Restriction map for
pFN21AE5561
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRV EcoT22I FbaI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI PvuII RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AclI 707 AACGTT AA^CGTT
AflII 226 CTTAAG C^TTAAG
AspHI 1066 GAGCTC G[AT]GC[AT]^C
BciVI 540 GGATAC GTATCC(6/5)
BfaI 991 CTAG C^TAG
BfrI 226 CTTAAG C^TTAAG
BlpI 367 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 367 GCTGAGC GC^T[ACGT]AGC
BseRI 149 CTCCTC GAGGAG(10/8)
BsgI 1102 CTGCAC GTGCAG(16/14)
BsiHKAI 1066 GAGCTC G[AT]GC[AT]^C
BsmFI 1052 GGGAC GGGAC(10/14)
BsmI 65 GAATGC GAATGC(1/-1)
BstEII 1209 GGTGACC G^GT[ACGT]ACC
BstPI 1209 GGTGACC G^GT[ACGT]ACC
BstXI 327 CCAGTCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 983 AGATCC [GA]^GATC[CT]
CelII 367 GCTGAGC GC^T[ACGT]AGC
CfoI 338 GCGC GCG^C
EarI 376 CTCTTC CTCTTC(1/4)
EcoNI 177 CCTGACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1209 GGTGACC G^GT[ACGT]ACC
EcoRI 52 GAATTC G^AATTC
FauI 696 GCGGG CCCGC(4/6)
HhaI 338 GCGC GCG^C
HinP1I 338 GCGC G^CGC
Ksp632I 376 CTCTTC CTCTTC(1/4)
MaeI 991 CTAG C^TAG
MaeIII 1210 GTGAC ^GT[ACGT]AC
MflI 983 AGATCC [GA]^GATC[CT]
Psp1406I 707 AACGTT AA^CGTT
SacI 1066 GAGCTC GAGCT^C
SapI 375 GCTCTTC GCTCTTC(1/4)
SmlI 226 CTTAAG C^T[CT][GA]AG
SphI 749 GCATGC GCATG^C
SspI 269 AATATT AAT^ATT
StuI 1231 AGGCCT AGG^CCT
TfiI 727 GATTC G^A[AT]TC
Tsp45I 1210 GTGAC ^GT[GC]AC
XcmI 480 CCAGATACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 983 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 52 GAATTC [GA]^AATT[CT]
800 AAATTC
AfaI 357 GTAC GT^AC
513 GTAC
AlwI 984 GATCC GGATC(4/5)
191 GGATC
ApoI 52 GAATTC [GA]^AATT[CT]
800 AAATTC
BanII 448 GAGCCC G[GA]GC[CT]^C
1066 GAGCTC
BbvI 372 GCAGC GCAGC(8/12)
655 GCTGC
BsmAI 222 GAGAC GTCTC(1/5)
147 GTCTC
Bsp1407I 356 TGTACA T^GTACA
512 TGTACA
BsrGI 356 TGTACA T^GTACA
512 TGTACA
BsrI 622 ACTGG ACTGG(1/-1)
327 CCAGT
DraIII 904 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1078 CACCCTGTG
DsaI 462 CCATGG C^C[GA][CT]GG
489 CCATGG
Fnu4HI 372 GCAGC GC^[ACGT]GC
655 GCTGC
ItaI 372 GCAGC GC^[ACGT]GC
655 GCTGC
MaeII 122 ACGT A^CGT
708 ACGT
MslI 833 CATCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
967 CATCGCTGTG
MspA1I 365 CCGCTG C[AC]G^C[GT]G
1253 CAGCGG
MwoI 60 GCTGGGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
749 GCATGCTTGGC
NcoI 462 CCATGG C^CATGG
489 CCATGG
NspI 749 GCATGC [GA]CATG^[CT]
1030 ACATGC
PflMI 462 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1077 CCACCCTGTGG
PleI 180 GACTC GAGTC(4/5)
421 GACTC
RsaI 357 GTAC GT^AC
513 GTAC
SbfI 534 CCTGCAGG CCTGCA^GG
628 CCTGCAGG
Sse8387I 534 CCTGCAGG CCTGCA^GG
628 CCTGCAGG
SspBI 356 TGTACA T^GTACA
512 TGTACA
TseI 372 GCAGC G^C[AT]GC
655 GCTGC
Van91I 462 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1077 CCACCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AvaII BbsI BmyI
BpmI BpuAI BsaJI BsiYI BslI
Bsp1286I BspMI BsrDI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I FokI HaeIII HinfI
HphI MboI MboII MnlI MseI
MvaI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I Tsp509I TspRI
TthHB8I