(Circular) (Six-base) MAPSORT of: pFN21AE5249.seq Check: 5530 from: 1 to: 5251 pFN21AE5249 5251 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3061 278 Size: 53 83 186 936 1525 2468 Fragments arranged by size: 2468 1525 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2594 1937 Size: 657 4594 Acc65I G'GTAC_C Cuts at: 2572 2572 Size: 5251 AclI AA'CG_TT Cuts at: 1563 3378 3751 1563 Size: 1815 373 3063 Fragments arranged by size: 3063 1815 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2467 3314 4698 4966 1632 Size: 835 847 1384 268 1917 Fragments arranged by size: 1917 1384 847 835 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5054 AflIII A'CryG_T Cuts at: 1203 4150 5127 1203 Size: 2947 977 1327 Fragments arranged by size: 2947 1327 977 AgeI A'CCGG_T Cuts at: 4082 4082 Size: 5251 AhdI GACnn_n'nnGTC Cuts at: 2043 3980 2043 Size: 1937 3314 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5251 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5251 AlwNI CAG_nnn'CTG Cuts at: 2994 4566 2994 Size: 1572 3679 ApaLI G'TGCA_C Cuts at: 3308 4464 3308 Size: 1156 4095 ApoI r'AATT_y Cuts at: 1493 2560 2786 2938 5047 1493 Size: 1067 226 152 2109 1697 Fragments arranged by size: 2109 1697 1067 226 152 AseI AT'TA_AT Cuts at: 160 1032 3805 160 Size: 872 2773 1606 Fragments arranged by size: 2773 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5251 AvaI C'yCGr_G Cuts at: 1870 1943 2114 2576 1870 Size: 73 171 462 4545 Fragments arranged by size: 4545 462 171 73 BamHI G'GATC_C Cuts at: 2581 2948 2581 Size: 367 4884 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2057 2402 2572 4027 618 Size: 325 217 627 270 345 170 1455 1842 Fragments arranged by size: 1842 1455 627 345 325 270 217 170 BanII G_rGCy'C Cuts at: 1391 2570 1391 Size: 1179 4072 BbeI G_GCGC'C Cuts at: 2061 2061 Size: 5251 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2218 928 Size: 971 319 3961 Fragments arranged by size: 3961 971 319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3442 3476 1903 Size: 34 1505 34 3678 Fragments arranged by size: 3678 1505 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2202 3143 4353 2202 Size: 941 1210 3100 Fragments arranged by size: 3100 1210 941 BclI T'GATC_A Cuts at: 1548 1548 Size: 5251 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3862 136 Size: 107 122 71 631 605 2190 1525 Fragments arranged by size: 2190 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5246 5246 Size: 5251 BlpI GC'TnA_GC Cuts at: 2668 2668 Size: 5251 Bme1580I G_kGCm'C Cuts at: 1790 2405 3312 4468 1790 Size: 615 907 1156 2573 Fragments arranged by size: 2573 1156 907 615 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5251 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2982 3940 442 Size: 416 53 83 399 24 1565 958 1753 Fragments arranged by size: 1753 1565 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5251 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3911 1239 Size: 2672 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3376 4241 4539 3376 Size: 865 298 4088 Fragments arranged by size: 4088 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3914 882 Size: 617 2415 2219 Fragments arranged by size: 2415 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3042 493 Size: 711 1838 2702 Fragments arranged by size: 2702 1838 711 BsaBI GATnn'nnATC Cuts at: 5245 5245 Size: 5251 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2058 3058 3440 4075 Size: 53 83 186 936 525 1000 382 635 Cuts at: 4075 275 Size: 1451 Fragments arranged by size: 1451 1000 936 635 525 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3684 4082 4356 4503 4914 3684 Size: 398 274 147 411 4021 Fragments arranged by size: 4021 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5026 Fragments arranged by size: 5026 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2517 1186 Size: 1331 3920 BseYI C'CCAG_C Cuts at: 2143 2205 4454 4950 2143 Size: 62 2249 496 2444 Fragments arranged by size: 2444 2249 496 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2261 1163 Size: 573 525 4153 Fragments arranged by size: 4153 573 525 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2701 3462 4490 1540 Size: 275 186 700 761 1028 2301 Fragments arranged by size: 2301 1028 761 700 275 186 BsiHKAI G_wGCw'C Cuts at: 3312 3397 4468 3312 Size: 85 1071 4095 Fragments arranged by size: 4095 1071 85 BsmI GAATG_Cn' Cuts at: 2762 2855 2762 Size: 93 5158 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2405 2570 3312 3397 4468 1391 Size: 399 615 165 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 615 399 165 85 BspEI T'CCGG_A Cuts at: 4914 4914 Size: 5251 BspHI T'CATG_A Cuts at: 3140 4870 3140 Size: 1730 3521 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2606 844 Size: 1762 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3138 1394 Size: 429 1315 3507 Fragments arranged by size: 3507 1315 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2521 3746 3920 65 Size: 1183 1273 1225 174 1396 Fragments arranged by size: 1396 1273 1225 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3895 4082 1700 Size: 110 2085 187 2869 Fragments arranged by size: 2869 2085 187 110 BsrGI T'GTAC_A Cuts at: 96 2289 96 Size: 2193 3058 BssHII G'CGCG_C Cuts at: 1925 2422 1925 Size: 497 4754 BssSI C'ACGA_G Cuts at: 3311 4323 3311 Size: 1012 4239 BstAPI GCAn_nnn'nTGC Cuts at: 794 2140 794 Size: 1346 3905 BstBI TT'CG_AA Cuts at: 4055 4055 Size: 5251 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5251 BstXI CCAn_nnnn'nTGG Cuts at: 2533 2533 Size: 5251 BstYI r'GATC_y Cuts at: 1972 2581 2948 3333 3350 4802 4888 4900 5246 Size: 609 367 385 17 1452 86 12 346 Cuts at: 5246 1972 Size: 1977 Fragments arranged by size: 1977 1452 609 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2396 2396 Size: 5251 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3760 Fragments arranged by size: 3760 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2347 487 Size: 683 56 1121 3391 Fragments arranged by size: 3391 1121 683 56 BtsI GCAGTG_nn' Cuts at: 2767 3561 3581 2767 Size: 794 20 4437 Fragments arranged by size: 4437 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5251 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5216 DraI TTT'AAA Cuts at: 2555 2555 Size: 5251 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4258 809 Size: 507 2942 1802 Fragments arranged by size: 2942 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2303 2520 Size: 54 1425 50 275 18 177 296 217 Cuts at: 2520 2698 3587 5216 8 Size: 178 889 1629 43 Fragments arranged by size: 1629 1425 889 296 275 217 178 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2698 1537 Size: 275 886 4090 Fragments arranged by size: 4090 886 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2258 3181 1353 Size: 905 923 3423 Fragments arranged by size: 3423 923 905 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3838 4212 4358 387 Size: 1064 138 2249 374 146 1280 Fragments arranged by size: 2249 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2467 3314 3911 4698 4966 1239 Size: 393 835 847 597 787 268 1524 Fragments arranged by size: 1524 847 835 787 597 393 268 EcoRI G'AATT_C Cuts at: 2560 2938 2560 Size: 378 4873 FspI TGC'GCA Cuts at: 2052 3757 2052 Size: 1705 3546 HaeII r_GCGC'y Cuts at: 1678 2061 2081 2123 2165 2337 4398 1678 Size: 383 20 42 42 172 2061 2531 Fragments arranged by size: 2531 2061 383 172 42 42 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2029 2035 2061 2375 Size: 32 135 32 431 26 6 26 314 Cuts at: 2375 2407 3892 3924 3966 3998 1373 Size: 32 1485 32 42 32 2626 Fragments arranged by size: 2626 1485 431 314 135 42 32 32 32 32 32 26 26 6 HincII GTy'rAC Cuts at: 669 1657 1938 2383 2595 2841 669 Size: 988 281 445 212 246 3079 Fragments arranged by size: 3079 988 445 281 246 212 HindIII A'AGCT_T Cuts at: 748 748 Size: 5251 HpaI GTT'AAC Cuts at: 2841 2841 Size: 5251 KasI G'GCGC_C Cuts at: 2057 2057 Size: 5251 KpnI G_GTAC'C Cuts at: 2576 2576 Size: 5251 MfeI C'AATT_G Cuts at: 2850 2850 Size: 5251 MluI A'CGCG_T Cuts at: 5127 5127 Size: 5251 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4365 4549 1305 Size: 3060 184 2007 Fragments arranged by size: 3060 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2305 10 Size: 54 1425 343 473 2956 Fragments arranged by size: 2956 1425 473 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3209 3568 3727 518 Size: 648 2043 359 159 2042 Fragments arranged by size: 2043 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2275 2667 3344 4492 4737 1606 Size: 531 138 392 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 531 392 245 138 NarI GG'CG_CC Cuts at: 2058 2058 Size: 5251 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3760 Fragments arranged by size: 3760 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5251 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5251 NotI GC'GGCC_GC Cuts at: 2698 2698 Size: 5251 NspI r_CATG'y Cuts at: 1132 1229 2609 4154 1132 Size: 97 1380 1545 2229 Fragments arranged by size: 2229 1545 1380 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5251 PciI A'CATG_T Cuts at: 4150 4150 Size: 5251 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5251 PmeI GTTT'AAAC Cuts at: 2555 2555 Size: 5251 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3320 3352 4833 4865 3320 Size: 32 1481 32 3706 Fragments arranged by size: 3706 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2246 1613 Size: 633 4618 PsiI TTA'TAA Cuts at: 2415 2821 2415 Size: 406 4845 PstI C_TGCA'G Cuts at: 830 2136 2603 830 Size: 1306 467 3478 Fragments arranged by size: 3478 1306 467 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5251 PvuII CAG'CTG Cuts at: 2137 2275 2137 Size: 138 5113 SalI G'TCGA_C Cuts at: 1936 2593 1936 Size: 657 4594 SbfI CC_TGCA'GG Cuts at: 2603 2603 Size: 5251 ScaI AGT'ACT Cuts at: 3499 5211 3499 Size: 1712 3539 SfcI C'TryA_G Cuts at: 826 1045 2132 2599 3734 4415 4606 826 Size: 219 1087 467 1135 681 191 1471 Fragments arranged by size: 1471 1135 1087 681 467 219 191 SfoI GGC'GCC Cuts at: 2059 2059 Size: 5251 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5251 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5251 SmaI CCC'GGG Cuts at: 1872 2578 1872 Size: 706 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3355 4256 4518 820 Size: 197 926 1412 901 262 1553 Fragments arranged by size: 1553 1412 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3042 493 Size: 2549 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5251 SphI G_CATG'C Cuts at: 1132 2609 1132 Size: 1477 3774 SspI AAT'ATT Cuts at: 5 52 3175 5 Size: 47 3123 2081 Fragments arranged by size: 3123 2081 47 StuI AGG'CCT Cuts at: 1517 2495 1517 Size: 978 4273 StyI C'CwwG_G Cuts at: 513 1065 2004 2690 513 Size: 552 939 686 3074 Fragments arranged by size: 3074 939 686 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2197 3278 3295 3448 3633 4078 325 Size: 1255 617 1081 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1081 617 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2289 3497 5209 Size: 274 80 33 51 1443 312 1208 1712 Cuts at: 5209 96 Size: 138 Fragments arranged by size: 1712 1443 1208 312 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5211 5243 5211 Size: 32 5219 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5251 XbaI T'CTAG_A Cuts at: 2587 2587 Size: 5251 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2530 2530 Size: 5251 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5251 XmaI C'CCGG_G Cuts at: 1870 2576 1870 Size: 706 4545 XmnI GAAnn'nnTTC Cuts at: 3380 3380 Size: 5251 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3059 276 Size: 53 83 186 936 1525 2468 Fragments arranged by size: 2468 1525 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI