################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BpmI BpuAI BsaBI BsaHI BsaI BsiEI BsiHKAI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI BstAPI BstBI BstEII BstPI BstXI BstYI Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MboII MfeI MflI MluI MluNI MscI MseI MunI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StyI SwaI Tru9I Tsp509I Tth111I XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 738 GTATAC GT^[AC][GT]AC AccII 724 CGCG CG^CG AccIII 640 TCCGGA T^CCGGA AflIII 460 ACGTGT A^C[GA][CT]GT AlwI 767 GGATC GGATC(4/5) BbrPI 459 CACGTG CAC^GTG BfaI 332 CTAG C^TAG BglI 485 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 9 GCTCAGC GC^T[ACGT]AGC Bpu1102I 9 GCTCAGC GC^T[ACGT]AGC BsaAI 459 CACGTG [CT]AC^GT[GA] BsaWI 640 TCCGGA [AT]^CCGG[AT] BseAI 640 TCCGGA T^CCGGA BsgI 602 GTGCAG GTGCAG(16/14) BsmBI 309 CGTCTC CGTCTC(1/5) BsmFI 147 GTCCC GGGAC(10/14) BspEI 640 TCCGGA T^CCGGA BssHII 723 GCGCGC G^CGCGC BssSI 624 CTCGTG CACGAG(-5/-1) Bst1107I 738 GTATAC GTA^TAC BstF5I 428 CATCC GGATG(2/0) BstUI 724 CGCG CG^CG BstZ17I 738 GTATAC GTA^TAC CelII 9 GCTCAGC GC^T[ACGT]AGC DraII 645 AGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 240 TGGCCG [CT]^GGCC[GA] EcoO109I 645 AGGCCCT [GA]G^G[ACGT]CC[CT] FokI 428 CATCC GGATG(9/13) HgaI 366 GACGC GACGC(5/10) HincII 103 GTCAAC GT[CT]^[GA]AC HindII 103 GTCAAC GT[CT]^[GA]AC MaeI 332 CTAG C^TAG MroI 640 TCCGGA T^CCGGA MspA1I 162 CAGCGG C[AC]G^C[GT]G MvnI 724 CGCG CG^CG NaeI 560 GCCGGC GCC^GGC NgoMIV 560 GCCGGC G^CCGGC PaeR7I 743 CTCGAG C^TCGAG PflMI 470 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 459 CACGTG CAC^GTG PmlI 459 CACGTG CAC^GTG SbfI 150 CCTGCAGG CCTGCA^GG ScaI 566 AGTACT AGT^ACT SexAI 773 ACCAGGT A^CC[AT]GGT Sse8387I 150 CCTGCAGG CCTGCA^GG StuI 453 AGGCCT AGG^CCT TaqI 744 TCGA T^CGA TfiI 552 GAATC G^A[AT]TC TthHB8I 744 TCGA T^CGA Van91I 470 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 171 CCACTTCTGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 743 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 396 GTAC GT^AC 567 GTAC AlwNI 298 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG 759 CAGCACCTG AvaI 314 CTCGGG C^[CT]CG[GA]G 743 CTCGAG BanI 616 GGTGCC G^G[CT][GA]CC 703 GGCACC BsmAI 310 GTCTC GTCTC(1/5) 694 GTCTC BsmI 114 GAATGC GAATGC(1/-1) 59 GCATTC BsoBI 314 CTCGGG C^[CT]CG[GA]G 743 CTCGAG HphI 634 GGTGA GGTGA(8/7) 467 TCACC MaeII 362 ACGT A^CGT 460 ACGT MslI 223 CACTGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 672 CACATGGGTG PleI 589 GACTC GAGTC(4/5) 637 GACTC PstI 151 CTGCAG CTGCA^G 520 CTGCAG RsaI 396 GTAC GT^AC 567 GTAC SfaNI 407 GCATC GCATC(5/9) 427 GCATC SfcI 151 CTGCAG C^T[GA][CT]AG 520 CTGCAG SmlI 576 CTCAAG C^T[CT][GA]AG 743 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AluI BanII BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeIII MboI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I TspRI