(Linear) (Six-base) MAPSORT of: KIEE2628.seq Check: 6948 from: 1 to: 1851 KIEE2628.orf With 182 enzymes: SgfI * June 10, 2016 16:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 15 1851 Size: 15 1836 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1333 1335 1851 Size: 1333 2 516 Fragments arranged by size: 1333 516 2 AflIII A'CryG_T Cuts at: 0 233 1851 Size: 233 1618 AlwNI CAG_nnn'CTG Cuts at: 0 132 1311 1851 Size: 132 1179 540 Fragments arranged by size: 1179 540 132 ApoI r'AATT_y Cuts at: 0 1000 1394 1851 Size: 1000 394 457 Fragments arranged by size: 1000 457 394 AvaI C'yCGr_G Cuts at: 0 34 164 1851 Size: 34 130 1687 Fragments arranged by size: 1687 130 34 BanI G'GyrC_C Cuts at: 0 138 274 1432 1704 1851 Size: 138 136 1158 272 147 Fragments arranged by size: 1158 272 147 138 136 BanII G_rGCy'C Cuts at: 0 1747 1851 Size: 1747 104 BbeI G_GCGC'C Cuts at: 0 142 1851 Size: 142 1709 BbsI GAAGACnn'nnnn_ Cuts at: 0 775 1851 Size: 775 1076 BbvCI CC'TCA_GC Cuts at: 0 1732 1851 Size: 1732 119 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 55 89 168 202 1851 Size: 55 34 79 34 1649 Fragments arranged by size: 1649 79 55 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1181 1851 Size: 1181 670 BglI GCCn_nnn'nGGC Cuts at: 0 702 1851 Size: 702 1149 Bme1580I G_kGCm'C Cuts at: 0 1435 1709 1851 Size: 1435 274 142 Fragments arranged by size: 1435 274 142 BmrI ACTGGGnnnn_n' Cuts at: 0 693 1674 1851 Size: 693 981 177 Fragments arranged by size: 981 693 177 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1569 1851 Size: 1569 282 Bpu10I CC'TnA_GC Cuts at: 0 1732 1851 Size: 1732 119 BsaI GGTCTCn'nnnn_ Cuts at: 0 163 1851 Size: 163 1688 BsaHI Gr'CG_yC Cuts at: 0 122 139 842 1343 1851 Size: 122 17 703 501 508 Fragments arranged by size: 703 508 501 122 17 BsaWI w'CCGG_w Cuts at: 0 1322 1851 Size: 1322 529 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 475 505 1851 Size: 475 30 1346 Fragments arranged by size: 1346 475 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 483 559 1851 Size: 483 76 1292 Fragments arranged by size: 1292 483 76 BseYI C'CCAG_C Cuts at: 0 1242 1851 Size: 1242 609 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 640 868 1851 Size: 49 591 228 983 Fragments arranged by size: 983 591 228 49 BsiHKAI G_wGCw'C Cuts at: 0 830 1793 1851 Size: 830 963 58 Fragments arranged by size: 963 830 58 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1071 1851 Size: 1071 780 Bsp1286I G_dGCh'C Cuts at: 0 830 1435 1709 1747 1793 1851 Size: 830 605 274 38 46 58 Fragments arranged by size: 830 605 274 58 46 38 BspHI T'CATG_A Cuts at: 0 681 1851 Size: 681 1170 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 15 518 890 1851 Size: 15 503 372 961 Fragments arranged by size: 961 503 372 15 BsrFI r'CCGG_y Cuts at: 0 624 1851 Size: 624 1227 BssHII G'CGCG_C Cuts at: 0 79 1851 Size: 79 1772 BssSI C'ACGA_G Cuts at: 0 664 1851 Size: 664 1187 BstAPI GCAn_nnn'nTGC Cuts at: 0 711 1851 Size: 711 1140 BstXI CCAn_nnnn'nTGG Cuts at: 0 1668 1701 1851 Size: 1668 33 150 Fragments arranged by size: 1668 150 33 BstYI r'GATC_y Cuts at: 0 1198 1851 Size: 1198 653 BtgI C'CryG_G Cuts at: 0 837 914 1694 1851 Size: 837 77 780 157 Fragments arranged by size: 837 780 157 77 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 294 450 1665 1851 Size: 294 156 1215 186 Fragments arranged by size: 1215 294 186 156 BtsI GCAGTG_nn' Cuts at: 0 224 1851 Size: 224 1627 DraIII CAC_nnn'GTG Cuts at: 0 1800 1851 Size: 1800 51 DrdI GACnn_nn'nnGTC Cuts at: 0 1306 1851 Size: 1306 545 EaeI y'GGCC_r Cuts at: 0 227 528 1136 1844 1851 Size: 227 301 608 708 7 Fragments arranged by size: 708 608 301 227 7 EarI CTCTTCn'nnn_ Cuts at: 0 493 1336 1851 Size: 493 843 515 Fragments arranged by size: 843 515 493 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 64 1851 Size: 64 1787 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1333 1335 1569 1851 Size: 1333 2 234 282 Fragments arranged by size: 1333 282 234 2 EcoO109I rG'GnC_Cy Cuts at: 0 1308 1851 Size: 1308 543 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 207 1851 Size: 207 1644 HaeII r_GCGC'y Cuts at: 0 142 243 1243 1851 Size: 142 101 1000 608 Fragments arranged by size: 1000 608 142 101 HincII GTy'rAC Cuts at: 0 1122 1851 Size: 1122 729 KasI G'GCGC_C Cuts at: 0 138 1851 Size: 138 1713 MscI TGG'CCA Cuts at: 0 229 530 1138 1846 1851 Size: 229 301 608 708 5 Fragments arranged by size: 708 608 301 229 5 MspA1I CmG'CkG Cuts at: 0 674 1851 Size: 674 1177 NaeI GCC'GGC Cuts at: 0 626 1851 Size: 626 1225 NarI GG'CG_CC Cuts at: 0 139 1851 Size: 139 1712 NcoI C'CATG_G Cuts at: 0 837 1694 1851 Size: 837 857 157 Fragments arranged by size: 857 837 157 NgoMIV G'CCGG_C Cuts at: 0 624 1851 Size: 624 1227 PasI CC'CwG_GG Cuts at: 0 1655 1851 Size: 1655 196 PflMI CCAn_nnn'nTGG Cuts at: 0 236 380 920 1800 1851 Size: 236 144 540 880 51 Fragments arranged by size: 880 540 236 144 51 PfoI T'CCnGG_A Cuts at: 0 1412 1851 Size: 1412 439 PpuMI rG'GwC_Cy Cuts at: 0 1308 1851 Size: 1308 543 PshAI GACnn'nnGTC Cuts at: 0 56 1851 Size: 56 1795 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 283 315 1851 Size: 283 32 1536 Fragments arranged by size: 1536 283 32 ScaI AGT'ACT Cuts at: 0 604 1851 Size: 604 1247 SexAI A'CCwGG_T Cuts at: 0 1796 1851 Size: 1796 55 SfoI GGC'GCC Cuts at: 0 140 1851 Size: 140 1711 SmaI CCC'GGG Cuts at: 0 166 1851 Size: 166 1685 StyI C'CwwG_G Cuts at: 0 837 953 1092 1385 1694 1851 Size: 837 116 139 293 309 157 Fragments arranged by size: 837 309 293 157 139 116 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 535 1656 1851 Size: 535 1121 195 Fragments arranged by size: 1121 535 195 TatI w'GTAC_w Cuts at: 0 353 602 611 1851 Size: 353 249 9 1240 Fragments arranged by size: 1240 353 249 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 510 542 770 802 1851 Size: 510 32 228 32 1049 Fragments arranged by size: 1049 510 228 32 32 XmaI C'CCGG_G Cuts at: 0 164 1851 Size: 164 1687 XmnI GAAnn'nnTTC Cuts at: 0 1330 1851 Size: 1330 521 Enzymes that do cut: AarI AcuI AflIII AlwNI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoO109I FalI HaeII HincII KasI MscI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI PpuMI PshAI PsrI ScaI SexAI SfoI SmaI StyI TaqII TatI TstI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsiEI BsiWI BsmI BspEI BsrBI BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI MslI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI ZraI