(Circular) (Six-base) MAPSORT of: pFN21AE1149.seq Check: 3278 from: 1 to: 6052 pFN21AE1149 6052 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2246 2246 Size: 6052 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3862 278 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3395 1937 Size: 1458 4594 Acc65I G'GTAC_C Cuts at: 3373 3373 Size: 6052 AclI AA'CG_TT Cuts at: 1563 4179 4552 1563 Size: 2616 373 3063 Fragments arranged by size: 3063 2616 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2524 2937 3042 4115 5499 5767 1632 Size: 892 413 105 1073 1384 268 1917 Fragments arranged by size: 1917 1384 1073 892 413 268 105 AfeI AGC'GCT Cuts at: 2945 2945 Size: 6052 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5855 AflIII A'CryG_T Cuts at: 1203 4951 5928 1203 Size: 3748 977 1327 Fragments arranged by size: 3748 1327 977 AgeI A'CCGG_T Cuts at: 4883 4883 Size: 6052 AhdI GACnn_n'nnGTC Cuts at: 4781 4781 Size: 6052 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6052 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3228 1184 Size: 2044 4008 AlwNI CAG_nnn'CTG Cuts at: 3795 5367 3795 Size: 1572 4480 ApaI G_GGCC'C Cuts at: 2043 2043 Size: 6052 ApaLI G'TGCA_C Cuts at: 4109 5265 4109 Size: 1156 4896 ApoI r'AATT_y Cuts at: 1493 3361 3587 3739 5848 1493 Size: 1868 226 152 2109 1697 Fragments arranged by size: 2109 1868 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4606 160 Size: 872 3574 1606 Fragments arranged by size: 3574 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 AvaI C'yCGr_G Cuts at: 1870 1943 2169 2375 3377 1870 Size: 73 226 206 1002 4545 Fragments arranged by size: 4545 1002 226 206 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2433 2466 2433 Size: 33 6019 BamHI G'GATC_C Cuts at: 2062 3382 3749 2062 Size: 1320 367 4365 Fragments arranged by size: 4365 1320 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2088 2206 2299 2427 2662 Size: 325 217 627 301 118 93 128 235 Cuts at: 2662 2928 3373 4828 618 Size: 266 445 1455 1842 Fragments arranged by size: 1842 1455 627 445 325 301 266 235 217 128 118 93 BanII G_rGCy'C Cuts at: 1391 2043 2646 3371 1391 Size: 652 603 725 4072 Fragments arranged by size: 4072 725 652 603 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2723 928 Size: 971 824 4257 Fragments arranged by size: 4257 971 824 BbvCI CC'TCA_GC Cuts at: 3066 3066 Size: 6052 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4243 4277 1903 Size: 34 2306 34 3678 Fragments arranged by size: 3678 2306 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3944 5154 3944 Size: 1210 4842 BclI T'GATC_A Cuts at: 1548 1548 Size: 6052 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2139 2292 4663 Size: 107 122 71 631 605 467 153 2371 Cuts at: 4663 136 Size: 1525 Fragments arranged by size: 2371 1525 631 605 467 153 122 107 71 BglII A'GATC_T Cuts at: 6047 6047 Size: 6052 BlpI GC'TnA_GC Cuts at: 3469 3469 Size: 6052 Bme1580I G_kGCm'C Cuts at: 1790 2043 2144 2444 4113 5269 1790 Size: 253 101 300 1669 1156 2573 Fragments arranged by size: 2573 1669 1156 300 253 101 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6052 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3783 4741 442 Size: 416 53 83 399 24 2366 958 1753 Fragments arranged by size: 2366 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6052 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2665 2665 Size: 6052 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2279 2371 2420 2639 2987 3298 4712 Size: 887 153 92 49 219 348 311 1414 Cuts at: 4712 1239 Size: 2579 Fragments arranged by size: 2579 1414 887 348 311 219 153 92 49 Bpu10I CC'TnA_GC Cuts at: 2081 3066 2081 Size: 985 5067 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4177 5042 5340 4177 Size: 865 298 4889 Fragments arranged by size: 4889 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4715 882 Size: 617 3216 2219 Fragments arranged by size: 3216 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3843 493 Size: 711 2639 2702 Fragments arranged by size: 2702 2639 711 BsaBI GATnn'nnATC Cuts at: 6046 6046 Size: 6052 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3859 4241 4876 275 Size: 53 83 186 936 2326 382 635 1451 Fragments arranged by size: 2326 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4485 4883 5157 5304 5715 4485 Size: 398 274 147 411 4822 Fragments arranged by size: 4822 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2225 2255 2411 2441 2979 Size: 30 165 30 822 30 156 30 538 Cuts at: 2979 3009 1178 Size: 30 4221 Fragments arranged by size: 4221 822 538 165 156 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2429 2432 3023 3041 3083 1186 Size: 1243 3 591 18 42 4155 Fragments arranged by size: 4155 1243 591 42 18 3 BseYI C'CCAG_C Cuts at: 2295 2380 2946 5255 5751 2295 Size: 85 566 2309 496 2596 Fragments arranged by size: 2596 2309 566 496 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2201 1163 Size: 573 465 5014 Fragments arranged by size: 5014 573 465 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3502 4263 5291 1540 Size: 275 186 1501 761 1028 2301 Fragments arranged by size: 2301 1501 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2397 4113 4198 5269 2397 Size: 1716 85 1071 3180 Fragments arranged by size: 3180 1716 1071 85 BsmI GAATG_Cn' Cuts at: 3563 3656 3563 Size: 93 5959 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2144 2397 2444 2646 3371 4113 Size: 399 253 101 253 47 202 725 742 Cuts at: 4113 4198 5269 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 725 399 253 253 202 101 85 47 BspEI T'CCGG_A Cuts at: 5715 5715 Size: 6052 BspHI T'CATG_A Cuts at: 3941 5671 3941 Size: 1730 4322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2246 3407 844 Size: 1402 1161 3489 Fragments arranged by size: 3489 1402 1161 BsrBI CCG'CTC Cuts at: 1394 1823 3939 1394 Size: 429 2116 3507 Fragments arranged by size: 3507 2116 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4547 4721 65 Size: 1183 3299 174 1396 Fragments arranged by size: 3299 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2091 2133 2150 2424 4696 4883 1700 Size: 110 281 42 17 274 2272 187 2869 Fragments arranged by size: 2869 2272 281 274 187 110 42 17 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6052 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6052 BssSI C'ACGA_G Cuts at: 2324 4112 5124 2324 Size: 1788 1012 3252 Fragments arranged by size: 3252 1788 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6052 BstBI TT'CG_AA Cuts at: 4856 4856 Size: 6052 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6052 BstYI r'GATC_y Cuts at: 1972 2062 3382 3749 4134 4151 5603 5689 5701 Size: 90 1320 367 385 17 1452 86 12 Cuts at: 5701 6047 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1320 385 367 346 90 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2454 2546 2454 Size: 92 5960 BtgI C'CryG_G Cuts at: 513 1065 2004 2027 2043 2210 2460 513 Size: 552 939 23 16 167 250 4105 Fragments arranged by size: 4105 939 552 250 167 23 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5313 Fragments arranged by size: 5313 683 56 BtsI GCAGTG_nn' Cuts at: 2303 3568 4362 4382 2303 Size: 1265 794 20 3973 Fragments arranged by size: 3973 1265 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6052 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6017 DraI TTT'AAA Cuts at: 3356 3356 Size: 6052 DraIII CAC_nnn'GTG Cuts at: 2935 2935 Size: 6052 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5059 809 Size: 507 3743 1802 Fragments arranged by size: 3743 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2408 2475 Size: 54 1425 50 275 18 318 260 67 Cuts at: 2475 3499 4388 6017 8 Size: 1024 889 1629 43 Fragments arranged by size: 1629 1425 1024 889 318 275 260 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3499 1537 Size: 275 1687 4090 Fragments arranged by size: 4090 1687 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2543 2622 2976 3018 3114 3982 1353 Size: 1190 79 354 42 96 868 3423 Fragments arranged by size: 3423 1190 868 354 96 79 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4639 5013 5159 387 Size: 1064 138 3050 374 146 1280 Fragments arranged by size: 3050 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2279 2371 2420 2524 2639 2937 Size: 393 494 153 92 49 104 115 298 Cuts at: 2937 2987 3042 3298 4115 4712 5499 5767 1239 Size: 50 55 256 817 597 787 268 1524 Fragments arranged by size: 1524 817 787 597 494 393 298 268 256 153 115 104 92 55 50 49 EcoNI CCTnn'n_nnAGG Cuts at: 2435 2435 Size: 6052 EcoO109I rG'GnC_Cy Cuts at: 2039 2335 2355 2039 Size: 296 20 5736 Fragments arranged by size: 5736 296 20 EcoRI G'AATT_C Cuts at: 3361 3739 3361 Size: 378 5674 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3340 3340 Size: 6052 FspI TGC'GCA Cuts at: 4558 4558 Size: 6052 HaeII r_GCGC'y Cuts at: 1678 2318 2425 2947 5199 1678 Size: 640 107 522 2252 2531 Fragments arranged by size: 2531 2252 640 522 107 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2483 2515 3313 3345 4693 Size: 32 135 32 911 32 798 32 1348 Cuts at: 4693 4725 4767 4799 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1348 911 798 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3396 3642 669 Size: 988 281 1458 246 3079 Fragments arranged by size: 3079 1458 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6052 HpaI GTT'AAC Cuts at: 3642 3642 Size: 6052 KpnI G_GTAC'C Cuts at: 3377 3377 Size: 6052 MfeI C'AATT_G Cuts at: 3651 3651 Size: 6052 MluI A'CGCG_T Cuts at: 5928 5928 Size: 6052 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3318 5166 5350 1305 Size: 2013 1848 184 2007 Fragments arranged by size: 2013 2007 1848 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2410 2477 10 Size: 54 1425 343 578 67 3585 Fragments arranged by size: 3585 1425 578 343 67 54 MslI CAynn'nnrTG Cuts at: 518 1166 2873 4010 4369 4528 518 Size: 648 1707 1137 359 159 2042 Fragments arranged by size: 2042 1707 1137 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2212 2578 2864 3175 3276 3468 4145 5293 Size: 606 366 286 311 101 192 677 1148 Cuts at: 5293 5538 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 606 366 311 286 245 192 101 NaeI GCC'GGC Cuts at: 2093 2135 2152 2093 Size: 42 17 5993 Fragments arranged by size: 5993 42 17 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4561 Fragments arranged by size: 4561 939 552 NdeI CA'TA_TG Cuts at: 387 2817 387 Size: 2430 3622 NgoMIV G'CCGG_C Cuts at: 2091 2133 2150 2091 Size: 42 17 5993 Fragments arranged by size: 5993 42 17 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6052 NotI GC'GGCC_GC Cuts at: 3499 3499 Size: 6052 NspI r_CATG'y Cuts at: 1132 1229 3061 3410 4955 1132 Size: 97 1832 349 1545 2229 Fragments arranged by size: 2229 1832 1545 349 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6052 PciI A'CATG_T Cuts at: 4951 4951 Size: 6052 PflMI CCAn_nnn'nTGG Cuts at: 1108 2578 2581 1108 Size: 1470 3 4579 Fragments arranged by size: 4579 1470 3 PmeI GTTT'AAAC Cuts at: 3356 3356 Size: 6052 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2183 2215 4121 4153 5634 5666 2183 Size: 32 1906 32 1481 32 2569 Fragments arranged by size: 2569 1906 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6052 PsiI TTA'TAA Cuts at: 3622 3622 Size: 6052 PspOMI G'GGCC_C Cuts at: 2039 2039 Size: 6052 PstI C_TGCA'G Cuts at: 830 2283 2334 2674 3404 830 Size: 1453 51 340 730 3478 Fragments arranged by size: 3478 1453 730 340 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6052 PvuII CAG'CTG Cuts at: 2578 2864 3175 3276 2578 Size: 286 311 101 5354 Fragments arranged by size: 5354 311 286 101 SacII CC_GC'GG Cuts at: 2213 2213 Size: 6052 SalI G'TCGA_C Cuts at: 1936 3394 1936 Size: 1458 4594 SbfI CC_TGCA'GG Cuts at: 3404 3404 Size: 6052 ScaI AGT'ACT Cuts at: 4300 6012 4300 Size: 1712 4340 SfcI C'TryA_G Cuts at: 826 1045 2279 2330 2599 2670 3400 4535 5216 Size: 219 1234 51 269 71 730 1135 681 Cuts at: 5216 5407 826 Size: 191 1471 Fragments arranged by size: 1471 1234 1135 730 681 269 219 191 71 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 SgrAI Cr'CCGG_yG Cuts at: 1700 2424 1700 Size: 724 5328 SmaI CCC'GGG Cuts at: 1872 2171 3379 1872 Size: 299 1208 4545 Fragments arranged by size: 4545 1208 299 SmlI C'TyrA_G Cuts at: 820 1017 1943 4156 5057 5319 820 Size: 197 926 2213 901 262 1553 Fragments arranged by size: 2213 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3843 493 Size: 3350 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6052 SphI G_CATG'C Cuts at: 1132 3061 3410 1132 Size: 1929 349 3774 Fragments arranged by size: 3774 1929 349 SspI AAT'ATT Cuts at: 5 52 3145 3976 5 Size: 47 3093 831 2081 Fragments arranged by size: 3093 2081 831 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6052 StyI C'CwwG_G Cuts at: 513 1065 2004 2118 3235 3491 513 Size: 552 939 114 1117 256 3074 Fragments arranged by size: 3074 1117 939 552 256 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2287 4079 4096 4249 4434 4879 325 Size: 1255 707 1792 17 153 185 445 1498 Fragments arranged by size: 1792 1498 1255 707 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4298 6010 96 Size: 274 80 33 51 1443 2321 1712 138 Fragments arranged by size: 2321 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2225 2257 2411 2443 2979 3011 3130 3162 6012 Size: 32 154 32 536 32 119 32 2850 Cuts at: 6012 6044 2225 Size: 32 2233 Fragments arranged by size: 2850 2233 536 154 119 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6052 XbaI T'CTAG_A Cuts at: 2710 3388 2710 Size: 678 5374 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6052 XmaI C'CCGG_G Cuts at: 1870 2169 3377 1870 Size: 299 1208 4545 Fragments arranged by size: 4545 1208 299 XmnI GAAnn'nnTTC Cuts at: 4181 4181 Size: 6052 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3860 276 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI