Restriction map for
pFN21AE2628
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MseI
MslI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SfcI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflIII 233 ACGTGT A^C[GA][CT]GT
Asp700I 1326 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 1743 GGGCTC G[GA]GC[CT]^C
BbeI 138 GGCGCC GGCGC^C
BbsI 768 GAAGAC GAAGAC(2/6)
BbvCI 1731 CCTCAGC CCTCAGC(-5/-2)
BciVI 1170 GTATCC GTATCC(6/5)
BfaI 1156 CTAG C^TAG
BglI 696 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1548 CTGGAG CTGGAG(16/14)
BpuAI 768 GAAGAC GAAGAC(2/6)
BsaI 169 GAGACC GGTCTC(1/5)
BsaWI 1322 ACCGGA [AT]^CCGG[AT]
BsmBI 1065 CGTCTC CGTCTC(1/5)
BspHI 681 TCATGA T^CATGA
BsrFI 624 GCCGGC [GA]^CCGG[CT]
BssHII 79 GCGCGC G^CGCGC
BssSI 664 CACGAG CACGAG(-5/-1)
BstAPI 705 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1198 GGATCT [GA]^GATC[CT]
Cfr10I 624 GCCGGC [GA]^CCGG[CT]
DraII 1307 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1795 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1300 GACTTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1307 AGGTCCT [GA]G^G[ACGT]CC[CT]
HincII 1120 GTCAAC GT[CT]^[GA]AC
HindII 1120 GTCAAC GT[CT]^[GA]AC
HinfI 678 GATTC G^A[ACGT]TC
KasI 138 GGCGCC G^GCGCC
MaeI 1156 CTAG C^TAG
MflI 1198 GGATCT [GA]^GATC[CT]
MspA1I 672 CAGCGG C[AC]G^C[GT]G
NaeI 624 GCCGGC GCC^GGC
NarI 138 GGCGCC GG^CGCC
NgoMIV 624 GCCGGC G^CCGGC
PpuMI 1307 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 52 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 681 TCATGA T^CATGA
ScaI 602 AGTACT AGT^ACT
SexAI 1796 ACCTGGT A^CC[AT]GGT
SfoI 138 GGCGCC GGC^GCC
SmaI 164 CCCGGG CCC^GGG
TfiI 678 GATTC G^A[AT]TC
XhoII 1198 GGATCT [GA]^GATC[CT]
XmaCI 164 CCCGGG C^CCGGG
XmaI 164 CCCGGG C^CCGGG
XmnI 1326 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 1000 AAATTT [GA]^AATT[CT]
1394 GAATTT
AlwNI 127 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1306 CAGGTCCTG
ApoI 1000 AAATTT [GA]^AATT[CT]
1394 GAATTT
AspHI 826 GTGCTC G[AT]GC[AT]^C
1789 GAGCAC
AvaI 34 CCCGAG C^[CT]CG[GA]G
164 CCCGGG
BcgI 66 GCAGCCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
179 GCAAGAAGATCG
BseRI 492 CTCCTC GAGGAG(10/8)
568 CTCCTC
BsiHKAI 826 GTGCTC G[AT]GC[AT]^C
1789 GAGCAC
BsmFI 348 GGGAC GGGAC(10/14)
1811 GGGAC
BsoBI 34 CCCGAG C^[CT]CG[GA]G
164 CCCGGG
BstXI 1661 CCAACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1694 CCATGGTCCTGG
EarI 487 CTCTTC CTCTTC(1/4)
1330 CTCTTC
Ksp632I 487 CTCTTC CTCTTC(1/4)
1330 CTCTTC
NcoI 837 CCATGG C^CATGG
1694 CCATGG
TaqI 188 TCGA T^CGA
890 TCGA
Tsp509I 1001 AATT ^AATT
1395 AATT
TthHB8I 188 TCGA T^CGA
890 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BalI BanI BbvI
BcnI BmyI BsaHI BsaJI BsgI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp45I TspRI Van91I