(Circular) (Six-base) MAPSORT of: pFN21AE5561.seq Check: 9231 from: 1 to: 5971 pFN21AE5561 5971 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 2713 4098 4471 1563 Size: 1150 1385 373 3063 Fragments arranged by size: 3063 1385 1150 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2084 2380 3152 4034 5418 5686 1632 Size: 452 296 772 882 1384 268 1917 Fragments arranged by size: 1917 1384 882 772 452 296 268 AflII C'TTAA_G Cuts at: 820 1017 2231 820 Size: 197 1214 4560 Fragments arranged by size: 4560 1214 197 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2555 1184 Size: 1371 4600 AlwNI CAG_nnn'CTG Cuts at: 3714 5286 3714 Size: 1572 4399 ApaLI G'TGCA_C Cuts at: 4028 5184 4028 Size: 1156 4815 ApoI r'AATT_y Cuts at: 1493 2057 2805 3280 3506 3658 5767 1493 Size: 564 748 475 226 152 2109 1697 Fragments arranged by size: 2109 1697 748 564 475 226 152 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 3296 1870 Size: 73 1353 4545 Fragments arranged by size: 4545 1353 73 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3292 4747 618 Size: 325 217 627 1505 1455 1842 Fragments arranged by size: 1842 1505 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2457 3075 3290 1391 Size: 1066 618 215 4072 Fragments arranged by size: 4072 1066 618 215 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2770 2799 3108 3158 928 Size: 971 871 29 309 50 3741 Fragments arranged by size: 3741 971 871 309 50 29 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4162 4196 1903 Size: 34 50 34 2141 34 3678 Fragments arranged by size: 3678 2141 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2539 3863 5073 2539 Size: 1324 1210 3437 Fragments arranged by size: 3437 1324 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 2373 3388 2373 Size: 1015 4956 Bme1580I G_kGCm'C Cuts at: 1790 2028 4032 5188 1790 Size: 238 2004 1156 2573 Fragments arranged by size: 2573 2004 1156 238 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3702 4660 442 Size: 416 53 83 399 24 2285 958 1753 Fragments arranged by size: 2285 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2376 2468 4631 1239 Size: 797 340 92 2163 2579 Fragments arranged by size: 2579 2163 797 340 92 Bpu10I CC'TnA_GC Cuts at: 2082 2082 Size: 5971 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4096 4961 5259 4096 Size: 865 298 4808 Fragments arranged by size: 4808 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4634 882 Size: 617 3135 2219 Fragments arranged by size: 3135 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3762 493 Size: 711 2558 2702 Fragments arranged by size: 2702 2558 711 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3778 4160 4795 275 Size: 53 83 186 936 2245 382 635 1451 Fragments arranged by size: 2245 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2425 2455 2490 2520 2539 Size: 30 165 30 1022 30 35 30 19 Cuts at: 2539 2569 1178 Size: 30 4580 Fragments arranged by size: 4580 1022 165 35 30 30 30 30 30 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2145 1186 Size: 959 5012 BseYI C'CCAG_C Cuts at: 2065 5174 5670 2065 Size: 3109 496 2366 Fragments arranged by size: 3109 2366 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3092 1163 Size: 573 1356 4042 Fragments arranged by size: 4042 1356 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3075 4032 4117 5188 3075 Size: 957 85 1071 3858 Fragments arranged by size: 3858 1071 957 85 BsmI GAATG_Cn' Cuts at: 2076 3482 3575 2076 Size: 1406 93 4472 Fragments arranged by size: 4472 1406 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2457 3075 3290 4032 4117 5188 Size: 399 238 429 618 215 742 85 1071 Cuts at: 5188 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 618 429 399 238 215 85 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2627 2641 2654 3326 844 Size: 1783 14 13 672 3489 Fragments arranged by size: 3489 1783 672 14 13 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2057 2593 3204 4466 4640 65 Size: 1183 809 536 611 1262 174 1396 Fragments arranged by size: 1396 1262 1183 809 611 536 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4615 4802 1700 Size: 110 2805 187 2869 Fragments arranged by size: 2869 2805 187 110 BsrGI T'GTAC_A Cuts at: 96 2361 2517 96 Size: 2265 156 3550 Fragments arranged by size: 3550 2265 156 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5971 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 3214 1184 Size: 2030 3941 BstXI CCAn_nnnn'nTGG Cuts at: 2339 2339 Size: 5971 BstYI r'GATC_y Cuts at: 1972 2988 3301 3668 4053 4070 5522 5608 5620 Size: 1016 313 367 385 17 1452 86 12 Cuts at: 5620 5966 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1016 385 367 346 313 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2467 2494 513 Size: 552 1402 27 3990 Fragments arranged by size: 3990 1402 552 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2957 487 Size: 683 56 1731 3501 Fragments arranged by size: 3501 1731 683 56 BtsI GCAGTG_nn' Cuts at: 3487 4281 4301 3487 Size: 794 20 5157 Fragments arranged by size: 5157 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DraIII CAC_nnn'GTG Cuts at: 2914 3088 2914 Size: 174 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936 Size: 54 1425 50 275 18 1588 889 1629 Cuts at: 5936 8 Size: 43 Fragments arranged by size: 1629 1588 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2387 3901 1353 Size: 1034 1514 3423 Fragments arranged by size: 3423 1514 1034 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4558 4932 5078 387 Size: 1064 138 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3073 3073 Size: 5971 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2084 2376 2380 2468 3152 4034 Size: 393 404 48 292 4 88 684 882 Cuts at: 4034 4631 5418 5686 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 882 787 684 597 404 393 292 268 88 48 4 EcoNI CCTnn'n_nnAGG Cuts at: 2186 2186 Size: 5971 EcoRI G'AATT_C Cuts at: 2057 3280 3658 2057 Size: 1223 378 4370 Fragments arranged by size: 4370 1223 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2765 2765 Size: 5971 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 5118 1678 Size: 3440 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2425 2457 2871 2903 4612 Size: 32 135 32 853 32 414 32 1709 Cuts at: 4612 4644 4686 4718 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1709 853 414 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3315 3561 669 Size: 988 281 1377 246 3079 Fragments arranged by size: 3079 1377 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5085 5269 1305 Size: 3780 184 2007 Fragments arranged by size: 3780 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4149 Fragments arranged by size: 4149 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2842 2976 3929 4288 4447 518 Size: 648 1676 134 953 359 159 2042 Fragments arranged by size: 2042 1676 953 648 359 159 134 MspA1I CmG'CkG Cuts at: 1606 2372 3260 3387 4064 5212 5457 1606 Size: 766 888 127 677 1148 245 2120 Fragments arranged by size: 2120 1148 888 766 677 245 127 NcoI C'CATG_G Cuts at: 513 1065 2467 2494 513 Size: 552 1402 27 3990 Fragments arranged by size: 3990 1402 552 27 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 2758 3039 3329 4874 1132 Size: 97 1529 281 290 1545 2229 Fragments arranged by size: 2229 1545 1529 290 281 97 PasI CC'CwG_GG Cuts at: 1348 2945 1348 Size: 1597 4374 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 2473 3088 1108 Size: 1365 615 3991 Fragments arranged by size: 3991 1365 615 PfoI T'CCnGG_A Cuts at: 2353 3066 2353 Size: 713 5258 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 2955 3541 2955 Size: 586 5385 PstI C_TGCA'G Cuts at: 830 2544 2638 2665 3323 830 Size: 1714 94 27 658 3478 Fragments arranged by size: 3478 1714 658 94 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 SacI G_AGCT'C Cuts at: 3075 3075 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SapI GCTCTTCn'nnn_ Cuts at: 2387 2387 Size: 5971 SbfI CC_TGCA'GG Cuts at: 2544 2638 3323 2544 Size: 94 685 5192 Fragments arranged by size: 5192 685 94 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SfcI C'TryA_G Cuts at: 826 1045 2140 2524 2540 2634 2661 3046 3319 Size: 219 1095 384 16 94 27 385 273 Cuts at: 3319 4454 5135 5326 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1095 681 385 384 273 219 191 94 27 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2231 4075 4976 5238 820 Size: 197 926 288 1844 901 262 1553 Fragments arranged by size: 1844 1553 926 901 288 262 197 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 2758 3329 1132 Size: 1626 571 3774 Fragments arranged by size: 3774 1626 571 SspI AAT'ATT Cuts at: 5 52 2276 3895 5 Size: 47 2224 1619 2081 Fragments arranged by size: 2224 2081 1619 47 StuI AGG'CCT Cuts at: 1517 3238 1517 Size: 1721 4250 StyI C'CwwG_G Cuts at: 513 1065 2467 2494 2550 3410 513 Size: 552 1402 27 56 860 3074 Fragments arranged by size: 3074 1402 860 552 56 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3998 4015 4168 4353 4798 325 Size: 1255 2418 17 153 185 445 1498 Fragments arranged by size: 2418 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2361 2517 4217 Size: 274 80 33 51 1443 384 156 1700 Cuts at: 4217 5929 96 Size: 1712 138 Fragments arranged by size: 1712 1700 1443 384 274 156 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3101 3133 5931 5963 3101 Size: 32 2798 32 3109 Fragments arranged by size: 3109 2798 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2492 2011 Size: 481 5490 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 4100 4100 Size: 5971 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI