################################## # NO CUT ENZYMES ( 193 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DpnII DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcyI 4 GACGCC G[GA]^CG[CT]C AfaI 141 GTAC GT^AC AflII 414 CTTAAG C^TTAAG AlwNI 238 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG BbsI 157 GAAGAC GAAGAC(2/6) BfrI 414 CTTAAG C^TTAAG BpmI 148 CTGGAG CTGGAG(16/14) BpuAI 157 GAAGAC GAAGAC(2/6) BsaHI 4 GACGCC G[GA]^CG[CT]C BsaJI 402 CCGAGG C^C[ACGT][ACGT]GG BseRI 39 CTCCTC GAGGAG(10/8) BsiYI 402 CCGAGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 402 CCGAGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BssKI 147 CCTGG ^CC[ACGT]GG BstNI 147 CCTGG CC^[AT]GG Cac8I 462 GCCAGC GC[ACGT]^[ACGT]GC CfoI 28 GCGC GCG^C DraI 361 TTTAAA TTT^AAA EarI 166 GAAGAG CTCTTC(1/4) EcoRII 147 CCTGG ^CC[AT]GG HaeIII 400 GGCC GG^CC HgaI 4 GACGC GACGC(5/10) HhaI 28 GCGC GCG^C Hin1I 4 GACGCC G[GA]^CG[CT]C HinP1I 28 GCGC G^CGC HinfI 252 GATTC G^A[ACGT]TC Ksp632I 166 GAAGAG CTCTTC(1/4) MvaI 147 CCTGG CC^[AT]GG NspI 329 ACATGC [GA]CATG^[CT] PstI 244 CTGCAG CTGCA^G PvuII 238 CAGCTG CAG^CTG RsaI 141 GTAC GT^AC ScaI 140 AGTACT AGT^ACT ScrFI 147 CCTGG CC^[ACGT]GG SmlI 414 CTTAAG C^T[CT][GA]AG TaqI 326 TCGA T^CGA TfiI 252 GATTC G^A[AT]TC TspRI 420 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 326 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AcsI 58 GAATTT [GA]^AATT[CT] 126 AAATTT ApoI 58 GAATTT [GA]^AATT[CT] 126 AAATTT BsrI 408 ACTGG ACTGG(1/-1) 111 CCAGT BstF5I 99 GGATG GGATG(2/0) 303 GGATG FokI 99 GGATG GGATG(9/13) 303 GGATG MaeIII 317 GTGAC ^GT[ACGT]AC 433 GTGAC MslI 90 CATTATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 298 CATAAGGATG MspA1I 238 CAGCTG C[AC]G^C[GT]G 247 CAGCGG MwoI 46 GCCTCTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 240 GCTGCTGCAGC SfaNI 52 GATGC GCATC(5/9) 64 GATGC SfcI 244 CTGCAG C^T[GA][CT]AG 381 CTACAG Tsp45I 317 GTGAC ^GT[GC]AC 433 GTGAC Tsp509I 59 AATT ^AATT 127 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AciI AluI BbvI DdeI Fnu4HI HphI ItaI MboII MnlI MseI NlaIII Tru9I TseI