(Circular) (Six-base) MAPSORT of: pFN21AE3074.seq Check: 984 from: 1 to: 5407 pFN21AE3074 5407 bp With 182 enzymes: SgfI * March 28, 2012 12:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3217 278 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2750 1937 Size: 813 4594 Acc65I G'GTAC_C Cuts at: 2728 2728 Size: 5407 AclI AA'CG_TT Cuts at: 1563 3534 3907 1563 Size: 1971 373 3063 Fragments arranged by size: 3063 1971 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3470 4854 5122 1632 Size: 1838 1384 268 1917 Fragments arranged by size: 1917 1838 1384 268 AfeI AGC'GCT Cuts at: 2392 2392 Size: 5407 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5210 AflIII A'CryG_T Cuts at: 1203 4306 5283 1203 Size: 3103 977 1327 Fragments arranged by size: 3103 1327 977 AgeI A'CCGG_T Cuts at: 4238 4238 Size: 5407 AhdI GACnn_n'nnGTC Cuts at: 4136 4136 Size: 5407 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5407 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2230 1184 Size: 1046 4361 AlwNI CAG_nnn'CTG Cuts at: 2620 3150 4722 2620 Size: 530 1572 3305 Fragments arranged by size: 3305 1572 530 ApaLI G'TGCA_C Cuts at: 3464 4620 3464 Size: 1156 4251 ApoI r'AATT_y Cuts at: 1493 2716 2942 3094 5203 1493 Size: 1223 226 152 2109 1697 Fragments arranged by size: 2109 1697 1223 226 152 AseI AT'TA_AT Cuts at: 160 1032 3961 160 Size: 872 2929 1606 Fragments arranged by size: 2929 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2065 2732 1870 Size: 73 104 18 667 4545 Fragments arranged by size: 4545 667 104 73 18 BamHI G'GATC_C Cuts at: 2340 2737 3104 2340 Size: 397 367 4643 Fragments arranged by size: 4643 397 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2728 4183 618 Size: 325 217 627 282 659 1455 1842 Fragments arranged by size: 1842 1455 659 627 325 282 217 BanII G_rGCy'C Cuts at: 1391 2575 2726 1391 Size: 1184 151 4072 Fragments arranged by size: 4072 1184 151 BbeI G_GCGC'C Cuts at: 2073 2073 Size: 5407 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4436 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3598 3632 1903 Size: 34 50 34 1577 34 3678 Fragments arranged by size: 3678 1577 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3299 4509 3299 Size: 1210 4197 BclI T'GATC_A Cuts at: 1548 1548 Size: 5407 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4018 136 Size: 107 122 71 631 605 2346 1525 Fragments arranged by size: 2346 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5402 5402 Size: 5407 BlpI GC'TnA_GC Cuts at: 2824 2824 Size: 5407 Bme1580I G_kGCm'C Cuts at: 1790 3468 4624 1790 Size: 1678 1156 2573 Fragments arranged by size: 2573 1678 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5407 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3138 4096 442 Size: 416 53 83 399 24 1721 958 1753 Fragments arranged by size: 1753 1721 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5407 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4067 1239 Size: 2828 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2159 3532 4397 4695 2159 Size: 1373 865 298 2871 Fragments arranged by size: 2871 1373 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2216 4070 882 Size: 617 717 1854 2219 Fragments arranged by size: 2219 1854 717 617 BsaAI yAC'GTr Cuts at: 493 1204 3198 493 Size: 711 1994 2702 Fragments arranged by size: 2702 1994 711 BsaBI GATnn'nnATC Cuts at: 5401 5401 Size: 5407 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 2301 3214 3596 Size: 53 83 186 936 537 231 913 382 Cuts at: 3596 4231 275 Size: 635 1451 Fragments arranged by size: 1451 936 913 635 537 382 231 186 83 53 BsaWI w'CCGG_w Cuts at: 3840 4238 4512 4659 5070 3840 Size: 398 274 147 411 4177 Fragments arranged by size: 4177 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5182 Fragments arranged by size: 5182 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 1186 Size: 847 4560 BseYI C'CCAG_C Cuts at: 4610 5106 4610 Size: 496 4911 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2315 2540 1163 Size: 573 579 225 4030 Fragments arranged by size: 4030 579 573 225 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2170 2857 3618 4646 1540 Size: 275 186 169 687 761 1028 2301 Fragments arranged by size: 2301 1028 761 687 275 186 169 BsiHKAI G_wGCw'C Cuts at: 2054 2555 3468 3553 4624 2054 Size: 501 913 85 1071 2837 Fragments arranged by size: 2837 1071 913 501 85 BsmI GAATG_Cn' Cuts at: 2207 2918 3011 2207 Size: 711 93 4603 Fragments arranged by size: 4603 711 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2555 2575 2726 3468 3553 4624 Size: 399 264 501 20 151 742 85 1071 Cuts at: 4624 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 501 399 264 151 85 20 BspEI T'CCGG_A Cuts at: 5070 5070 Size: 5407 BspHI T'CATG_A Cuts at: 3296 5026 3296 Size: 1730 3677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2160 2337 2685 2762 844 Size: 1316 177 348 77 3489 Fragments arranged by size: 3489 1316 348 177 77 BsrBI CCG'CTC Cuts at: 1394 1823 2013 3294 1394 Size: 429 190 1281 3507 Fragments arranged by size: 3507 1281 429 190 BsrDI GCAATG_nn' Cuts at: 65 1248 3902 4076 65 Size: 1183 2654 174 1396 Fragments arranged by size: 2654 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2101 2126 4051 4238 1700 Size: 110 291 25 1925 187 2869 Fragments arranged by size: 2869 1925 291 187 110 25 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5407 BssHII G'CGCG_C Cuts at: 1925 2160 1925 Size: 235 5172 BssSI C'ACGA_G Cuts at: 3467 4479 3467 Size: 1012 4395 BstAPI GCAn_nnn'nTGC Cuts at: 794 2456 794 Size: 1662 3745 BstBI TT'CG_AA Cuts at: 4211 4211 Size: 5407 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5407 BstXI CCAn_nnnn'nTGG Cuts at: 2593 2593 Size: 5407 BstYI r'GATC_y Cuts at: 1972 2340 2610 2737 3104 3489 3506 4958 5044 Size: 368 270 127 367 385 17 1452 86 Cuts at: 5044 5056 5402 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 368 367 346 270 127 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2116 513 Size: 552 1051 3804 Fragments arranged by size: 3804 1051 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4668 Fragments arranged by size: 4668 683 56 BtsI GCAGTG_nn' Cuts at: 2287 2505 2523 2923 3717 3737 2287 Size: 218 18 400 794 20 3957 Fragments arranged by size: 3957 794 400 218 20 18 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5407 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5372 DraI TTT'AAA Cuts at: 2433 2711 2433 Size: 278 5129 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4414 809 Size: 507 3098 1802 Fragments arranged by size: 3098 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2167 2854 Size: 54 1425 50 275 18 294 43 687 Cuts at: 2854 3743 5372 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 687 294 275 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2167 2854 1537 Size: 275 355 687 4090 Fragments arranged by size: 4090 687 355 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3337 1353 Size: 1984 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2531 3994 4368 4514 387 Size: 1064 138 942 1463 374 146 1280 Fragments arranged by size: 1463 1280 1064 942 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3470 4067 4854 5122 1239 Size: 393 1838 597 787 268 1524 Fragments arranged by size: 1838 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2716 3094 2716 Size: 378 5029 FseI GG_CCGG'CC Cuts at: 2130 2130 Size: 5407 FspI TGC'GCA Cuts at: 3913 3913 Size: 5407 HaeII r_GCGC'y Cuts at: 1678 2073 2116 2394 2620 4554 1678 Size: 395 43 278 226 1934 2531 Fragments arranged by size: 2531 1934 395 278 226 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2523 2555 2674 2706 4048 Size: 32 135 32 951 32 119 32 1342 Cuts at: 4048 4080 4122 4154 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1342 951 135 119 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2377 2751 2997 669 Size: 988 281 439 374 246 3079 Fragments arranged by size: 3079 988 439 374 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5407 HpaI GTT'AAC Cuts at: 2997 2997 Size: 5407 KasI G'GCGC_C Cuts at: 2069 2069 Size: 5407 KpnI G_GTAC'C Cuts at: 2732 2732 Size: 5407 MfeI C'AATT_G Cuts at: 3006 3006 Size: 5407 MluI A'CGCG_T Cuts at: 5283 5283 Size: 5407 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4521 4705 1305 Size: 3216 184 2007 Fragments arranged by size: 3216 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3585 Fragments arranged by size: 3585 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 3365 3724 3883 518 Size: 648 891 1308 359 159 2042 Fragments arranged by size: 2042 1308 891 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2118 2823 3500 4648 4893 1606 Size: 512 705 677 1148 245 2120 Fragments arranged by size: 2120 1148 705 677 512 245 NaeI GCC'GGC Cuts at: 2128 2128 Size: 5407 NarI GG'CG_CC Cuts at: 2070 2070 Size: 5407 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4855 NdeI CA'TA_TG Cuts at: 387 2494 387 Size: 2107 3300 NgoMIV G'CCGG_C Cuts at: 2126 2126 Size: 5407 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5407 NotI GC'GGCC_GC Cuts at: 2854 2854 Size: 5407 NspI r_CATG'y Cuts at: 1132 1229 2238 2765 4310 1132 Size: 97 1009 527 1545 2229 Fragments arranged by size: 2229 1545 1009 527 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5407 PciI A'CATG_T Cuts at: 4306 4306 Size: 5407 PflMI CCAn_nnn'nTGG Cuts at: 1108 2620 1108 Size: 1512 3895 PmeI GTTT'AAAC Cuts at: 2711 2711 Size: 5407 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3476 3508 4989 5021 3476 Size: 32 1481 32 3862 Fragments arranged by size: 3862 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2300 1613 Size: 687 4720 PsiI TTA'TAA Cuts at: 2977 2977 Size: 5407 PspXI vC'TCGA_Gb Cuts at: 2047 2047 Size: 5407 PstI C_TGCA'G Cuts at: 830 2282 2759 830 Size: 1452 477 3478 Fragments arranged by size: 3478 1452 477 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5407 SacII CC_GC'GG Cuts at: 2119 2119 Size: 5407 SalI G'TCGA_C Cuts at: 1936 2749 1936 Size: 813 4594 SbfI CC_TGCA'GG Cuts at: 2759 2759 Size: 5407 ScaI AGT'ACT Cuts at: 3655 5367 3655 Size: 1712 3695 SfcI C'TryA_G Cuts at: 826 1045 2278 2755 3890 4571 4762 826 Size: 219 1233 477 1135 681 191 1471 Fragments arranged by size: 1471 1233 1135 681 477 219 191 SfoI GGC'GCC Cuts at: 2071 2071 Size: 5407 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5407 SmaI CCC'GGG Cuts at: 1872 2734 1872 Size: 862 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2047 2174 3511 4412 4674 820 Size: 197 926 104 127 1337 901 262 1553 Fragments arranged by size: 1553 1337 926 901 262 197 127 104 SnaBI TAC'GTA Cuts at: 493 3198 493 Size: 2705 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5407 SphI G_CATG'C Cuts at: 1132 2238 2765 1132 Size: 1106 527 3774 Fragments arranged by size: 3774 1106 527 SspI AAT'ATT Cuts at: 5 52 3331 5 Size: 47 3279 2081 Fragments arranged by size: 3279 2081 47 StuI AGG'CCT Cuts at: 1517 2045 2253 1517 Size: 528 208 4671 Fragments arranged by size: 4671 528 208 StyI C'CwwG_G Cuts at: 513 1065 2846 513 Size: 552 1781 3074 Fragments arranged by size: 3074 1781 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2683 3434 3451 3604 3789 4234 325 Size: 1255 1103 751 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1103 751 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3653 5365 96 Size: 274 80 33 51 1443 1676 1712 138 Fragments arranged by size: 1712 1676 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5367 5399 5367 Size: 32 5375 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5407 XbaI T'CTAG_A Cuts at: 2743 2743 Size: 5407 XhoI C'TCGA_G Cuts at: 1943 2047 1943 Size: 104 5303 XmaI C'CCGG_G Cuts at: 1870 2732 1870 Size: 862 4545 XmnI GAAnn'nnTTC Cuts at: 2507 3536 2507 Size: 1029 4378 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3215 276 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI