(Circular) (Six-base) MAPSORT of: pFN21AE6265.seq Check: 8209 from: 1 to: 5497 pFN21AE6265 5497 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2137 2521 2600 2609 2743 2137 Size: 384 79 9 134 4891 Fragments arranged by size: 4891 384 134 79 9 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3307 278 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2744 2840 1937 Size: 807 96 4594 Fragments arranged by size: 4594 807 96 Acc65I G'GTAC_C Cuts at: 2818 2818 Size: 5497 AclI AA'CG_TT Cuts at: 1563 2796 3624 3997 1563 Size: 1233 828 373 3063 Fragments arranged by size: 3063 1233 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2077 2399 2475 3560 4944 5212 1632 Size: 445 322 76 1085 1384 268 1917 Fragments arranged by size: 1917 1384 1085 445 322 268 76 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5300 AflIII A'CryG_T Cuts at: 1203 2465 4396 5373 1203 Size: 1262 1931 977 1327 Fragments arranged by size: 1931 1327 1262 977 AgeI A'CCGG_T Cuts at: 4328 4328 Size: 5497 AhdI GACnn_n'nnGTC Cuts at: 4226 4226 Size: 5497 AleI CACnn'nnGTG Cuts at: 1166 2681 1166 Size: 1515 3982 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5497 AlwNI CAG_nnn'CTG Cuts at: 2308 2769 3240 4812 2308 Size: 461 471 1572 2993 Fragments arranged by size: 2993 1572 471 461 ApaLI G'TGCA_C Cuts at: 3554 4710 3554 Size: 1156 4341 ApoI r'AATT_y Cuts at: 1493 2806 3032 3184 5293 1493 Size: 1313 226 152 2109 1697 Fragments arranged by size: 2109 1697 1313 226 152 AseI AT'TA_AT Cuts at: 160 1032 4051 160 Size: 872 3019 1606 Fragments arranged by size: 3019 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 AvaI C'yCGr_G Cuts at: 1870 1943 2319 2748 2822 1870 Size: 73 376 429 74 4545 Fragments arranged by size: 4545 429 376 74 73 BamHI G'GATC_C Cuts at: 2827 3194 2827 Size: 367 5130 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2621 2708 2818 4273 618 Size: 325 217 627 834 87 110 1455 1842 Fragments arranged by size: 1842 1455 834 627 325 217 110 87 BanII G_rGCy'C Cuts at: 1391 2135 2298 2582 2816 1391 Size: 744 163 284 234 4072 Fragments arranged by size: 4072 744 284 234 163 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4526 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3688 3722 1903 Size: 34 1751 34 3678 Fragments arranged by size: 3678 1751 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3389 4599 3389 Size: 1210 4287 BclI T'GATC_A Cuts at: 1548 1548 Size: 5497 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2496 4108 136 Size: 107 122 71 631 605 824 1612 1525 Fragments arranged by size: 1612 1525 824 631 605 122 107 71 BglII A'GATC_T Cuts at: 5492 5492 Size: 5497 BlpI GC'TnA_GC Cuts at: 2015 2914 2015 Size: 899 4598 Bme1580I G_kGCm'C Cuts at: 1790 2711 3558 4714 1790 Size: 921 847 1156 2573 Fragments arranged by size: 2573 1156 921 847 BmgBI CAC'GTC Cuts at: 1318 2368 1318 Size: 1050 4447 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2392 2584 3228 Size: 416 53 83 399 24 975 192 644 Cuts at: 3228 4186 442 Size: 958 1753 Fragments arranged by size: 1753 975 958 644 416 399 192 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5497 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4157 1239 Size: 2918 2579 Bpu10I CC'TnA_GC Cuts at: 2027 2027 Size: 5497 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2566 3622 4487 4785 2566 Size: 1056 865 298 3278 Fragments arranged by size: 3278 1056 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4160 882 Size: 617 2661 2219 Fragments arranged by size: 2661 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2466 3288 493 Size: 711 1262 822 2702 Fragments arranged by size: 2702 1262 822 711 BsaBI GATnn'nnATC Cuts at: 5491 5491 Size: 5497 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3304 3686 4321 275 Size: 53 83 186 936 1771 382 635 1451 Fragments arranged by size: 1771 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2645 3930 4328 4602 4749 5160 2645 Size: 1285 398 274 147 411 2982 Fragments arranged by size: 2982 1285 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5272 Fragments arranged by size: 5272 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2038 2617 1186 Size: 844 8 579 4066 Fragments arranged by size: 4066 844 579 8 BseYI C'CCAG_C Cuts at: 2481 4700 5196 2481 Size: 2219 496 2782 Fragments arranged by size: 2782 2219 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2628 1163 Size: 573 892 4032 Fragments arranged by size: 4032 892 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2947 3708 4736 1540 Size: 275 186 946 761 1028 2301 Fragments arranged by size: 2301 1028 946 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3558 3643 4714 3558 Size: 85 1071 4341 Fragments arranged by size: 4341 1071 85 BsmI GAATG_Cn' Cuts at: 2064 2125 3008 3101 2064 Size: 61 883 93 4460 Fragments arranged by size: 4460 883 93 61 BsmBI CGTCTCn'nnnn_ Cuts at: 2320 2320 Size: 5497 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2298 2582 2711 2816 3558 3643 Size: 399 345 163 284 129 105 742 85 Cuts at: 3643 4714 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 345 284 163 129 105 85 BspEI T'CCGG_A Cuts at: 2645 5160 2645 Size: 2515 2982 BspHI T'CATG_A Cuts at: 3386 5116 3386 Size: 1730 3767 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2137 2293 2521 2600 2609 2743 2852 844 Size: 1293 156 228 79 9 134 109 3489 Fragments arranged by size: 3489 1293 228 156 134 109 79 9 BsrBI CCG'CTC Cuts at: 1394 1823 3384 1394 Size: 429 1561 3507 Fragments arranged by size: 3507 1561 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2239 2512 2728 3992 4166 65 Size: 1183 991 273 216 1264 174 1396 Fragments arranged by size: 1396 1264 1183 991 273 216 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2080 2477 2565 4141 4328 1700 Size: 110 270 397 88 1576 187 2869 Fragments arranged by size: 2869 1576 397 270 187 110 88 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5497 BssHII G'CGCG_C Cuts at: 1925 2728 1925 Size: 803 4694 BssSI C'ACGA_G Cuts at: 2629 3557 4569 2629 Size: 928 1012 3557 Fragments arranged by size: 3557 1012 928 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5497 BstBI TT'CG_AA Cuts at: 4301 4301 Size: 5497 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5497 BstYI r'GATC_y Cuts at: 1972 2827 3194 3579 3596 5048 5134 5146 5492 Size: 855 367 385 17 1452 86 12 346 Cuts at: 5492 1972 Size: 1977 Fragments arranged by size: 1977 1452 855 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2745 2745 Size: 5497 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4945 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4758 Fragments arranged by size: 4758 683 56 BtsI GCAGTG_nn' Cuts at: 2130 2217 2227 3013 3807 3827 2130 Size: 87 10 786 794 20 3800 Fragments arranged by size: 3800 794 786 87 20 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5497 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5462 DraI TTT'AAA Cuts at: 2801 2801 Size: 5497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4504 809 Size: 507 3188 1802 Fragments arranged by size: 3188 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2245 2944 3833 Size: 54 1425 50 275 18 415 699 889 Cuts at: 3833 5462 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 699 415 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2944 1537 Size: 275 1132 4090 Fragments arranged by size: 4090 1132 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3427 1353 Size: 2074 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4084 4458 4604 387 Size: 1064 138 2495 374 146 1280 Fragments arranged by size: 2495 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2077 2399 2475 3560 4157 4944 5212 Size: 393 445 322 76 1085 597 787 268 Cuts at: 5212 1239 Size: 1524 Fragments arranged by size: 1524 1085 787 597 445 393 322 268 76 EcoO109I rG'GnC_Cy Cuts at: 2651 2651 Size: 5497 EcoRI G'AATT_C Cuts at: 2806 3184 2806 Size: 378 5119 FspI TGC'GCA Cuts at: 4003 4003 Size: 5497 HaeII r_GCGC'y Cuts at: 1678 4644 1678 Size: 2966 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2267 2299 4138 4170 4212 Size: 32 135 32 695 32 1839 32 42 Cuts at: 4212 4244 1373 Size: 32 2626 Fragments arranged by size: 2626 1839 695 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2110 2841 3087 669 Size: 988 281 172 731 246 3079 Fragments arranged by size: 3079 988 731 281 246 172 HindIII A'AGCT_T Cuts at: 748 748 Size: 5497 HpaI GTT'AAC Cuts at: 3087 3087 Size: 5497 KpnI G_GTAC'C Cuts at: 2822 2822 Size: 5497 MfeI C'AATT_G Cuts at: 3096 3096 Size: 5497 MluI A'CGCG_T Cuts at: 5373 5373 Size: 5497 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4611 4795 1305 Size: 3306 184 2007 Fragments arranged by size: 3306 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3675 Fragments arranged by size: 3675 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2232 2681 3455 3814 3973 518 Size: 648 1066 449 774 359 159 2042 Fragments arranged by size: 2042 1066 774 648 449 359 159 MspA1I CmG'CkG Cuts at: 1606 2169 2913 3590 4738 4983 1606 Size: 563 744 677 1148 245 2120 Fragments arranged by size: 2120 1148 744 677 563 245 NaeI GCC'GGC Cuts at: 2567 2567 Size: 5497 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4945 NdeI CA'TA_TG Cuts at: 387 387 Size: 5497 NgoMIV G'CCGG_C Cuts at: 2565 2565 Size: 5497 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5497 NotI GC'GGCC_GC Cuts at: 2944 2944 Size: 5497 NspI r_CATG'y Cuts at: 1132 1229 2855 4400 1132 Size: 97 1626 1545 2229 Fragments arranged by size: 2229 1626 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5497 PciI A'CATG_T Cuts at: 4396 4396 Size: 5497 PflMI CCAn_nnn'nTGG Cuts at: 1108 2481 1108 Size: 1373 4124 PmeI GTTT'AAAC Cuts at: 2801 2801 Size: 5497 PmlI CAC'GTG Cuts at: 2466 2466 Size: 5497 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3566 3598 5079 5111 3566 Size: 32 1481 32 3952 Fragments arranged by size: 3952 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5497 PsiI TTA'TAA Cuts at: 3067 3067 Size: 5497 PspXI vC'TCGA_Gb Cuts at: 2748 2748 Size: 5497 PstI C_TGCA'G Cuts at: 830 2160 2529 2849 830 Size: 1330 369 320 3478 Fragments arranged by size: 3478 1330 369 320 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5497 SalI G'TCGA_C Cuts at: 1936 2839 1936 Size: 903 4594 SbfI CC_TGCA'GG Cuts at: 2160 2849 2160 Size: 689 4808 ScaI AGT'ACT Cuts at: 2573 3745 5457 2573 Size: 1172 1712 2613 Fragments arranged by size: 2613 1712 1172 SexAI A'CCwGG_T Cuts at: 2778 2778 Size: 5497 SfcI C'TryA_G Cuts at: 826 1045 2156 2525 2845 3980 4661 4852 826 Size: 219 1111 369 320 1135 681 191 1471 Fragments arranged by size: 1471 1135 1111 681 369 320 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5497 SmaI CCC'GGG Cuts at: 1872 2824 1872 Size: 952 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2581 2748 3601 4502 4764 820 Size: 197 926 638 167 853 901 262 1553 Fragments arranged by size: 1553 926 901 853 638 262 197 167 SnaBI TAC'GTA Cuts at: 493 3288 493 Size: 2795 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5497 SphI G_CATG'C Cuts at: 1132 2855 1132 Size: 1723 3774 SspI AAT'ATT Cuts at: 5 52 3421 5 Size: 47 3369 2081 Fragments arranged by size: 3369 2081 47 StuI AGG'CCT Cuts at: 1517 2460 1517 Size: 943 4554 StyI C'CwwG_G Cuts at: 513 1065 2936 513 Size: 552 1871 3074 Fragments arranged by size: 3074 1871 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2200 2292 2671 3524 3541 3694 3879 Size: 1255 620 92 379 853 17 153 185 Cuts at: 3879 4324 325 Size: 445 1498 Fragments arranged by size: 1498 1255 853 620 445 379 185 153 92 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2400 2571 3743 Size: 274 80 33 51 1443 423 171 1172 Cuts at: 3743 5455 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1172 423 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5457 5489 5457 Size: 32 5465 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5497 XbaI T'CTAG_A Cuts at: 2833 2833 Size: 5497 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2183 2183 Size: 5497 XhoI C'TCGA_G Cuts at: 1943 2748 1943 Size: 805 4692 XmaI C'CCGG_G Cuts at: 1870 2822 1870 Size: 952 4545 XmnI GAAnn'nnTTC Cuts at: 3626 3626 Size: 5497 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3305 276 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI