Restriction map for
pFN21AE6472
(Linear) (Six-base) MAPSORT of: KIEE6472.seq Check: 5783 from: 1 to: 1386
KIEE6472.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 433 1386
Size: 433 953
AccI GT'mk_AC
Cuts at: 0 410 869 1386
Size: 410 459 517
Fragments arranged by size:
517 459 410
Acc65I G'GTAC_C
Cuts at: 0 628 1386
Size: 628 758
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 833 1386
Size: 210 623 553
Fragments arranged by size:
623 553 210
AflIII A'CryG_T
Cuts at: 0 185 1386
Size: 185 1201
AleI CACnn'nnGTG
Cuts at: 0 1022 1386
Size: 1022 364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 277 1386
Size: 277 1109
AlwNI CAG_nnn'CTG
Cuts at: 0 1122 1386
Size: 1122 264
AvaI C'yCGr_G
Cuts at: 0 971 1386
Size: 971 415
BamHI G'GATC_C
Cuts at: 0 918 1386
Size: 918 468
BanI G'GyrC_C
Cuts at: 0 145 421 628 1068 1386
Size: 145 276 207 440 318
Fragments arranged by size:
440 318 276 207 145
BanII G_rGCy'C
Cuts at: 0 69 1386
Size: 69 1317
BbsI GAAGACnn'nnnn_
Cuts at: 0 783 826 1215 1386
Size: 783 43 389 171
Fragments arranged by size:
783 389 171 43
Bme1580I G_kGCm'C
Cuts at: 0 788 1386
Size: 788 598
BmgBI CAC'GTC
Cuts at: 0 774 927 1086 1386
Size: 774 153 159 300
Fragments arranged by size:
774 300 159 153
Bpu10I CC'TnA_GC
Cuts at: 0 616 988 1386
Size: 616 372 398
Fragments arranged by size:
616 398 372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 1386
Size: 63 1323
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 899 929 1386
Size: 899 30 457
Fragments arranged by size:
899 457 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1093 1386
Size: 1093 293
BseYI C'CCAG_C
Cuts at: 0 216 1386
Size: 216 1170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 1000 1386
Size: 218 782 386
Fragments arranged by size:
782 386 218
BsiHKAI G_wGCw'C
Cuts at: 0 49 932 998 1386
Size: 49 883 66 388
Fragments arranged by size:
883 388 66 49
Bsp1286I G_dGCh'C
Cuts at: 0 49 69 788 932 998 1386
Size: 49 20 719 144 66 388
Fragments arranged by size:
719 388 144 66 49 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 433 1386
Size: 433 953
BsrBI CCG'CTC
Cuts at: 0 63 1098 1386
Size: 63 1035 288
Fragments arranged by size:
1035 288 63
BsrDI GCAATG_nn'
Cuts at: 0 336 428 1386
Size: 336 92 958
Fragments arranged by size:
958 336 92
BsrFI r'CCGG_y
Cuts at: 0 711 1386
Size: 711 675
BstEII G'GTnAC_C
Cuts at: 0 1320 1386
Size: 1320 66
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1286 1371 1386
Size: 1286 85 15
Fragments arranged by size:
1286 85 15
BstYI r'GATC_y
Cuts at: 0 918 1374 1386
Size: 918 456 12
Fragments arranged by size:
918 456 12
BtgI C'CryG_G
Cuts at: 0 848 959 1386
Size: 848 111 427
Fragments arranged by size:
848 427 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 240 387 1386
Size: 240 147 999
Fragments arranged by size:
999 240 147
BtsI GCAGTG_nn'
Cuts at: 0 230 259 545 786 1386
Size: 230 29 286 241 600
Fragments arranged by size:
600 286 241 230 29
EaeI y'GGCC_r
Cuts at: 0 398 670 956 962 1202 1344 1386
Size: 398 272 286 6 240 142 42
Fragments arranged by size:
398 286 272 240 142 42 6
EarI CTCTTCn'nnn_
Cuts at: 0 562 1162 1262 1386
Size: 562 600 100 124
Fragments arranged by size:
600 562 124 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 833 1386
Size: 210 623 553
Fragments arranged by size:
623 553 210
EcoO109I rG'GnC_Cy
Cuts at: 0 338 1386
Size: 338 1048
HaeII r_GCGC'y
Cuts at: 0 1156 1386
Size: 1156 230
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 965 997 1322 1354 1386
Size: 965 32 325 32 32
Fragments arranged by size:
965 325 32 32 32
HincII GTy'rAC
Cuts at: 0 183 1386
Size: 183 1203
KpnI G_GTAC'C
Cuts at: 0 632 1386
Size: 632 754
MluI A'CGCG_T
Cuts at: 0 185 1386
Size: 185 1201
MscI TGG'CCA
Cuts at: 0 400 672 1386
Size: 400 272 714
Fragments arranged by size:
714 400 272
MslI CAynn'nnrTG
Cuts at: 0 958 1022 1369 1386
Size: 958 64 347 17
Fragments arranged by size:
958 347 64 17
MspA1I CmG'CkG
Cuts at: 0 506 844 1286 1386
Size: 506 338 442 100
Fragments arranged by size:
506 442 338 100
NaeI GCC'GGC
Cuts at: 0 713 1386
Size: 713 673
NcoI C'CATG_G
Cuts at: 0 848 1386
Size: 848 538
NgoMIV G'CCGG_C
Cuts at: 0 711 1386
Size: 711 675
NspI r_CATG'y
Cuts at: 0 804 1386
Size: 804 582
PasI CC'CwG_GG
Cuts at: 0 581 691 1228 1326 1386
Size: 581 110 537 98 60
Fragments arranged by size:
581 537 110 98 60
PflMI CCAn_nnn'nTGG
Cuts at: 0 351 1289 1386
Size: 351 938 97
Fragments arranged by size:
938 351 97
PfoI T'CCnGG_A
Cuts at: 0 718 1386
Size: 718 668
PpuMI rG'GwC_Cy
Cuts at: 0 338 1386
Size: 338 1048
PshAI GACnn'nnGTC
Cuts at: 0 1123 1386
Size: 1123 263
PstI C_TGCA'G
Cuts at: 0 80 266 1118 1386
Size: 80 186 852 268
Fragments arranged by size:
852 268 186 80
PvuII CAG'CTG
Cuts at: 0 844 1286 1386
Size: 844 442 100
Fragments arranged by size:
844 442 100
SapI GCTCTTCn'nnn_
Cuts at: 0 1162 1386
Size: 1162 224
SexAI A'CCwGG_T
Cuts at: 0 631 1386
Size: 631 755
SfcI C'TryA_G
Cuts at: 0 27 76 262 1114 1386
Size: 27 49 186 852 272
Fragments arranged by size:
852 272 186 49 27
SmlI C'TyrA_G
Cuts at: 0 42 1386
Size: 42 1344
SphI G_CATG'C
Cuts at: 0 804 1386
Size: 804 582
StuI AGG'CCT
Cuts at: 0 1046 1199 1386
Size: 1046 153 187
Fragments arranged by size:
1046 187 153
StyI C'CwwG_G
Cuts at: 0 511 848 1386
Size: 511 337 538
Fragments arranged by size:
538 511 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 1281 1386
Size: 184 1097 105
Fragments arranged by size:
1097 184 105
TatI w'GTAC_w
Cuts at: 0 662 1386
Size: 662 724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 899 931 1033 1065 1386
Size: 899 32 102 32 321
Fragments arranged by size:
899 321 102 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1286 1386
Size: 1286 100
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlfI AlwNI
AvaI BamHI BanI BanII BbsI Bme1580I BmgBI Bpu10I
BpuEI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BstEII BstXI BstYI BtgI BtgZI
BtsI EaeI EarI Eco57MI EcoO109I HaeII Hin4I HincII
KpnI MluI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PasI PflMI PfoI PpuMI PshAI PstI PvuII
SapI SexAI SfcI SmlI SphI StuI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AloI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmrI BmtI BplI BpmI BsaI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
MfeI MmeI NarI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI