(Linear) (Six-base) MAPSORT of: KIEE1940.seq Check: 7630 from: 1 to: 1458 KIEE1940.orf With 182 enzymes: SgfI * July 15, 2010 18:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 655 1458 Size: 655 803 AccI GT'mk_AC Cuts at: 0 770 1458 Size: 770 688 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 928 1138 1173 1304 1458 Size: 928 210 35 131 154 Fragments arranged by size: 928 210 154 131 35 AflIII A'CryG_T Cuts at: 0 1091 1458 Size: 1091 367 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1231 1263 1458 Size: 1231 32 195 Fragments arranged by size: 1231 195 32 AlwNI CAG_nnn'CTG Cuts at: 0 213 793 906 1083 1274 1314 1458 Size: 213 580 113 177 191 40 144 Fragments arranged by size: 580 213 191 177 144 113 40 ApaI G_GGCC'C Cuts at: 0 234 810 1458 Size: 234 576 648 Fragments arranged by size: 648 576 234 ApoI r'AATT_y Cuts at: 0 1069 1458 Size: 1069 389 AvaI C'yCGr_G Cuts at: 0 16 854 1458 Size: 16 838 604 Fragments arranged by size: 838 604 16 BamHI G'GATC_C Cuts at: 0 1024 1458 Size: 1024 434 BanI G'GyrC_C Cuts at: 0 139 166 382 825 1458 Size: 139 27 216 443 633 Fragments arranged by size: 633 443 216 139 27 BanII G_rGCy'C Cuts at: 0 126 153 234 673 810 1458 Size: 126 27 81 439 137 648 Fragments arranged by size: 648 439 137 126 81 27 BbeI G_GCGC'C Cuts at: 0 143 386 1458 Size: 143 243 1072 Fragments arranged by size: 1072 243 143 BbsI GAAGACnn'nnnn_ Cuts at: 0 1094 1179 1458 Size: 1094 85 279 Fragments arranged by size: 1094 279 85 BglI GCCn_nnn'nGGC Cuts at: 0 380 1458 Size: 380 1078 BlpI GC'TnA_GC Cuts at: 0 1264 1458 Size: 1264 194 Bme1580I G_kGCm'C Cuts at: 0 169 234 810 1458 Size: 169 65 576 648 Fragments arranged by size: 648 576 169 65 BmgBI CAC'GTC Cuts at: 0 1092 1294 1458 Size: 1092 202 164 Fragments arranged by size: 1092 202 164 BmrI ACTGGGnnnn_n' Cuts at: 0 381 1405 1432 1458 Size: 381 1024 27 26 Fragments arranged by size: 1024 381 27 26 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 391 1458 Size: 391 1067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 469 517 1158 1458 Size: 64 405 48 641 300 Fragments arranged by size: 641 405 300 64 48 Bpu10I CC'TnA_GC Cuts at: 0 677 1458 Size: 677 781 BsaAI yAC'GTr Cuts at: 0 601 1458 Size: 601 857 BsaHI Gr'CG_yC Cuts at: 0 140 383 683 923 1458 Size: 140 243 300 240 535 Fragments arranged by size: 535 300 243 240 140 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 159 189 1231 1261 1458 Size: 159 30 1042 30 197 Fragments arranged by size: 1042 197 159 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1132 1458 Size: 1132 326 BseYI C'CCAG_C Cuts at: 0 135 199 615 1458 Size: 135 64 416 843 Fragments arranged by size: 843 416 135 64 BsiHKAI G_wGCw'C Cuts at: 0 673 876 1458 Size: 673 203 582 Fragments arranged by size: 673 582 203 Bsp1286I G_dGCh'C Cuts at: 0 126 153 169 234 673 810 876 1458 Size: 126 27 16 65 439 137 66 582 Fragments arranged by size: 582 439 137 126 66 65 27 16 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 281 282 332 655 1184 1458 Size: 281 1 50 323 529 274 Fragments arranged by size: 529 323 281 274 50 1 BsrBI CCG'CTC Cuts at: 0 252 1458 Size: 252 1206 BsrFI r'CCGG_y Cuts at: 0 1063 1458 Size: 1063 395 BssSI C'ACGA_G Cuts at: 0 253 376 1458 Size: 253 123 1082 Fragments arranged by size: 1082 253 123 BstAPI GCAn_nnn'nTGC Cuts at: 0 1274 1458 Size: 1274 184 BstXI CCAn_nnnn'nTGG Cuts at: 0 1376 1458 Size: 1376 82 BstYI r'GATC_y Cuts at: 0 1024 1458 Size: 1024 434 Bsu36I CC'TnA_GG Cuts at: 0 286 1076 1458 Size: 286 790 382 Fragments arranged by size: 790 382 286 BtgI C'CryG_G Cuts at: 0 944 1458 Size: 944 514 EaeI y'GGCC_r Cuts at: 0 261 342 567 1427 1458 Size: 261 81 225 860 31 Fragments arranged by size: 860 261 225 81 31 EarI CTCTTCn'nnn_ Cuts at: 0 1142 1458 Size: 1142 316 EcoICRI GAG'CTC Cuts at: 0 671 1458 Size: 671 787 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 469 517 928 1138 1158 1173 1304 Size: 64 405 48 411 210 20 15 131 Cuts at: 1304 1458 Size: 154 Fragments arranged by size: 411 405 210 154 131 64 48 20 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 289 1114 1458 Size: 289 825 344 Fragments arranged by size: 825 344 289 EcoO109I rG'GnC_Cy Cuts at: 0 22 112 210 231 807 1458 Size: 22 90 98 21 576 651 Fragments arranged by size: 651 576 98 90 22 21 FspI TGC'GCA Cuts at: 0 328 1458 Size: 328 1130 HaeII r_GCGC'y Cuts at: 0 143 386 1458 Size: 143 243 1072 Fragments arranged by size: 1072 243 143 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1296 1328 1458 Size: 1296 32 130 Fragments arranged by size: 1296 130 32 HincII GTy'rAC Cuts at: 0 321 1458 Size: 321 1137 HpaI GTT'AAC Cuts at: 0 321 1458 Size: 321 1137 KasI G'GCGC_C Cuts at: 0 139 382 1458 Size: 139 243 1076 Fragments arranged by size: 1076 243 139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1307 1458 Size: 1307 151 MscI TGG'CCA Cuts at: 0 569 1429 1458 Size: 569 860 29 Fragments arranged by size: 860 569 29 MspA1I CmG'CkG Cuts at: 0 710 1263 1458 Size: 710 553 195 Fragments arranged by size: 710 553 195 NaeI GCC'GGC Cuts at: 0 1065 1458 Size: 1065 393 NarI GG'CG_CC Cuts at: 0 140 383 1458 Size: 140 243 1075 Fragments arranged by size: 1075 243 140 NcoI C'CATG_G Cuts at: 0 944 1458 Size: 944 514 NgoMIV G'CCGG_C Cuts at: 0 1063 1458 Size: 1063 395 NspI r_CATG'y Cuts at: 0 477 615 1458 Size: 477 138 843 Fragments arranged by size: 843 477 138 PasI CC'CwG_GG Cuts at: 0 207 659 1458 Size: 207 452 799 Fragments arranged by size: 799 452 207 PflMI CCAn_nnn'nTGG Cuts at: 0 576 1352 1458 Size: 576 776 106 Fragments arranged by size: 776 576 106 PfoI T'CCnGG_A Cuts at: 0 1250 1458 Size: 1250 208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1231 1263 1458 Size: 1231 32 195 Fragments arranged by size: 1231 195 32 PpuMI rG'GwC_Cy Cuts at: 0 22 1458 Size: 22 1436 PspOMI G'GGCC_C Cuts at: 0 230 806 1458 Size: 230 576 652 Fragments arranged by size: 652 576 230 PspXI vC'TCGA_Gb Cuts at: 0 16 1458 Size: 16 1442 PstI C_TGCA'G Cuts at: 0 902 1458 Size: 902 556 PvuII CAG'CTG Cuts at: 0 1263 1458 Size: 1263 195 SacI G_AGCT'C Cuts at: 0 673 1458 Size: 673 785 SanDI GG'GwC_CC Cuts at: 0 22 1458 Size: 22 1436 SapI GCTCTTCn'nnn_ Cuts at: 0 1142 1458 Size: 1142 316 SfcI C'TryA_G Cuts at: 0 310 898 1458 Size: 310 588 560 Fragments arranged by size: 588 560 310 SfoI GGC'GCC Cuts at: 0 141 384 1458 Size: 141 243 1074 Fragments arranged by size: 1074 243 141 SmaI CCC'GGG Cuts at: 0 856 1458 Size: 856 602 SmlI C'TyrA_G Cuts at: 0 16 1458 Size: 16 1442 SnaBI TAC'GTA Cuts at: 0 601 1458 Size: 601 857 StyI C'CwwG_G Cuts at: 0 944 1235 1458 Size: 944 291 223 Fragments arranged by size: 944 291 223 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 220 655 1458 Size: 220 435 803 Fragments arranged by size: 803 435 220 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 159 191 1326 1358 1458 Size: 159 32 1135 32 100 Fragments arranged by size: 1135 159 100 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1310 1458 Size: 1310 148 XhoI C'TCGA_G Cuts at: 0 16 1458 Size: 16 1442 XmaI C'CCGG_G Cuts at: 0 854 1458 Size: 854 604 XmnI GAAnn'nnTTC Cuts at: 0 1151 1458 Size: 1151 307 Enzymes that do cut: AarI AccI AcuI AflIII AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII HpaI KasI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SanDI SapI SfcI SfoI SmaI SmlI SnaBI StyI TaqII TstI Tth111I XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII BmtI BpuEI BsaI BsaBI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI FspAI HindIII KpnI MfeI MluI MslI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SpeI SphI SrfI SspI StuI SwaI TatI XbaI XcmI ZraI