861 3490 1872 Size: 989 629 4545 Fragments arranged by size: 4545 989 629 SmlI C'TyrA_G Cuts at: 820 1017 1943 2021 4267 5168 5430 820 Size: 197 926 78 2246 901 262 1553 Fragments arranged by size: 2246 1553 926 901 262 197 78 SnaBI TAC'GTA Cuts at: 493 2606 3954 493 Size: 2113 1348 2702 Fragments arranged by size: 2702 2113 1348 SpeI A'CTAG_T Cuts at: 152 152 Size: 6163 SphI G_CATG'C Cuts at: 1132 3521 1132 Size: 2389 3774 SspI AAT'ATT Cuts at: 5 52 4087 5 Size: 47 4035 2081 Fragments arranged by size: 4035 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6163 StyI C'CwwG_G Cuts at: 513 1065 2949 3240 3602 513 Size: 552 1884 291 362 3074 Fragments arranged by size: 3074 1884 552 362 291 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2225 2660 4190 4207 4360 4545 4990 Size: 1255 645 435 1530 17 153 185 445 Cuts at: 4990 325 Size: 1498 Fragments arranged by size: 1530 1498 1255 645 445 435 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4409 6121 96 Size: 274 80 33 51 1443 2432 1712 138 Fragments arranged by size: 2432 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2164 2196 3331 3363 6123 6155 2164 Size: 32 1135 32 2760 32 2172 Fragments arranged by size: 2760 2172 1135 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3315 1316 Size: 1999 4164 XbaI T'CTAG_A Cuts at: 3499 3499 Size: 6163 XhoI C'TCGA_G Cuts at: 1943 2021 1943 Size: 78 6085 XmaI C'CCGG_G Cuts at: 1870 2859 3488 1870 Size: 989 629 4545 Fragments arranged by size: 4545 989 629 XmnI GAAnn'nnTTC Cuts at: 3156 4292 3156 Size: 1136 5027 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3971 276 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI