Restriction map for
pFN21AE2628
(Circular) (Six-base) MAPSORT of: pFN21AE2628.seq Check: 7855 from: 1 to: 6556
pFN21AE2628 6556 bp
With 182 enzymes: SgfI *
June 10, 2016 17:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2020 2020
Size: 6556
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4366 278
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3899 1937
Size: 1962 4594
Acc65I G'GTAC_C
Cuts at: 3877 3877
Size: 6556
AclI AA'CG_TT
Cuts at: 1563 4683 5056 1563
Size: 3120 373 3063
Fragments arranged by size:
3120 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3338 3340 4619 6003 6271 1632
Size: 1706 2 1279 1384 268 1917
Fragments arranged by size:
1917 1706 1384 1279 268 2
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6359
AflIII A'CryG_T
Cuts at: 1203 2238 5455 6432 1203
Size: 1035 3217 977 1327
Fragments arranged by size:
3217 1327 1035 977
AgeI A'CCGG_T
Cuts at: 5387 5387
Size: 6556
AhdI GACnn_n'nnGTC
Cuts at: 5285 5285
Size: 6556
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6556
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6556
AlwNI CAG_nnn'CTG
Cuts at: 2137 3316 4299 5871 2137
Size: 1179 983 1572 2822
Fragments arranged by size:
2822 1572 1179 983
ApaLI G'TGCA_C
Cuts at: 4613 5769 4613
Size: 1156 5400
ApoI r'AATT_y
Cuts at: 1493 3005 3399 3865 4091 4243 6352 1493
Size: 1512 394 466 226 152 2109 1697
Fragments arranged by size:
2109 1697 1512 466 394 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5110 160
Size: 872 4078 1606
Fragments arranged by size:
4078 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 2169 3881 1870
Size: 73 96 130 1712 4545
Fragments arranged by size:
4545 1712 130 96 73
BamHI G'GATC_C
Cuts at: 3886 4253 3886
Size: 367 6189
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2143 2279 3437 3709 3877
Size: 325 217 627 356 136 1158 272 168
Cuts at: 3877 5332 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1158 627 356 325 272 217
168 136
BanII G_rGCy'C
Cuts at: 1391 3752 3875 1391
Size: 2361 123 4072
Fragments arranged by size:
4072 2361 123
BbeI G_GCGC'C
Cuts at: 2147 2147
Size: 6556
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2780 928
Size: 971 881 4704
Fragments arranged by size:
4704 971 881
BbvCI CC'TCA_GC
Cuts at: 3737 3737
Size: 6556
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2060 2094 2173 2207 4747
Size: 34 50 34 39 34 79 34 2540
Cuts at: 4747 4781 1903
Size: 34 3678
Fragments arranged by size:
3678 2540 79 50 39 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3186 4448 5658 3186
Size: 1262 1210 4084
Fragments arranged by size:
4084 1262 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6556
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2707 5167 136
Size: 107 122 71 631 605 1035 2460 1525
Fragments arranged by size:
2460 1525 1035 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6551 6551
Size: 6556
BlpI GC'TnA_GC
Cuts at: 3973 3973
Size: 6556
Bme1580I G_kGCm'C
Cuts at: 1790 3440 3714 4617 5773 1790
Size: 1650 274 903 1156 2573
Fragments arranged by size:
2573 1650 1156 903 274
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6556
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2698 3679 4287
Size: 416 53 83 399 24 1281 981 608
Cuts at: 4287 5245 442
Size: 958 1753
Fragments arranged by size:
1753 1281 981 958 608 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3574 5216 1239
Size: 2335 1642 2579
Fragments arranged by size:
2579 2335 1642
Bpu10I CC'TnA_GC
Cuts at: 3737 3737
Size: 6556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4681 5546 5844 4681
Size: 865 298 5393
Fragments arranged by size:
5393 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2168 5219 882
Size: 617 669 3051 2219
Fragments arranged by size:
3051 2219 669 617
BsaAI yAC'GTr
Cuts at: 493 1204 4347 493
Size: 711 3143 2702
Fragments arranged by size:
3143 2702 711
BsaBI GATnn'nnATC
Cuts at: 6550 6550
Size: 6556
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2127 2144 2847 3348
Size: 53 83 186 936 594 17 703 501
Cuts at: 3348 4363 4745 5380 275
Size: 1015 382 635 1451
Fragments arranged by size:
1451 1015 936 703 635 594 501 382
186 83 53 17
BsaWI w'CCGG_w
Cuts at: 3327 4989 5387 5661 5808 6219 3327
Size: 1662 398 274 147 411 3664
Fragments arranged by size:
3664 1662 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2480 2510 1178
Size: 30 165 30 1077 30 5224
Fragments arranged by size:
5224 1077 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2488 2564 1186
Size: 1302 76 5178
Fragments arranged by size:
5178 1302 76
BseYI C'CCAG_C
Cuts at: 3247 5759 6255 3247
Size: 2512 496 3548
Fragments arranged by size:
3548 2512 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2054 2645 2873 1163
Size: 573 318 591 228 4846
Fragments arranged by size:
4846 591 573 318 228
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4006 4767 5795 1540
Size: 275 186 2005 761 1028 2301
Fragments arranged by size:
2301 2005 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2835 3798 4617 4702 5773 2835
Size: 963 819 85 1071 3618
Fragments arranged by size:
3618 1071 963 819 85
BsmI GAATG_Cn'
Cuts at: 4067 4160 4067
Size: 93 6463
BsmBI CGTCTCn'nnnn_
Cuts at: 3076 3076
Size: 6556
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2835 3440 3714 3752 3798 3875 4617
Size: 399 1045 605 274 38 46 77 742
Cuts at: 4617 4702 5773 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1045 742 605 399 274 85
77 46 38
BspEI T'CCGG_A
Cuts at: 6219 6219
Size: 6556
BspHI T'CATG_A
Cuts at: 2686 4445 6175 2686
Size: 1759 1730 3067
Fragments arranged by size:
3067 1759 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2020 2523 2895 3911 844
Size: 1176 503 372 1016 3489
Fragments arranged by size:
3489 1176 1016 503 372
BsrBI CCG'CTC
Cuts at: 1394 1823 4443 1394
Size: 429 2620 3507
Fragments arranged by size:
3507 2620 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5051 5225 65
Size: 1183 3803 174 1396
Fragments arranged by size:
3803 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2629 5200 5387 1700
Size: 110 819 2571 187 2869
Fragments arranged by size:
2869 2571 819 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6556
BssHII G'CGCG_C
Cuts at: 1925 2084 1925
Size: 159 6397
BssSI C'ACGA_G
Cuts at: 2669 4616 5628 2669
Size: 1947 1012 3597
Fragments arranged by size:
3597 1947 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2716 794
Size: 1922 4634
BstBI TT'CG_AA
Cuts at: 5360 5360
Size: 6556
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6556
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3673 3706 2011
Size: 1662 33 4861
Fragments arranged by size:
4861 1662 33
BstYI r'GATC_y
Cuts at: 1972 3203 3886 4253 4638 4655 6107 6193 6205
Size: 1231 683 367 385 17 1452 86 12
Cuts at: 6205 6551 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1231 683 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2842 2919 3699 513
Size: 552 1777 77 780 3370
Fragments arranged by size:
3370 1777 780 552 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2299 2455 3670 487
Size: 683 56 1073 156 1215 3373
Fragments arranged by size:
3373 1215 1073 683 156 56
BtsI GCAGTG_nn'
Cuts at: 2229 4072 4866 4886 2229
Size: 1843 794 20 3899
Fragments arranged by size:
3899 1843 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6556
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6521
DraI TTT'AAA
Cuts at: 3860 3860
Size: 6556
DraIII CAC_nnn'GTG
Cuts at: 3805 3805
Size: 6556
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3311 5563 809
Size: 507 1995 2252 1802
Fragments arranged by size:
2252 1995 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2533 3141
Size: 54 1425 50 275 18 402 301 608
Cuts at: 3141 3849 4003 4892 6521 8
Size: 708 154 889 1629 43
Fragments arranged by size:
1629 1425 889 708 608 402 301 275
154 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4003 1537
Size: 275 2191 4090
Fragments arranged by size:
4090 2191 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2498 3341 4486 1353
Size: 1145 843 1145 3423
Fragments arranged by size:
3423 1145 1145 843
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2069 5143 5517 5663 387
Size: 1064 138 480 3074 374 146 1280
Fragments arranged by size:
3074 1280 1064 480 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3338 3340 3574 4619 5216 6003 6271
Size: 393 1706 2 234 1045 597 787 268
Cuts at: 6271 1239
Size: 1524
Fragments arranged by size:
1706 1524 1045 787 597 393 268 234
2
EcoO109I rG'GnC_Cy
Cuts at: 3313 3313
Size: 6556
EcoRI G'AATT_C
Cuts at: 3865 4243 3865
Size: 378 6178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 6556
FspI TGC'GCA
Cuts at: 5062 5062
Size: 6556
HaeII r_GCGC'y
Cuts at: 1678 2147 2248 3248 5703 1678
Size: 469 101 1000 2455 2531
Fragments arranged by size:
2531 2455 1000 469 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5197 5229 5271 5303 1373
Size: 32 135 32 3625 32 42 32 2626
Fragments arranged by size:
3625 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3127 3900 4146 669
Size: 988 281 1189 773 246 3079
Fragments arranged by size:
3079 1189 988 773 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6556
HpaI GTT'AAC
Cuts at: 4146 4146
Size: 6556
KasI G'GCGC_C
Cuts at: 2143 2143
Size: 6556
KpnI G_GTAC'C
Cuts at: 3881 3881
Size: 6556
MfeI C'AATT_G
Cuts at: 4155 4155
Size: 6556
MluI A'CGCG_T
Cuts at: 6432 6432
Size: 6556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5670 5854 1305
Size: 4365 184 2007
Fragments arranged by size:
4365 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2234 2535 3143 3851 10
Size: 54 1425 343 402 301 608 708 2715
Fragments arranged by size:
2715 1425 708 608 402 343 301 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4514 4873 5032 518
Size: 648 3348 359 159 2042
Fragments arranged by size:
3348 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2679 3972 4649 5797 6042 1606
Size: 1073 1293 677 1148 245 2120
Fragments arranged by size:
2120 1293 1148 1073 677 245
NaeI GCC'GGC
Cuts at: 2631 2631
Size: 6556
NarI GG'CG_CC
Cuts at: 2144 2144
Size: 6556
NcoI C'CATG_G
Cuts at: 513 1065 2842 3699 513
Size: 552 1777 857 3370
Fragments arranged by size:
3370 1777 857 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6556
NgoMIV G'CCGG_C
Cuts at: 2629 2629
Size: 6556
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6556
NotI GC'GGCC_GC
Cuts at: 4003 4003
Size: 6556
NspI r_CATG'y
Cuts at: 1132 1229 3914 5459 1132
Size: 97 2685 1545 2229
Fragments arranged by size:
2685 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3660 1348
Size: 2312 4244
PciI A'CATG_T
Cuts at: 5455 5455
Size: 6556
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2241 2385 2925 3805 1108
Size: 1133 144 540 880 3859
Fragments arranged by size:
3859 1133 880 540 144
PfoI T'CCnGG_A
Cuts at: 3417 3417
Size: 6556
PmeI GTTT'AAAC
Cuts at: 3860 3860
Size: 6556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4625 4657 6138 6170 4625
Size: 32 1481 32 5011
Fragments arranged by size:
5011 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3313 3313
Size: 6556
PshAI GACnn'nnGTC
Cuts at: 1613 2061 1613
Size: 448 6108
PsiI TTA'TAA
Cuts at: 4126 4126
Size: 6556
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2288 2320 2288
Size: 32 6524
PstI C_TGCA'G
Cuts at: 830 3908 830
Size: 3078 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6556
SalI G'TCGA_C
Cuts at: 1936 3898 1936
Size: 1962 4594
SbfI CC_TGCA'GG
Cuts at: 3908 3908
Size: 6556
ScaI AGT'ACT
Cuts at: 2609 4804 6516 2609
Size: 2195 1712 2649
Fragments arranged by size:
2649 2195 1712
SexAI A'CCwGG_T
Cuts at: 3801 3801
Size: 6556
SfcI C'TryA_G
Cuts at: 826 1045 3904 5039 5720 5911 826
Size: 219 2859 1135 681 191 1471
Fragments arranged by size:
2859 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2145 2145
Size: 6556
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6556
SmaI CCC'GGG
Cuts at: 1872 2171 3883 1872
Size: 299 1712 4545
Fragments arranged by size:
4545 1712 299
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4660 5561 5823 820
Size: 197 926 2717 901 262 1553
Fragments arranged by size:
2717 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4347 493
Size: 3854 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6556
SphI G_CATG'C
Cuts at: 1132 3914 1132
Size: 2782 3774
SspI AAT'ATT
Cuts at: 5 52 4480 5
Size: 47 4428 2081
Fragments arranged by size:
4428 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6556
StyI C'CwwG_G
Cuts at: 513 1065 2842 2958 3097 3390 3699 3995 513
Size: 552 1777 116 139 293 309 296 3074
Fragments arranged by size:
3074 1777 552 309 296 293 139 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2540 3661 4583 4600 4753 4938 5383
Size: 1255 960 1121 922 17 153 185 445
Cuts at: 5383 325
Size: 1498
Fragments arranged by size:
1498 1255 1121 960 922 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2358 2607 2616
Size: 274 80 33 51 1443 381 249 9
Cuts at: 2616 4802 6514 96
Size: 2186 1712 138
Fragments arranged by size:
2186 1712 1443 381 274 249 138 80
51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2515 2547 2775 2807 6516 6548 2515
Size: 32 228 32 3709 32 2523
Fragments arranged by size:
3709 2523 228 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6556
XbaI T'CTAG_A
Cuts at: 3892 3892
Size: 6556
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6556
XmaI C'CCGG_G
Cuts at: 1870 2169 3881 1870
Size: 299 1712 4545
Fragments arranged by size:
4545 1712 299
XmnI GAAnn'nnTTC
Cuts at: 3335 4685 3335
Size: 1350 5206
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4364 276
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII
SacI SacII SanDI SapI SfiI SrfI SwaI XcmI