(Linear) (Six-base) MAPSORT of: KIEE5561.seq Check: 9254 from: 1 to: 1266 KIEE5561.orf With 182 enzymes: SgfI * January 29, 2014 12:05 .. AclI AA'CG_TT Cuts at: 0 708 1266 Size: 708 558 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 375 1147 1266 Size: 79 296 772 119 Fragments arranged by size: 772 296 119 79 AflII C'TTAA_G Cuts at: 0 226 1266 Size: 226 1040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 550 1266 Size: 550 716 ApoI r'AATT_y Cuts at: 0 52 800 1266 Size: 52 748 466 Fragments arranged by size: 748 466 52 BanII G_rGCy'C Cuts at: 0 452 1070 1266 Size: 452 618 196 Fragments arranged by size: 618 452 196 BbsI GAAGACnn'nnnn_ Cuts at: 0 765 794 1103 1153 1266 Size: 765 29 309 50 113 Fragments arranged by size: 765 309 113 50 29 BciVI GTATCCnnnnn_n' Cuts at: 0 534 1266 Size: 534 732 BlpI GC'TnA_GC Cuts at: 0 368 1266 Size: 368 898 Bme1580I G_kGCm'C Cuts at: 0 23 1266 Size: 23 1243 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 371 463 1266 Size: 31 340 92 803 Fragments arranged by size: 803 340 92 31 Bpu10I CC'TnA_GC Cuts at: 0 77 1266 Size: 77 1189 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 420 450 485 515 534 564 1266 Size: 420 30 35 30 19 30 702 Fragments arranged by size: 702 420 35 30 30 30 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 140 1266 Size: 140 1126 BseYI C'CCAG_C Cuts at: 0 60 1266 Size: 60 1206 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1087 1266 Size: 1087 179 BsiHKAI G_wGCw'C Cuts at: 0 1070 1266 Size: 1070 196 BsmI GAATG_Cn' Cuts at: 0 71 1266 Size: 71 1195 Bsp1286I G_dGCh'C Cuts at: 0 23 452 1070 1266 Size: 23 429 618 196 Fragments arranged by size: 618 429 196 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 622 636 649 1266 Size: 622 14 13 617 Fragments arranged by size: 622 617 14 13 BsrDI GCAATG_nn' Cuts at: 0 52 588 1199 1266 Size: 52 536 611 67 Fragments arranged by size: 611 536 67 52 BsrGI T'GTAC_A Cuts at: 0 356 512 1266 Size: 356 156 754 Fragments arranged by size: 754 356 156 BstEII G'GTnAC_C Cuts at: 0 1209 1266 Size: 1209 57 BstXI CCAn_nnnn'nTGG Cuts at: 0 334 1266 Size: 334 932 BstYI r'GATC_y Cuts at: 0 983 1266 Size: 983 283 BtgI C'CryG_G Cuts at: 0 462 489 1266 Size: 462 27 777 Fragments arranged by size: 777 462 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 952 1266 Size: 952 314 DraIII CAC_nnn'GTG Cuts at: 0 909 1083 1266 Size: 909 174 183 Fragments arranged by size: 909 183 174 EarI CTCTTCn'nnn_ Cuts at: 0 382 1266 Size: 382 884 EcoICRI GAG'CTC Cuts at: 0 1068 1266 Size: 1068 198 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 79 371 375 463 1147 1266 Size: 31 48 292 4 88 684 119 Fragments arranged by size: 684 292 119 88 48 31 4 EcoNI CCTnn'n_nnAGG Cuts at: 0 181 1266 Size: 181 1085 EcoRI G'AATT_C Cuts at: 0 52 1266 Size: 52 1214 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 760 1266 Size: 760 506 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 420 452 866 898 1266 Size: 420 32 414 32 368 Fragments arranged by size: 420 414 368 32 32 MslI CAynn'nnrTG Cuts at: 0 837 971 1266 Size: 837 134 295 Fragments arranged by size: 837 295 134 MspA1I CmG'CkG Cuts at: 0 367 1255 1266 Size: 367 888 11 Fragments arranged by size: 888 367 11 NcoI C'CATG_G Cuts at: 0 462 489 1266 Size: 462 27 777 Fragments arranged by size: 777 462 27 NspI r_CATG'y Cuts at: 0 753 1034 1266 Size: 753 281 232 Fragments arranged by size: 753 281 232 PasI CC'CwG_GG Cuts at: 0 940 1266 Size: 940 326 PflMI CCAn_nnn'nTGG Cuts at: 0 468 1083 1266 Size: 468 615 183 Fragments arranged by size: 615 468 183 PfoI T'CCnGG_A Cuts at: 0 348 1061 1266 Size: 348 713 205 Fragments arranged by size: 713 348 205 PsiI TTA'TAA Cuts at: 0 950 1266 Size: 950 316 PstI C_TGCA'G Cuts at: 0 539 633 660 1266 Size: 539 94 27 606 Fragments arranged by size: 606 539 94 27 SacI G_AGCT'C Cuts at: 0 1070 1266 Size: 1070 196 SapI GCTCTTCn'nnn_ Cuts at: 0 382 1266 Size: 382 884 SbfI CC_TGCA'GG Cuts at: 0 539 633 1266 Size: 539 94 633 Fragments arranged by size: 633 539 94 SfcI C'TryA_G Cuts at: 0 135 519 535 629 656 1041 1266 Size: 135 384 16 94 27 385 225 Fragments arranged by size: 385 384 225 135 94 27 16 SmlI C'TyrA_G Cuts at: 0 226 1266 Size: 226 1040 SphI G_CATG'C Cuts at: 0 753 1266 Size: 753 513 SspI AAT'ATT Cuts at: 0 271 1266 Size: 271 995 StuI AGG'CCT Cuts at: 0 1233 1266 Size: 1233 33 StyI C'CwwG_G Cuts at: 0 462 489 545 1266 Size: 462 27 56 721 Fragments arranged by size: 721 462 56 27 TatI w'GTAC_w Cuts at: 0 356 512 1266 Size: 356 156 754 Fragments arranged by size: 754 356 156 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1096 1128 1266 Size: 1096 32 138 Fragments arranged by size: 1096 138 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 487 1266 Size: 487 779 Enzymes that do cut: AclI AcuI AflII AlfI ApoI BanII BbsI BciVI BlpI Bme1580I BpmI Bpu10I BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BstEII BstXI BstYI BtgI BtgZI DraIII EarI EcoICRI Eco57MI EcoNI EcoRI FalI Hin4I MslI MspA1I NcoI NspI PasI PflMI PfoI PsiI PstI SacI SapI SbfI SfcI SmlI SphI SspI StuI StyI TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EaeI EagI EciI EcoO109I EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI