Restriction map for
pFN21AE3074
##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaII AviII AvrII BalI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsmFI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DraIII DrdI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FspI
HindIII HpaI KpnI Ksp632I MaeI
MamI MboII MfeI MluI MluNI
MroI MscI MunI NciI NcoI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StyI
SwaI Tth111I XbaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AlwNI 610 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 385 AGCGCT AGC^GCT
Asp700I 498 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 335 GGATCC G^GATCC
BanI 64 GGCGCC G^G[CT][GA]CC
BanII 566 GAGCCC G[GA]GC[CT]^C
BbeI 64 GGCGCC GGCGC^C
BsaI 217 GAGACC GGTCTC(1/5)
BseRI 13 GAGGAG GAGGAG(10/8)
BsiEI 162 CGGCCG CG[GA][CT]^CG
BsmI 202 GCATTC GAATGC(1/-1)
BsrBI 6 GAGCGG CCGCTC(-3/-3)
BssHII 155 GCGCGC G^CGCGC
BstAPI 445 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 581 CCAGGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 192 GGCCC G^G[ACGT]CC
DraI 426 TTTAAA TTT^AAA
DsaI 111 CCGCGG C^C[GA][CT]GG
EagI 162 CGGCCG C^GGCCG
EclXI 162 CGGCCG C^GGCCG
Eco47III 385 AGCGCT AGC^GCT
Eco52I 162 CGGCCG C^GGCCG
FseI 120 GGCCGGCC GGCCGG^CC
HincII 370 GTCAAC GT[CT]^[GA]AC
HindII 370 GTCAAC GT[CT]^[GA]AC
KasI 64 GGCGCC G^GCGCC
KspI 111 CCGCGG CCGC^GG
MaeII 641 ACGT A^CGT
MseI 427 TTAA T^TAA
MslI 48 CACCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 111 CCGCGG C[AC]G^C[GT]G
NaeI 121 GCCGGC GCC^GGC
NarI 64 GGCGCC GG^CGCC
NdeI 488 CATATG CA^TATG
NgoMIV 121 GCCGGC G^CCGGC
NspI 229 GCATGC [GA]CATG^[CT]
PaeR7I 42 CTCGAG C^TCGAG
PflMI 609 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 291 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 273 CTGCAG CTGCA^G
SacII 111 CCGCGG CCGC^GG
Sau96I 192 GGCCC G^G[ACGT]CC
SfcI 273 CTGCAG C^T[GA][CT]AG
SfoI 64 GGCGCC GGC^GCC
SphI 229 GCATGC GCATG^C
Tru9I 427 TTAA T^TAA
Tsp45I 289 GTGAC ^GT[GC]AC
Tsp509I 450 AATT ^AATT
Van91I 609 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 42 CTCGAG C^TCGAG
XmnI 498 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 64 GGCGCC G[GA]^CG[CT]C
295 GGCGTC
AfaI 78 GTAC GT^AC
415 GTAC
AluI 17 AGCT AG^CT
271 AGCT
AspHI 45 GAGCAC G[AT]GC[AT]^C
546 GAGCAC
AvaI 42 CTCGAG C^[CT]CG[GA]G
60 CTCGGG
BsaHI 64 GGCGCC G[GA]^CG[CT]C
295 GGCGTC
BsaJI 111 CCGCGG C^C[ACGT][ACGT]GG
581 CCAGGG
BsgI 325 CTGCAC GTGCAG(16/14)
514 GTGCAG
BsiHKAI 45 GAGCAC G[AT]GC[AT]^C
546 GAGCAC
BsmAI 217 GAGAC GTCTC(1/5)
317 GTCTC
BsoBI 42 CTCGAG C^[CT]CG[GA]G
60 CTCGGG
BsrFI 96 GCCGGT [GA]^CCGG[CT]
121 GCCGGC
BstF5I 561 CATCC GGATG(2/0)
585 GGATG
BstYI 335 GGATCC [GA]^GATC[CT]
605 AGATCC
Cfr10I 96 GCCGGT [GA]^CCGG[CT]
121 GCCGGC
EaeI 119 TGGCCG [CT]^GGCC[GA]
162 CGGCCG
FauI 569 CCCGC CCCGC(4/6)
113 GCGGG
FokI 561 CATCC GGATG(9/13)
585 GGATG
HgaI 296 GCGTC GACGC(5/10)
533 GCGTC
Hin1I 64 GGCGCC G[GA]^CG[CT]C
295 GGCGTC
MaeIII 289 GTGAC ^GT[ACGT]AC
643 GTAAC
MflI 335 GGATCC [GA]^GATC[CT]
605 AGATCC
NlaIV 64 GGCGCC GG[ACGT]^[ACGT]CC
335 GGATCC
RsaI 78 GTAC GT^AC
415 GTAC
SmlI 42 CTCGAG C^T[CT][GA]AG
169 CTCAAG
StuI 38 AGGCCT AGG^CCT
246 AGGCCT
TaqI 29 TCGA T^CGA
43 TCGA
TfiI 354 GAATC G^A[AT]TC
684 GAATC
TthHB8I 29 TCGA T^CGA
43 TCGA
XhoII 335 GGATCC [GA]^GATC[CT]
605 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AlwI BbvI BmyI
BsiYI BslI Bsp1286I BspMI BsrI
BssKI BstNI BstUI Cac8I CfoI
DdeI DpnII EcoRII Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MnlI
MspI MvaI MvnI MwoI NdeII
NlaIII PleI Sau3AI ScrFI TseI
TspRI