(Circular) (Six-base) MAPSORT of: pFN21AE6472.seq Check: 3877 from: 1 to: 6091 pFN21AE6472 6091 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2438 2438 Size: 6091 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2415 2874 3434 1937 Size: 478 459 560 4594 Fragments arranged by size: 4594 560 478 459 Acc65I G'GTAC_C Cuts at: 2633 3412 2633 Size: 779 5312 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2215 2838 4154 5538 5806 1632 Size: 583 623 1316 1384 268 1917 Fragments arranged by size: 1917 1384 1316 623 583 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 2190 4990 5967 1203 Size: 987 2800 977 1327 Fragments arranged by size: 2800 1327 987 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 3027 1166 Size: 1861 4230 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2282 1184 Size: 1098 4993 AlwNI CAG_nnn'CTG Cuts at: 3127 3834 5406 3127 Size: 707 1572 3812 Fragments arranged by size: 3812 1572 707 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 3400 3626 3778 5887 1493 Size: 1907 226 152 2109 1697 Fragments arranged by size: 2109 1907 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2976 3416 1870 Size: 73 1033 440 4545 Fragments arranged by size: 4545 1033 440 73 BamHI G'GATC_C Cuts at: 2923 3421 3788 2923 Size: 498 367 5226 Fragments arranged by size: 5226 498 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 2426 2633 3073 3412 Size: 325 217 627 363 276 207 440 339 Cuts at: 3412 4867 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 440 363 339 325 276 217 207 BanII G_rGCy'C Cuts at: 1391 2074 3410 1391 Size: 683 1336 4072 Fragments arranged by size: 4072 1336 683 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2788 2831 3220 928 Size: 971 889 43 389 3799 Fragments arranged by size: 3799 971 889 389 43 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4282 4316 1903 Size: 34 2345 34 3678 Fragments arranged by size: 3678 2345 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4702 136 Size: 107 122 71 631 605 3030 1525 Fragments arranged by size: 3030 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 3508 3508 Size: 6091 Bme1580I G_kGCm'C Cuts at: 1790 2793 4152 5308 1790 Size: 1003 1359 1156 2573 Fragments arranged by size: 2573 1359 1156 1003 BmgBI CAC'GTC Cuts at: 1318 2779 2932 3091 1318 Size: 1461 153 159 4318 Fragments arranged by size: 4318 1461 159 153 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3822 4780 442 Size: 416 53 83 399 24 2405 958 1753 Fragments arranged by size: 2405 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4751 1239 Size: 3512 2579 Bpu10I CC'TnA_GC Cuts at: 2621 2993 2621 Size: 372 5719 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2068 4216 5081 5379 2068 Size: 2148 865 298 2780 Fragments arranged by size: 2780 2148 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4754 882 Size: 617 3255 2219 Fragments arranged by size: 3255 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3882 493 Size: 711 2678 2702 Fragments arranged by size: 2702 2678 711 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3898 4280 4915 275 Size: 53 83 186 936 2365 382 635 1451 Fragments arranged by size: 2365 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4524 4922 5196 5343 5754 4524 Size: 398 274 147 411 4861 Fragments arranged by size: 4861 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2904 2934 1178 Size: 30 165 30 1501 30 4335 Fragments arranged by size: 4335 1501 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3098 1186 Size: 1912 4179 BseYI C'CCAG_C Cuts at: 2221 5294 5790 2221 Size: 3073 496 2522 Fragments arranged by size: 3073 2522 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2223 3005 1163 Size: 573 487 782 4249 Fragments arranged by size: 4249 782 573 487 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3541 4302 5330 1540 Size: 275 186 1540 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2054 2937 3003 4152 4237 5308 2054 Size: 883 66 1149 85 1071 2837 Fragments arranged by size: 2837 1149 1071 883 85 66 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2074 2793 2937 3003 3410 4152 Size: 399 264 20 719 144 66 407 742 Cuts at: 4152 4237 5308 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 719 407 399 264 144 85 66 20 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2438 3446 844 Size: 1594 1008 3489 Fragments arranged by size: 3489 1594 1008 BsrBI CCG'CTC Cuts at: 1394 1823 2068 3103 3978 1394 Size: 429 245 1035 875 3507 Fragments arranged by size: 3507 1035 875 429 245 BsrDI GCAATG_nn' Cuts at: 65 1248 2341 2433 4586 4760 65 Size: 1183 1093 92 2153 174 1396 Fragments arranged by size: 2153 1396 1183 1093 174 92 BsrFI r'CCGG_y Cuts at: 1700 1810 2716 4735 4922 1700 Size: 110 906 2019 187 2869 Fragments arranged by size: 2869 2019 906 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6091 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 3325 1184 Size: 2141 3950 BstXI CCAn_nnnn'nTGG Cuts at: 3291 3376 3291 Size: 85 6006 BstYI r'GATC_y Cuts at: 1972 2923 3379 3421 3788 4173 4190 5642 5728 Size: 951 456 42 367 385 17 1452 86 Cuts at: 5728 5740 6086 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 951 456 385 367 346 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2853 2964 513 Size: 552 939 849 111 3640 Fragments arranged by size: 3640 939 849 552 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2245 2392 487 Size: 683 56 1019 147 4186 Fragments arranged by size: 4186 1019 683 147 56 BtsI GCAGTG_nn' Cuts at: 2235 2264 2550 2791 3607 4401 4421 2235 Size: 29 286 241 816 794 20 3905 Fragments arranged by size: 3905 816 794 286 241 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2403 2675 2961 Size: 54 1425 50 275 18 573 272 286 Cuts at: 2961 2967 3207 3349 3538 4427 6056 8 Size: 6 240 142 189 889 1629 43 Fragments arranged by size: 1629 1425 889 573 286 275 272 240 189 142 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2567 3167 3267 4021 1353 Size: 1214 600 100 754 3423 Fragments arranged by size: 3423 1214 754 600 100 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4678 5052 5198 387 Size: 1064 138 3089 374 146 1280 Fragments arranged by size: 3089 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2215 2838 4154 4751 5538 5806 1239 Size: 393 583 623 1316 597 787 268 1524 Fragments arranged by size: 1524 1316 787 623 597 583 393 268 EcoO109I rG'GnC_Cy Cuts at: 2343 2343 Size: 6091 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 3161 5238 1678 Size: 1483 2077 2531 Fragments arranged by size: 2531 2077 1483 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2970 3002 3327 3359 4732 Size: 32 135 32 1398 32 325 32 1373 Cuts at: 4732 4764 4806 4838 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1398 1373 325 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2188 3435 3681 669 Size: 988 281 250 1247 246 3079 Fragments arranged by size: 3079 1247 988 281 250 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KpnI G_GTAC'C Cuts at: 2637 3416 2637 Size: 779 5312 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 2190 5967 2190 Size: 3777 2314 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5205 5389 1305 Size: 3900 184 2007 Fragments arranged by size: 3900 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2405 2677 10 Size: 54 1425 343 573 272 3424 Fragments arranged by size: 3424 1425 573 343 272 54 MslI CAynn'nnrTG Cuts at: 518 1166 2963 3027 3374 4049 4408 4567 518 Size: 648 1797 64 347 675 359 159 2042 Fragments arranged by size: 2042 1797 675 648 359 347 159 64 MspA1I CmG'CkG Cuts at: 1606 2511 2849 3291 3507 4184 5332 5577 1606 Size: 905 338 442 216 677 1148 245 2120 Fragments arranged by size: 2120 1148 905 677 442 338 245 216 NaeI GCC'GGC Cuts at: 2718 2718 Size: 6091 NcoI C'CATG_G Cuts at: 513 1065 2004 2853 513 Size: 552 939 849 3751 Fragments arranged by size: 3751 939 849 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NgoMIV G'CCGG_C Cuts at: 2716 2716 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 2809 3449 4994 1132 Size: 97 1580 640 1545 2229 Fragments arranged by size: 2229 1580 1545 640 97 PasI CC'CwG_GG Cuts at: 1348 2586 2696 3233 3331 1348 Size: 1238 110 537 98 4108 Fragments arranged by size: 4108 1238 537 110 98 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 2356 3294 1108 Size: 1248 938 3905 Fragments arranged by size: 3905 1248 938 PfoI T'CCnGG_A Cuts at: 2723 2723 Size: 6091 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2343 2343 Size: 6091 PshAI GACnn'nnGTC Cuts at: 1613 3128 1613 Size: 1515 4576 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PstI C_TGCA'G Cuts at: 830 2085 2271 3123 3443 830 Size: 1255 186 852 320 3478 Fragments arranged by size: 3478 1255 852 320 186 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 2849 3291 2849 Size: 442 5649 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SapI GCTCTTCn'nnn_ Cuts at: 3167 3167 Size: 6091 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SexAI A'CCwGG_T Cuts at: 2636 2636 Size: 6091 SfcI C'TryA_G Cuts at: 826 1045 2032 2081 2267 3119 3439 4574 5255 Size: 219 987 49 186 852 320 1135 681 Cuts at: 5255 5446 826 Size: 191 1471 Fragments arranged by size: 1471 1135 987 852 681 320 219 191 186 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3418 1872 Size: 1546 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2047 4195 5096 5358 820 Size: 197 926 104 2148 901 262 1553 Fragments arranged by size: 2148 1553 926 901 262 197 104 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 2809 3449 1132 Size: 1677 640 3774 Fragments arranged by size: 3774 1677 640 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 3051 3204 1517 Size: 1534 153 4404 Fragments arranged by size: 4404 1534 153 StyI C'CwwG_G Cuts at: 513 1065 2004 2516 2853 3530 513 Size: 552 939 512 337 677 3074 Fragments arranged by size: 3074 939 677 552 512 337 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2189 3286 4118 4135 4288 4473 4918 Size: 1255 609 1097 832 17 153 185 445 Cuts at: 4918 325 Size: 1498 Fragments arranged by size: 1498 1255 1097 832 609 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2667 4337 6049 Size: 274 80 33 51 1443 690 1670 1712 Cuts at: 6049 96 Size: 138 Fragments arranged by size: 1712 1670 1443 690 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2904 2936 3038 3070 6051 6083 2904 Size: 32 102 32 2981 32 2912 Fragments arranged by size: 2981 2912 102 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3291 3291 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3416 1870 Size: 1546 4545 XmnI GAAnn'nnTTC Cuts at: 4220 4220 Size: 6091 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI