(Linear) (Six-base) MAPSORT of: KIEE3074.seq Check: 8346 from: 1 to: 702 KIEE3074.orf With 182 enzymes: SgfI * March 22, 2012 16:10 .. AfeI AGC'GCT Cuts at: 0 387 702 Size: 387 315 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 225 702 Size: 225 477 AlwNI CAG_nnn'CTG Cuts at: 0 615 702 Size: 615 87 AvaI C'yCGr_G Cuts at: 0 42 60 702 Size: 42 18 642 Fragments arranged by size: 642 42 18 BamHI G'GATC_C Cuts at: 0 335 702 Size: 335 367 BanI G'GyrC_C Cuts at: 0 64 702 Size: 64 638 BanII G_rGCy'C Cuts at: 0 570 702 Size: 570 132 BbeI G_GCGC'C Cuts at: 0 68 702 Size: 68 634 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 702 Size: 154 548 BsaI GGTCTCn'nnnn_ Cuts at: 0 211 702 Size: 211 491 BsaHI Gr'CG_yC Cuts at: 0 65 296 702 Size: 65 231 406 Fragments arranged by size: 406 231 65 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 28 702 Size: 28 674 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 535 702 Size: 310 225 167 Fragments arranged by size: 310 225 167 BsiEI CG_ry'CG Cuts at: 0 165 702 Size: 165 537 BsiHKAI G_wGCw'C Cuts at: 0 49 550 702 Size: 49 501 152 Fragments arranged by size: 501 152 49 BsmI GAATG_Cn' Cuts at: 0 202 702 Size: 202 500 Bsp1286I G_dGCh'C Cuts at: 0 49 550 570 702 Size: 49 501 20 132 Fragments arranged by size: 501 132 49 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 155 332 680 702 Size: 155 177 348 22 Fragments arranged by size: 348 177 155 22 BsrBI CCG'CTC Cuts at: 0 8 702 Size: 8 694 BsrFI r'CCGG_y Cuts at: 0 96 121 702 Size: 96 25 581 Fragments arranged by size: 581 96 25 BssHII G'CGCG_C Cuts at: 0 155 702 Size: 155 547 BstAPI GCAn_nnn'nTGC Cuts at: 0 451 702 Size: 451 251 BstXI CCAn_nnnn'nTGG Cuts at: 0 588 702 Size: 588 114 BstYI r'GATC_y Cuts at: 0 335 605 702 Size: 335 270 97 Fragments arranged by size: 335 270 97 BtgI C'CryG_G Cuts at: 0 111 702 Size: 111 591 BtsI GCAGTG_nn' Cuts at: 0 282 500 518 702 Size: 282 218 18 184 Fragments arranged by size: 282 218 184 18 DraI TTT'AAA Cuts at: 0 428 702 Size: 428 274 EaeI y'GGCC_r Cuts at: 0 119 162 702 Size: 119 43 540 Fragments arranged by size: 540 119 43 EagI C'GGCC_G Cuts at: 0 162 702 Size: 162 540 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 526 702 Size: 526 176 FseI GG_CCGG'CC Cuts at: 0 125 702 Size: 125 577 HaeII r_GCGC'y Cuts at: 0 68 111 389 615 702 Size: 68 43 278 226 87 Fragments arranged by size: 278 226 87 68 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 518 550 669 701 702 Size: 518 32 119 32 1 Fragments arranged by size: 518 119 32 32 1 HincII GTy'rAC Cuts at: 0 372 702 Size: 372 330 KasI G'GCGC_C Cuts at: 0 64 702 Size: 64 638 MslI CAynn'nnrTG Cuts at: 0 52 702 Size: 52 650 MspA1I CmG'CkG Cuts at: 0 113 702 Size: 113 589 NaeI GCC'GGC Cuts at: 0 123 702 Size: 123 579 NarI GG'CG_CC Cuts at: 0 65 702 Size: 65 637 NdeI CA'TA_TG Cuts at: 0 489 702 Size: 489 213 NgoMIV G'CCGG_C Cuts at: 0 121 702 Size: 121 581 NspI r_CATG'y Cuts at: 0 233 702 Size: 233 469 PflMI CCAn_nnn'nTGG Cuts at: 0 615 702 Size: 615 87 PshAI GACnn'nnGTC Cuts at: 0 295 702 Size: 295 407 PspXI vC'TCGA_Gb Cuts at: 0 42 702 Size: 42 660 PstI C_TGCA'G Cuts at: 0 277 702 Size: 277 425 SacII CC_GC'GG Cuts at: 0 114 702 Size: 114 588 SfcI C'TryA_G Cuts at: 0 273 702 Size: 273 429 SfoI GGC'GCC Cuts at: 0 66 702 Size: 66 636 SmlI C'TyrA_G Cuts at: 0 42 169 702 Size: 42 127 533 Fragments arranged by size: 533 127 42 SphI G_CATG'C Cuts at: 0 233 702 Size: 233 469 StuI AGG'CCT Cuts at: 0 40 248 702 Size: 40 208 454 Fragments arranged by size: 454 208 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 678 702 Size: 678 24 XhoI C'TCGA_G Cuts at: 0 42 702 Size: 42 660 XmnI GAAnn'nnTTC Cuts at: 0 502 702 Size: 502 200 Enzymes that do cut: AfeI AlfI AlwNI AvaI BamHI BanI BanII BbeI BpuEI BsaI BsaHI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI BtgI BtsI DraI EaeI EagI EciI FseI HaeII Hin4I HincII KasI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PflMI PshAI PspXI PstI SacII SfcI SfoI SmlI SphI StuI TaqII XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaWI BsaXI BseYI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StyI SwaI TatI TstI Tth111I XbaI XcmI XmaI ZraI