Restriction map for
pFN21AE5249
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AvrII BamHI
BanII BbrPI BbvCI BcgI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HgaI HindIII HinfI HpaI
KpnI KspI MaeI MaeII MamI
MfeI MflI MluI MroI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI TfiI Tsp509I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 52 GGCGCC G[GA]^CG[CT]C
AhdI 33 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 390 CCTGAGG CC^T[ACGT]AGG
AspEI 33 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 109 CTCGGG C^[CT]CG[GA]G
AviII 45 TGCGCA TGC^GCA
BalI 298 TGGCCA TGG^CCA
BbeI 52 GGCGCC GGCGC^C
BbsI 220 GTCTTC GAAGAC(2/6)
BciVI 186 GTATCC GTATCC(6/5)
BmyI 396 GGGCAC G[AGT]GC[ACT]^C
BpuAI 220 GTCTTC GAAGAC(2/6)
BsaHI 52 GGCGCC G[GA]^CG[CT]C
BseRI 497 GAGGAG GAGGAG(10/8)
BsgI 271 CTGCAC GTGCAG(16/14)
BsoBI 109 CTCGGG C^[CT]CG[GA]G
Bsp1286I 396 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 284 TGTACA T^GTACA
BsrDI 509 GCAATG GCAATG(2/0)
BsrGI 284 TGTACA T^GTACA
BssHII 417 GCGCGC G^CGCGC
BstAPI 129 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 386 CATCC GGATG(2/0)
BstXI 521 CCAGTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 390 CCTGAGG CC^T[ACGT]AGG
DdeI 391 CTGAG C^T[ACGT]AG
Eam1105I 33 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 247 CTCTTC CTCTTC(1/4)
Eco81I 390 CCTGAGG CC^T[ACGT]AGG
FauI 104 CCCGC CCCGC(4/6)
FokI 386 CATCC GGATG(9/13)
FspI 45 TGCGCA TGC^GCA
Hin1I 52 GGCGCC G[GA]^CG[CT]C
HincII 376 GTTGAC GT[CT]^[GA]AC
HindII 376 GTTGAC GT[CT]^[GA]AC
KasI 52 GGCGCC G^GCGCC
Ksp632I 247 CTCTTC CTCTTC(1/4)
MaeIII 235 GTGAC ^GT[ACGT]AC
MluNI 298 TGGCCA TGG^CCA
MscI 298 TGGCCA TGG^CCA
NarI 52 GGCGCC GG^CGCC
PshAI 237 GACAATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 127 CTGCAG CTGCA^G
SfcI 127 CTGCAG C^T[GA][CT]AG
SfoI 52 GGCGCC GGC^GCC
SspBI 284 TGTACA T^GTACA
StuI 488 AGGCCT AGG^CCT
TaqI 16 TCGA T^CGA
Tsp45I 235 GTGAC ^GT[GC]AC
TspRI 302 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 16 TCGA T^CGA
XcmI 518 CCACCAGTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 177 GTAC GT^AC
285 GTAC
AvaII 32 GGACC G^G[AT]CC
84 GGACC
BanI 52 GGCGCC G^G[CT][GA]CC
397 GGCACC
BcnI 81 CCCGG CC^[GC]GG
401 CCCGG
BsrI 303 ACTGG ACTGG(1/-1)
521 CCAGT
Cfr13I 32 GGACC G^G[ACGT]CC
84 GGACC
DpnII 315 GATC ^GATC
453 GATC
MboI 315 GATC ^GATC
453 GATC
MseI 310 TTAA T^TAA
320 TTAA
MspA1I 130 CAGCTG C[AC]G^C[GT]G
268 CAGCTG
NciI 81 CCCGG CC^[GC]GG
401 CCCGG
NdeII 315 GATC ^GATC
453 GATC
PvuII 130 CAGCTG CAG^CTG
268 CAGCTG
RsaI 177 GTAC GT^AC
285 GTAC
Sau3AI 315 GATC ^GATC
453 GATC
Sau96I 32 GGACC G^G[ACGT]CC
84 GGACC
Tru9I 310 TTAA T^TAA
320 TTAA
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AccII AciI AluI BbvI BsaJI
BsiYI BslI BssKI BstNI BstUI
Cac8I CfoI EaeI EcoRII Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MboII MnlI
MspI MvaI MvnI MwoI NlaIII
NlaIV ScrFI TseI