Restriction map for
pFN21AE6472
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KspI MaeI
MamI MfeI MroI MseI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 628 GGTACC G^GTACC
AccII 186 CGCG CG^CG
AflIII 185 ACGCGT A^C[GA][CT]GT
AlwNI 1117 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 628 GGTACC G^GTACC
AvaI 971 CCCGAG C^[CT]CG[GA]G
AvaII 338 GGACC G^G[AT]CC
BamHI 918 GGATCC G^GATCC
BanII 65 GGGCTC G[GA]GC[CT]^C
BcnI 56 CCGGG CC^[GC]GG
BseRI 1078 GAGGAG GAGGAG(10/8)
BsoBI 971 CCCGAG C^[CT]CG[GA]G
BspMI 424 ACCTGC ACCTGC(4/8)
BsrFI 711 GCCGGC [GA]^CCGG[CT]
BsrI 1072 CCAGT ACTGG(1/-1)
BstEII 1320 GGTCACC G^GT[ACGT]ACC
BstPI 1320 GGTCACC G^GT[ACGT]ACC
BstUI 186 CGCG CG^CG
Cfr10I 711 GCCGGC [GA]^CCGG[CT]
DraII 337 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 337 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1320 GGTCACC G^GT[ACGT]ACC
HaeII 1152 GGCGCT [GA]GCGC^[CT]
HincII 181 GTCAAC GT[CT]^[GA]AC
HindII 181 GTCAAC GT[CT]^[GA]AC
KpnI 628 GGTACC GGTAC^C
MluI 185 ACGCGT A^CGCGT
MvnI 186 CGCG CG^CG
NaeI 711 GCCGGC GCC^GGC
NciI 56 CCGGG CC^[GC]GG
NcoI 848 CCATGG C^CATGG
NgoMIV 711 GCCGGC G^CCGGC
NspI 800 GCATGC [GA]CATG^[CT]
PpuMI 337 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1119 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1155 GCTCTTC GCTCTTC(1/4)
SexAI 631 ACCTGGT A^CC[AT]GGT
SmlI 42 CTTGAG C^T[CT][GA]AG
SphI 800 GCATGC GCATG^C
XcmI 1279 CCAGCCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 409 GTCTAC GT^[AC][GT]AC
868 GTCTAC
BalI 398 TGGCCA TGG^CCA
670 TGGCCA
BsgI 1015 CTGCAC GTGCAG(16/14)
197 GTGCAG
BsrBI 61 GAGCGG CCGCTC(-3/-3)
1096 GAGCGG
BsrDI 429 CATTGC GCAATG(2/0)
329 GCAATG
BstXI 1279 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1364 CCATCTTTGTGG
BstYI 918 GGATCC [GA]^GATC[CT]
1374 GGATCT
CfoI 1056 GCGC GCG^C
1153 GCGC
DsaI 848 CCATGG C^C[GA][CT]GG
959 CCGTGG
EcoT14I 511 CCTTGG C^C[AT][AT]GG
848 CCATGG
HgaI 1119 GACGC GACGC(5/10)
620 GCGTC
HhaI 1056 GCGC GCG^C
1153 GCGC
HinP1I 1056 GCGC G^CGC
1153 GCGC
HinfI 81 GAGTC G^A[ACGT]TC
1310 GAGTC
MflI 918 GGATCC [GA]^GATC[CT]
1374 GGATCT
MluNI 398 TGGCCA TGG^CCA
670 TGGCCA
MscI 398 TGGCCA TGG^CCA
670 TGGCCA
PflMI 345 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1283 CCAGCTGGTGG
PleI 81 GAGTC GAGTC(4/5)
1310 GAGTC
PvuII 842 CAGCTG CAG^CTG
1284 CAGCTG
StuI 1044 AGGCCT AGG^CCT
1197 AGGCCT
StyI 511 CCTTGG C^C[AT][AT]GG
848 CCATGG
Van91I 345 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1283 CCAGCTGGTGG
XhoII 918 GGATCC [GA]^GATC[CT]
1374 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanI BbsI BbvI BmyI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EaeI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I TspRI